FastQCFastQC Report
Sat 18 Jun 2016
SRR3550020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657086
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC357232.1557722411510327No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC353992.136219846163687No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG351972.124029772745651No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158580.9569811102139539No Hit
GCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC84280.508603657263413No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT77660.46865401071519525No Hit
GAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT77080.46515389062486795No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC61140.36896093503897803No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTA61080.3685988536503235No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAAATGGT54230.3272612284455967No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT46520.28073377000348804Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG44560.26890577797410636No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT43450.2622072722839973No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC42290.25520703210334283No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC40170.24241348970421572No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC32580.19661019403941618No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT31090.18761850622116172No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT29120.17573016729367094No Hit
CGTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT19560.11803853270138062No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC17020.10271042058167168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGC351.2123564E-745.043
TACGGCT40600.042.506167
ATTAGCG900.042.51
CGTTTTT73050.041.889121
TGATACC40800.041.470594
CGATGAA11850.041.2025319
ACGGCTG42500.040.7117658
GATACCT41900.040.435565
CACAACG4250.039.70588312
CCGATAT403.4594632E-739.37511
CGGTCGA1150.039.13043641
GAATGAT144600.039.0404551
CCGATGA12250.038.755118
GATGAAT12850.038.696520
ATGATAC141150.038.5122223
GAATCTG47450.038.503691
GAATGAC10700.038.2710271
AATGATA143450.037.9888462
CGTTCGG2550.037.94117745
GTCGAAT1250.037.843
Produced by FastQC (version 0.11.5)