##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	435801
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29661703392144	33.0	31.0	34.0	30.0	34.0
2	32.461777279079215	34.0	31.0	34.0	31.0	34.0
3	32.46211229437289	34.0	31.0	34.0	31.0	34.0
4	36.01650753440217	37.0	35.0	37.0	35.0	37.0
5	35.89832515299414	37.0	35.0	37.0	35.0	37.0
6	35.84951847288097	37.0	35.0	37.0	35.0	37.0
7	36.048590985335046	37.0	35.0	37.0	35.0	37.0
8	36.01043366123529	37.0	35.0	37.0	35.0	37.0
9	37.572155639844794	39.0	38.0	39.0	35.0	39.0
10	37.40383799027538	39.0	37.0	39.0	35.0	39.0
11	37.32188774234111	39.0	37.0	39.0	34.0	39.0
12	37.16772793086753	39.0	37.0	39.0	34.0	39.0
13	37.0479140708718	39.0	37.0	39.0	33.0	39.0
14	38.1699996099137	40.0	37.0	41.0	33.0	41.0
15	38.280212757657736	40.0	37.0	41.0	34.0	41.0
16	38.3118120426525	40.0	37.0	41.0	34.0	41.0
17	38.3200107388464	40.0	37.0	41.0	34.0	41.0
18	38.29532515987802	40.0	37.0	41.0	34.0	41.0
19	38.22213120208536	40.0	37.0	41.0	34.0	41.0
20	38.169676067746515	40.0	37.0	41.0	34.0	41.0
21	38.18455441818628	40.0	37.0	41.0	34.0	41.0
22	38.07592226727336	40.0	36.0	41.0	34.0	41.0
23	37.824741108900625	40.0	36.0	41.0	33.0	41.0
24	37.66963132255319	39.0	36.0	41.0	33.0	41.0
25	37.6564326378324	39.0	35.0	41.0	33.0	41.0
26	37.73198317580731	40.0	36.0	41.0	33.0	41.0
27	37.797361639831024	40.0	36.0	41.0	33.0	41.0
28	37.784130830356055	40.0	36.0	41.0	33.0	41.0
29	37.767077175132684	40.0	36.0	41.0	33.0	41.0
30	37.67826140830333	40.0	36.0	41.0	33.0	41.0
31	37.495902946528346	39.0	35.0	41.0	33.0	41.0
32	37.3999486003933	39.0	35.0	41.0	33.0	41.0
33	37.2866216461183	39.0	35.0	41.0	33.0	41.0
34	37.17094958478755	39.0	35.0	41.0	33.0	41.0
35	37.1508371940404	39.0	35.0	41.0	33.0	41.0
36	37.01293709743668	39.0	35.0	41.0	32.0	41.0
37	36.86617745255288	39.0	35.0	41.0	31.0	41.0
38	36.7736030894835	39.0	35.0	41.0	31.0	41.0
39	36.74656093033288	39.0	35.0	41.0	31.0	41.0
40	36.67756154758709	39.0	35.0	41.0	31.0	41.0
41	36.64266488603744	39.0	35.0	41.0	31.0	41.0
42	36.602277186146885	39.0	35.0	41.0	31.0	41.0
43	36.486979148739906	39.0	35.0	41.0	31.0	41.0
44	36.36585735232365	39.0	35.0	40.0	31.0	41.0
45	36.3391731547197	39.0	35.0	40.0	31.0	41.0
46	36.266266025089436	38.0	35.0	40.0	31.0	41.0
47	36.165777499363244	38.0	35.0	40.0	30.0	41.0
48	36.12714748245185	38.0	35.0	40.0	30.0	41.0
49	36.07330639443232	38.0	35.0	40.0	30.0	41.0
50	35.953751827095395	38.0	35.0	40.0	30.0	41.0
51	34.956548975335075	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	2.0
14	12.0
15	12.0
16	25.0
17	62.0
18	117.0
19	223.0
20	375.0
21	598.0
22	937.0
23	1288.0
24	1835.0
25	2363.0
26	2836.0
27	3342.0
28	3884.0
29	4701.0
30	5875.0
31	7453.0
32	10358.0
33	14759.0
34	28132.0
35	38976.0
36	33795.0
37	53460.0
38	93924.0
39	126359.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.085263686866256	21.419179855025575	23.95726489842841	12.538291559679763
2	31.62613211075697	24.301229230772762	25.91343296596382	18.15920569250644
3	30.667208198237265	25.12752380100092	27.018983435099962	17.18628456566185
4	29.24201642492789	25.76887157211663	26.608474969079925	18.38063703387555
5	25.03046115084637	29.45518711522002	25.394618185823347	20.11973354811026
6	25.528853765824312	33.06715679863057	25.894158113450867	15.509831322094259
7	76.43809904061716	7.06216828323019	10.161289212278081	6.338443463874567
8	75.39817485503704	6.265703841891139	10.77303631703461	7.563084986037206
9	69.25064421605273	7.966939038689677	13.9742680718952	8.808148673362384
10	38.071046188512646	24.922154836725937	21.045155931262205	15.961643043499214
11	28.547892271931456	25.106642710778544	25.28034584592509	21.065119171364913
12	26.219535980872006	21.28264964972545	31.99005968320403	20.507754686198517
13	23.202562637534104	26.06717286100766	31.61190543390217	19.118359067556064
14	17.461410139031347	30.270696946542113	29.12453160961081	23.14336130481573
15	16.57339014825574	25.901959839468013	35.96962833954029	21.55502167273595
16	17.840941163512706	25.224815913685372	32.87463773603089	24.059605186771027
17	17.53644438631394	25.94555772015209	28.676391288684517	27.84160660484946
18	19.313861143044647	25.344824816831533	31.816356548057485	23.524957492066335
19	21.20692701485311	27.886351798183117	27.752116218182156	23.154604968781623
20	23.411373539757825	25.81843547857853	28.677538601334096	22.092652380329554
21	21.588293739573796	27.102737258519372	28.9712506396268	22.337718362280032
22	19.390042702976817	26.215635117863428	26.211045867265103	28.18327631189465
23	19.7812763164839	26.705537619234466	27.392548433803505	26.120637630478132
24	22.53276151270878	23.985259327078186	28.930176846771804	24.551802313441225
25	20.108260421614453	26.98158104272363	27.200029371203826	25.71012916445809
26	19.862047127014396	27.16813407954548	28.385203338220887	24.58461545521924
27	21.59586600306103	26.63715778531945	28.477676737777102	23.28929947384242
28	19.373980325882684	24.909534397580547	31.173632001762275	24.542853274774494
29	20.883614310201214	23.061902106695488	30.96619787471805	25.08828570838525
30	23.503388014254213	25.5726811090383	29.035041222943498	21.88888965376399
31	23.046528117191105	28.31361102888704	26.611228519438917	22.02863233448294
32	26.31223884295814	26.495579404361163	25.510496763431018	21.681684989249682
33	25.700721200731525	25.787916962099672	26.927886810723244	21.583475026445555
34	21.396921989623703	25.01761124917107	30.3328812921494	23.25258546905583
35	25.433397353379178	22.85423851712135	28.045139868885112	23.667224260614365
36	26.20416199136762	24.926514624794343	28.77161823859973	20.097705145238308
37	24.76405515361369	26.86455515246638	29.74270366520499	18.62868602871494
38	24.561669202227623	25.524264515225987	28.22159655439065	21.692469728155743
39	24.88383459422994	24.437988898602804	29.319574760039558	21.358601747127704
40	24.966670567529675	23.75556733463209	30.524482504629407	20.753279593208827
41	22.538498075956685	23.981587926599527	27.52655455127455	25.953359446169237
42	25.340694491293043	23.393475462424362	27.87281350891806	23.39301653736453
43	26.80856629516683	22.046071486756567	28.987313016720933	22.158049201355663
44	22.58255488170059	23.00706056204552	29.123613759491146	25.28677079676274
45	22.6975156091886	22.733541226385437	28.88198971548941	25.686953448936556
46	24.34941636205516	23.542167181810047	29.861794718231486	22.24662173790331
47	21.387514025897143	23.520138778938094	32.34733284228352	22.745014352881245
48	22.901278335754164	21.147266757074902	30.609154178168474	25.34230072900246
49	23.868003974291017	20.588754959258928	32.6775294228329	22.865711643617157
50	21.59770170330036	21.20853325256252	32.144258503307704	25.049506540829412
51	20.84139320469664	21.0167025775526	28.84825872359173	29.293645494159033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	571.0
1	748.0
2	925.0
3	1228.0
4	1531.0
5	1155.0
6	779.0
7	862.5
8	946.0
9	966.0
10	986.0
11	1073.0
12	1160.0
13	1211.5
14	1263.0
15	1204.0
16	1145.0
17	1171.5
18	1198.0
19	1180.5
20	1163.0
21	1339.0
22	1515.0
23	1566.0
24	1617.0
25	2053.0
26	2810.0
27	3131.0
28	3840.5
29	4550.0
30	4839.0
31	5128.0
32	6023.5
33	6919.0
34	8916.5
35	10914.0
36	11634.5
37	12355.0
38	12617.5
39	12880.0
40	14929.5
41	16979.0
42	20466.0
43	23953.0
44	28002.0
45	32051.0
46	37068.0
47	42085.0
48	48691.5
49	55298.0
50	52605.5
51	49913.0
52	41066.5
53	32220.0
54	27180.0
55	22140.0
56	19241.0
57	16342.0
58	16375.0
59	16408.0
60	15391.5
61	14375.0
62	13075.0
63	11775.0
64	10649.5
65	9524.0
66	8027.5
67	6531.0
68	5549.5
69	4568.0
70	3715.0
71	2862.0
72	2523.0
73	2184.0
74	1908.5
75	1278.0
76	923.0
77	720.0
78	517.0
79	352.0
80	187.0
81	141.0
82	95.0
83	62.0
84	29.0
85	27.5
86	26.0
87	20.5
88	15.0
89	9.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	435801.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.396543933357854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.66425282938565	23.216741152384575
2	11.906762069771991	7.714758809948146
3	4.195834319090046	4.07791592566474
4	2.0880354558996865	2.705805295258524
5	1.221685814960311	1.978919908856091
6	0.8519744743860417	1.6560617093728123
7	0.6114444800330028	1.3866081572138829
8	0.518768821553931	1.3445053534982572
9	0.4492601820394039	1.3099029502453108
>10	6.291700973819634	44.383759763363514
>50	0.13161295195391345	2.725590860022017
>100	0.060084173718090925	3.6075496096601567
>500	0.005722302258865802	1.332902450845659
>1k	0.002861151129432901	2.55897805366631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	3634	0.8338668337153885	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	3424	0.7856797024329911	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	2946	0.6759966131330585	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1039	0.23841156858290824	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	956	0.21936617859986554	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC	846	0.19412530030908604	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	770	0.17668614803545657	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	765	0.17553883538587567	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	676	0.1551166702233359	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	638	0.14639709408652116	No Hit
AGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	600	0.13767751794970642	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	501	0.11496072748800484	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	486	0.1115187895392622	No Hit
GGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTG	477	0.1094536267700166	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8758584766900489	0.0
2	0.0	0.0	0.0	2.8976528277814873	0.0
3	0.0	0.0	0.0	3.9770445685071856	0.0
4	0.0	0.0	0.0	5.027524030463446	0.0
5	0.0	0.0	0.0	7.465792873352746	0.0
6	0.0	0.0	0.0	8.805624585533305	0.0
7	0.0	0.0	0.0	10.160600824688332	0.0
8	0.0	0.0	0.0	12.299879991096853	0.0
9	0.0	0.0	0.0	13.00065855746086	0.0
10	0.0	0.0	0.0	14.513505017198217	0.0
11	0.0	0.0	0.0	16.625707605076627	0.0
12	0.0	0.0	0.0	18.26911824433629	0.0
13	0.0	0.0	0.0	18.910007090392174	0.0
14	0.0	0.0	0.0	19.16356318594955	0.0
15	0.0	0.0	0.0	19.681919041030195	0.0
16	0.0	0.0	0.0	20.741347541653187	0.0
17	0.0	0.0	0.0	22.040564386038582	0.0
18	0.0	0.0	0.0	23.628904018118362	0.0
19	0.0	0.0	0.0	24.42192652150867	0.0
20	0.0	0.0	0.0	25.183742120830377	0.0
21	0.0	0.0	0.0	26.057994359811016	0.0
22	0.0	0.0	0.0	26.955422314313186	0.0
23	0.0	0.0	0.0	27.862028770012	0.0
24	0.0	0.0	0.0	28.613977480547312	0.0
25	0.0	0.0	0.0	29.204155107491722	0.0
26	0.0	0.0	0.0	29.765879380726524	0.0
27	2.2946252991617734E-4	0.0	0.0	30.34228925587596	0.0
28	2.2946252991617734E-4	0.0	0.0	30.916633968256154	0.0
29	2.2946252991617734E-4	0.0	0.0	31.514613321217713	0.0
30	2.2946252991617734E-4	0.0	0.0	32.228012326727104	0.0
31	2.2946252991617734E-4	0.0	0.0	32.80189811404747	0.0
32	2.2946252991617734E-4	0.0	0.0	33.31497633094004	0.0
33	2.2946252991617734E-4	0.0	0.0	33.84755886287549	0.0
34	2.2946252991617734E-4	0.0	0.0	34.39643323443498	0.0
35	2.2946252991617734E-4	0.0	0.0	34.995100974986286	0.0
36	2.2946252991617734E-4	0.0	0.0	35.52171748114392	0.0
37	2.2946252991617734E-4	0.0	0.0	36.063478514276014	0.0
38	2.2946252991617734E-4	0.0	0.0	36.58642361995498	0.0
39	2.2946252991617734E-4	0.0	0.0	37.121989164779336	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	20	7.0284604E-4	45.000004	37
GTACAAG	40	6.7975634E-9	45.000004	1
TTTCGCG	20	7.0284604E-4	45.000004	1
GTTAACG	20	7.0284604E-4	45.000004	1
CCATACG	20	7.0284604E-4	45.000004	1
ACTACCG	20	7.0284604E-4	45.000004	1
ACTAATG	20	7.0284604E-4	45.000004	17
CATAACT	20	7.0284604E-4	45.000004	28
CGATGAC	20	7.0284604E-4	45.000004	10
GTTATCA	20	7.0284604E-4	45.000004	40
AAATGCG	20	7.0284604E-4	45.000004	1
CGGGTCA	25	3.886807E-5	45.0	6
CGGGTAC	25	3.886807E-5	45.0	6
GATCGAG	25	3.886807E-5	45.0	1
TACCAGG	35	1.2096098E-7	45.0	2
CGCATGG	45	3.8380676E-10	45.0	2
CCAGCGG	25	3.886807E-5	45.0	2
TATGCGG	25	3.886807E-5	45.0	2
GTAATCG	25	3.886807E-5	45.0	1
TCGTTTA	30	2.1623891E-6	44.999996	38
>>END_MODULE