FastQCFastQC Report
Sat 18 Jun 2016
SRR3550014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928146
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50580.2623245335156155No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC49240.2553748523192746No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT46430.2408012671239626No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC35420.18369978207044488No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC25440.13194021614545787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGG603.6379788E-1241.2499962
TCGTTAG501.0822987E-940.5000041
CTACGAA1350.040.011
CGAATAT1250.039.60000214
CTAACGG1450.038.7931062
TCGATAG659.094947E-1238.076921
CGTAAGG2900.038.0172422
CGTTAGG2400.037.4999962
CGATACG601.5643309E-1037.49999610
GTACGAG1850.036.4864851
CGTTTTT29050.036.32531
ATAGGGC8150.036.1656464
ATAGCGG2800.036.1607172
ATCGCTC250.002107461436.00000430
TACGTTG504.8818038E-836.0000041
ACGTAGG1950.035.769232
TATAGCG1200.035.6251
ATAACGG950.035.5263142
GAATGCG1150.035.217391
TAAGGGA11650.035.150214
Produced by FastQC (version 0.11.5)