FastQCFastQC Report
Sat 18 Jun 2016
SRR3550006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853816
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC36220.4242131794203904No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT32430.3798242244230607Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC26810.3140020800734584No Hit
TCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC13640.15975338948907025No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.13586065381768436No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC10600.12414852848857366No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC10340.12110337590300486No Hit
ACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC9780.11454458571870285No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA8600.1007242778303522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGCG453.8380676E-1045.0000041
AACGTGT253.8899605E-545.027
CTCGTAG207.032266E-445.01
TCGATAG207.032266E-445.01
GCCGATT406.8121153E-945.09
TACGCGG406.8121153E-945.02
CGAATAT700.045.014
ATGCGAA253.8899605E-545.026
CCGGTTA207.032266E-445.020
CGGTCTA800.045.031
ACGTAAG253.8899605E-545.01
ATACGCC207.032266E-445.012
CGAAGTT207.032266E-445.015
CTACGAA750.042.00000411
TGATTCG700.041.78571315
ACGTAGG556.002665E-1140.9090922
CACAACG1100.040.90909212
GCATATC501.0804797E-940.528
ACGGGAC2350.040.2127655
ATAACGC850.039.70588311