FastQCFastQC Report
Sat 18 Jun 2016
SRR3550008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616691
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT70320.4349625253063202No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC67380.4167772320127965No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC57840.35776781091748516No Hit
TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC28050.17350254315759783No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC26320.16280167329440196No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.15581208777682315No Hit
ACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC20320.12568882983823132No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.0341385E-445.01
CGTCTAA207.0341385E-445.018
TCGAACG253.8915125E-545.01
CGGTCTA1200.041.24999631
CGACGGT1300.039.8076928
TACGGGT850.039.7058834
CTAACGG850.039.7058832
CGTATCC356.249864E-638.5714343
AATGCGA700.038.5714325
ACGTATC356.249864E-638.5714342
GTACGAG1350.038.3333361
ACGTAAG1000.038.251
TAGTGCG1000.038.251
CGTTTTT21050.037.731591
CGAGGGT2450.037.653064
CGTCGTG301.1401275E-437.49999616
GCCCGAT301.1401275E-437.4999968
CACGACG1350.036.66666826
TATGGGA10050.036.492544
TAGGGAT10300.036.4805835
Produced by FastQC (version 0.11.5)