FastQCFastQC Report
Sat 18 Jun 2016
SRR3550012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506260
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC49930.33148327645957537TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT47760.31707673310052714TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC37850.25128463877418244TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31610.2098575279168271No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCC20950.13908621353551182No Hit
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC18620.1236174365647365TruSeq Adapter, Index 13 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG406.8175723E-945.0000042
TAGTACG551.8189894E-1245.0000041
TCGATTG302.1659525E-644.9999961
TATAGCG603.6379788E-1241.2499961
CGCATCG556.184564E-1140.90909221
TGCGTAG1250.039.61
GGCGAAC403.4591721E-739.37500410
AATGCGA750.039.025
TATACGG700.038.571432
CGTCATA700.038.5714338
TATCGCG659.094947E-1238.076921
AGTACGG1850.037.70272
TCATACG301.1400902E-437.49999631
ATAGCGG2200.036.8181842
ACGTGCG552.750312E-936.8181841
TATGGGT1850.036.4864854
CGAACAC250.002107316236.06
CCGTGTA504.8807124E-836.020
ATGCGAA751.8189894E-1236.026
CACCCGT250.002107316236.038
Produced by FastQC (version 0.11.5)