##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550013_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	795049
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20848148982012	33.0	31.0	34.0	30.0	34.0
2	32.36009227104241	34.0	31.0	34.0	30.0	34.0
3	32.3695080429005	34.0	31.0	34.0	30.0	34.0
4	35.93198783974321	37.0	35.0	37.0	35.0	37.0
5	35.80424728538744	37.0	35.0	37.0	35.0	37.0
6	35.75485284554789	37.0	35.0	37.0	35.0	37.0
7	35.98196589141047	37.0	35.0	37.0	35.0	37.0
8	35.92817801166972	37.0	35.0	37.0	35.0	37.0
9	37.41428012613059	39.0	37.0	39.0	35.0	39.0
10	37.31523465849275	39.0	37.0	39.0	34.0	39.0
11	37.20967890029419	39.0	37.0	39.0	34.0	39.0
12	37.04993780257569	39.0	37.0	39.0	33.0	39.0
13	36.91817737019982	39.0	37.0	39.0	33.0	39.0
14	37.9872674514401	40.0	37.0	41.0	33.0	41.0
15	38.08708771409058	40.0	37.0	41.0	33.0	41.0
16	38.12635824961732	40.0	37.0	41.0	33.0	41.0
17	38.13860152015788	40.0	37.0	41.0	34.0	41.0
18	38.10046676368375	40.0	37.0	41.0	34.0	41.0
19	38.057518467415214	40.0	37.0	41.0	34.0	41.0
20	38.01664928828286	40.0	36.0	41.0	34.0	41.0
21	37.99977737221228	40.0	36.0	41.0	34.0	41.0
22	37.89459643367893	40.0	36.0	41.0	33.0	41.0
23	37.642371728031854	39.0	35.0	41.0	33.0	41.0
24	37.43667119888208	39.0	35.0	41.0	32.0	41.0
25	37.43169917828964	39.0	35.0	41.0	33.0	41.0
26	37.51635182234051	39.0	35.0	41.0	33.0	41.0
27	37.615887825781805	39.0	35.0	41.0	33.0	41.0
28	37.60257292317832	39.0	36.0	41.0	33.0	41.0
29	37.54313130385675	39.0	35.0	41.0	33.0	41.0
30	37.529655404886995	39.0	36.0	41.0	33.0	41.0
31	37.38910180378819	39.0	35.0	41.0	33.0	41.0
32	37.23616154476014	39.0	35.0	41.0	32.0	41.0
33	37.211954231751754	39.0	35.0	41.0	32.0	41.0
34	37.13851221748597	39.0	35.0	41.0	32.0	41.0
35	37.0419860914233	39.0	35.0	41.0	32.0	41.0
36	37.01605812975049	39.0	35.0	41.0	32.0	41.0
37	36.95312364395151	39.0	35.0	41.0	32.0	41.0
38	36.83210468788716	39.0	35.0	41.0	31.0	41.0
39	36.789070862299056	39.0	35.0	41.0	31.0	41.0
40	36.74139707112392	39.0	35.0	41.0	31.0	41.0
41	36.68902545629263	39.0	35.0	41.0	31.0	41.0
42	36.62004606005416	39.0	35.0	41.0	31.0	41.0
43	36.51530660374392	39.0	35.0	41.0	31.0	41.0
44	36.38887917600047	39.0	35.0	40.0	31.0	41.0
45	36.33708991521277	38.0	35.0	40.0	31.0	41.0
46	36.25229136820498	38.0	35.0	40.0	31.0	41.0
47	36.13716890405497	38.0	35.0	40.0	30.0	41.0
48	36.0725842055018	38.0	35.0	40.0	30.0	41.0
49	36.055938690571274	38.0	35.0	40.0	30.0	41.0
50	35.94751895795102	38.0	35.0	40.0	30.0	41.0
51	34.94351668890848	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	6.0
11	9.0
12	10.0
13	10.0
14	19.0
15	31.0
16	64.0
17	84.0
18	203.0
19	325.0
20	653.0
21	1077.0
22	1623.0
23	2275.0
24	3232.0
25	4181.0
26	5384.0
27	6508.0
28	7639.0
29	9342.0
30	11971.0
31	15182.0
32	20209.0
33	28857.0
34	54633.0
35	69831.0
36	63899.0
37	97590.0
38	168114.0
39	221940.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.092625737533155	22.128070093792964	24.294854782535417	11.484449386138465
2	31.909857128302782	25.98217216800474	24.50930697353245	17.598663730160027
3	31.917655389793588	24.671435345494427	26.05562676011164	17.355282504600346
4	29.961423761302765	26.030345299472106	25.423841800945603	18.584389138279526
5	24.353341743716424	30.876335923949345	24.335229652511984	20.43509267982225
6	26.015629225368496	33.988596929245865	24.80136444420407	15.194409401181563
7	76.56056419164102	7.170124105558273	9.995484555040003	6.273827147760704
8	75.02191688814148	7.0811987688809115	10.644375378121348	7.252508964856254
9	68.16598725361581	9.11428100657947	13.8688307261565	8.850901013648215
10	40.283177514845	23.507859264020205	20.07524064554512	16.133722575589683
11	30.558871214227047	24.046065085296632	24.330701629710873	21.064362070765448
12	27.911109881277756	21.21554772095808	30.548934719746835	20.32440767801733
13	24.353970635772136	24.06480606855678	31.383222920851416	20.198000374819667
14	18.944995843023513	29.16021528232851	29.538556743043507	22.356232131604468
15	18.364402697192247	25.758663931405483	35.213930210590796	20.66300316081147
16	20.799346958489352	24.93720512823738	31.314170573134486	22.949277340138785
17	19.88619569359876	25.917018951033207	27.73300765110075	26.463777704267283
18	21.374154297408086	26.601127729234296	29.972114926249827	22.052603047107787
19	22.42780004754424	27.192286261601485	27.67716203655372	22.702751654300553
20	24.339380340079668	27.26133860931842	27.43503859510546	20.964242455496453
21	22.77721247369659	28.05097547446761	28.54880642576747	20.62300562606833
22	20.80312031082361	25.77048710205283	26.8442573979717	26.582135189151863
23	21.358683552837622	25.8522430692951	28.042045207276534	24.747028170590742
24	23.531757162137175	24.585528690684473	27.712631548495754	24.170082598682598
25	21.15177806650911	26.493964522941354	25.923056314768022	26.43120109578152
26	19.326733320839345	28.24907647201619	27.02927744076151	25.394912766382955
27	21.604831903442427	26.865513949454687	26.832685784146637	24.696968362956245
28	19.965184535795906	26.883122927014565	27.663326411328104	25.488366125861422
29	20.499742783149212	27.83551705618144	26.76174676026257	24.902993400406768
30	22.323907079940987	26.0671983739367	26.37321724824508	25.235677297877242
31	23.876641565488416	27.731498310167048	23.11832352471357	25.273536599630965
32	22.92537944202181	29.341965086428633	24.262026617227367	23.470628854322186
33	23.243976157444386	26.06845615804812	26.39334179402779	24.294225890479705
34	21.441697304191315	25.96280229268888	27.43252302688262	25.16297737623719
35	20.77997708317349	26.420258374012167	27.183984886466117	25.615779656348224
36	23.76884946713976	26.58263830279643	26.268443831763825	23.38006839829998
37	23.168886445992637	26.924252467457983	26.718730543652025	23.188130542897355
38	22.099392616052597	28.16618849907364	26.628295866040965	23.106123018832804
39	24.033361465771293	24.516853678200967	27.99387207580916	23.455912780218576
40	22.120523389124443	23.24888151547892	29.95438017027881	24.676214925117822
41	19.8965095233124	24.861486524729923	27.61137992752648	27.6306240244312
42	23.010405647953775	24.400760204716942	27.33932122422643	25.249512923102852
43	24.756209994604106	22.87519385597617	27.587859364642934	24.78073678477679
44	22.46528201406454	23.838027593267835	27.001103076665718	26.695587316001905
45	21.691619007130377	23.65061776066632	26.71369940720635	27.944063824996952
46	22.950157789016778	23.704828255868506	28.607544943770762	24.737469011343954
47	20.502258351372056	23.29265240255632	30.86727987834712	25.337809367724507
48	20.713943417323964	22.11197045716679	30.81948408211318	26.35460204339607
49	22.949780453783355	20.664386723334033	31.397058546077034	24.988774276805582
50	20.577599619646083	20.900095465814054	31.326245300604118	27.19605961393575
51	20.17573759604754	20.53948876107007	28.294230921616155	30.990542721266234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	760.0
1	1311.0
2	1862.0
3	2397.5
4	2933.0
5	2197.5
6	1462.0
7	1466.0
8	1470.0
9	1536.0
10	1602.0
11	1749.0
12	1896.0
13	2041.0
14	2186.0
15	2174.5
16	2163.0
17	2147.5
18	2132.0
19	2054.0
20	1976.0
21	2169.5
22	2363.0
23	2769.0
24	3175.0
25	3700.0
26	5063.5
27	5902.0
28	7355.5
29	8809.0
30	9790.5
31	10772.0
32	11882.0
33	12992.0
34	14401.0
35	15810.0
36	17008.0
37	18206.0
38	18972.0
39	19738.0
40	24136.0
41	28534.0
42	34095.0
43	39656.0
44	46421.0
45	53186.0
46	64781.0
47	76376.0
48	86908.0
49	97440.0
50	93708.0
51	89976.0
52	73905.5
53	57835.0
54	50664.5
55	43494.0
56	39974.5
57	36455.0
58	34942.5
59	33430.0
60	32418.5
61	31407.0
62	29078.0
63	26749.0
64	23401.5
65	20054.0
66	16965.0
67	13876.0
68	11440.0
69	9004.0
70	7410.0
71	5816.0
72	4799.5
73	3783.0
74	3062.5
75	2063.0
76	1784.0
77	1291.5
78	799.0
79	610.5
80	422.0
81	266.5
82	111.0
83	72.5
84	34.0
85	33.0
86	32.0
87	21.0
88	10.0
89	9.0
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	795049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.237908555188174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.99955254065179	20.17402607532479
2	12.689820310363034	7.42047611632328
3	5.024883831474749	4.407512818953068
4	2.5836918981306183	3.021669898092943
5	1.4739001966137186	2.1546879584032888
6	1.011747689829682	1.7748831861697871
7	0.7352881145028217	1.5048800657485162
8	0.5533035651852292	1.2941951233716265
9	0.4411228450465654	1.160775846456833
>10	6.013146606023638	41.69573191122544
>50	0.38127127247214315	6.838960661677031
>100	0.08182534143744699	4.6119908438208626
>500	0.0069638588457401705	1.383139133642982
>1k	0.0034819294228700852	2.5570703607895924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	4583	0.5764424582635788	No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT	4445	0.5590850375259889	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	4271	0.5371995939872888	No Hit
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	1829	0.2300487139786353	No Hit
ACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	1520	0.19118318493577124	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1276	0.1604932526171343	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC	1148	0.14439361599096406	No Hit
AGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	1022	0.12854553618707776	No Hit
CGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	980	0.12326284291911567	No Hit
GCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	978	0.12301128609683176	No Hit
GGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	898	0.11294901320547537	No Hit
ACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	889	0.1118170075051978	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	818	0.10288674031411901	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2577841114195477E-4	0.0	0.0	0.5920389812451811	0.0
2	1.2577841114195477E-4	0.0	0.0	2.227032547679451	0.0
3	1.2577841114195477E-4	0.0	0.0	3.206343256830711	0.0
4	1.2577841114195477E-4	0.0	0.0	4.237852006605882	0.0
5	1.2577841114195477E-4	0.0	0.0	6.6553130687542525	0.0
6	1.2577841114195477E-4	0.0	0.0	8.00894032946397	0.0
7	1.2577841114195477E-4	0.0	0.0	9.42067721612127	0.0
8	1.2577841114195477E-4	0.0	0.0	11.537905210873795	0.0
9	1.2577841114195477E-4	0.0	0.0	12.306285524539996	0.0
10	1.2577841114195477E-4	0.0	0.0	13.847071061028943	0.0
11	1.2577841114195477E-4	0.0	0.0	16.155859575950664	0.0
12	1.2577841114195477E-4	0.0	0.0	17.973609173774197	0.0
13	1.2577841114195477E-4	0.0	0.0	18.71519868586716	0.0
14	1.2577841114195477E-4	0.0	0.0	19.03178294671146	0.0
15	1.2577841114195477E-4	0.0	0.0	19.614262768709853	0.0
16	1.2577841114195477E-4	0.0	0.0	20.764129003369604	0.0
17	1.2577841114195477E-4	0.0	0.0	22.086437439704973	0.0
18	1.2577841114195477E-4	0.0	0.0	23.712500738948165	0.0
19	1.2577841114195477E-4	0.0	0.0	24.605527458056045	0.0
20	1.2577841114195477E-4	0.0	0.0	25.46107221064362	0.0
21	2.5155682228390953E-4	0.0	0.0	26.48352491481657	0.0
22	2.5155682228390953E-4	0.0	0.0	27.48711085731823	0.0
23	2.5155682228390953E-4	0.0	0.0	28.492583475987015	0.0
24	2.5155682228390953E-4	0.0	0.0	29.26008334077522	0.0
25	2.5155682228390953E-4	0.0	0.0	29.926708919827583	0.0
26	2.5155682228390953E-4	0.0	0.0	30.511704310048813	0.0
27	3.773352334258643E-4	0.0	0.0	31.09632236503662	0.0
28	5.031136445678191E-4	0.0	0.0	31.715655261499606	0.0
29	5.031136445678191E-4	0.0	0.0	32.350710459355334	0.0
30	5.031136445678191E-4	0.0	0.0	33.05003842530461	0.0
31	5.031136445678191E-4	0.0	0.0	33.67855314578095	0.0
32	5.031136445678191E-4	0.0	0.0	34.30090472411134	0.0
33	5.031136445678191E-4	0.0	0.0	34.89973573955819	0.0
34	5.031136445678191E-4	0.0	0.0	35.483850680901426	0.0
35	5.031136445678191E-4	0.0	0.0	36.06155092327643	0.0
36	5.031136445678191E-4	0.0	0.0	36.641640955463124	0.0
37	6.288920557097739E-4	0.0	0.0	37.25204358473503	0.0
38	6.288920557097739E-4	0.0	0.0	37.8419443329908	0.0
39	6.288920557097739E-4	0.0	0.0	38.46505058178804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTACA	30	2.1646538E-6	45.000004	34
TCGACAT	35	1.2113014E-7	45.000004	1
TGATCGG	25	3.8897168E-5	45.0	2
CTTGTCA	40	6.8102963E-9	45.0	11
AATCCGG	20	7.0319726E-4	45.0	2
ATTAGCG	40	6.8102963E-9	45.0	1
ACGTTGC	20	7.0319726E-4	45.0	27
GTTTACG	20	7.0319726E-4	45.0	1
CGGTTAT	20	7.0319726E-4	45.0	6
GACCGCA	25	3.8897168E-5	45.0	28
ACGATTG	20	7.0319726E-4	45.0	1
AATGCGA	75	0.0	45.0	25
TACGCGC	25	3.8897168E-5	45.0	34
CGAATAT	155	0.0	45.0	14
GATGCGA	20	7.0319726E-4	45.0	11
ATAACGG	25	3.8897168E-5	45.0	2
ACGTAAG	45	3.8380676E-10	45.0	1
AATAGTG	20	7.0319726E-4	45.0	19
CGCGTAA	25	3.8897168E-5	45.0	31
TCGACTC	20	7.0319726E-4	45.0	25
>>END_MODULE