##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1149931
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.872312338740326	33.0	31.0	34.0	30.0	34.0
2	31.98217371303148	33.0	31.0	34.0	30.0	34.0
3	31.91259823415492	33.0	31.0	34.0	30.0	34.0
4	35.66732351767193	37.0	35.0	37.0	33.0	37.0
5	31.47895917233295	37.0	35.0	37.0	0.0	37.0
6	33.19880671101136	37.0	35.0	37.0	17.0	37.0
7	35.149576800695	37.0	35.0	37.0	32.0	37.0
8	35.32871624471381	37.0	35.0	37.0	33.0	37.0
9	37.375841680935636	39.0	37.0	39.0	34.0	39.0
10	37.009150983841636	39.0	37.0	39.0	33.0	39.0
11	37.096130115633024	39.0	37.0	39.0	34.0	39.0
12	37.1242926749518	39.0	37.0	39.0	34.0	39.0
13	37.04865944130561	39.0	37.0	39.0	33.0	39.0
14	38.080251771628035	40.0	37.0	41.0	33.0	41.0
15	38.24405029519163	40.0	38.0	41.0	33.0	41.0
16	38.14021363020912	40.0	37.0	41.0	33.0	41.0
17	38.140856277463605	40.0	37.0	41.0	33.0	41.0
18	38.07414444866692	40.0	37.0	41.0	33.0	41.0
19	37.980948422122715	40.0	37.0	41.0	34.0	41.0
20	37.939378101816544	40.0	36.0	41.0	34.0	41.0
21	37.82887147141872	39.0	36.0	41.0	33.0	41.0
22	37.82686004638539	39.0	36.0	41.0	33.0	41.0
23	37.58703522211333	39.0	36.0	41.0	33.0	41.0
24	37.35508130487829	39.0	35.0	41.0	32.0	41.0
25	37.16443247464413	39.0	35.0	40.0	32.0	41.0
26	37.446773762947515	39.0	35.0	41.0	33.0	41.0
27	37.55322710666988	39.0	36.0	41.0	33.0	41.0
28	37.433504271125834	39.0	35.0	41.0	33.0	41.0
29	37.39096780589444	39.0	35.0	41.0	33.0	41.0
30	37.199302392926185	39.0	35.0	41.0	32.0	41.0
31	37.090210630029105	39.0	35.0	41.0	32.0	41.0
32	36.9139444018815	39.0	35.0	41.0	31.0	41.0
33	36.76215616415246	39.0	35.0	41.0	31.0	41.0
34	36.5314101454783	39.0	35.0	41.0	30.0	41.0
35	36.46304343478	39.0	35.0	41.0	30.0	41.0
36	36.30333733067462	39.0	35.0	41.0	30.0	41.0
37	36.267845635955545	39.0	35.0	41.0	30.0	41.0
38	36.1278554974168	39.0	35.0	41.0	29.0	41.0
39	36.05731039514545	39.0	35.0	41.0	29.0	41.0
40	35.980051846589056	39.0	35.0	40.0	29.0	41.0
41	35.82266153360506	39.0	35.0	40.0	28.0	41.0
42	35.860832519516386	39.0	35.0	40.0	28.0	41.0
43	35.76647120566364	39.0	35.0	40.0	28.0	41.0
44	35.71907966651912	39.0	35.0	40.0	28.0	41.0
45	35.64257855471328	39.0	35.0	40.0	27.0	41.0
46	35.526953356331816	38.0	35.0	40.0	27.0	41.0
47	35.448452124518774	38.0	35.0	40.0	27.0	41.0
48	35.398027359902464	38.0	35.0	40.0	26.0	41.0
49	35.35570829901968	38.0	35.0	40.0	26.0	41.0
50	35.22368211657917	38.0	35.0	40.0	26.0	41.0
51	34.09080805717908	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	13.0
10	33.0
11	23.0
12	21.0
13	30.0
14	41.0
15	41.0
16	102.0
17	204.0
18	412.0
19	807.0
20	1339.0
21	2296.0
22	3723.0
23	5378.0
24	7990.0
25	11813.0
26	14892.0
27	16228.0
28	16109.0
29	16662.0
30	19500.0
31	24721.0
32	32331.0
33	45194.0
34	68302.0
35	87783.0
36	111678.0
37	165771.0
38	244197.0
39	252203.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.21371717085634	20.220865425838593	23.02633810202525	16.539079301279816
2	36.185736361573	25.777720576278057	22.930071456461302	15.106471605687647
3	29.35750058046961	24.51712320130512	30.49861252544718	15.62676369277809
4	25.689367449003463	24.470163862005634	32.410901175809684	17.429567513181226
5	22.005581204437483	36.62915427099539	25.137682173973918	16.22758235059321
6	23.36253218671381	33.846552532282374	28.242564119064532	14.548351161939282
7	73.04099115512147	5.721560684945445	15.667722672055975	5.569725487877099
8	72.29425069852017	6.546305821827571	14.761320461836405	6.398123017815852
9	65.7277697531417	7.701157721637212	17.242338888159374	9.328733637061703
10	33.679412069071965	25.59814458432723	24.473294484625598	16.249148861975197
11	27.149628977738665	23.294788991687327	29.58325325606493	19.97232877450908
12	22.688491744287266	20.376005169005794	34.91957343527568	22.01592965143126
13	23.224089097519766	21.339106433342522	37.24319111320592	18.193613355931788
14	19.190629698651485	26.618727558436117	33.97551679187708	20.215125951035322
15	18.177351510655857	23.960481107127297	37.831835127498955	20.03033225471789
16	19.88728019333334	25.676410149826378	32.463252142954666	21.973057513885617
17	20.05372496262819	24.983412048201153	31.332575606710318	23.630287382460338
18	21.0425669018402	24.3201548614656	32.75066069181542	21.88661754487878
19	21.012652063471634	26.285055364191418	30.98855496547184	21.71373760686511
20	21.004477659963946	26.101653055705082	31.178827251374212	21.71504203295676
21	20.604279735044972	27.708792962360352	32.088099198995415	19.59882810359926
22	19.260459975424613	24.2593686055946	31.285094496974164	25.195076922006628
23	18.72877590046707	26.198354509966247	31.259701669056668	23.813167920510015
24	19.9325872595834	24.506252983874685	31.280659448262547	24.280500308279365
25	19.579348673963917	27.56156673748251	29.10461584216792	23.754468746385655
26	19.1268867436394	26.244009423174084	30.61027139889263	24.018832434293884
27	22.24768268704818	25.354738675624883	29.325933469051623	23.071645168275314
28	20.21756087973974	26.121393370558753	30.66505729474203	22.99598845495947
29	21.61538387955451	25.903641174992238	28.619630221291537	23.86134472416171
30	24.10457670938517	26.77482388073719	27.60087344371097	21.519725966166668
31	22.823543325643016	29.042612121944707	25.61388465916651	22.51995989324577
32	23.084602467452395	29.171402458060523	25.886422750582426	21.85757232390465
33	22.811281720381483	25.91485923938045	25.79667823547674	25.47718080476133
34	20.57488666711307	28.708244233784463	26.743082845840316	23.97378625326215
35	22.264118455803	26.77230199029333	27.22737277280115	23.736206781102517
36	21.04908903229846	28.42848831799473	26.47706688488266	24.04535576482415
37	22.14098063275101	28.22586746509138	27.663311972631398	21.969839929526206
38	20.730374257238044	26.588551834849223	27.71427155194529	24.966802355967445
39	22.778758029829614	24.739484369062144	26.69194934304754	25.789808258060702
40	21.75747936180519	24.466424507209563	30.50435200025045	23.2717441307348
41	19.503952845866404	26.241661456209115	28.280218552243568	25.974167145680916
42	21.32562736372878	24.876362146946207	30.094849169211024	23.70316132011399
43	22.39760472584877	23.324008136140343	29.32419423426275	24.954192903748137
44	22.01071194706465	24.389202482583737	27.32137841313957	26.27870715721204
45	21.79095963149093	23.154954514662187	28.196561358898926	26.85752449494796
46	23.29435418299011	24.384158701696016	27.690096188379997	24.63139092693388
47	20.004069809405955	24.313806654486225	31.094039555416803	24.588083980691017
48	20.038332734746696	23.772208941232126	30.39286705028389	25.796591273737295
49	21.946186336397574	21.670604584101135	31.34718517893682	25.03602390056447
50	20.560798865323225	21.079960449800904	31.73633896294647	26.622901721929402
51	20.53184060608854	21.388674624825317	28.95460684162789	29.124877927458254
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	822.0
1	1188.5
2	1555.0
3	11025.0
4	20495.0
5	13217.5
6	5940.0
7	5758.5
8	5577.0
9	5659.0
10	5741.0
11	5920.5
12	6100.0
13	6148.5
14	6197.0
15	6071.5
16	5946.0
17	5655.0
18	5364.0
19	5095.0
20	4826.0
21	4983.5
22	5141.0
23	5257.5
24	5374.0
25	6061.5
26	7955.0
27	9161.0
28	10472.5
29	11784.0
30	13253.0
31	14722.0
32	16495.5
33	18269.0
34	20168.0
35	22067.0
36	24007.0
37	25947.0
38	29524.5
39	33102.0
40	36752.0
41	40402.0
42	46531.5
43	52661.0
44	63981.5
45	75302.0
46	104227.0
47	133152.0
48	137998.0
49	142844.0
50	135313.0
51	127782.0
52	106423.0
53	85064.0
54	72120.0
55	59176.0
56	53073.5
57	46971.0
58	42709.5
59	38448.0
60	35142.0
61	31836.0
62	28842.0
63	25848.0
64	22675.0
65	19502.0
66	17093.5
67	14685.0
68	12825.5
69	10966.0
70	9693.5
71	8421.0
72	7499.5
73	6578.0
74	5269.0
75	3308.0
76	2656.0
77	2101.0
78	1546.0
79	1118.0
80	690.0
81	496.5
82	303.0
83	229.5
84	156.0
85	109.5
86	63.0
87	42.5
88	22.0
89	16.5
90	11.0
91	7.0
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1149931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.519983079343895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22283605767878	28.97582372122046
2	10.284478140479665	7.923158479023177
3	3.759889200814727	4.344926051867733
4	1.966215124790471	3.029542933491159
5	1.2717186528944808	2.4493290495590703
6	0.8988372604053975	2.077391763713984
7	0.7062463646788785	1.9043218612094972
8	0.572381383582048	1.7638496968409547
9	0.5058557191494373	1.7536998368003092
>10	4.678743960931766	33.71618247930455
>50	0.08079564282643364	2.128728009625969
>100	0.04341279454891263	3.0901041640815508
>500	0.003946617641162889	1.0961799043802587
>1k	0.004178771620054823	4.0864849967747325
>5k	2.3215397889193463E-4	0.527522047311074
>10k+	2.3215397889193463E-4	1.132755004795622
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12667	1.101544353530777	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	5899	0.512987300977189	Illumina PCR Primer Index 7 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	4576	0.39793691969344247	Illumina PCR Primer Index 7 (96% over 28bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	4521	0.3931540240240501	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	4129	0.35906502216219927	No Hit
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	3972	0.34541202906957025	Illumina PCR Primer Index 7 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	3806	0.33097638032194976	Illumina PCR Primer Index 7 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	3129	0.27210328271870227	Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	3033	0.26375495573212654	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	2497	0.21714346339041213	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	2485	0.21609992251709018	Illumina PCR Primer Index 7 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	2092	0.18192395891579582	Illumina PCR Primer Index 7 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	2079	0.18079345630303034	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1695	0.1474001483567275	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	1679	0.14600876052563155	Illumina PCR Primer Index 7 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	1325	0.1152243047626336	Illumina PCR Primer Index 7 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1278	0.1111371030087892	Illumina PCR Primer Index 7 (96% over 27bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC	1224	0.10644116907884038	Illumina PCR Primer Index 7 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	1160	0.10087561775445657	Illumina PCR Primer Index 7 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.696173944349704E-5	0.0	0.0	0.4221992449981781	0.0
2	8.696173944349704E-5	0.0	0.0	1.2793811106927284	0.0
3	1.739234788869941E-4	0.0	0.0	2.1122136893430996	0.0
4	1.739234788869941E-4	0.0	0.0	3.101316513773435	0.0
5	1.739234788869941E-4	0.0	0.0	5.353886450578339	0.0
6	1.739234788869941E-4	0.0	0.0	7.247478326960487	0.0
7	1.739234788869941E-4	0.0	0.0	8.858009741454053	0.0
8	1.739234788869941E-4	0.0	0.0	11.281546458004872	0.0
9	1.739234788869941E-4	0.0	0.0	12.345001569659397	0.0
10	1.739234788869941E-4	0.0	0.0	14.5140012748591	0.0
11	1.739234788869941E-4	0.0	0.0	16.705176223616895	0.0
12	1.739234788869941E-4	0.0	0.0	18.895655478459144	0.0
13	1.739234788869941E-4	0.0	0.0	19.748576218920963	0.0
14	1.739234788869941E-4	0.0	0.0	20.158513858657606	0.0
15	1.739234788869941E-4	0.0	0.0	20.74976672513394	0.0
16	1.739234788869941E-4	0.0	0.0	21.70434573900521	0.0
17	1.739234788869941E-4	0.0	0.0	22.83284823176347	0.0
18	1.739234788869941E-4	0.0	0.0	24.146231382578605	0.0
19	1.739234788869941E-4	0.0	0.0	24.999847816955974	0.0
20	1.739234788869941E-4	0.0	0.0	25.809548572914373	0.0
21	1.739234788869941E-4	0.0	0.0	26.664556395122837	0.0
22	1.739234788869941E-4	0.0	0.0	27.563827742708042	0.0
23	1.739234788869941E-4	0.0	0.0	28.41779202404318	0.0
24	1.739234788869941E-4	0.0	0.0	29.109398737837314	0.0
25	1.739234788869941E-4	0.0	0.0	29.725609623533934	0.0
26	1.739234788869941E-4	0.0	0.0	30.295035093409954	0.0
27	1.739234788869941E-4	0.0	0.0	30.888375041632933	0.0
28	1.739234788869941E-4	0.0	0.0	31.466844532411073	0.0
29	2.6088521833049114E-4	0.0	0.0	32.0716634302406	0.0
30	2.6088521833049114E-4	0.0	0.0	32.78761943107891	0.0
31	2.6088521833049114E-4	0.0	0.0	33.384090001921855	0.0
32	2.6088521833049114E-4	0.0	0.0	33.98082145798313	0.0
33	2.6088521833049114E-4	0.0	0.0	34.57990088100938	0.0
34	2.6088521833049114E-4	0.0	0.0	35.16576211964022	0.0
35	2.6088521833049114E-4	0.0	0.0	35.72327383121248	0.0
36	2.6088521833049114E-4	0.0	0.0	36.255914485303904	0.0
37	3.478469577739882E-4	0.0	0.0	36.79133791505751	0.0
38	3.478469577739882E-4	0.0	0.0	37.344762424875924	0.0
39	4.348086972174852E-4	0.0	0.0	38.00193228985044	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	25	3.8908078E-5	45.0	1
CGATAAC	25	3.8908078E-5	45.0	10
CGTTATT	885	0.0	43.98305	1
CGTTTTT	6045	0.0	43.36228	1
TAATACG	115	0.0	43.04348	4
CGTTTCT	370	0.0	41.959457	1
TTACGGG	325	0.0	40.153847	3
AGTTACG	45	1.9283107E-8	40.0	1
TACGGGA	485	0.0	39.896908	4
TCGTACG	40	3.4582263E-7	39.375	30
CGGTAAT	40	3.4582263E-7	39.375	6
GACGGGA	505	0.0	39.207916	4
TGTTGCG	35	6.2482904E-6	38.57143	1
CGTTCGG	135	0.0	38.333336	45
ACTACGG	65	9.094947E-12	38.07692	2
TCGACGG	65	9.094947E-12	38.07692	2
CATACGA	95	0.0	37.894737	18
ACGTATG	30	1.13992246E-4	37.499996	1
CGTCAAT	30	1.13992246E-4	37.499996	27
TATCGAG	30	1.13992246E-4	37.499996	1
>>END_MODULE