##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1136564
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91797470270042	33.0	31.0	34.0	30.0	34.0
2	32.00425317008105	33.0	31.0	34.0	30.0	34.0
3	31.97850714961938	33.0	31.0	34.0	30.0	34.0
4	35.71334302335812	37.0	35.0	37.0	33.0	37.0
5	31.574750739949533	37.0	35.0	37.0	0.0	37.0
6	33.26376077370038	37.0	35.0	37.0	17.0	37.0
7	35.215741480462164	37.0	35.0	37.0	32.0	37.0
8	35.40103065027574	37.0	35.0	37.0	33.0	37.0
9	37.42092394269042	39.0	37.0	39.0	35.0	39.0
10	37.058410261102765	39.0	37.0	39.0	33.0	39.0
11	37.11318852259969	39.0	37.0	39.0	34.0	39.0
12	37.08398999088481	39.0	37.0	39.0	34.0	39.0
13	36.96495225961758	39.0	37.0	39.0	33.0	39.0
14	37.907319781376145	40.0	37.0	41.0	33.0	41.0
15	38.11985774668211	40.0	37.0	41.0	33.0	41.0
16	38.03221024069036	40.0	37.0	41.0	33.0	41.0
17	38.05460669174811	40.0	37.0	41.0	33.0	41.0
18	38.05123600606741	40.0	37.0	41.0	33.0	41.0
19	38.012750711794496	40.0	37.0	41.0	33.0	41.0
20	38.072096247989556	40.0	37.0	41.0	34.0	41.0
21	37.94083923122675	40.0	37.0	41.0	33.0	41.0
22	37.958373659556344	40.0	37.0	41.0	33.0	41.0
23	37.733815253694466	39.0	36.0	41.0	33.0	41.0
24	37.48689823010407	39.0	36.0	41.0	32.0	41.0
25	37.32234260455196	39.0	35.0	41.0	32.0	41.0
26	37.61358709232388	39.0	36.0	41.0	33.0	41.0
27	37.77015988540901	39.0	36.0	41.0	33.0	41.0
28	37.706126535769215	40.0	36.0	41.0	33.0	41.0
29	37.70998905472987	40.0	36.0	41.0	33.0	41.0
30	37.57237163943253	40.0	36.0	41.0	33.0	41.0
31	37.57044389933167	40.0	36.0	41.0	33.0	41.0
32	37.4820854786884	40.0	36.0	41.0	33.0	41.0
33	37.46386037213919	40.0	36.0	41.0	33.0	41.0
34	37.37421825783678	40.0	36.0	41.0	33.0	41.0
35	37.320487891574956	40.0	36.0	41.0	33.0	41.0
36	37.23082202146118	39.0	35.0	41.0	32.0	41.0
37	37.23807986175878	39.0	35.0	41.0	32.0	41.0
38	37.105738876121364	39.0	35.0	41.0	32.0	41.0
39	37.021845668171785	39.0	35.0	41.0	32.0	41.0
40	36.93435389472128	39.0	35.0	41.0	32.0	41.0
41	36.83795721138449	39.0	35.0	41.0	31.0	41.0
42	36.86484791001651	39.0	35.0	41.0	31.0	41.0
43	36.792187681468	39.0	35.0	41.0	31.0	41.0
44	36.74440066727435	39.0	35.0	41.0	31.0	41.0
45	36.65685258375243	39.0	35.0	40.0	31.0	41.0
46	36.556020602447376	39.0	35.0	40.0	31.0	41.0
47	36.51308857222295	39.0	35.0	40.0	31.0	41.0
48	36.46061286473969	39.0	35.0	40.0	31.0	41.0
49	36.43728025874478	39.0	35.0	40.0	31.0	41.0
50	36.32352159667207	38.0	35.0	40.0	31.0	41.0
51	35.222027092183104	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	20.0
10	26.0
11	34.0
12	31.0
13	29.0
14	36.0
15	53.0
16	93.0
17	165.0
18	292.0
19	525.0
20	884.0
21	1382.0
22	2108.0
23	3053.0
24	4461.0
25	5772.0
26	7364.0
27	8982.0
28	10600.0
29	13023.0
30	16853.0
31	22513.0
32	31445.0
33	46000.0
34	73317.0
35	86618.0
36	108993.0
37	169242.0
38	252916.0
39	269641.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40198000288589	21.46267170172555	24.54837563040885	12.586972664979712
2	33.40938125789661	24.368183401902577	24.63468841173924	17.587746928461574
3	31.87633956380811	23.88585244649663	26.872221889836386	17.365586099858874
4	28.75060269373304	24.99454496183233	26.50330293762604	19.751549406808593
5	22.055951094702984	37.19517774625978	22.414135939551137	18.334735219486102
6	25.699564652760426	33.336794056471966	25.085432936464642	15.87820835430297
7	77.71986443350308	5.987696249397306	10.476928707930218	5.815510609169391
8	76.33243706469676	5.655290859115721	11.003603844570126	7.008668231617402
9	69.58059554939273	7.883673950609029	13.995692279537272	8.54003822046097
10	36.675101446113025	26.587064168845746	20.060111001228265	16.677723383812967
11	28.680303088959352	23.8970264762917	25.916446412168607	21.50622402258034
12	25.484090645137446	20.82249657740347	31.493431078232287	22.199981699226793
13	24.87778954814687	21.56024649733759	33.431641333000165	20.130322621515376
14	20.341045466863285	25.980059195962568	31.567865953875014	22.111029383299137
15	19.023741733857484	24.052583048556876	35.193618661157664	21.73005655642797
16	21.89142010480712	25.572163116199352	30.05330100196733	22.483115777026192
17	22.074339852397227	25.01240581260712	28.48268993211117	24.43056440288448
18	22.909752552429953	23.824175321407328	30.307136245737155	22.958935880425564
19	22.53634639140427	26.41047930428907	27.966484949373726	23.086689354932936
20	23.075603309624448	26.989945132874173	27.87110976592607	22.063341791575308
21	22.130825892778585	27.04968659925882	29.86519016966928	20.954297338293312
22	20.21399586824851	24.16951443121549	28.80260152529906	26.813888175236944
23	20.34465283081287	25.781038287329178	29.521346796132907	24.352962085725043
24	21.96805459261423	24.627121745893767	28.830316638570284	24.57450702292172
25	22.236231307695828	26.424116899708245	26.068923527403648	25.270728265192282
26	20.48419622652134	26.149957239539525	27.13309589253223	26.232750641406906
27	22.19003945224378	24.497696566141457	28.32027056989312	24.991993411721644
28	19.895931949278705	25.50775847202621	28.23061437807286	26.365695200622223
29	21.909808862501365	26.020532059787215	26.653932378643	25.41572669906842
30	23.50065636426985	25.308385625446522	26.74446841532901	24.446489594954617
31	22.982075800394874	25.953927803449695	26.812304454478586	24.251691941676842
32	24.330877979594636	26.52186766429343	25.22550423909256	23.921750117019368
33	23.21224321727593	25.178520523261337	25.06502053557917	26.544215723883564
34	21.043161669734392	25.042056584583005	29.750282430201906	24.164499315480693
35	23.443202494536163	25.00624689854685	26.42473279111427	25.12581781580272
36	22.05243171523997	27.11558697970374	26.117754917452956	24.71422638760334
37	22.019173579314497	25.070387589260264	29.146004976402562	23.76443385502268
38	24.144702806001245	25.705899535793847	26.42024558229893	23.729152075905976
39	23.680232701370095	23.6281458853175	27.178935810037974	25.51268560327443
40	23.786781914612813	23.390851725023847	30.144013007626498	22.678353352736845
41	21.352075202100366	23.6548931692364	27.33590013408836	27.657131494574877
42	22.57602739484974	23.710587349238583	28.190141514248207	25.52324374166347
43	22.88881224462503	21.870655765975343	29.116706142372976	26.123825847026655
44	22.694366529293557	22.780943264083678	28.09450237734083	26.43018782928194
45	23.160948261602517	21.378822486019263	28.16189849405753	27.298330758320695
46	23.597175346042988	22.941602936570224	28.279973675041614	25.18124804234517
47	20.650926828581582	23.167899036041963	31.03186446165812	25.149309673718328
48	21.08688995956233	22.80461109097244	29.766647544704917	26.341851404760313
49	23.176169577780044	21.15094266578917	30.575224976332173	25.097662780098613
50	21.23883916787792	20.780791930766767	30.779700923133234	27.200667978222082
51	21.15921320752725	20.762403173072524	28.403679863166527	29.6747037562337
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	610.0
1	1096.0
2	1582.0
3	4130.5
4	6679.0
5	4400.5
6	2122.0
7	2075.5
8	2029.0
9	1994.5
10	1960.0
11	2107.0
12	2254.0
13	2289.0
14	2324.0
15	2344.0
16	2364.0
17	2366.5
18	2369.0
19	2470.0
20	2571.0
21	2807.0
22	3043.0
23	3331.0
24	3619.0
25	4620.5
26	6783.0
27	7944.0
28	9465.5
29	10987.0
30	12805.5
31	14624.0
32	16014.5
33	17405.0
34	19425.0
35	21445.0
36	24163.0
37	26881.0
38	29363.5
39	31846.0
40	35951.0
41	40056.0
42	46718.5
43	53381.0
44	62230.0
45	71079.0
46	90088.5
47	109098.0
48	124740.0
49	140382.0
50	135587.5
51	130793.0
52	110090.0
53	89387.0
54	77979.5
55	66572.0
56	60833.5
57	55095.0
58	51397.5
59	47700.0
60	44177.0
61	40654.0
62	37210.0
63	33766.0
64	29938.0
65	26110.0
66	22733.0
67	19356.0
68	16835.5
69	14315.0
70	12061.5
71	9808.0
72	8460.5
73	7113.0
74	6065.0
75	4004.5
76	2992.0
77	2393.0
78	1794.0
79	1364.5
80	935.0
81	725.0
82	515.0
83	372.0
84	229.0
85	141.5
86	54.0
87	52.5
88	51.0
89	33.5
90	16.0
91	11.5
92	7.0
93	6.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1136564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.32084855110466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.38333891945425	25.21320103046218
2	11.274871230280231	7.964760383158702
3	4.327578948077095	4.5856128185393965
4	2.3752366896968606	3.3558150155923996
5	1.4912031057610746	2.6335279528761912
6	1.0961812221440497	2.3230830559148874
7	0.8451524449086925	2.089605105645102
8	0.6994321664625057	1.9763630098714549
9	0.6042437701938105	1.920816242546766
>10	5.7349223650689165	37.99959741051052
>50	0.10477454341258702	2.483234147165004
>100	0.0549272275344949	3.744796314340435
>500	0.0050858543784246864	1.2536844752358565
>1k	0.003051512627054812	2.4559030381411393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	4926	0.43341158087006104	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	4001	0.35202593078788347	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3735	0.3286220573588465	No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	3559	0.3131367877215889	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC	1962	0.17262556266079165	No Hit
CGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTG	1642	0.144470526956687	No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1640	0.14429455798353635	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	1365	0.1200988241753214	No Hit
ACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1228	0.1080449495145016	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	1140	0.10030231469587282	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.759689731506541E-4	0.0	0.0	0.45224026099718095	0.0
2	1.759689731506541E-4	0.0	0.0	1.412414962993725	0.0
3	1.759689731506541E-4	0.0	0.0	2.1178745763546973	0.0
4	1.759689731506541E-4	0.0	0.0	3.0156682773693344	0.0
5	1.759689731506541E-4	0.0	0.0	5.035176197732816	0.0
6	1.759689731506541E-4	0.0	0.0	6.68013415874513	0.0
7	1.759689731506541E-4	0.0	0.0	8.192147560542125	0.0
8	1.759689731506541E-4	0.0	0.0	10.56517714796527	0.0
9	1.759689731506541E-4	0.0	0.0	11.543300685223182	0.0
10	1.759689731506541E-4	0.0	0.0	13.396165988012994	0.0
11	1.759689731506541E-4	0.0	0.0	15.677427755938073	0.0
12	1.759689731506541E-4	0.0	0.0	17.550793444099938	0.0
13	1.759689731506541E-4	0.0	0.0	18.32100963958035	0.0
14	1.759689731506541E-4	0.0	0.0	18.64672820888221	0.0
15	1.759689731506541E-4	0.0	0.0	19.213788224860192	0.0
16	1.759689731506541E-4	0.0	0.0	20.233440439781656	0.0
17	1.759689731506541E-4	0.0	0.0	21.401874421502	0.0
18	1.759689731506541E-4	0.0	0.0	22.66207622272041	0.0
19	1.759689731506541E-4	0.0	0.0	23.44892148616356	0.0
20	1.759689731506541E-4	0.0	0.0	24.24086984982808	0.0
21	1.759689731506541E-4	0.0	0.0	25.13048099359121	0.0
22	1.759689731506541E-4	0.0	0.0	26.051678568034884	0.0
23	1.759689731506541E-4	0.0	0.0	26.913222660580487	0.0
24	1.759689731506541E-4	0.0	0.0	27.632319869360636	0.0
25	1.759689731506541E-4	0.0	0.0	28.248387244361073	0.0
26	1.759689731506541E-4	0.0	0.0	28.81465539995988	0.0
27	1.759689731506541E-4	0.0	0.0	29.442688665134565	0.0
28	1.759689731506541E-4	0.0	0.0	30.04582232060843	0.0
29	2.639534597259811E-4	0.0	0.0	30.694004033208866	0.0
30	2.639534597259811E-4	0.0	0.0	31.416268683505724	0.0
31	2.639534597259811E-4	0.0	0.0	31.99027947392316	0.0
32	2.639534597259811E-4	0.0	0.0	32.59103754825949	0.0
33	2.639534597259811E-4	0.0	0.0	33.16381655586487	0.0
34	3.519379463013082E-4	0.0	0.0	33.73624362552395	0.0
35	3.519379463013082E-4	0.0	0.0	34.32283619752165	0.0
36	3.519379463013082E-4	0.0	0.0	34.852502806705125	0.0
37	3.519379463013082E-4	0.0	0.0	35.42299421765954	0.0
38	3.519379463013082E-4	0.0	0.0	36.01512981231149	0.0
39	3.519379463013082E-4	0.0	0.0	36.79564019272122	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTA	20	7.033253E-4	45.0	4
CTAAGCG	20	7.033253E-4	45.0	1
TATGCGA	20	7.033253E-4	45.0	23
ACGTCAC	20	7.033253E-4	45.0	21
ATAACGC	35	1.2119199E-7	45.0	11
TCGACGG	40	6.8157533E-9	45.0	2
GTACGAG	55	1.8189894E-12	45.0	1
TAAACGG	90	0.0	42.5	2
TCACGAC	85	0.0	39.705883	25
CGGTCTA	85	0.0	39.705883	31
CACGACC	190	0.0	39.078945	27
AACGGGA	540	0.0	38.75	4
CTATACG	35	6.2482286E-6	38.571426	1
GTATGCG	35	6.2482286E-6	38.571426	1
CGTTTTT	2030	0.0	38.238914	1
ACACGAC	190	0.0	37.894737	26
ACGGGAG	855	0.0	37.63158	5
CGTTATT	305	0.0	37.62295	1
ACGGGTA	90	0.0	37.5	5
ATATGCG	30	1.1399139E-4	37.499996	1
>>END_MODULE