FastQCFastQC Report
Sat 18 Jun 2016
SRR3550017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179772
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44880.38041248648043857No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT26080.22105966237544206No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC25300.2144482154178943No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC22260.18868052471155441No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC20860.1768138250441611No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG13350.11315745754264382No Hit
TCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC12410.10518981633739402No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATG207.033362E-445.01
TCGATAG302.1655505E-644.9999961
TCGTAAG302.1655505E-644.9999961
TACGAAT2000.040.512
CGAATAT2000.040.514
CGTTTTT25600.040.1660161
GCCGATA403.4583172E-739.3759
CTCGATC1600.039.37516
CGTAAGG1050.038.571432
GACGTAG356.2484287E-638.5714261
GTACGAG1000.038.251
TGATTCG659.094947E-1238.0769215
CTACGAA2150.037.67441611
CCGAACC900.037.518
GTAATCG301.1399402E-437.4999961
CACGTAG552.750312E-936.818181
TAAGACG552.750312E-936.818181
TCGATCA1550.036.2903217
TATCACG250.002107131336.01
AACCCGC1000.036.021
Produced by FastQC (version 0.11.5)