FastQCFastQC Report
Sat 18 Jun 2016
SRR3550021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435801
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT36340.8338668337153885Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC34240.7856797024329911Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC29460.6759966131330585Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC10390.23841156858290824Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC9560.21936617859986554Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC8460.19412530030908604No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7700.17668614803545657No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG7650.17553883538587567No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.1551166702233359No Hit
GCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG6380.14639709408652116No Hit
AGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG6000.13767751794970642No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG5010.11496072748800484No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC4860.1115187895392622No Hit
GGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG4770.1094536267700166No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC207.0284604E-445.00000437
GTACAAG406.7975634E-945.0000041
TTTCGCG207.0284604E-445.0000041
GTTAACG207.0284604E-445.0000041
CCATACG207.0284604E-445.0000041
ACTACCG207.0284604E-445.0000041
ACTAATG207.0284604E-445.00000417
CATAACT207.0284604E-445.00000428
CGATGAC207.0284604E-445.00000410
GTTATCA207.0284604E-445.00000440
AAATGCG207.0284604E-445.0000041
CGGGTCA253.886807E-545.06
CGGGTAC253.886807E-545.06
GATCGAG253.886807E-545.01
TACCAGG351.2096098E-745.02
CGCATGG453.8380676E-1045.02
CCAGCGG253.886807E-545.02
TATGCGG253.886807E-545.02
GTAATCG253.886807E-545.01
TCGTTTA302.1623891E-644.99999638