FastQCFastQC Report
Sat 18 Jun 2016
SRR3550022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330735
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC104280.7836270932980646No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG89320.6712080166223929No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC79250.5955355499028733No Hit
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC78150.5872694413237797No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT67410.5065621630151759TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC51010.3833219987450544No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.23828936640277742No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCC29470.2214565634780779No Hit
TCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC27150.20402258902035342No Hit
ACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC21410.16088853152581092TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCT20640.15510225552044546No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTA18450.13864518480388657No Hit
CGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG17860.13421154474782732TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTC16740.12579514328547756No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT16300.12248869985384016No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGACTTT15230.11444803059963103No Hit
AGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG14520.10911263324403431TruSeq Adapter, Index 13 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG13610.10227430705587513TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA253.8911392E-545.032
GTATTCG207.033688E-445.01
AGCGCAT207.033688E-445.023
TTCGCGA207.033688E-445.014
CCCGATA207.033688E-445.045
CGTTAGG1200.044.9999962
CGTTTTT23950.041.8058471
TCGACAC700.041.78571734
TAGAACG556.184564E-1140.9090921
TATGCGA501.0822987E-940.51
ACACGCG403.458772E-739.37536
GACGTAG403.458772E-739.3751
ATTCGAC403.458772E-739.37528
TATAGCG403.458772E-739.3751
CTAACGG750.039.0000042
GTACGAG750.039.0000041
TCACGAC700.038.5714325
GTCGATG356.249031E-638.571431
CGTTCGG1000.038.2545
TTTACGG950.037.8947372
Produced by FastQC (version 0.11.5)