##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550025_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1819686
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.818549464028408	31.0	31.0	34.0	30.0	34.0
2	31.946200608236804	33.0	31.0	34.0	30.0	34.0
3	31.8627735774194	33.0	31.0	34.0	30.0	34.0
4	35.64251854440821	37.0	35.0	37.0	33.0	37.0
5	31.48814575701522	37.0	35.0	37.0	0.0	37.0
6	33.15554991355651	37.0	35.0	37.0	17.0	37.0
7	35.09336555867331	37.0	35.0	37.0	32.0	37.0
8	35.20048623773552	37.0	35.0	37.0	32.0	37.0
9	37.285478373741405	39.0	37.0	39.0	34.0	39.0
10	37.05188037936215	39.0	37.0	39.0	33.0	39.0
11	37.0608896260124	39.0	37.0	39.0	33.0	39.0
12	36.99048901843505	39.0	37.0	39.0	33.0	39.0
13	36.840505449841345	39.0	37.0	39.0	33.0	39.0
14	37.810832198522164	40.0	37.0	41.0	33.0	41.0
15	37.90661960360194	40.0	37.0	41.0	33.0	41.0
16	37.9088875773073	40.0	37.0	41.0	33.0	41.0
17	37.81620510351786	40.0	36.0	41.0	33.0	41.0
18	37.69218645414649	39.0	36.0	41.0	33.0	41.0
19	37.55545682057234	39.0	36.0	41.0	33.0	41.0
20	37.36292525193907	39.0	35.0	41.0	32.0	41.0
21	37.33177372359847	39.0	35.0	41.0	33.0	41.0
22	37.374620676314485	39.0	35.0	41.0	33.0	41.0
23	37.124302214777714	39.0	35.0	40.0	32.0	41.0
24	36.864440898045046	39.0	35.0	40.0	32.0	41.0
25	36.74184502161362	38.0	35.0	40.0	31.0	41.0
26	36.998329382102185	39.0	35.0	40.0	32.0	41.0
27	37.10974970406982	39.0	35.0	40.0	33.0	41.0
28	36.98812652292758	39.0	35.0	40.0	32.0	41.0
29	36.97487863290699	39.0	35.0	41.0	32.0	41.0
30	36.737875655470226	39.0	35.0	40.0	31.0	41.0
31	36.514497556171776	39.0	35.0	40.0	31.0	41.0
32	36.19904203252649	38.0	35.0	40.0	30.0	41.0
33	35.98556674063547	39.0	35.0	40.0	30.0	41.0
34	35.68589800657916	38.0	35.0	40.0	27.0	41.0
35	35.51432554847375	38.0	35.0	40.0	26.0	41.0
36	35.329751396669536	38.0	35.0	40.0	25.0	41.0
37	35.22514543717982	38.0	35.0	40.0	25.0	41.0
38	35.02752123168503	38.0	34.0	40.0	24.0	41.0
39	34.995469548042905	38.0	34.0	40.0	24.0	41.0
40	34.892408910108664	38.0	34.0	40.0	23.0	41.0
41	34.72249882672065	38.0	34.0	40.0	23.0	41.0
42	34.72673966827244	38.0	34.0	40.0	23.0	41.0
43	34.69429396060639	38.0	34.0	40.0	23.0	41.0
44	34.67249789249354	38.0	34.0	40.0	23.0	41.0
45	34.601929124035685	38.0	34.0	40.0	23.0	41.0
46	34.54207923784653	37.0	34.0	40.0	23.0	41.0
47	34.37443657861851	37.0	34.0	40.0	23.0	41.0
48	34.33896947055701	37.0	34.0	40.0	23.0	41.0
49	34.31304686632749	37.0	34.0	40.0	23.0	41.0
50	34.16343259221646	37.0	34.0	40.0	22.0	41.0
51	33.14568557432436	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	28.0
10	41.0
11	31.0
12	41.0
13	39.0
14	64.0
15	95.0
16	204.0
17	376.0
18	847.0
19	1725.0
20	3279.0
21	5530.0
22	8147.0
23	11512.0
24	16009.0
25	22959.0
26	30415.0
27	34060.0
28	33910.0
29	34648.0
30	38822.0
31	47486.0
32	61434.0
33	84986.0
34	129437.0
35	150474.0
36	178011.0
37	252229.0
38	345242.0
39	327466.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75221109576048	17.131691951248733	20.03895177519638	20.07714517779441
2	36.82503464883502	28.237619017786585	20.00927632569575	14.928070007682646
3	25.978932629036	28.720724344749588	31.147461704931512	14.152881321282903
4	23.424975517754163	20.85810409048594	38.9757903286611	16.7411300630988
5	23.73618305575797	32.42075830665291	27.210189010631503	16.632869626957618
6	22.693695505708128	32.41531780757779	30.7731114049347	14.117875281779385
7	66.45498179356218	4.790551776515288	23.33122307914662	5.423243350775904
8	64.79090348554641	8.994408925495938	19.480888460976235	6.733799127981421
9	58.996607106940424	5.860571549157382	20.976091479518992	14.166729864383196
10	36.52201533671194	19.405051201141298	27.10791861892656	16.965014843220207
11	28.87685018184456	19.135169474293917	32.10042831565446	19.887552028207065
12	23.30314131119325	16.56016477568108	36.88207745731956	23.25461645580611
13	23.160094653693	17.93721554158245	41.157375503246165	17.745314301478388
14	18.43862072907084	24.130921488652437	36.90499349887838	20.525464283398346
15	16.367274353926998	20.725553749383135	42.681099926031195	20.226071970658673
16	19.529523225435597	22.694354960141474	34.31185380334849	23.464268011074438
17	19.43752933198365	23.87076671469693	34.74148836667425	21.950215586645168
18	20.052910227368898	22.568893754197152	34.32817530057384	23.050020717860114
19	19.634156662193366	25.09652764268121	31.680905386973357	23.588410308152067
20	20.73335729351108	25.459722171847233	33.901233509517574	19.905687025124113
21	19.397357566085578	27.84529858448106	33.805337844001656	18.952006005431706
22	18.27842825630356	21.052917921003953	35.47144946985359	25.1972043528389
23	19.24496863744624	24.597045863956748	34.4771020934381	21.680883405158912
24	21.518108069194355	22.458600000219818	32.40152421901361	23.621767711572218
25	21.075833962562772	27.265967864785463	29.39534623006387	22.262851942587897
26	20.3116911379216	21.429081720692473	32.617715364079295	25.641511777306636
27	23.87950448593878	21.302851151242578	30.626108020834366	24.191536341984275
28	20.431437072110242	23.19026469401864	31.84280145036012	24.535496783511
29	26.11846219622506	21.56965542406767	28.80117778561796	23.51070459408931
30	26.890298655921956	23.17289906060716	29.656435231133283	20.2803670523376
31	24.93144421619994	24.93204871609717	27.68312774841374	22.45337931928915
32	26.088731792188323	27.429897246008377	26.292942848381536	20.188428113421768
33	23.929732931945402	23.913411434720054	26.41598605473692	25.740869578597625
34	23.52730086399522	24.74344474815985	30.54873203398828	21.180522353856652
35	23.522684682961785	27.43989897158081	27.76896673382111	21.268449611636292
36	25.8696830112448	27.92817002493837	26.1736915050179	20.028455458798934
37	23.30775749222668	27.521781230388097	29.75062730602972	19.419833971355498
38	21.990936897904366	27.51112005038232	26.6755912833313	23.822351768382017
39	24.28418968986957	24.371732265896423	29.005608659955616	22.338469384278387
40	22.84262229857239	23.1533902002873	31.685521568006784	22.31846593313352
41	22.448818092791832	24.652494990894034	29.887683919093732	23.0110029972204
42	22.56548657295819	24.969747527870194	31.65650557293951	20.80826032623211
43	25.568037562524527	21.26768024813072	29.658413594433323	23.50586859491143
44	23.729478602352273	21.303510605675925	29.752605669329764	25.214405122642034
45	21.637469321630213	20.41275252983207	29.85542560639583	28.094352542141888
46	25.79192234264593	22.211854133075708	28.617409816858512	23.37881370741985
47	20.510791422256368	21.652252091844417	34.89090975036353	22.946046735535692
48	21.018681245006007	22.84597452527524	30.900386110570725	25.234958119148025
49	21.680718541550576	20.25497805665373	34.3535642962577	23.71073910553799
50	20.912289263092642	19.75456205081536	33.773464213056535	25.55968447303546
51	20.697032345140865	19.29734031036124	31.39156975434223	28.61405759015566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1180.0
1	1856.5
2	2533.0
3	31475.5
4	60418.0
5	38032.5
6	15647.0
7	14851.0
8	14055.0
9	13754.0
10	13453.0
11	13193.0
12	12933.0
13	12295.0
14	11657.0
15	10967.0
16	10277.0
17	9695.0
18	9113.0
19	8542.5
20	7972.0
21	7999.5
22	8027.0
23	8201.0
24	8375.0
25	9043.0
26	10988.5
27	12266.0
28	13948.0
29	15630.0
30	17132.0
31	18634.0
32	20852.0
33	23070.0
34	25337.5
35	27605.0
36	30140.5
37	32676.0
38	35532.5
39	38389.0
40	42934.5
41	47480.0
42	53175.0
43	58870.0
44	78892.5
45	98915.0
46	177111.5
47	255308.0
48	219976.0
49	184644.0
50	180510.5
51	176377.0
52	152047.5
53	127718.0
54	112638.0
55	97558.0
56	90902.0
57	84246.0
58	79296.0
59	74346.0
60	68959.0
61	63572.0
62	58270.5
63	52969.0
64	48072.5
65	43176.0
66	37022.5
67	30869.0
68	26747.0
69	22625.0
70	19548.0
71	16471.0
72	14354.5
73	12238.0
74	9982.5
75	6504.5
76	5282.0
77	4111.0
78	2940.0
79	2173.5
80	1407.0
81	1085.0
82	763.0
83	572.5
84	382.0
85	246.0
86	110.0
87	71.5
88	33.0
89	26.0
90	19.0
91	14.5
92	10.0
93	9.0
94	8.0
95	4.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1819686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.538581869020426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.2585739077214	25.647858343885392
2	9.03599886417761	6.2418117307754795
3	3.6801959592114533	3.8132624829378874
4	2.11923847138766	2.9278196577600157
5	1.518010342581467	2.6214962247634865
6	1.2000619725766601	2.4869063212642275
7	0.9788877814193624	2.3666577045395494
8	0.8740496235870026	2.4150747585476933
9	0.7268254677493788	2.2593168830115884
>10	5.464588034955534	30.970348408483993
>50	0.09032843457368947	2.098414702658197
>100	0.04511405651309373	2.9451112502721655
>500	0.003483035805301554	0.8050302360074143
>1k	0.002653741565944041	2.563287763494468
>5k	0.0011610119351005178	2.5644907546676095
>10k+	8.292942393575127E-4	7.273112776930857
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36518	2.006829749748033	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	35862	1.9707795740583816	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	22687	1.2467535607791675	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	20814	1.1438237146408776	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	11082	0.609006169196224	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	8407	0.4620027850958902	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	7973	0.43815251642316316	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	6242	0.3430262144128163	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	5986	0.3289578531680741	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	5767	0.316922809759486	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	5322	0.2924680411895239	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	5070	0.2786194980892308	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	4829	0.2653754548861727	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAACTAGT	4668	0.25652777457209647	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	4129	0.22690727960758064	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	4067	0.22350009836861964	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA	4043	0.22218118950192509	No Hit
GAATATGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	3888	0.21366323640452253	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3300	0.18134996917050525	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	3187	0.17514010658981824	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	2403	0.13205575027779518	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	2349	0.12908820532773235	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.4954536112274314E-5	0.0	0.0	0.23927205023284237	0.0
2	5.4954536112274314E-5	0.0	0.0	1.013691373127012	0.0
3	5.4954536112274314E-5	0.0	0.0	1.628302905006688	0.0
4	5.4954536112274314E-5	0.0	0.0	2.899456279819705	0.0
5	5.4954536112274314E-5	0.0	0.0	6.367856872009786	0.0
6	5.4954536112274314E-5	0.0	0.0	8.7144155640039	0.0
7	5.4954536112274314E-5	0.0	0.0	10.610896605238485	0.0
8	5.4954536112274314E-5	0.0	0.0	13.025049376650697	0.0
9	5.4954536112274314E-5	0.0	0.0	14.272847073615997	0.0
10	5.4954536112274314E-5	0.0	0.0	17.244348750278895	0.0
11	5.4954536112274314E-5	0.0	0.0	18.929529600161786	0.0
12	5.4954536112274314E-5	0.0	0.0	21.599275919032184	0.0
13	5.4954536112274314E-5	0.0	0.0	22.34028288397009	0.0
14	5.4954536112274314E-5	0.0	0.0	22.75277163202882	0.0
15	5.4954536112274314E-5	0.0	0.0	23.374856980819768	0.0
16	5.4954536112274314E-5	0.0	0.0	24.012659326938824	0.0
17	5.4954536112274314E-5	0.0	0.0	24.65777062636081	0.0
18	5.4954536112274314E-5	0.0	0.0	25.365420187878566	0.0
19	5.4954536112274314E-5	0.0	0.0	26.14720341861178	0.0
20	5.4954536112274314E-5	0.0	0.0	26.697463188703985	0.0
21	5.4954536112274314E-5	0.0	0.0	27.2289834619819	0.0
22	1.0990907222454863E-4	0.0	0.0	27.8718965799594	0.0
23	1.0990907222454863E-4	0.0	0.0	28.39379980941767	0.0
24	1.0990907222454863E-4	0.0	0.0	28.85146118616069	0.0
25	1.6486360833682296E-4	0.0	0.0	29.246529346271828	0.0
26	1.6486360833682296E-4	0.0	0.0	29.630771462768852	0.0
27	1.6486360833682296E-4	0.0	0.0	30.073320342080997	0.0
28	1.6486360833682296E-4	0.0	0.0	30.476906455289537	0.0
29	2.1981814444909726E-4	0.0	0.0	30.904068064490247	0.0
30	2.1981814444909726E-4	0.0	0.0	31.411463296414876	0.0
31	2.1981814444909726E-4	0.0	0.0	31.812136819209467	0.0
32	2.1981814444909726E-4	0.0	0.0	32.23935338294629	0.0
33	2.1981814444909726E-4	0.0	0.0	32.64557731388822	0.0
34	2.747726805613716E-4	0.0	0.0	33.0488886544162	0.0
35	3.297272166736459E-4	0.0	0.0	33.468191764952856	0.0
36	4.396362888981945E-4	0.0	0.0	33.871942741769736	0.0
37	4.396362888981945E-4	0.0	0.0	34.31146912159571	0.0
38	4.945908250104688E-4	0.0	0.0	34.8044113105228	0.0
39	4.945908250104688E-4	0.0	0.0	35.4871664671817	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	30	2.1662036E-6	45.000004	1
CCCCGTA	20	7.034372E-4	45.0	40
CTCGACG	20	7.034372E-4	45.0	1
CGTTATT	2105	0.0	44.35867	1
CGTTTTT	15360	0.0	43.54981	1
AGTACGG	230	0.0	43.043476	2
TAATACG	685	0.0	42.700733	4
CGCATCG	50	1.0822987E-9	40.5	21
CGATGAA	1580	0.0	40.44304	19
CCGATGA	1590	0.0	40.18868	18
CGTTCTG	840	0.0	40.17857	1
ACGATAG	45	1.929402E-8	40.000004	1
ATAATAC	1140	0.0	39.671055	3
TACGGCT	3115	0.0	39.510433	7
GATGAAT	1655	0.0	39.42598	20
GTTAACG	40	3.4596997E-7	39.375	1
ACAACGA	555	0.0	38.51351	13
GTTATTT	2505	0.0	38.353294	2
GTTTTTT	17850	0.0	37.90336	2
CGTTTCT	1085	0.0	37.741936	1
>>END_MODULE