##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550014_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1928146
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35918701177193	33.0	31.0	34.0	30.0	34.0
2	32.518596620795314	34.0	31.0	34.0	31.0	34.0
3	32.5177616217859	34.0	31.0	34.0	31.0	34.0
4	36.040047278577454	37.0	35.0	37.0	35.0	37.0
5	35.93039946145157	37.0	35.0	37.0	35.0	37.0
6	35.88705782653388	37.0	35.0	37.0	35.0	37.0
7	36.092901678607326	37.0	35.0	37.0	35.0	37.0
8	36.052592490402695	37.0	35.0	37.0	35.0	37.0
9	37.567314404614585	39.0	38.0	39.0	35.0	39.0
10	37.39201751319661	39.0	37.0	39.0	34.0	39.0
11	37.32285521946989	39.0	37.0	39.0	34.0	39.0
12	37.227944875543656	39.0	37.0	39.0	34.0	39.0
13	37.20010829055476	39.0	37.0	39.0	34.0	39.0
14	38.39356770700974	40.0	38.0	41.0	34.0	41.0
15	38.477845038705574	40.0	38.0	41.0	34.0	41.0
16	38.488127973711535	40.0	38.0	41.0	34.0	41.0
17	38.49840364785654	40.0	38.0	41.0	34.0	41.0
18	38.441504429643814	40.0	38.0	41.0	34.0	41.0
19	38.38812932215714	40.0	37.0	41.0	34.0	41.0
20	38.34645716662535	40.0	37.0	41.0	34.0	41.0
21	38.32498991258961	40.0	37.0	41.0	34.0	41.0
22	38.233719853164644	40.0	37.0	41.0	34.0	41.0
23	37.97721282516988	40.0	37.0	41.0	33.0	41.0
24	37.77421056289306	40.0	36.0	41.0	33.0	41.0
25	37.762983715963415	40.0	36.0	41.0	33.0	41.0
26	37.8491753217858	40.0	36.0	41.0	33.0	41.0
27	37.90477795768577	40.0	36.0	41.0	33.0	41.0
28	37.878991010016875	40.0	36.0	41.0	33.0	41.0
29	37.88633951993262	40.0	36.0	41.0	33.0	41.0
30	37.8115858446404	40.0	36.0	41.0	33.0	41.0
31	37.70626601927447	40.0	36.0	41.0	33.0	41.0
32	37.62389466357838	40.0	36.0	41.0	33.0	41.0
33	37.54835940846803	40.0	36.0	41.0	33.0	41.0
34	37.49408706602093	40.0	36.0	41.0	33.0	41.0
35	37.421170907182336	40.0	36.0	41.0	33.0	41.0
36	37.34175627779224	40.0	36.0	41.0	33.0	41.0
37	37.29173205763464	40.0	35.0	41.0	32.0	41.0
38	37.203382938843845	39.0	35.0	41.0	32.0	41.0
39	37.13866740381693	39.0	35.0	41.0	32.0	41.0
40	37.05860240873876	39.0	35.0	41.0	32.0	41.0
41	36.97587682675482	39.0	35.0	41.0	32.0	41.0
42	36.90533445081441	39.0	35.0	41.0	32.0	41.0
43	36.817194859725355	39.0	35.0	41.0	31.0	41.0
44	36.72987263412625	39.0	35.0	41.0	31.0	41.0
45	36.67388672849463	39.0	35.0	41.0	31.0	41.0
46	36.570200078209844	39.0	35.0	41.0	31.0	41.0
47	36.46166576597415	39.0	35.0	41.0	31.0	41.0
48	36.38354668163095	39.0	35.0	40.0	31.0	41.0
49	36.33608295222457	38.0	35.0	40.0	31.0	41.0
50	36.25444598075042	38.0	35.0	40.0	31.0	41.0
51	35.257050036667344	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	12.0
10	34.0
11	25.0
12	21.0
13	17.0
14	37.0
15	64.0
16	106.0
17	228.0
18	397.0
19	674.0
20	1197.0
21	1974.0
22	3088.0
23	4568.0
24	6460.0
25	8918.0
26	12123.0
27	14985.0
28	17705.0
29	21333.0
30	26529.0
31	33307.0
32	43538.0
33	61047.0
34	110711.0
35	144074.0
36	145432.0
37	228014.0
38	407398.0
39	633710.0
40	417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.209798428127336	20.69423166088045	24.704145847876667	11.391824063115552
2	32.36673986306016	24.15698811189609	25.445998383939806	18.030273641103943
3	32.81426821412901	23.822521738499056	26.099683322735935	17.263526724635998
4	29.22029763306306	25.489044916723113	25.78814052462832	19.50251692558551
5	25.4827694583294	30.600431710046855	23.877652418437194	20.039146413186554
6	27.25592356595403	34.50241838532974	23.422448300076862	14.819209748639366
7	80.45531821760386	6.285623599042811	8.279196699834971	4.9798614835183646
8	80.6696692055477	5.483402190498023	8.449878795485404	5.397049808468861
9	73.61377198614628	7.995608216390253	11.196040133890275	7.194579663573195
10	39.028787239140605	27.19913326065557	17.756228003481063	16.015851496722757
11	29.365981621723662	24.52371345323435	25.344864963545295	20.765439961496693
12	26.94510685394156	21.7180649183205	29.967284635084688	21.369543592653255
13	24.67437631797592	22.9427128443593	31.296644548701185	21.086266288963596
14	20.685674217616302	26.48845056339095	29.795305957121503	23.030569261871246
15	20.485896814867754	25.345228006592862	32.86333088884348	21.305544289695906
16	22.96677741208394	24.352460861366307	30.897712102714216	21.78304962383554
17	22.484863698080954	24.905116106352942	28.305947786111634	24.304072409454474
18	22.83862321629171	25.527268163302985	29.166567262022692	22.46754135838261
19	24.10071643952273	26.80564646038215	26.87726966733847	22.21636743275665
20	25.202655815482856	25.84389356407658	27.310431886382048	21.64301873405852
21	24.73344860814482	26.069965656127696	27.656100730961246	21.540485004766236
22	22.868340882899947	24.36739748960919	27.17766185755643	25.586599769934438
23	22.945046692522247	25.47690890627577	27.887255425678347	23.69078897552364
24	23.528145690212256	24.276325547961616	28.618579713361953	23.576949048464172
25	23.195494532053072	25.751784356578806	26.305321277538113	24.747399833830013
26	22.038424476154812	26.29142191514543	26.946818342594387	24.723335266105366
27	22.919374362729794	25.921636639549078	27.594746455921904	23.56424254179922
28	20.842301360996522	26.387057826533884	28.375755777830104	24.394885034639493
29	21.953472403023422	25.243679679858268	27.32236044365935	25.48048747345896
30	22.777995027347515	25.79415666655948	27.283462974276844	24.14438533181616
31	22.655753246901426	26.317353561400438	26.498978811770478	24.52791437992766
32	22.651863499963177	26.167053739706432	26.190236631458408	24.990846128871983
33	22.37273525967432	26.2772632362902	26.569046119951494	24.780955384083985
34	20.891571488881027	24.45540949699867	29.499529599936935	25.153489414183365
35	21.591881527643654	24.294581426925138	27.765169235109788	26.348367810321417
36	21.86883150964709	26.72152419993092	26.43290497711273	24.976739313309263
37	21.547330959377557	26.571120651651896	29.25966187207815	22.621886516892392
38	21.70338760654017	25.890466800750566	27.475668336318932	24.930477256390336
39	23.19684297765833	23.507919006133353	28.07686762309493	25.218370393113382
40	21.79689712293571	23.321003699927285	29.956600796827626	24.925498380309374
41	20.542738983458722	24.900137230271984	27.49630992673791	27.060813859531386
42	21.872461940122793	25.42929840375158	27.498384458438313	25.19985519768731
43	22.643772826331617	23.73186470319156	28.695078069814215	24.929284400662606
44	21.579901107073844	24.249304772563903	28.317202120586305	25.85359199977595
45	21.924273369340288	22.648388659365008	28.506140095200262	26.921197876094443
46	21.734764898508725	24.51852712398335	27.964220551763198	25.782487425744733
47	20.653259659797545	24.260092337405982	30.003225896794124	25.083422106002345
48	20.266618814135445	23.49656094507366	29.831558398586	26.405261842204897
49	21.464245964776527	23.142230930645294	30.18609586618441	25.207427238393777
50	19.853787005755787	22.196555655017825	30.7193542397723	27.23030309945409
51	20.368063414285018	22.404890501030525	28.370102678946512	28.856943405737944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	870.0
1	1915.5
2	2961.0
3	6108.0
4	9255.0
5	6404.0
6	3553.0
7	3345.0
8	3137.0
9	3185.0
10	3233.0
11	3372.0
12	3511.0
13	3578.5
14	3646.0
15	3580.5
16	3515.0
17	3717.5
18	3920.0
19	4311.0
20	4702.0
21	4971.0
22	5240.0
23	6098.0
24	6956.0
25	8763.5
26	12958.0
27	15345.0
28	18783.5
29	22222.0
30	26103.0
31	29984.0
32	32787.0
33	35590.0
34	39441.5
35	43293.0
36	46782.0
37	50271.0
38	54683.0
39	59095.0
40	68499.0
41	77903.0
42	87934.0
43	97965.0
44	107641.0
45	117317.0
46	137535.5
47	157754.0
48	176846.5
49	195939.0
50	190700.5
51	185462.0
52	163001.5
53	140541.0
54	126520.0
55	112499.0
56	105387.0
57	98275.0
58	97150.0
59	96025.0
60	90858.0
61	85691.0
62	81408.0
63	77125.0
64	67453.5
65	57782.0
66	48768.5
67	39755.0
68	32708.0
69	25661.0
70	21227.0
71	16793.0
72	13702.0
73	10611.0
74	8543.0
75	5224.5
76	3974.0
77	2904.0
78	1834.0
79	1413.0
80	992.0
81	737.0
82	482.0
83	328.5
84	175.0
85	168.5
86	162.0
87	110.0
88	58.0
89	35.0
90	12.0
91	8.5
92	5.0
93	5.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1928146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.454751426686407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.58757922779091	18.673768622827414
2	11.336676776236386	5.998179322400444
3	4.324524217111561	3.4321263960711597
4	2.0997863703175175	2.2219730590357605
5	1.2773344157336457	1.6895782228492657
6	0.8306255705144714	1.3184395797965953
7	0.6448834557173706	1.1942162064129898
8	0.49564269783469744	1.048968349413533
9	0.418401235161321	0.996183060553018
>10	7.440595868530784	49.19304750782878
>50	0.46029747628952994	7.445660871116208
>100	0.07347064562983534	3.6319230292355753
>500	0.006987676659184914	1.3214124327006265
>1k	0.002994718568222106	1.567378206675878
>5k	1.996479045481404E-4	0.2671451330828478
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5058	0.2623245335156155	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	4924	0.2553748523192746	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	4643	0.2408012671239626	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	3542	0.18369978207044488	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCC	2544	0.13194021614545787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.186329251000702E-5	0.0	0.0	0.2590571460874851	0.0
2	5.186329251000702E-5	0.0	0.0	0.9031992390617722	0.0
3	5.186329251000702E-5	0.0	0.0	1.3690871956791655	0.0
4	5.186329251000702E-5	0.0	0.0	1.9138592202042792	0.0
5	1.0372658502001404E-4	0.0	0.0	3.198616702262173	0.0
6	1.0372658502001404E-4	0.0	0.0	4.109232392152877	0.0
7	1.0372658502001404E-4	0.0	0.0	4.9883670634900055	0.0
8	1.0372658502001404E-4	0.0	0.0	6.465900403807596	0.0
9	1.0372658502001404E-4	0.0	0.0	7.018296332331681	0.0
10	1.0372658502001404E-4	0.0	0.0	8.087509970717985	0.0
11	1.0372658502001404E-4	0.0	0.0	9.584077139386748	0.0
12	1.0372658502001404E-4	0.0	0.0	10.70914754380633	0.0
13	1.5558987753002106E-4	0.0	0.0	11.181310958817434	0.0
14	1.5558987753002106E-4	0.0	0.0	11.37320514110446	0.0
15	1.5558987753002106E-4	0.0	0.0	11.757460275311102	0.0
16	1.5558987753002106E-4	0.0	0.0	12.489510649089851	0.0
17	1.5558987753002106E-4	0.0	0.0	13.371186621759971	0.0
18	1.5558987753002106E-4	0.0	0.0	14.32324108236617	0.0
19	1.5558987753002106E-4	0.0	0.0	14.907740388953949	0.0
20	1.5558987753002106E-4	0.0	0.0	15.51412600498095	0.0
21	2.0745317004002809E-4	0.0	0.0	16.24674687497731	0.0
22	2.0745317004002809E-4	0.0	0.0	17.027911786763035	0.0
23	2.0745317004002809E-4	0.0	0.0	17.78070747754579	0.0
24	2.0745317004002809E-4	0.0	0.0	18.39679152927216	0.0
25	2.0745317004002809E-4	0.0	0.0	18.935080642233522	0.0
26	2.0745317004002809E-4	0.0	0.0	19.42544807291564	0.0
27	2.0745317004002809E-4	0.0	0.0	19.93070026854813	0.0
28	2.0745317004002809E-4	0.0	0.0	20.45306735070892	0.0
29	2.0745317004002809E-4	0.0	0.0	20.995712980241123	0.0
30	2.0745317004002809E-4	0.0	0.0	21.606662566009007	0.0
31	2.0745317004002809E-4	0.0	0.0	22.15018987151388	0.0
32	2.0745317004002809E-4	0.0	0.0	22.704504741860834	0.0
33	2.0745317004002809E-4	0.0	0.0	23.221478041600584	0.0
34	2.0745317004002809E-4	0.0	0.0	23.743533944006316	0.0
35	2.0745317004002809E-4	0.0	0.0	24.29815999410833	0.0
36	2.0745317004002809E-4	0.0	0.0	24.834322712076784	0.0
37	2.0745317004002809E-4	0.0	0.0	25.364676741284114	0.0
38	2.0745317004002809E-4	0.0	0.0	25.887251276614943	0.0
39	2.0745317004002809E-4	0.0	0.0	26.43005249602468	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGG	60	3.6379788E-12	41.249996	2
TCGTTAG	50	1.0822987E-9	40.500004	1
CTACGAA	135	0.0	40.0	11
CGAATAT	125	0.0	39.600002	14
CTAACGG	145	0.0	38.793106	2
TCGATAG	65	9.094947E-12	38.07692	1
CGTAAGG	290	0.0	38.017242	2
CGTTAGG	240	0.0	37.499996	2
CGATACG	60	1.5643309E-10	37.499996	10
GTACGAG	185	0.0	36.486485	1
CGTTTTT	2905	0.0	36.3253	1
ATAGGGC	815	0.0	36.165646	4
ATAGCGG	280	0.0	36.160717	2
ATCGCTC	25	0.0021074614	36.000004	30
TACGTTG	50	4.8818038E-8	36.000004	1
ACGTAGG	195	0.0	35.76923	2
TATAGCG	120	0.0	35.625	1
ATAACGG	95	0.0	35.526314	2
GAATGCG	115	0.0	35.21739	1
TAAGGGA	1165	0.0	35.15021	4
>>END_MODULE