##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550008_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1616691 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.247698539795174 33.0 31.0 34.0 30.0 34.0 2 32.40820602081659 34.0 31.0 34.0 30.0 34.0 3 32.40491349305464 34.0 31.0 34.0 30.0 34.0 4 35.958693405233284 37.0 35.0 37.0 35.0 37.0 5 35.84515037196347 37.0 35.0 37.0 35.0 37.0 6 35.790277795818746 37.0 35.0 37.0 35.0 37.0 7 36.037029958105784 37.0 35.0 37.0 35.0 37.0 8 36.00586321071869 37.0 35.0 37.0 35.0 37.0 9 37.53881972498146 39.0 38.0 39.0 35.0 39.0 10 37.31546411775658 39.0 37.0 39.0 34.0 39.0 11 37.2334131878015 39.0 37.0 39.0 34.0 39.0 12 37.10984226422984 39.0 37.0 39.0 34.0 39.0 13 37.05527153921189 39.0 37.0 39.0 33.0 39.0 14 38.16042521421843 40.0 37.0 41.0 33.0 41.0 15 38.23589418138655 40.0 37.0 41.0 33.0 41.0 16 38.24356416903416 40.0 37.0 41.0 34.0 41.0 17 38.23276927996754 40.0 37.0 41.0 34.0 41.0 18 38.206291740351126 40.0 37.0 41.0 34.0 41.0 19 38.17488808931329 40.0 37.0 41.0 34.0 41.0 20 38.15025629511143 40.0 37.0 41.0 34.0 41.0 21 38.134513645464715 40.0 37.0 41.0 34.0 41.0 22 38.04978254966472 40.0 37.0 41.0 34.0 41.0 23 37.78446283179655 40.0 36.0 41.0 33.0 41.0 24 37.58445677003212 39.0 36.0 41.0 33.0 41.0 25 37.60019756403666 39.0 36.0 41.0 33.0 41.0 26 37.70864067406821 40.0 36.0 41.0 33.0 41.0 27 37.800090431628554 40.0 36.0 41.0 33.0 41.0 28 37.767971121259414 40.0 36.0 41.0 33.0 41.0 29 37.74225996186037 40.0 36.0 41.0 33.0 41.0 30 37.6812551068819 40.0 36.0 41.0 33.0 41.0 31 37.5302454210483 40.0 36.0 41.0 33.0 41.0 32 37.38882321977422 39.0 35.0 41.0 33.0 41.0 33 37.376126916027864 39.0 35.0 41.0 33.0 41.0 34 37.25830600900234 39.0 35.0 41.0 32.0 41.0 35 37.178382263524696 39.0 35.0 41.0 32.0 41.0 36 37.16224683628473 39.0 35.0 41.0 32.0 41.0 37 37.10754250503034 39.0 35.0 41.0 32.0 41.0 38 36.96524072936634 39.0 35.0 41.0 31.0 41.0 39 36.92138077096984 39.0 35.0 41.0 31.0 41.0 40 36.8630962874167 39.0 35.0 41.0 31.0 41.0 41 36.79990239322171 39.0 35.0 41.0 31.0 41.0 42 36.793031568803194 39.0 35.0 41.0 31.0 41.0 43 36.75109838552945 39.0 35.0 41.0 31.0 41.0 44 36.646184706910596 39.0 35.0 41.0 31.0 41.0 45 36.54736557573463 39.0 35.0 40.0 31.0 41.0 46 36.41853699934001 38.0 35.0 40.0 31.0 41.0 47 36.307776192234634 38.0 35.0 40.0 31.0 41.0 48 36.24975459132265 38.0 35.0 40.0 31.0 41.0 49 36.23422595907319 38.0 35.0 40.0 31.0 41.0 50 36.15148101894549 38.0 35.0 40.0 31.0 41.0 51 35.13391179885334 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 12.0 10 13.0 11 13.0 12 19.0 13 23.0 14 30.0 15 51.0 16 96.0 17 166.0 18 342.0 19 583.0 20 1118.0 21 1744.0 22 2588.0 23 3896.0 24 5574.0 25 7472.0 26 10013.0 27 12280.0 28 14974.0 29 18295.0 30 23213.0 31 30435.0 32 39864.0 33 56611.0 34 103323.0 35 131065.0 36 128494.0 37 201624.0 38 354837.0 39 467670.0 40 250.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08621746518042 20.77948105110995 24.661113348190842 11.473188135518784 2 31.919643271348697 23.960237299521058 25.660933350900077 18.459186078230164 3 32.518335291035825 23.90704222390055 26.557146665627506 17.01747581943612 4 28.43703589616074 25.638603790087284 26.47958082280411 19.44477949094787 5 25.61429487762349 29.635657030316864 24.046462805817562 20.70358528624208 6 26.820771563644506 33.51512441152947 24.174873244175913 15.489230780650107 7 79.29400237893327 5.845396553825067 9.173614500235356 5.6869865670063104 8 78.90425566790438 5.167654177576296 9.330416263837678 6.597673890681645 9 72.15942935291902 7.572999416709811 12.178579580142403 8.08899165022877 10 37.7210611056782 27.723294061759486 18.66806953214931 15.887575300413005 11 29.42961889439602 24.161883748966254 25.78080783526351 20.627689521374215 12 27.65568683193016 21.51635655793222 29.895508789249153 20.93244782088847 13 24.947191516498822 22.7415752298986 31.400249027179587 20.910984226422986 14 20.380765402912495 27.040108468470475 29.982971390327528 22.596154738289506 15 19.92693718218262 25.1499513512477 33.546855892684505 21.376255573885178 16 22.49724901047881 24.65505158375967 30.58487985644752 22.262819549314 17 21.020281550401407 24.5907845098414 28.98160501914095 25.407328920616244 18 22.880933957076522 24.532517345615208 29.665594724038176 22.92095397327009 19 22.728462025210757 26.39286047859486 27.69947998720844 23.179197508985947 20 25.13652887286439 26.09935974159564 27.344619349028353 21.419492036511613 21 23.325112838507792 27.309733276179553 27.8403850828637 21.524768802448953 22 21.656271977761985 24.492930312595295 27.99539305903231 25.855404650610414 23 22.15630568859479 25.984804764794262 27.813354561879787 24.045534984731155 24 23.5547176300233 23.446533691348563 28.52579744676008 24.472951231868056 25 21.588602893193567 25.576563486776383 26.246635875377542 26.588197744652504 26 20.83156274142678 25.12013736700458 27.748963778483333 26.29933611308531 27 21.938267733289788 24.946016276456046 27.17563220182459 25.940083788429575 28 20.013348252696403 26.14927651604419 27.700964500946686 26.13641073031272 29 22.148079008295337 23.848280221761613 27.341526612073675 26.662114157869375 30 24.074235583670596 22.716338496348406 27.654697156104664 25.554728763876337 31 24.731813314974847 24.59610401740345 25.443823216681484 25.22825945094022 32 23.567768979972055 25.08883886902321 26.46603463494261 24.87735751606213 33 24.153718923405894 23.949907558092427 27.038933228427698 24.857440290073985 34 22.563866564482638 22.950767957513214 30.05280539076422 24.432560087239924 35 20.66962703447969 24.460765848266615 28.406108526613927 26.463498590639773 36 22.53331032337039 26.130039692186074 27.551337887079224 23.785312097364308 37 22.581557019863414 25.545697971968668 29.45473191846803 22.418013089699887 38 22.058946329261435 27.139447179454823 28.124793173216155 22.676813318067584 39 24.303159973056076 22.788522976870656 29.590503070778524 23.317813979294744 40 23.178640816334102 22.66901962094179 30.593972503094285 23.558367059629823 41 19.392264817457384 25.306938679067304 28.242750160667683 27.058046342807625 42 21.257185201129964 24.11141028186586 28.562601016520784 26.068803500483394 43 22.65262811508198 22.21018116634533 29.134262515223995 26.00292820334869 44 22.574691143824023 22.058575200826873 27.934899124198747 27.431834531150358 45 23.407750769936865 20.511650030834588 28.49035468125944 27.590244517969108 46 23.568696801058458 23.01750922099523 28.521405760284434 24.89238821766188 47 20.374146945829473 23.49979062170817 30.993801536595427 25.132260895866924 48 20.43031104892648 22.42846654060671 31.18567493726383 25.955547473202984 49 22.907964478060432 21.05745624859667 31.71960504511994 24.314974228222958 50 20.86576841214555 20.643276915625805 31.585318406547696 26.90563626568095 51 20.39443530025218 20.604061011040454 29.521473181950046 29.48003050675732 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 796.0 1 1755.5 2 2715.0 3 4081.0 4 5447.0 5 3967.5 6 2488.0 7 2442.0 8 2396.0 9 2359.0 10 2322.0 11 2325.5 12 2329.0 13 2408.0 14 2487.0 15 2590.5 16 2694.0 17 2735.0 18 2776.0 19 2866.5 20 2957.0 21 3580.5 22 4204.0 23 4825.0 24 5446.0 25 6686.0 26 9352.5 27 10779.0 28 12951.0 29 15123.0 30 18135.0 31 21147.0 32 23637.0 33 26127.0 34 29761.0 35 33395.0 36 36445.5 37 39496.0 38 42636.0 39 45776.0 40 52623.5 41 59471.0 42 67956.0 43 76441.0 44 87619.5 45 98798.0 46 120314.0 47 141830.0 48 163335.0 49 184840.0 50 180991.5 51 177143.0 52 153364.5 53 129586.0 54 115194.5 55 100803.0 56 92226.5 57 83650.0 58 80161.0 59 76672.0 60 72432.5 61 68193.0 62 63523.0 63 58853.0 64 52120.0 65 45387.0 66 36941.0 67 28495.0 68 23090.0 69 17685.0 70 14593.0 71 11501.0 72 9423.0 73 7345.0 74 6099.5 75 3950.5 76 3047.0 77 2259.0 78 1471.0 79 1179.0 80 887.0 81 719.5 82 552.0 83 390.5 84 229.0 85 148.0 86 67.0 87 47.5 88 28.0 89 22.0 90 16.0 91 15.0 92 14.0 93 9.0 94 4.0 95 3.0 96 2.0 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1616691.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.373724582387904 #Duplication Level Percentage of deduplicated Percentage of total 1 68.66363560317865 18.795794498268563 2 12.66467928924733 6.933588855762572 3 4.974257663728447 4.084918778662044 4 2.5268615203016327 2.766784452582835 5 1.4313084604393 1.9590121794253526 6 0.971411604013925 1.5954692222647706 7 0.70218205666366 1.3454936758064007 8 0.5239661122696089 1.1474323238218251 9 0.4096204396439966 1.0091553388318268 >10 6.592825971334894 44.52469228689957 >50 0.44212449527451175 7.407629824330359 >100 0.08613130054587992 4.447088266776291 >500 0.007101249668774837 1.2981332853607204 >1k 0.003207015979446701 1.4586598630113552 >5k 6.872177098814358E-4 1.2261471481955137 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT 7032 0.4349625253063202 No Hit GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC 6738 0.4167772320127965 No Hit CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC 5784 0.35776781091748516 No Hit TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC 2805 0.17350254315759783 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCC 2632 0.16280167329440196 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2519 0.15581208777682315 No Hit ACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC 2032 0.12568882983823132 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2370947818723553E-4 0.0 0.0 0.4674981180695631 0.0 2 1.2370947818723553E-4 0.0 0.0 1.6193570694709132 0.0 3 1.2370947818723553E-4 0.0 0.0 2.3439853379526454 0.0 4 1.2370947818723553E-4 0.0 0.0 3.1879313981459663 0.0 5 1.2370947818723553E-4 0.0 0.0 5.096521227618636 0.0 6 1.2370947818723553E-4 0.0 0.0 6.230380449943743 0.0 7 1.2370947818723553E-4 0.0 0.0 7.392569142773727 0.0 8 1.2370947818723553E-4 0.0 0.0 9.239737216326434 0.0 9 1.2370947818723553E-4 0.0 0.0 9.910675571274906 0.0 10 1.2370947818723553E-4 0.0 0.0 11.312613232831753 0.0 11 1.2370947818723553E-4 0.0 0.0 13.296171006085888 0.0 12 1.2370947818723553E-4 0.0 0.0 14.860786631458948 0.0 13 1.2370947818723553E-4 0.0 0.0 15.482736033045276 0.0 14 1.2370947818723553E-4 0.0 0.0 15.731763212636181 0.0 15 1.2370947818723553E-4 0.0 0.0 16.242559648071275 0.0 16 1.855642172808533E-4 0.0 0.0 17.18367950338067 0.0 17 1.855642172808533E-4 0.0 0.0 18.3299096735245 0.0 18 1.855642172808533E-4 0.0 0.0 19.571086868177037 0.0 19 1.855642172808533E-4 0.0 0.0 20.363940913879027 0.0 20 1.855642172808533E-4 0.0 0.0 21.121661467775844 0.0 21 1.855642172808533E-4 0.0 0.0 22.038781684316916 0.0 22 1.855642172808533E-4 0.0 0.0 22.994994095965154 0.0 23 1.855642172808533E-4 0.0 0.0 23.90710407863964 0.0 24 1.855642172808533E-4 0.0 0.0 24.648742400372118 0.0 25 1.855642172808533E-4 0.0 0.0 25.30087691463613 0.0 26 1.855642172808533E-4 0.0 0.0 25.882620735811606 0.0 27 1.855642172808533E-4 0.0 0.0 26.48539516827891 0.0 28 1.855642172808533E-4 0.0 0.0 27.09973643695672 0.0 29 1.855642172808533E-4 0.0 0.0 27.73795363492467 0.0 30 1.855642172808533E-4 0.0 0.0 28.46629318775202 0.0 31 1.855642172808533E-4 0.0 0.0 29.10166506772166 0.0 32 3.092736954680888E-4 0.0 0.0 29.7564593357667 0.0 33 4.3298317365532434E-4 0.0 0.0 30.377790189962088 0.0 34 4.3298317365532434E-4 0.0 0.0 30.946111532754248 0.0 35 4.3298317365532434E-4 0.0 0.0 31.57034955968704 0.0 36 4.3298317365532434E-4 0.0 0.0 32.16347465285574 0.0 37 4.3298317365532434E-4 0.0 0.0 32.7734242350579 0.0 38 4.948379127489421E-4 0.0 0.0 33.404899266464646 0.0 39 4.948379127489421E-4 0.0 0.0 34.03321970617762 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGCG 20 7.0341385E-4 45.0 1 CGTCTAA 20 7.0341385E-4 45.0 18 TCGAACG 25 3.8915125E-5 45.0 1 CGGTCTA 120 0.0 41.249996 31 CGACGGT 130 0.0 39.80769 28 TACGGGT 85 0.0 39.705883 4 CTAACGG 85 0.0 39.705883 2 CGTATCC 35 6.249864E-6 38.57143 43 AATGCGA 70 0.0 38.57143 25 ACGTATC 35 6.249864E-6 38.57143 42 GTACGAG 135 0.0 38.333336 1 ACGTAAG 100 0.0 38.25 1 TAGTGCG 100 0.0 38.25 1 CGTTTTT 2105 0.0 37.73159 1 CGAGGGT 245 0.0 37.65306 4 CGTCGTG 30 1.1401275E-4 37.499996 16 GCCCGAT 30 1.1401275E-4 37.499996 8 CACGACG 135 0.0 36.666668 26 TATGGGA 1005 0.0 36.49254 4 TAGGGAT 1030 0.0 36.480583 5 >>END_MODULE