##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550017_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1179772
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29502056329528	33.0	31.0	34.0	30.0	34.0
2	32.47161231153138	34.0	31.0	34.0	31.0	34.0
3	32.45386481455739	34.0	31.0	34.0	30.0	34.0
4	35.99863702478106	37.0	35.0	37.0	35.0	37.0
5	35.87340096221982	37.0	35.0	37.0	35.0	37.0
6	35.82263522104271	37.0	35.0	37.0	35.0	37.0
7	36.03291568201313	37.0	35.0	37.0	35.0	37.0
8	36.019448673133454	37.0	35.0	37.0	35.0	37.0
9	37.59553879902219	39.0	38.0	39.0	35.0	39.0
10	37.36238442682146	39.0	37.0	39.0	34.0	39.0
11	37.21956191535313	39.0	37.0	39.0	34.0	39.0
12	36.96104755834178	39.0	35.0	39.0	33.0	39.0
13	36.83070711968075	39.0	35.0	39.0	33.0	39.0
14	37.67267573734586	40.0	37.0	41.0	33.0	41.0
15	37.860061944172266	40.0	37.0	41.0	33.0	41.0
16	37.98716785955252	40.0	37.0	41.0	33.0	41.0
17	38.06428021685546	40.0	37.0	41.0	33.0	41.0
18	38.02024119914695	40.0	36.0	41.0	33.0	41.0
19	37.95506758933082	40.0	36.0	41.0	33.0	41.0
20	37.86117571869819	40.0	36.0	41.0	33.0	41.0
21	37.85496604428652	40.0	36.0	41.0	33.0	41.0
22	37.823132774807334	40.0	35.0	41.0	33.0	41.0
23	37.6014585869134	39.0	35.0	41.0	33.0	41.0
24	37.370229162923	39.0	35.0	41.0	32.0	41.0
25	37.366824267739865	39.0	35.0	41.0	32.0	41.0
26	37.44551320085576	39.0	35.0	41.0	33.0	41.0
27	37.51897485276816	39.0	35.0	41.0	33.0	41.0
28	37.49981691377656	39.0	35.0	41.0	33.0	41.0
29	37.44206422935957	39.0	35.0	41.0	33.0	41.0
30	37.37699741984044	39.0	35.0	41.0	33.0	41.0
31	37.19203625785321	39.0	35.0	41.0	32.0	41.0
32	37.06454382711236	39.0	35.0	41.0	32.0	41.0
33	36.99731473538955	39.0	35.0	41.0	32.0	41.0
34	36.85812512926226	39.0	35.0	41.0	31.0	41.0
35	36.751545213820975	39.0	35.0	41.0	31.0	41.0
36	36.676680748483605	39.0	35.0	41.0	31.0	41.0
37	36.655352051074274	39.0	35.0	41.0	31.0	41.0
38	36.54078160864981	39.0	35.0	41.0	31.0	41.0
39	36.467720034040475	39.0	35.0	41.0	31.0	41.0
40	36.3670768589185	39.0	35.0	41.0	31.0	41.0
41	36.24313087613539	39.0	35.0	41.0	30.0	41.0
42	36.231638825128925	39.0	35.0	41.0	30.0	41.0
43	36.187868503405745	38.0	35.0	40.0	30.0	41.0
44	36.11419155565652	38.0	35.0	40.0	30.0	41.0
45	36.0536349396324	38.0	35.0	40.0	30.0	41.0
46	35.96050253777849	38.0	35.0	40.0	30.0	41.0
47	35.83182089420668	38.0	35.0	40.0	30.0	41.0
48	35.73624649508549	38.0	35.0	40.0	29.0	41.0
49	35.71426258633024	38.0	35.0	40.0	29.0	41.0
50	35.618316929033746	37.0	35.0	40.0	29.0	41.0
51	34.585191036912214	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	10.0
10	14.0
11	16.0
12	12.0
13	19.0
14	24.0
15	63.0
16	134.0
17	187.0
18	395.0
19	698.0
20	1064.0
21	1775.0
22	2526.0
23	3767.0
24	5463.0
25	7561.0
26	9788.0
27	11542.0
28	13338.0
29	15574.0
30	19132.0
31	24404.0
32	32861.0
33	49000.0
34	89955.0
35	91388.0
36	91338.0
37	140223.0
38	238439.0
39	328848.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.37746276399169	21.464740644802554	26.020197122833906	12.137599468371855
2	33.831706465317026	24.01506392760635	24.269265586910013	17.88396402016661
3	31.579322106305284	23.607781842593315	27.195424200608254	17.617471850493146
4	28.93728618750064	25.24869212017237	26.39501530804257	19.419006384284422
5	25.734887757973574	28.82463730280088	25.794814591293914	19.645660347931635
6	27.616013941676865	32.530099036084934	24.709265858148864	15.144621164089333
7	78.99263586523497	6.150256151188534	9.40995378768101	5.447154195895478
8	79.47688197380512	5.043516882923141	9.974978216129896	5.504622927141854
9	73.82401006296132	6.669932834479883	12.267116019027405	7.238941083531395
10	43.77142363100667	21.945681029893912	19.054105369512076	15.228789969587345
11	33.406370044381454	23.25720562956232	23.83757200543834	19.498852320617882
12	29.34287302970404	21.25936197841617	29.413225606303588	19.9845393855762
13	27.283153016006484	21.666389777007762	31.575592572124105	19.474864634861653
14	20.770877762821968	24.72757448049284	33.58292958300417	20.91861817368102
15	20.030565227857586	23.706699260535085	36.00458393655723	20.258151575050096
16	25.43262596501697	23.29636573846472	30.89478305977765	20.37622523674066
17	25.822870859793245	23.211264549421415	27.816052593212927	23.14981199757241
18	25.931366399609416	23.34671444991066	29.493410591198977	21.228508559280947
19	25.784049799452774	25.68428476010619	27.114561118589016	21.41710432185202
20	24.78936608090377	27.845804104521893	26.999708418236747	20.3651213963376
21	23.32942297325246	25.980952251791024	30.697711083158442	19.99191369179808
22	21.698345103969242	23.90071980009697	30.329165296345394	24.071769799588395
23	22.30592012693978	24.75029073414185	30.510556276975553	22.433232861942816
24	23.124213831147035	25.142485158149203	28.54424414208847	23.189056868615292
25	24.631030402484548	24.781737488260443	26.29491122013406	24.29232088912095
26	21.702074638150425	25.508996653590692	27.109899200862543	25.679029507396343
27	21.99102877505145	24.631538975327434	29.424838019549536	23.952594230071572
28	20.82546458129198	25.80329080534205	27.14727930481483	26.223965308551143
29	22.385935587554208	26.935882526454265	26.241765358052234	24.436416527939297
30	25.38295535069488	24.762581244511654	25.981799873195836	23.87266353159763
31	24.77343079849327	25.653007530268564	25.541121504833136	24.032440166405035
32	25.36990198106075	26.422817290120467	23.913857931871583	24.293422796947205
33	25.15392804711419	25.752603045334183	24.529061547485444	24.56440736006618
34	21.843457888473367	25.56536347701081	27.951502493702172	24.63967614081365
35	21.206300878474824	28.422610470497688	25.204700569262535	25.16638808176495
36	23.09369946057374	28.73843420593132	24.7090963338679	23.458769999627048
37	22.16224829882384	28.291483439172993	25.451697446625282	24.094570815377885
38	23.14904913830808	27.17677652970235	25.145621357347014	24.528552974642558
39	22.215309398765186	24.914559762394767	26.584967264861348	26.285163573978704
40	23.09564898980481	24.690618187242958	27.780282969929782	24.43344985302245
41	20.278409726625142	25.825074675445762	26.5610643412456	27.335451256683495
42	20.84453606290029	25.507979507904917	29.313375804816523	24.33410862437827
43	21.74615095120074	23.414863210857693	29.574358435358693	25.264627402582875
44	22.814069159125662	23.702461153511017	26.931983467992122	26.5514862193712
45	21.72292612470884	23.083273717294528	26.446211640893324	28.747588517103306
46	21.765052908528087	24.369454436959007	27.04437806627043	26.821114588242473
47	19.905117259945143	25.357611470690948	29.748629396188413	24.988641873175496
48	20.286970702813765	25.656991350871184	28.666810197224546	25.389227749090505
49	22.874419803148406	23.40731938035485	28.86769647016542	24.850564346331325
50	19.923002071586712	24.165262440539358	28.38107702166181	27.530658466212117
51	19.58446208250408	24.57169690414758	26.68354563424119	29.160295379107147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	666.0
1	1445.0
2	2224.0
3	4874.0
4	7524.0
5	5075.0
6	2626.0
7	2528.5
8	2431.0
9	2535.5
10	2640.0
11	2676.0
12	2712.0
13	2756.0
14	2800.0
15	2937.5
16	3075.0
17	3033.5
18	2992.0
19	3091.5
20	3191.0
21	3404.0
22	3617.0
23	4109.5
24	4602.0
25	5362.5
26	7655.5
27	9188.0
28	10372.5
29	11557.0
30	13830.5
31	16104.0
32	17843.0
33	19582.0
34	22872.5
35	26163.0
36	27890.0
37	29617.0
38	32396.5
39	35176.0
40	39054.5
41	42933.0
42	47748.5
43	52564.0
44	58966.0
45	65368.0
46	77685.0
47	90002.0
48	103348.0
49	116694.0
50	116654.0
51	116614.0
52	103555.5
53	90497.0
54	82704.5
55	74912.0
56	70738.0
57	66564.0
58	64189.5
59	61815.0
60	57478.5
61	53142.0
62	48744.5
63	44347.0
64	39722.0
65	35097.0
66	30216.0
67	25335.0
68	21410.5
69	17486.0
70	14904.0
71	12322.0
72	10238.5
73	8155.0
74	6709.0
75	4125.0
76	2987.0
77	2246.5
78	1506.0
79	1195.0
80	884.0
81	603.5
82	323.0
83	230.0
84	137.0
85	116.5
86	96.0
87	80.0
88	64.0
89	55.0
90	46.0
91	25.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1179772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.2532607288533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88507178350174	23.185279627456172
2	10.425433593293718	6.725084557916965
3	3.790957420301967	3.668122142669414
4	2.015247363410599	2.5999319458086494
5	1.3014047595757325	2.0987273512183373
6	0.9761857130959781	1.88911033945597
7	0.7774550130841341	1.755282146936964
8	0.6441846999971991	1.6621645669238245
9	0.59637631275731	1.7311572637086314
>10	7.450870078258837	47.768472765660576
>50	0.0878390048625117	1.8614119482867757
>100	0.04285247564200097	2.6893199563231356
>500	0.003992466664069225	0.8556341802813144
>1k	0.0021293155541702528	1.5103012073531823
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4488	0.38041248648043857	No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT	2608	0.22105966237544206	No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	2530	0.2144482154178943	No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	2226	0.18868052471155441	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC	2086	0.1768138250441611	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1335	0.11315745754264382	No Hit
TCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	1241	0.10518981633739402	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.695242809627623E-4	0.0	0.0	0.23479112913342579	0.0
2	1.695242809627623E-4	0.0	0.0	0.8607595365884255	0.0
3	1.695242809627623E-4	0.0	0.0	1.3608561654285742	0.0
4	1.695242809627623E-4	0.0	0.0	1.9597006879295322	0.0
5	1.695242809627623E-4	0.0	0.0	3.353190277443438	0.0
6	1.695242809627623E-4	0.0	0.0	4.332616810705797	0.0
7	1.695242809627623E-4	0.0	0.0	5.3371329375506456	0.0
8	1.695242809627623E-4	0.0	0.0	6.827166605072844	0.0
9	1.695242809627623E-4	0.0	0.0	7.44796452195848	0.0
10	1.695242809627623E-4	0.0	0.0	8.542667566275517	0.0
11	1.695242809627623E-4	0.0	0.0	10.21883889429483	0.0
12	1.695242809627623E-4	0.0	0.0	11.400423132605283	0.0
13	1.695242809627623E-4	0.0	0.0	11.891958785256813	0.0
14	1.695242809627623E-4	0.0	0.0	12.124800385159165	0.0
15	1.695242809627623E-4	0.0	0.0	12.503771915251422	0.0
16	1.695242809627623E-4	0.0	0.0	13.207806254089773	0.0
17	1.695242809627623E-4	0.0	0.0	14.023472331942104	0.0
18	1.695242809627623E-4	0.0	0.0	14.907202408600984	0.0
19	1.695242809627623E-4	0.0	0.0	15.491298318658181	0.0
20	1.695242809627623E-4	0.0	0.0	16.113537191932	0.0
21	1.695242809627623E-4	0.0	0.0	16.850374479136647	0.0
22	1.695242809627623E-4	0.0	0.0	17.609673733568858	0.0
23	1.695242809627623E-4	0.0	0.0	18.326083344917492	0.0
24	1.695242809627623E-4	0.0	0.0	18.907805915041212	0.0
25	1.695242809627623E-4	0.0	0.0	19.417989238598643	0.0
26	1.695242809627623E-4	0.0	0.0	19.92164587733901	0.0
27	1.695242809627623E-4	0.0	0.0	20.53278091020977	0.0
28	1.695242809627623E-4	0.0	0.0	21.060170948284924	0.0
29	1.695242809627623E-4	0.0	0.0	21.660541189314547	0.0
30	2.5428642144414347E-4	0.0	0.0	22.284051494695586	0.0
31	2.5428642144414347E-4	0.0	0.0	22.816272974778176	0.0
32	2.5428642144414347E-4	0.0	0.0	23.359513533123348	0.0
33	2.5428642144414347E-4	0.0	0.0	23.893006445313162	0.0
34	2.5428642144414347E-4	0.0	0.0	24.463879461455264	0.0
35	2.5428642144414347E-4	0.0	0.0	25.04543250729802	0.0
36	2.5428642144414347E-4	0.0	0.0	25.548326286774056	0.0
37	2.5428642144414347E-4	0.0	0.0	26.12742123054285	0.0
38	2.5428642144414347E-4	0.0	0.0	26.788735450578585	0.0
39	2.5428642144414347E-4	0.0	0.0	27.881743252085997	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATG	20	7.033362E-4	45.0	1
TCGATAG	30	2.1655505E-6	44.999996	1
TCGTAAG	30	2.1655505E-6	44.999996	1
TACGAAT	200	0.0	40.5	12
CGAATAT	200	0.0	40.5	14
CGTTTTT	2560	0.0	40.166016	1
GCCGATA	40	3.4583172E-7	39.375	9
CTCGATC	160	0.0	39.375	16
CGTAAGG	105	0.0	38.57143	2
GACGTAG	35	6.2484287E-6	38.571426	1
GTACGAG	100	0.0	38.25	1
TGATTCG	65	9.094947E-12	38.07692	15
CTACGAA	215	0.0	37.674416	11
CCGAACC	90	0.0	37.5	18
GTAATCG	30	1.1399402E-4	37.499996	1
CACGTAG	55	2.750312E-9	36.81818	1
TAAGACG	55	2.750312E-9	36.81818	1
TCGATCA	155	0.0	36.29032	17
TATCACG	25	0.0021071313	36.0	1
AACCCGC	100	0.0	36.0	21
>>END_MODULE