##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550020_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1657086
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17529084187544	33.0	31.0	34.0	30.0	34.0
2	32.351547234120616	34.0	31.0	34.0	30.0	34.0
3	32.31828824816576	34.0	31.0	34.0	30.0	34.0
4	35.90979224976857	37.0	35.0	37.0	35.0	37.0
5	35.786560866484905	37.0	35.0	37.0	35.0	37.0
6	35.69604534707312	37.0	35.0	37.0	33.0	37.0
7	35.88683689319685	37.0	35.0	37.0	35.0	37.0
8	35.65985410533913	37.0	35.0	37.0	35.0	37.0
9	37.38388894722422	39.0	37.0	39.0	34.0	39.0
10	37.27276375517022	39.0	37.0	39.0	34.0	39.0
11	37.14704668315344	39.0	37.0	39.0	34.0	39.0
12	36.99686196129833	39.0	37.0	39.0	33.0	39.0
13	36.85304806147659	39.0	37.0	39.0	33.0	39.0
14	37.919205158935625	40.0	37.0	41.0	33.0	41.0
15	37.931433250899474	40.0	37.0	41.0	33.0	41.0
16	38.09091501587727	40.0	37.0	41.0	33.0	41.0
17	38.006752214429426	40.0	37.0	41.0	33.0	41.0
18	37.95629375904449	40.0	37.0	41.0	33.0	41.0
19	37.84153508025534	40.0	37.0	41.0	33.0	41.0
20	37.66004118072327	40.0	35.0	41.0	33.0	41.0
21	37.76062678702252	40.0	36.0	41.0	33.0	41.0
22	37.71324662691013	40.0	35.0	41.0	33.0	41.0
23	37.43142842314762	39.0	35.0	41.0	33.0	41.0
24	37.22188528537445	39.0	35.0	41.0	32.0	41.0
25	37.23544281950363	39.0	35.0	41.0	32.0	41.0
26	37.306898374616644	39.0	35.0	41.0	32.0	41.0
27	37.34322600034036	39.0	35.0	41.0	33.0	41.0
28	37.29452605356632	39.0	35.0	41.0	33.0	41.0
29	37.245039786709924	39.0	35.0	41.0	32.0	41.0
30	37.161237256243794	39.0	35.0	41.0	32.0	41.0
31	36.9083807358218	39.0	35.0	41.0	31.0	41.0
32	36.65699004155488	39.0	35.0	41.0	31.0	41.0
33	36.512062741463026	39.0	35.0	41.0	30.0	41.0
34	36.34229424423355	39.0	35.0	41.0	30.0	41.0
35	36.28764650718188	39.0	35.0	41.0	30.0	41.0
36	36.123916924046185	39.0	35.0	41.0	30.0	41.0
37	35.96436093238371	39.0	35.0	40.0	30.0	41.0
38	35.73690442137584	38.0	35.0	40.0	28.0	41.0
39	35.73380560815794	38.0	35.0	40.0	28.0	41.0
40	35.62044697740492	38.0	35.0	40.0	27.0	41.0
41	35.53437781744581	38.0	35.0	40.0	27.0	41.0
42	35.507283267132784	38.0	35.0	40.0	27.0	41.0
43	35.473909018602534	38.0	35.0	40.0	27.0	41.0
44	35.38142317296749	38.0	35.0	40.0	27.0	41.0
45	35.30772090283787	38.0	35.0	40.0	27.0	41.0
46	35.276533022426115	38.0	34.0	40.0	27.0	41.0
47	35.01795621953236	37.0	34.0	40.0	26.0	41.0
48	34.97470982194044	37.0	34.0	40.0	26.0	41.0
49	34.97153738550685	37.0	34.0	40.0	26.0	41.0
50	34.83372800204697	37.0	34.0	40.0	26.0	41.0
51	33.802412186211214	36.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	1.0
10	7.0
11	12.0
12	11.0
13	21.0
14	43.0
15	71.0
16	123.0
17	266.0
18	611.0
19	1311.0
20	2437.0
21	4134.0
22	6067.0
23	8093.0
24	10942.0
25	14586.0
26	18747.0
27	21369.0
28	23183.0
29	25580.0
30	30368.0
31	38443.0
32	49776.0
33	68339.0
34	114609.0
35	132113.0
36	140733.0
37	205882.0
38	328275.0
39	410656.0
40	273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.0495882531142	17.766609578501054	19.774471572386705	14.409330595998036
2	32.01046897988397	31.919526204433566	20.521747211671574	15.548257604010896
3	27.830299694765387	31.648991060210513	26.153078355619442	14.367630889404653
4	24.802454429039894	21.617526187536434	36.59719531756348	16.982824065860193
5	30.469571283566456	24.942157498162437	25.14709556414091	19.441175654130202
6	24.024220831025065	36.72507039465665	25.81133387162766	13.439374902690627
7	70.37697500310787	4.966489367480023	19.51546268570249	5.14107294370962
8	68.53760154874278	11.505739593479156	13.938504096950913	6.018154760827139
9	63.32266400174764	5.753292225026342	14.719755039871195	16.204288733354815
10	41.14167882656663	20.21017617673434	22.1351215326181	16.513023464080923
11	31.608860372967968	21.124974805170037	27.28500512345165	19.98115969841034
12	25.548342089668246	19.119345646514425	31.546763414813718	23.78554884900361
13	24.313342819865717	20.2962308534379	37.228665259377	18.16176106731938
14	20.282894188955794	26.975063454763358	31.079014607570155	21.66302774871069
15	17.668304481481346	22.0547998112349	39.211966065732256	21.064929641551494
16	20.75480693216888	24.197537122394372	29.551332881938535	25.49632306349821
17	21.03336821384044	24.501263060577426	31.941070047058513	22.524298678523625
18	21.675941984906032	23.324800281940707	31.106351752413573	23.892905980739684
19	20.920278126784005	25.50863383071247	28.627844300175127	24.9432437423284
20	22.60359450263897	25.929855179513915	32.04595295597211	19.420597361875004
21	21.128957700445238	29.3121177778341	30.558341570684927	19.00058295103573
22	20.09853441523252	21.77497124470305	31.939802762198223	26.186691577866206
23	20.79982571815826	27.128103188368012	30.878723252746088	21.19334784072764
24	22.361120666036648	23.703778802065795	28.259245446524805	25.675855085372756
25	21.21380543918662	30.285332203639403	26.167561611165624	22.333300746008355
26	21.221952270431345	22.756392848651185	29.65893140126704	26.362723479650423
27	24.666070439313348	24.46312382097248	27.09455031301936	23.77625542669481
28	19.923830145206704	25.208770094008397	30.626473218650084	24.240926542134808
29	26.716476996365913	23.459615252316414	26.354697342201916	23.469210409115764
30	25.398621435459596	26.868068404415947	27.92232871438175	19.810981445742705
31	25.31323057463523	26.786479398172453	25.058989092901633	22.841300934290675
32	27.556204083553904	29.652232895576937	23.61518955564165	19.176373465227513
33	24.454252826950444	26.96987362152598	24.144431852058375	24.431441699465207
34	26.121637621704608	25.136715897666146	28.72439933714967	20.017247143479576
35	28.155086700388516	25.157052802328906	25.073049920161054	21.614810577121528
36	29.089739458302105	27.995227767297532	24.09235247899023	18.822680295410134
37	25.475322342956247	27.39761243532321	27.998969274980297	19.128095946740242
38	23.50318571275118	30.239106479687837	24.110516895321062	22.14719091223992
39	24.79816979927415	26.455838743432746	27.9418811093691	20.804110347924006
40	25.9662443590737	24.394388704026223	29.28647034613774	20.352896590762338
41	24.948071494177128	24.43910575552506	28.02141832107688	22.591404429220933
42	26.015728815523154	22.669312274679772	30.325402543983838	20.989556365813243
43	27.468037265416523	22.68645079374275	26.591257182789548	23.254254758051182
44	24.561066836603533	22.41265691702181	28.648905367615203	24.37737087875946
45	22.582714475893226	21.3796387151904	28.196907100778112	27.840739708138262
46	27.222123655621978	24.18836439388179	26.394767682546348	22.194744267949883
47	20.670864396899134	23.244659601251836	34.68691425792023	21.3975617439288
48	23.15697555829933	24.73287445552011	27.802902203023862	24.3072477831567
49	23.024996892134748	21.644742638583633	32.49602012206971	22.834240347211914
50	23.02813493083642	21.561041490906327	30.356058768223253	25.054764810033998
51	22.723202054691185	21.187071763324294	27.39918145467405	28.690544727310474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	948.0
1	1796.0
2	2644.0
3	13283.0
4	23922.0
5	15253.0
6	6584.0
7	6454.0
8	6324.0
9	6246.0
10	6168.0
11	6060.5
12	5953.0
13	5865.0
14	5777.0
15	5676.0
16	5575.0
17	5345.5
18	5116.0
19	4951.5
20	4787.0
21	4989.5
22	5192.0
23	5670.5
24	6149.0
25	7143.0
26	9606.0
27	11075.0
28	13050.0
29	15025.0
30	18156.0
31	21287.0
32	23487.0
33	25687.0
34	28136.0
35	30585.0
36	34317.0
37	38049.0
38	40302.0
39	42555.0
40	47102.5
41	51650.0
42	58629.5
43	65609.0
44	75709.5
45	85810.0
46	172698.0
47	259586.0
48	210611.0
49	161636.0
50	158043.0
51	154450.0
52	133162.5
53	111875.0
54	101026.0
55	90177.0
56	83782.5
57	77388.0
58	74211.0
59	71034.0
60	65355.5
61	59677.0
62	55667.5
63	51658.0
64	46448.5
65	41239.0
66	35639.0
67	30039.0
68	26733.0
69	23427.0
70	19564.0
71	15701.0
72	13542.0
73	11383.0
74	9495.5
75	5965.0
76	4322.0
77	3551.0
78	2780.0
79	2050.5
80	1321.0
81	932.5
82	544.0
83	476.0
84	408.0
85	272.0
86	136.0
87	90.0
88	44.0
89	33.5
90	23.0
91	16.5
92	10.0
93	7.5
94	5.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1657086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.306962846565888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62003114414891	22.311995486487447
2	9.685089720579771	5.870513086545367
3	3.4137929712704613	3.1038509023848495
4	1.8143480682489985	2.1994951798064393
5	1.2231735071202476	1.8535337017598523
6	0.943529611947192	1.7157310136350967
7	0.7807670135522056	1.656387381008563
8	0.6888805404318341	1.6702301555671861
9	0.6163248466436597	1.6811040805780335
>10	7.08257194168674	40.49865434344483
>50	0.08178199310717853	1.682779778398708
>100	0.04105491820455071	2.4414731875034583
>500	0.0030187406017097183	0.5942506371764591
>1k	0.003622488722051662	2.734053070108997
>5k	0.0012074962406838873	2.534058423767869
>10k+	8.049974937892582E-4	7.451889571826774
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC	35723	2.1557722411510327	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC	35399	2.136219846163687	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	35197	2.124029772745651	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15858	0.9569811102139539	No Hit
GCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	8428	0.508603657263413	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	7766	0.46865401071519525	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	7708	0.46515389062486795	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	6114	0.36896093503897803	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTA	6108	0.3685988536503235	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAAATGGT	5423	0.3272612284455967	No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	4652	0.28073377000348804	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	4456	0.26890577797410636	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT	4345	0.2622072722839973	No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	4229	0.25520703210334283	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC	4017	0.24241348970421572	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCC	3258	0.19661019403941618	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTT	3109	0.18761850622116172	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	2912	0.17573016729367094	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	1956	0.11803853270138062	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	1702	0.10271042058167168	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.034689810909029E-5	0.0	0.0	0.2988378394362151	0.0
2	6.034689810909029E-5	0.0	0.0	1.2340337194327875	0.0
3	6.034689810909029E-5	0.0	0.0	1.8359940280709632	0.0
4	6.034689810909029E-5	0.0	0.0	2.829726399233353	0.0
5	6.034689810909029E-5	0.0	0.0	6.375529091429171	0.0
6	6.034689810909029E-5	0.0	0.0	7.707445479594903	0.0
7	6.034689810909029E-5	0.0	0.0	9.164159253050235	0.0
8	6.034689810909029E-5	0.0	0.0	10.827862887019744	0.0
9	1.2069379621818059E-4	0.0	0.0	11.448772121664174	0.0
10	1.2069379621818059E-4	0.0	0.0	14.667554973006833	0.0
11	1.2069379621818059E-4	0.0	0.0	16.103449066614527	0.0
12	1.2069379621818059E-4	0.0	0.0	19.399717335129257	0.0
13	1.2069379621818059E-4	0.0	0.0	19.960822793747578	0.0
14	1.2069379621818059E-4	0.0	0.0	20.295989465845466	0.0
15	1.8104069432727088E-4	0.0	0.0	21.04000637263244	0.0
16	1.8104069432727088E-4	0.0	0.0	21.740211431392215	0.0
17	1.8104069432727088E-4	0.0	0.0	22.462744842452352	0.0
18	1.8104069432727088E-4	0.0	0.0	23.181114317542963	0.0
19	1.8104069432727088E-4	0.0	0.0	24.067067128682517	0.0
20	1.8104069432727088E-4	0.0	0.0	24.626362180357567	0.0
21	1.8104069432727088E-4	0.0	0.0	25.22458098131298	0.0
22	1.8104069432727088E-4	0.0	0.0	25.893586693750354	0.0
23	1.8104069432727088E-4	0.0	0.0	26.50278863016162	0.0
24	1.8104069432727088E-4	0.0	0.0	26.99962464229376	0.0
25	1.8104069432727088E-4	0.0	0.0	27.453372969176012	0.0
26	1.8104069432727088E-4	0.0	0.0	27.87658576561506	0.0
27	1.8104069432727088E-4	0.0	0.0	28.38531011667469	0.0
28	1.8104069432727088E-4	0.0	0.0	28.814798990517087	0.0
29	1.8104069432727088E-4	0.0	0.0	29.27765969901381	0.0
30	1.8104069432727088E-4	0.0	0.0	29.81903172195046	0.0
31	2.4138759243636117E-4	0.0	0.0	30.265055645874746	0.0
32	2.4138759243636117E-4	0.0	0.0	30.707398409014377	0.0
33	2.4138759243636117E-4	0.0	0.0	31.140689137437647	0.0
34	2.4138759243636117E-4	0.0	0.0	31.58689410205626	0.0
35	2.4138759243636117E-4	0.0	0.0	32.05192729888491	0.0
36	2.4138759243636117E-4	0.0	0.0	32.48153686652353	0.0
37	2.4138759243636117E-4	0.0	0.0	32.928526340817555	0.0
38	2.4138759243636117E-4	0.0	0.0	33.407016895924535	0.0
39	2.4138759243636117E-4	0.0	0.0	34.05417703124642	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGC	35	1.2123564E-7	45.0	43
TACGGCT	4060	0.0	42.50616	7
ATTAGCG	90	0.0	42.5	1
CGTTTTT	7305	0.0	41.88912	1
TGATACC	4080	0.0	41.47059	4
CGATGAA	1185	0.0	41.20253	19
ACGGCTG	4250	0.0	40.711765	8
GATACCT	4190	0.0	40.43556	5
CACAACG	425	0.0	39.705883	12
CCGATAT	40	3.4594632E-7	39.375	11
CGGTCGA	115	0.0	39.130436	41
GAATGAT	14460	0.0	39.040455	1
CCGATGA	1225	0.0	38.7551	18
GATGAAT	1285	0.0	38.6965	20
ATGATAC	14115	0.0	38.512222	3
GAATCTG	4745	0.0	38.50369	1
GAATGAC	1070	0.0	38.271027	1
AATGATA	14345	0.0	37.988846	2
CGTTCGG	255	0.0	37.941177	45
GTCGAAT	125	0.0	37.8	43
>>END_MODULE