FastQCFastQC Report
Sat 18 Jun 2016
SRR3550007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003808
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC37610.3746732442857598TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT35210.3507642895852593TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC30690.30573575823265003TruSeq Adapter, Index 16 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT16350.1628797538971596No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15770.15710175651120534No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCC14800.14743855398641972No Hit
TCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC13740.13687876566036533TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC11470.11426487933947527TruSeq Adapter, Index 13 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11160.1111766393573273No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10520.1048009181038605No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG253.8904516E-545.01
AACTACG253.8904516E-545.01
CGTATGG1400.043.392862
GTTAGCG603.6379788E-1241.2500041
TATGCGG1300.039.807692
GTACACG403.4576442E-739.3750041
TCGATCA1800.038.7517
CTCGATC1800.038.7516
TTATGCG700.038.571431
TATTGCG356.2474955E-638.571431
ATAACGG356.2474955E-638.571432
ATTCGGC1350.038.33333217
CCCACGA1650.038.18181639
GTTACAC1300.038.0769233
CGTTAGT301.1398188E-437.50000445
TCTACGG301.1398188E-437.5000042
CATCGTT301.1398188E-437.50000436
CACGTTA301.1398188E-437.50000443
CCTATAG301.1398188E-437.5000041
ACGTATG900.037.51
Produced by FastQC (version 0.11.5)