##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	735927
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20129713952607	33.0	31.0	34.0	30.0	34.0
2	32.36865069497382	34.0	31.0	34.0	30.0	34.0
3	32.36748074197577	34.0	31.0	34.0	30.0	34.0
4	35.918231020196295	37.0	35.0	37.0	35.0	37.0
5	35.804549907803356	37.0	35.0	37.0	35.0	37.0
6	35.74542176058223	37.0	35.0	37.0	35.0	37.0
7	35.994790244141065	37.0	35.0	37.0	35.0	37.0
8	35.96065506497248	37.0	35.0	37.0	35.0	37.0
9	37.47998646604894	39.0	37.0	39.0	35.0	39.0
10	37.27763623294158	39.0	37.0	39.0	34.0	39.0
11	37.19647600916939	39.0	37.0	39.0	34.0	39.0
12	37.093122008025254	39.0	37.0	39.0	34.0	39.0
13	37.015414572369274	39.0	37.0	39.0	33.0	39.0
14	38.107471257339384	40.0	37.0	41.0	33.0	41.0
15	38.20650553655458	40.0	37.0	41.0	33.0	41.0
16	38.226371637404256	40.0	37.0	41.0	34.0	41.0
17	38.24617387322384	40.0	37.0	41.0	34.0	41.0
18	38.19838516591999	40.0	37.0	41.0	34.0	41.0
19	38.15558064862412	40.0	37.0	41.0	34.0	41.0
20	38.10954075608043	40.0	37.0	41.0	34.0	41.0
21	38.07513109316549	40.0	37.0	41.0	34.0	41.0
22	37.96028682192663	40.0	36.0	41.0	33.0	41.0
23	37.69745232883153	40.0	36.0	41.0	33.0	41.0
24	37.49301493218757	39.0	36.0	41.0	32.0	41.0
25	37.53040858672124	39.0	36.0	41.0	33.0	41.0
26	37.639212856709975	39.0	36.0	41.0	33.0	41.0
27	37.71368899360942	40.0	36.0	41.0	33.0	41.0
28	37.68282995460147	40.0	36.0	41.0	33.0	41.0
29	37.67691360692025	40.0	36.0	41.0	33.0	41.0
30	37.604066707703346	40.0	36.0	41.0	33.0	41.0
31	37.45761060539972	40.0	36.0	41.0	33.0	41.0
32	37.37935963757275	39.0	36.0	41.0	33.0	41.0
33	37.29277360390365	39.0	35.0	41.0	33.0	41.0
34	37.22302755572224	39.0	35.0	41.0	32.0	41.0
35	37.16403121505258	39.0	35.0	41.0	32.0	41.0
36	37.0870262947276	39.0	35.0	41.0	32.0	41.0
37	36.99238375545401	39.0	35.0	41.0	31.0	41.0
38	36.87541155576572	39.0	35.0	41.0	31.0	41.0
39	36.85901590782782	39.0	35.0	41.0	31.0	41.0
40	36.774184124240584	39.0	35.0	41.0	31.0	41.0
41	36.72304589993301	39.0	35.0	41.0	31.0	41.0
42	36.690461146282175	39.0	35.0	41.0	31.0	41.0
43	36.585973880561525	39.0	35.0	41.0	31.0	41.0
44	36.495534203800105	39.0	35.0	41.0	31.0	41.0
45	36.44054233640021	39.0	35.0	40.0	31.0	41.0
46	36.34232471427193	39.0	35.0	40.0	31.0	41.0
47	36.217855847115274	38.0	35.0	40.0	30.0	41.0
48	36.15098372528797	38.0	35.0	40.0	30.0	41.0
49	36.114861936034416	38.0	35.0	40.0	30.0	41.0
50	36.01485609306358	38.0	35.0	40.0	30.0	41.0
51	34.97094277013888	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	3.0
11	5.0
12	9.0
13	11.0
14	19.0
15	18.0
16	52.0
17	109.0
18	191.0
19	361.0
20	643.0
21	1034.0
22	1538.0
23	2208.0
24	2987.0
25	3973.0
26	4874.0
27	5864.0
28	7061.0
29	8572.0
30	10766.0
31	14091.0
32	18180.0
33	25893.0
34	46307.0
35	60398.0
36	58706.0
37	89285.0
38	159435.0
39	213207.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78977942105671	22.29188492880408	24.931141268087732	11.98719438205148
2	31.971785245003918	24.42619988123822	25.43472382450977	18.167291049248092
3	32.07886108268891	24.260014919958095	25.925805140999042	17.73531885635396
4	29.334295385275986	25.022998204985004	26.05204048771142	19.590665922027593
5	25.147738838227163	29.88598053883062	24.72093020095743	20.24535042198479
6	26.335220748797095	33.81544636900127	24.66888699558516	15.180445886616473
7	77.40713413151032	6.671177983685881	9.927750986171183	5.993936898632609
8	76.20687921492213	5.740379140865874	10.780552962454157	7.272188681757838
9	69.74985290660622	7.939782070776041	13.72785615964627	8.582508862971464
10	37.70319610504846	24.581650082141298	20.758988323570136	16.956165489240103
11	28.88139720379875	25.49111528724996	25.407547215960278	20.21994029299102
12	26.20776245469999	22.31783858996884	31.473502127249038	20.000896828082134
13	24.073855151394092	24.05388034411022	32.0897317261087	19.782532778386987
14	18.815860812281652	29.17245868136378	29.63595574017532	22.375724766179253
15	17.849868261390057	26.16563871144828	35.69185530630076	20.292637720860903
16	20.198199006151427	25.82158284721175	31.85709995692508	22.12311818971175
17	19.934178254093137	25.135917013508134	28.23092507816672	26.69897965423201
18	20.60082046181211	26.745044005723393	30.134782390101194	22.519353142363304
19	21.977043918758245	27.075239799599686	28.238806294646075	22.70890998699599
20	23.957811032887776	27.071570957445505	27.827895973377792	21.142722036288923
21	22.768290876676627	27.226069977049356	28.788996734730482	21.21664241154354
22	19.72220070740712	26.442024820396586	27.27091138115601	26.56486309104028
23	20.461540342995978	26.554400096748726	27.87722151789512	25.10683804236018
24	22.422604415927122	24.964432613560856	28.448066180477138	24.16489679003488
25	20.922047974867073	26.270676303492056	26.246489121883016	26.560786599757858
26	18.95228738720009	27.173755005591588	28.302127792566385	25.571829814641937
27	21.41856461306624	26.971017505812394	28.493315233712043	23.11710264740932
28	18.32274125015117	27.25936132252248	29.702810197207057	24.71508723011929
29	20.091938466722922	25.476983450804227	28.503234695832603	25.927843386640255
30	22.10504574502634	26.67166716263977	28.344795067989082	22.87849202434481
31	21.507024473894827	29.30385758370056	24.582873029526027	24.606244912878587
32	21.886953461416688	27.2608560359927	25.858950683967297	24.993239818623316
33	22.870882573950947	27.319965159587838	26.39310692500751	23.416045341453703
34	21.876082817996895	25.68298214360935	27.477181840046633	24.96375319834712
35	20.949768115587553	23.786870165111484	27.757100908106374	27.50626081119459
36	23.105552588775787	26.921420195209578	26.899135376198995	23.073891839815634
37	22.60631828972167	27.22987470224628	28.83438167100813	21.329425337023917
38	20.88019599770086	28.109037988822262	26.9630004062903	24.047765607186584
39	24.961443186620414	25.25100995071522	27.080811004352334	22.706735858312033
40	23.73917521710713	23.241435631523235	29.11226249342666	23.907126657942975
41	21.208489428978687	24.1294313158778	26.54189885681596	28.12018039832755
42	23.102019629664355	23.881580645906457	27.62583788881234	25.390561835616847
43	23.45341317820925	23.616065180377944	29.040108597727766	23.890413043685037
44	21.356737828616154	25.098141527624346	27.735631387352278	25.809489256407225
45	21.672122370832977	22.840037123247278	28.365856939614936	27.12198356630481
46	23.268476357029975	24.41600865303216	28.002505683308264	24.3130093066296
47	20.868238289939082	24.04300970069042	30.037354248451276	25.05139776091922
48	20.411671266307664	22.21511100965177	30.239548216059475	27.13366950798109
49	22.692875787951795	21.598337878621113	31.651237147162693	24.0575491862644
50	20.672974357511002	21.385137384550372	30.848576013653528	27.0933122442851
51	20.498500530623282	20.968791741572193	27.699622381024202	30.83308534678032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	815.0
1	1188.5
2	1562.0
3	2105.5
4	2649.0
5	2010.5
6	1372.0
7	1347.0
8	1322.0
9	1436.0
10	1550.0
11	1573.5
12	1597.0
13	1630.5
14	1664.0
15	1701.5
16	1739.0
17	1817.0
18	1895.0
19	1948.0
20	2001.0
21	2056.0
22	2111.0
23	2407.0
24	2703.0
25	3392.5
26	5028.5
27	5975.0
28	6877.0
29	7779.0
30	9076.0
31	10373.0
32	11350.0
33	12327.0
34	14019.5
35	15712.0
36	16869.5
37	18027.0
38	19950.5
39	21874.0
40	25192.5
41	28511.0
42	33194.0
43	37877.0
44	44028.0
45	50179.0
46	60706.5
47	71234.0
48	81559.0
49	91884.0
50	88553.5
51	85223.0
52	71100.5
53	56978.0
54	48449.0
55	39920.0
56	35771.5
57	31623.0
58	30236.5
59	28850.0
60	28083.5
61	27317.0
62	24403.0
63	21489.0
64	18473.5
65	15458.0
66	13283.0
67	11108.0
68	9125.0
69	7142.0
70	5815.0
71	4488.0
72	3753.0
73	3018.0
74	2548.0
75	1579.0
76	1080.0
77	911.0
78	742.0
79	547.0
80	352.0
81	217.5
82	83.0
83	70.0
84	57.0
85	42.0
86	27.0
87	32.0
88	37.0
89	35.5
90	34.0
91	19.5
92	5.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	735927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.50389189053234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.65290978312784	21.24684829885534
2	12.665386815342181	7.7268718033394315
3	4.870200895563965	4.456802448103709
4	2.396350274052802	2.923920387662169
5	1.3816965946043893	2.1073561773664493
6	0.9500117915425198	1.7387434190366386
7	0.684338242970115	1.461248584608207
8	0.526363114531249	1.284489883266009
9	0.4118707394374017	1.1307294457804908
>10	6.146228220913176	43.72369380356645
>50	0.23226904209083882	4.4075973438528715
>100	0.07427207738187241	4.317660387189556
>500	0.004951471825458161	1.0559010455553408
>1k	0.003150936616200648	2.418136971817309
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT	4207	0.5716599608385071	No Hit
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	3894	0.5291285684585564	No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	3776	0.5130943694143577	No Hit
TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	1691	0.2297782252859319	No Hit
ACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	1443	0.1960792306845652	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCC	1400	0.19023625984642498	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1200	0.1630596512969357	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	985	0.13384479710623473	No Hit
GCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	946	0.12854535843908432	No Hit
AGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	866	0.1176747150192886	No Hit
GGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	791	0.10748348681323011	No Hit
ACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	747	0.10150463293234248	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3588304274744642E-4	0.0	0.0	0.6080766162948227	0.0
2	1.3588304274744642E-4	0.0	0.0	2.1776616430705764	0.0
3	1.3588304274744642E-4	0.0	0.0	3.1349576792263365	0.0
4	1.3588304274744642E-4	0.0	0.0	4.197698956554115	0.0
5	1.3588304274744642E-4	0.0	0.0	6.7126223117238535	0.0
6	1.3588304274744642E-4	0.0	0.0	8.031639007673315	0.0
7	1.3588304274744642E-4	0.0	0.0	9.440066745750597	0.0
8	1.3588304274744642E-4	0.0	0.0	11.596394751109825	0.0
9	1.3588304274744642E-4	0.0	0.0	12.374053404753461	0.0
10	1.3588304274744642E-4	0.0	0.0	14.00777522770601	0.0
11	1.3588304274744642E-4	0.0	0.0	16.352980662484185	0.0
12	1.3588304274744642E-4	0.0	0.0	18.082635913616432	0.0
13	1.3588304274744642E-4	0.0	0.0	18.789635385031396	0.0
14	1.3588304274744642E-4	0.0	0.0	19.072136230903336	0.0
15	1.3588304274744642E-4	0.0	0.0	19.639312051331178	0.0
16	1.3588304274744642E-4	0.0	0.0	20.75980362182662	0.0
17	1.3588304274744642E-4	0.0	0.0	22.128961160549892	0.0
18	1.3588304274744642E-4	0.0	0.0	23.733060480183497	0.0
19	1.3588304274744642E-4	0.0	0.0	24.589667181663398	0.0
20	1.3588304274744642E-4	0.0	0.0	25.440838561433402	0.0
21	1.3588304274744642E-4	0.0	0.0	26.419196469215017	0.0
22	1.3588304274744642E-4	0.0	0.0	27.38804256400431	0.0
23	1.3588304274744642E-4	0.0	0.0	28.27563059923063	0.0
24	1.3588304274744642E-4	0.0	0.0	29.020949088700373	0.0
25	1.3588304274744642E-4	0.0	0.0	29.659871155698866	0.0
26	1.3588304274744642E-4	0.0	0.0	30.249739444265533	0.0
27	1.3588304274744642E-4	0.0	0.0	30.834444177207793	0.0
28	1.3588304274744642E-4	0.0	0.0	31.43491134310876	0.0
29	1.3588304274744642E-4	0.0	0.0	32.060788638003494	0.0
30	1.3588304274744642E-4	0.0	0.0	32.72552848312401	0.0
31	1.3588304274744642E-4	0.0	0.0	33.38537653870561	0.0
32	1.3588304274744642E-4	0.0	0.0	34.009623237087375	0.0
33	1.3588304274744642E-4	0.0	0.0	34.585495572251055	0.0
34	1.3588304274744642E-4	0.0	0.0	35.17400503039024	0.0
35	1.3588304274744642E-4	0.0	0.0	35.74498557601501	0.0
36	1.3588304274744642E-4	0.0	0.0	36.3337668002397	0.0
37	1.3588304274744642E-4	0.0	0.0	36.913172094514806	0.0
38	1.3588304274744642E-4	0.0	0.0	37.46893373935187	0.0
39	1.3588304274744642E-4	0.0	0.0	38.02700539591563	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGC	20	7.03163E-4	45.000004	27
CGTATAT	20	7.03163E-4	45.000004	30
TTGTGCG	20	7.03163E-4	45.000004	1
GTTAACG	20	7.03163E-4	45.000004	1
CATCGTT	20	7.03163E-4	45.000004	36
CATCGAT	20	7.03163E-4	45.000004	13
CGGCTAC	20	7.03163E-4	45.000004	25
TTACGCG	20	7.03163E-4	45.000004	1
CGGTCTA	40	6.8084773E-9	45.000004	31
AATAGGC	20	7.03163E-4	45.000004	18
TCGACTA	20	7.03163E-4	45.000004	28
TGCGTAG	40	6.8084773E-9	45.000004	1
CCCGAAC	20	7.03163E-4	45.000004	33
CTAACGG	25	3.889433E-5	45.0	2
TGATTCG	50	2.1827873E-11	45.0	15
ACGTCAT	25	3.889433E-5	45.0	35
TATCGAC	25	3.889433E-5	45.0	28
CAATAAG	25	3.889433E-5	45.0	38
TGGCACG	30	2.1644337E-6	44.999996	1
TACGCGC	30	2.1644337E-6	44.999996	34
>>END_MODULE