FastQCFastQC Report
Sat 18 Jun 2016
SRR3550028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136564
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT49260.43341158087006104Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC40010.35202593078788347Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37350.3286220573588465No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC35590.3131367877215889Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC19620.17262556266079165No Hit
CGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTG16420.144470526956687No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC16400.14429455798353635Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC13650.1200988241753214No Hit
ACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC12280.1080449495145016Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC11400.10030231469587282No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA207.033253E-445.04
CTAAGCG207.033253E-445.01
TATGCGA207.033253E-445.023
ACGTCAC207.033253E-445.021
ATAACGC351.2119199E-745.011
TCGACGG406.8157533E-945.02
GTACGAG551.8189894E-1245.01
TAAACGG900.042.52
TCACGAC850.039.70588325
CGGTCTA850.039.70588331
CACGACC1900.039.07894527
AACGGGA5400.038.754
CTATACG356.2482286E-638.5714261
GTATGCG356.2482286E-638.5714261
CGTTTTT20300.038.2389141
ACACGAC1900.037.89473726
ACGGGAG8550.037.631585
CGTTATT3050.037.622951
ACGGGTA900.037.55
ATATGCG301.1399139E-437.4999961
Produced by FastQC (version 0.11.5)