FastQCFastQC Report
Sat 18 Jun 2016
SRR3550025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819686
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365182.006829749748033No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC358621.9707795740583816No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC226871.2467535607791675No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG208141.1438237146408776No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT110820.609006169196224No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC84070.4620027850958902No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT79730.43815251642316316No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC62420.3430262144128163No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT59860.3289578531680741No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC57670.316922809759486No Hit
CGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG53220.2924680411895239No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT50700.2786194980892308No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT48290.2653754548861727No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAACTAGT46680.25652777457209647No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT41290.22690727960758064No Hit
CGTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT40670.22350009836861964No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA40430.22218118950192509No Hit
GAATATGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC38880.21366323640452253No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC33000.18134996917050525No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC31870.17514010658981824No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG24030.13205575027779518No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC23490.12908820532773235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG302.1662036E-645.0000041
CCCCGTA207.034372E-445.040
CTCGACG207.034372E-445.01
CGTTATT21050.044.358671
CGTTTTT153600.043.549811
AGTACGG2300.043.0434762
TAATACG6850.042.7007334
CGCATCG501.0822987E-940.521
CGATGAA15800.040.4430419
CCGATGA15900.040.1886818
CGTTCTG8400.040.178571
ACGATAG451.929402E-840.0000041
ATAATAC11400.039.6710553
TACGGCT31150.039.5104337
GATGAAT16550.039.4259820
GTTAACG403.4596997E-739.3751
ACAACGA5550.038.5135113
GTTATTT25050.038.3532942
GTTTTTT178500.037.903362
CGTTTCT10850.037.7419361
Produced by FastQC (version 0.11.5)