FastQCFastQC Report
Sat 18 Jun 2016
SRR3550024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149931
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126671.101544353530777No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC58990.512987300977189Illumina PCR Primer Index 7 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT45760.39793691969344247Illumina PCR Primer Index 7 (96% over 28bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC45210.3931540240240501No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG41290.35906502216219927No Hit
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG39720.34541202906957025Illumina PCR Primer Index 7 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC38060.33097638032194976Illumina PCR Primer Index 7 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC31290.27210328271870227Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT30330.26375495573212654Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC24970.21714346339041213No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC24850.21609992251709018Illumina PCR Primer Index 7 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT20920.18192395891579582Illumina PCR Primer Index 7 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT20790.18079345630303034Illumina PCR Primer Index 7 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.1474001483567275No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT16790.14600876052563155Illumina PCR Primer Index 7 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT13250.1152243047626336Illumina PCR Primer Index 7 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC12780.1111371030087892Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC12240.10644116907884038Illumina PCR Primer Index 7 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT11600.10087561775445657Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG253.8908078E-545.01
CGATAAC253.8908078E-545.010
CGTTATT8850.043.983051
CGTTTTT60450.043.362281
TAATACG1150.043.043484
CGTTTCT3700.041.9594571
TTACGGG3250.040.1538473
AGTTACG451.9283107E-840.01
TACGGGA4850.039.8969084
TCGTACG403.4582263E-739.37530
CGGTAAT403.4582263E-739.3756
GACGGGA5050.039.2079164
TGTTGCG356.2482904E-638.571431
CGTTCGG1350.038.33333645
ACTACGG659.094947E-1238.076922
TCGACGG659.094947E-1238.076922
CATACGA950.037.89473718
ACGTATG301.13992246E-437.4999961
CGTCAAT301.13992246E-437.49999627
TATCGAG301.13992246E-437.4999961
Produced by FastQC (version 0.11.5)