##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550012_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1506260 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29611753614914 33.0 31.0 34.0 30.0 34.0 2 32.44871735291384 34.0 31.0 34.0 31.0 34.0 3 32.456234647404834 34.0 31.0 34.0 30.0 34.0 4 35.99781246265585 37.0 35.0 37.0 35.0 37.0 5 35.87779599803487 37.0 35.0 37.0 35.0 37.0 6 35.828013092029266 37.0 35.0 37.0 35.0 37.0 7 36.03757452232682 37.0 35.0 37.0 35.0 37.0 8 35.99557446921514 37.0 35.0 37.0 35.0 37.0 9 37.50478005125277 39.0 38.0 39.0 35.0 39.0 10 37.32214026794843 39.0 37.0 39.0 34.0 39.0 11 37.24974771951722 39.0 37.0 39.0 34.0 39.0 12 37.16970576128955 39.0 37.0 39.0 34.0 39.0 13 37.14366775988209 39.0 37.0 39.0 33.0 39.0 14 38.30420843679046 40.0 38.0 41.0 34.0 41.0 15 38.39390742634074 40.0 38.0 41.0 34.0 41.0 16 38.40146521848818 40.0 38.0 41.0 34.0 41.0 17 38.41550661904319 40.0 38.0 41.0 34.0 41.0 18 38.38001938576341 40.0 38.0 41.0 34.0 41.0 19 38.33713834265001 40.0 37.0 41.0 34.0 41.0 20 38.29612749458925 40.0 37.0 41.0 34.0 41.0 21 38.275229376070534 40.0 37.0 41.0 34.0 41.0 22 38.18490433258535 40.0 37.0 41.0 34.0 41.0 23 37.91527558323264 40.0 37.0 41.0 33.0 41.0 24 37.67755102040816 40.0 36.0 41.0 33.0 41.0 25 37.66667042874404 40.0 36.0 41.0 33.0 41.0 26 37.758450068381286 40.0 36.0 41.0 33.0 41.0 27 37.80701339742143 40.0 36.0 41.0 33.0 41.0 28 37.78637353444957 40.0 36.0 41.0 33.0 41.0 29 37.77392747600016 40.0 36.0 41.0 33.0 41.0 30 37.671074050960655 40.0 36.0 41.0 33.0 41.0 31 37.56595408495213 40.0 36.0 41.0 33.0 41.0 32 37.4565672593045 40.0 36.0 41.0 33.0 41.0 33 37.38882463850862 39.0 36.0 41.0 33.0 41.0 34 37.35849521330979 39.0 36.0 41.0 33.0 41.0 35 37.29075657588995 39.0 35.0 41.0 32.0 41.0 36 37.23852190192928 39.0 35.0 41.0 32.0 41.0 37 37.20339582807749 39.0 35.0 41.0 32.0 41.0 38 37.08134253050602 39.0 35.0 41.0 32.0 41.0 39 37.02137280416395 39.0 35.0 41.0 32.0 41.0 40 36.97512049712533 39.0 35.0 41.0 32.0 41.0 41 36.909082097380264 39.0 35.0 41.0 31.0 41.0 42 36.86130880458885 39.0 35.0 41.0 31.0 41.0 43 36.7702627700397 39.0 35.0 41.0 31.0 41.0 44 36.67407884428983 39.0 35.0 41.0 31.0 41.0 45 36.591499475522156 39.0 35.0 41.0 31.0 41.0 46 36.49009599936266 39.0 35.0 40.0 31.0 41.0 47 36.38392840545457 38.0 35.0 40.0 31.0 41.0 48 36.31076640155086 38.0 35.0 40.0 31.0 41.0 49 36.29006479625031 38.0 35.0 40.0 31.0 41.0 50 36.21259742673907 38.0 35.0 40.0 31.0 41.0 51 35.25856824187059 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 8.0 9 12.0 10 23.0 11 10.0 12 27.0 13 19.0 14 29.0 15 45.0 16 80.0 17 157.0 18 283.0 19 528.0 20 938.0 21 1613.0 22 2343.0 23 3546.0 24 5025.0 25 6859.0 26 9157.0 27 11583.0 28 13952.0 29 17258.0 30 21298.0 31 27303.0 32 35909.0 33 50322.0 34 88774.0 35 114365.0 36 122065.0 37 188504.0 38 327824.0 39 456066.0 40 335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35466652503552 21.296456123112876 23.901384887071288 11.447492464780316 2 32.428664374012456 24.14921726660736 25.159534210561258 18.262584148818927 3 32.644496965995245 24.05361624155192 25.8182518290335 17.48363496341933 4 29.59615205874152 25.54426194680865 25.570884176702563 19.288701817747267 5 25.39820482519618 29.816432753973416 24.28677651932601 20.498585901504388 6 26.919788084394458 34.52684131557633 23.571030233824175 14.982340366205039 7 79.1275078671677 6.787938337338839 8.691460969553729 5.393092825939745 8 78.99041334165416 5.870898782414723 9.14291025453773 5.995777621393385 9 72.0931977215089 8.140427283470316 11.933729900548379 7.832645094472401 10 38.53849932946503 25.071501599989375 19.396916866941964 16.993082203603628 11 28.51924634525248 24.667321710727233 25.218222617609175 21.595209326411112 12 25.50934101682312 22.439485878931926 30.785787314275094 21.26538578996986 13 23.821850145393224 23.771062100832527 32.04626027379071 20.360827479983534 14 20.472893126020743 26.95889155922616 30.03724456601118 22.530970748741915 15 19.854739553596325 25.17420631232324 33.32897374955187 21.642080384528565 16 21.818012826470863 25.100580245110404 30.17394075392031 22.907466174498424 17 21.310132380863863 25.10051385550967 28.621419940780473 24.96793382284599 18 22.578040975661573 24.97848976936253 29.79538725054108 22.648082004434826 19 22.705907346673218 26.163544142445527 27.814255175069373 23.316293335811878 20 23.81268838049208 26.32042276897747 27.857142857142858 22.009745993387593 21 23.44681529085284 26.4500816592089 28.48153705203617 21.62156599790209 22 22.057878453918978 24.770823098269886 26.686959754624034 26.484338693187098 23 21.841249186727392 25.753123630714487 27.844528833003597 24.561098349554523 24 22.243503777568282 25.18575810285077 28.714896498612458 23.85584162096849 25 22.093861617516232 26.325733937036105 26.478098070718204 25.102306374729462 26 20.997370971810973 26.592553742381792 27.203935575531446 25.20613971027578 27 21.870726169452816 26.47710222670721 27.51258082933889 24.139590774501084 28 19.69095640858816 26.397567485029143 28.5503166783955 25.3611594279872 29 20.850185226986046 26.199394526841314 26.847224250793357 26.103195995379284 30 22.20393557553145 27.72535949968797 26.381169253648107 23.689535671132475 31 21.93917384780848 28.025174936597928 25.367532829657563 24.668118385936026 32 21.437733193472575 28.298434533214717 24.435422835367067 25.828409437945638 33 20.697555534901014 28.824771287825474 24.8419927502556 25.63568042701791 34 19.50566303294252 26.121519525181576 27.092799383904502 27.2800180579714 35 19.89390941802876 26.574296602180237 25.112795931645266 28.41899804814574 36 20.14784964083226 28.999375937753108 24.907585675779746 25.945188745634884 37 19.830839297332467 28.169107590986947 26.9694475057427 25.030605605937883 38 19.486210879927768 29.12485228313837 25.72563833601105 25.663298500922814 39 20.98283164925046 26.2584148818929 26.296588902314344 26.462164566542295 40 20.890085377026544 24.78058236957763 27.86351625881322 26.46581599458261 41 18.991077237661493 25.33991475575266 25.73314036089387 29.935867645691978 42 19.83249903735079 25.39050363151116 26.7777807284267 27.999216602711353 43 21.019213150452114 24.578492424946557 28.279845445009492 26.122448979591837 44 20.248629054744864 25.73851791855324 27.160583166252838 26.852269860449056 45 21.026914344137136 24.765379151009785 26.871456455060876 27.3362500497922 46 21.713781153320145 25.31654561629466 26.50664559903337 26.463027631351828 47 19.251191693333155 25.104165283549985 28.909683587162903 26.734959435953954 48 18.653486117934488 24.750242322042677 28.8995923678515 27.696679192171338 49 20.60062671783092 23.935177193844357 29.418294318377967 26.045901769946756 50 19.310543996388404 23.22009480434985 29.570525672858604 27.898835526403143 51 19.549812117429926 23.311247726156175 26.862626638163402 30.276313518250504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 698.0 1 1563.5 2 2429.0 3 4083.0 4 5737.0 5 4091.5 6 2446.0 7 2308.0 8 2170.0 9 2223.0 10 2276.0 11 2405.5 12 2535.0 13 2580.0 14 2625.0 15 2673.5 16 2722.0 17 2731.5 18 2741.0 19 3002.0 20 3263.0 21 3739.5 22 4216.0 23 4768.5 24 5321.0 25 7135.0 26 10152.0 27 11355.0 28 13993.5 29 16632.0 30 19468.0 31 22304.0 32 25752.5 33 29201.0 34 31233.5 35 33266.0 36 37174.5 37 41083.0 38 44715.5 39 48348.0 40 55483.5 41 62619.0 42 70739.0 43 78859.0 44 87835.0 45 96811.0 46 115448.5 47 134086.0 48 150714.0 49 167342.0 50 160967.0 51 154592.0 52 133241.5 53 111891.0 54 97854.5 55 83818.0 56 77649.5 57 71481.0 58 67633.0 59 63785.0 60 60328.5 61 56872.0 62 53814.5 63 50757.0 64 44598.5 65 38440.0 66 32833.5 67 27227.0 68 23768.0 69 20309.0 70 17199.5 71 14090.0 72 11717.0 73 9344.0 74 7631.0 75 4799.0 76 3680.0 77 2942.0 78 2204.0 79 1593.0 80 982.0 81 741.5 82 501.0 83 358.0 84 215.0 85 145.5 86 76.0 87 47.0 88 18.0 89 13.5 90 9.0 91 8.5 92 8.0 93 7.0 94 6.0 95 4.5 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1506260.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.100854419939367 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6678589301296 21.27162933824643 2 11.8918609545671 7.159103507511709 3 4.484173403815071 4.049323524660044 4 2.157324407442782 2.5974923170006856 5 1.273989038025846 1.9174079283107295 6 0.8631016955783435 1.5588059092923925 7 0.6579362843216239 1.3863111018372725 8 0.5035109448112207 1.2124887718886945 9 0.4555332072275784 1.2340744878784866 >10 6.784373912119143 46.83166727101632 >50 0.18559729686378176 3.5764805510968003 >100 0.06489394859326864 3.735773312644345 >500 0.006041849218744356 1.1764633377049936 >1k 0.0038041272858760763 2.292978640911107 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC 4993 0.33148327645957537 TruSeq Adapter, Index 13 (95% over 23bp) CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT 4776 0.31707673310052714 TruSeq Adapter, Index 16 (95% over 22bp) CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC 3785 0.25128463877418244 TruSeq Adapter, Index 13 (95% over 23bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3161 0.2098575279168271 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCC 2095 0.13908621353551182 No Hit TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC 1862 0.1236174365647365 TruSeq Adapter, Index 13 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.6555840293176477E-4 0.0 0.0 0.3437653525951695 0.0 2 2.6555840293176477E-4 0.0 0.0 1.2185147318524026 0.0 3 2.6555840293176477E-4 0.0 0.0 1.8206020208994462 0.0 4 2.6555840293176477E-4 0.0 0.0 2.5123816605366933 0.0 5 2.6555840293176477E-4 0.0 0.0 4.16933331562944 0.0 6 2.6555840293176477E-4 0.0 0.0 5.220878201638495 0.0 7 2.6555840293176477E-4 0.0 0.0 6.277867034907652 0.0 8 2.6555840293176477E-4 0.0 0.0 8.00412943316559 0.0 9 2.6555840293176477E-4 0.0 0.0 8.61225817587933 0.0 10 2.6555840293176477E-4 0.0 0.0 9.845776957497378 0.0 11 2.6555840293176477E-4 0.0 0.0 11.667839549612948 0.0 12 2.6555840293176477E-4 0.0 0.0 13.01116673084328 0.0 13 2.6555840293176477E-4 0.0 6.638960073294119E-5 13.582250076348041 0.0 14 2.6555840293176477E-4 0.0 6.638960073294119E-5 13.809036952451768 0.0 15 2.6555840293176477E-4 0.0 6.638960073294119E-5 14.284851220904757 0.0 16 2.6555840293176477E-4 0.0 6.638960073294119E-5 15.191268439711603 0.0 17 3.3194800366470595E-4 0.0 6.638960073294119E-5 16.25887960909803 0.0 18 3.3194800366470595E-4 0.0 6.638960073294119E-5 17.424149881162613 0.0 19 3.3194800366470595E-4 0.0 6.638960073294119E-5 18.112078923957352 0.0 20 3.3194800366470595E-4 0.0 6.638960073294119E-5 18.81965928856904 0.0 21 3.3194800366470595E-4 0.0 6.638960073294119E-5 19.686375526137585 0.0 22 3.3194800366470595E-4 0.0 6.638960073294119E-5 20.57022028069523 0.0 23 3.3194800366470595E-4 0.0 6.638960073294119E-5 21.43740124546891 0.0 24 3.3194800366470595E-4 0.0 6.638960073294119E-5 22.143321870062273 0.0 25 3.3194800366470595E-4 0.0 6.638960073294119E-5 22.77594837544647 0.0 26 3.3194800366470595E-4 0.0 6.638960073294119E-5 23.335745488826632 0.0 27 3.3194800366470595E-4 0.0 6.638960073294119E-5 23.901318497470555 0.0 28 3.9833760439764713E-4 0.0 6.638960073294119E-5 24.493779294411322 0.0 29 3.9833760439764713E-4 0.0 6.638960073294119E-5 25.12527717658306 0.0 30 3.9833760439764713E-4 0.0 6.638960073294119E-5 25.788177339901477 0.0 31 4.6472720513058836E-4 0.0 6.638960073294119E-5 26.39882888744307 0.0 32 4.6472720513058836E-4 0.0 6.638960073294119E-5 27.00011950128132 0.0 33 4.6472720513058836E-4 0.0 6.638960073294119E-5 27.58049739088869 0.0 34 4.6472720513058836E-4 0.0 6.638960073294119E-5 28.15556411243743 0.0 35 4.6472720513058836E-4 0.0 6.638960073294119E-5 28.76734428319148 0.0 36 4.6472720513058836E-4 0.0 6.638960073294119E-5 29.333780356644937 0.0 37 4.6472720513058836E-4 0.0 6.638960073294119E-5 29.8923824572119 0.0 38 4.6472720513058836E-4 0.0 6.638960073294119E-5 30.441424455273324 0.0 39 4.6472720513058836E-4 0.0 6.638960073294119E-5 31.01662395602353 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAACGG 40 6.8175723E-9 45.000004 2 TAGTACG 55 1.8189894E-12 45.000004 1 TCGATTG 30 2.1659525E-6 44.999996 1 TATAGCG 60 3.6379788E-12 41.249996 1 CGCATCG 55 6.184564E-11 40.909092 21 TGCGTAG 125 0.0 39.6 1 GGCGAAC 40 3.4591721E-7 39.375004 10 AATGCGA 75 0.0 39.0 25 TATACGG 70 0.0 38.57143 2 CGTCATA 70 0.0 38.57143 38 TATCGCG 65 9.094947E-12 38.07692 1 AGTACGG 185 0.0 37.7027 2 TCATACG 30 1.1400902E-4 37.499996 31 ATAGCGG 220 0.0 36.818184 2 ACGTGCG 55 2.750312E-9 36.818184 1 TATGGGT 185 0.0 36.486485 4 CGAACAC 25 0.0021073162 36.0 6 CCGTGTA 50 4.8807124E-8 36.0 20 ATGCGAA 75 1.8189894E-12 36.0 26 CACCCGT 25 0.0021073162 36.0 38 >>END_MODULE