##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	853816
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33166045143216	33.0	31.0	34.0	30.0	34.0
2	32.485474622166834	34.0	31.0	34.0	31.0	34.0
3	32.48828787467089	34.0	31.0	34.0	31.0	34.0
4	36.018930308169445	37.0	35.0	37.0	35.0	37.0
5	35.9047722225866	37.0	35.0	37.0	35.0	37.0
6	35.86119491787458	37.0	35.0	37.0	35.0	37.0
7	36.059841933156555	37.0	35.0	37.0	35.0	37.0
8	36.02149994846665	37.0	35.0	37.0	35.0	37.0
9	37.55281582917163	39.0	38.0	39.0	35.0	39.0
10	37.36981738454187	39.0	37.0	39.0	34.0	39.0
11	37.292790249889904	39.0	37.0	39.0	34.0	39.0
12	37.17265078190149	39.0	37.0	39.0	34.0	39.0
13	37.09284201748386	39.0	37.0	39.0	33.0	39.0
14	38.254491600063716	40.0	37.0	41.0	33.0	41.0
15	38.34988803208186	40.0	38.0	41.0	34.0	41.0
16	38.370631377252245	40.0	37.0	41.0	34.0	41.0
17	38.40751520233868	40.0	37.0	41.0	34.0	41.0
18	38.35688134211586	40.0	37.0	41.0	34.0	41.0
19	38.318880180273034	40.0	37.0	41.0	34.0	41.0
20	38.28232312348328	40.0	37.0	41.0	34.0	41.0
21	38.254368622747755	40.0	37.0	41.0	34.0	41.0
22	38.15494790446654	40.0	37.0	41.0	34.0	41.0
23	37.91045494579628	40.0	36.0	41.0	33.0	41.0
24	37.67434786886167	40.0	36.0	41.0	33.0	41.0
25	37.667046529931504	39.0	36.0	41.0	33.0	41.0
26	37.76141346613322	40.0	36.0	41.0	33.0	41.0
27	37.810942872937495	40.0	36.0	41.0	33.0	41.0
28	37.77690977915616	40.0	36.0	41.0	33.0	41.0
29	37.766196698117625	40.0	36.0	41.0	33.0	41.0
30	37.686312976097895	40.0	36.0	41.0	33.0	41.0
31	37.57510166124786	40.0	36.0	41.0	33.0	41.0
32	37.44657865394886	39.0	36.0	41.0	33.0	41.0
33	37.37026713015451	39.0	35.0	41.0	33.0	41.0
34	37.35455297160044	39.0	35.0	41.0	33.0	41.0
35	37.284275534775645	39.0	35.0	41.0	33.0	41.0
36	37.19205191750916	39.0	35.0	41.0	32.0	41.0
37	37.16041043972003	39.0	35.0	41.0	32.0	41.0
38	37.052971600438504	39.0	35.0	41.0	32.0	41.0
39	36.99022154656272	39.0	35.0	41.0	32.0	41.0
40	36.96279994752968	39.0	35.0	41.0	32.0	41.0
41	36.9014307532302	39.0	35.0	41.0	32.0	41.0
42	36.84048085301751	39.0	35.0	41.0	32.0	41.0
43	36.75347030273502	39.0	35.0	41.0	31.0	41.0
44	36.67462661744451	39.0	35.0	41.0	31.0	41.0
45	36.60469937316705	39.0	35.0	41.0	31.0	41.0
46	36.51098128870857	39.0	35.0	40.0	31.0	41.0
47	36.41199743270213	38.0	35.0	40.0	31.0	41.0
48	36.3258219569556	38.0	35.0	40.0	31.0	41.0
49	36.30642316377299	38.0	35.0	40.0	31.0	41.0
50	36.230179570305545	38.0	35.0	40.0	31.0	41.0
51	35.24525073317905	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	4.0
11	6.0
12	11.0
13	12.0
14	20.0
15	28.0
16	54.0
17	110.0
18	177.0
19	306.0
20	549.0
21	928.0
22	1555.0
23	2138.0
24	2995.0
25	4050.0
26	5159.0
27	6399.0
28	7652.0
29	9356.0
30	11624.0
31	14964.0
32	19962.0
33	27925.0
34	51869.0
35	70906.0
36	66456.0
37	102847.0
38	182949.0
39	262599.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.365081001058776	21.157954407038517	24.540064838325822	10.936899753576883
2	31.888954997329634	24.907239967393444	25.366238159041295	17.83756687623563
3	32.13514387174754	24.026253900137736	26.211736486549796	17.626865741564927
4	28.931409109222596	25.70870070366449	25.69593448705576	19.663955700057155
5	25.086786848688707	30.330656722291454	24.24456791627236	20.337988512747476
6	26.918680371414915	34.17106261770686	23.792596999821974	15.117660011056246
7	79.25220422198694	6.992255942732392	8.375457944100368	5.380081891180301
8	79.11329841558369	6.0780074395420085	8.680441687670411	6.128252457203894
9	72.3014092029196	7.989309171999588	11.565723762496837	8.143557862583977
10	38.85228199049912	26.77391850234711	18.585268957246058	15.788530549907708
11	29.52111461954332	25.286244343043467	24.517694678947223	20.67494635846599
12	26.34701153410102	22.513281550123214	30.017825854750907	21.121881061024858
13	24.340373101464486	24.31987688213854	31.170064744628817	20.169685271768156
14	20.336465936454694	28.29614343137163	29.454823990180557	21.912566641993124
15	18.95361529884659	26.05163173330085	33.590961050156004	21.40379191769655
16	20.692163182699787	25.020847583085814	31.425506198056723	22.861483036157672
17	21.77471492686949	25.02646940324379	27.759493848791777	25.439321821094946
18	22.49922699972828	24.826894787635744	29.63109147638367	23.042786736252307
19	22.89638516963842	26.320425009603944	27.2719180713409	23.511271749416736
20	24.158366673850104	26.466123848698082	27.29663065578532	22.078878821666496
21	23.45235975901131	26.738079398840032	28.37906527870173	21.430495563446925
22	21.652557459686864	25.018270915513412	27.02444086313679	26.304730761662938
23	21.42791889587452	26.579497221883873	27.213474565948637	24.77910931629297
24	22.754434210649602	24.90993375621914	28.73733919251923	23.59829284061203
25	21.558509093294106	26.31164091560711	26.505827953563767	25.62402203753502
26	20.252021512831806	26.991061305948822	27.09658755516411	25.660329626055262
27	22.268146766984927	26.646841942526255	27.34383052086164	23.741180769627178
28	20.35110609311608	26.575749341778554	28.105118667253837	24.96802589785153
29	21.065311495685254	25.79138830848801	27.791116587180376	25.35218360864636
30	22.865113795009698	27.275431708939628	25.864940455554823	23.994514040495847
31	24.128149390500997	27.89512026010288	23.5380925164204	24.438637832975722
32	23.6197260299643	27.297918989571524	25.241855388046137	23.840499592418038
33	22.511876095083718	27.590370759039416	25.930294114891268	23.967459030985598
34	21.67223383023977	25.532433217461374	27.08827194618044	25.707061006118415
35	22.336194215147056	24.507973614924058	27.00769252391616	26.148139646012723
36	22.47381168776411	28.05674758964461	26.38484169891405	23.08459902367723
37	22.697864645309995	27.030414047054634	27.764412941430006	22.507308366205365
38	22.841338180591602	27.602902733141566	26.765720014616733	22.7900390716501
39	22.385502262782612	25.350426789846992	28.124443674046866	24.139627273323526
40	22.63368219850647	24.241171399926916	28.466789097416772	24.658357304149842
41	20.98590328595388	25.134923683791357	26.615804810404114	27.263368219850648
42	22.04538214322524	24.98957620845709	28.201392337459126	24.763649310858547
43	23.350464268648047	23.580841773871654	29.257357557131748	23.811336400348555
44	22.243668425047083	24.64465411751478	27.0058185838635	26.105858873574633
45	21.961640447122097	23.661421196135933	27.616957283536497	26.75998107320547
46	22.86862743260843	25.139022927656544	27.68957246057699	24.30277717915804
47	21.573032128702202	24.137050605751124	29.395794878521837	24.89412238702484
48	20.863394455011385	23.939584172702315	29.527439167221043	25.66958220506526
49	22.24331706128721	22.721757380981384	31.05610576517657	23.978819792554837
50	20.90017052854479	22.51831776401473	30.157083024914034	26.424428682526447
51	20.91036007758112	22.909268507500443	27.118606350782837	29.061765064135596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	560.0
1	1023.0
2	1486.0
3	2021.5
4	2557.0
5	1913.5
6	1270.0
7	1265.5
8	1261.0
9	1326.5
10	1392.0
11	1463.0
12	1534.0
13	1612.0
14	1690.0
15	1694.0
16	1698.0
17	1628.0
18	1558.0
19	1681.0
20	1804.0
21	2137.0
22	2470.0
23	2605.0
24	2740.0
25	3616.5
26	5444.5
27	6396.0
28	7562.5
29	8729.0
30	10736.5
31	12744.0
32	14249.0
33	15754.0
34	17534.0
35	19314.0
36	20337.0
37	21360.0
38	24794.0
39	28228.0
40	31734.0
41	35240.0
42	39704.0
43	44168.0
44	50488.5
45	56809.0
46	65802.0
47	74795.0
48	85526.0
49	96257.0
50	92728.0
51	89199.0
52	76535.5
53	63872.0
54	56978.0
55	50084.0
56	44867.0
57	39650.0
58	38877.5
59	38105.0
60	36139.5
61	34174.0
62	30409.5
63	26645.0
64	24234.5
65	21824.0
66	18678.5
67	15533.0
68	12971.0
69	10409.0
70	8709.0
71	7009.0
72	6013.5
73	5018.0
74	3893.0
75	2265.5
76	1763.0
77	1244.5
78	726.0
79	563.5
80	401.0
81	313.0
82	225.0
83	150.5
84	76.0
85	45.0
86	14.0
87	10.0
88	6.0
89	3.5
90	1.0
91	3.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	853816.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.9625619304241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.76342991913535	19.787267915230476
2	11.789795622863403	6.59345780502722
3	4.477707117408294	3.7562448773049066
4	2.2104410117227786	2.4723837473539003
5	1.2971573866285173	1.81359218785535
6	0.8715046794187843	1.4621702142541297
7	0.5829414907829716	1.1410376276482816
8	0.4919762277916639	1.100553259033667
9	0.41795904063606715	1.0518485002350004
>10	6.5271737234086205	45.793821584246594
>50	0.4800166662889096	8.10163820550535
>100	0.0826883830818387	4.397408800634337
>500	0.004240429901632753	0.8491032848336971
>1k	0.0029683009311429276	1.6794719908371016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	3622	0.4242131794203904	No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT	3243	0.3798242244230607	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	2681	0.3140020800734584	No Hit
TCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	1364	0.15975338948907025	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1160	0.13586065381768436	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1060	0.12414852848857366	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC	1034	0.12110337590300486	No Hit
ACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	978	0.11454458571870285	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	860	0.1007242778303522	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3424250658221445E-4	0.0	0.0	0.40336559633457325	0.0
2	2.3424250658221445E-4	0.0	0.0	1.4853317342378218	0.0
3	2.3424250658221445E-4	0.0	0.0	2.139219691362073	0.0
4	2.3424250658221445E-4	0.0	0.0	2.867596765579469	0.0
5	2.3424250658221445E-4	0.0	0.0	4.5685487271262195	0.0
6	2.3424250658221445E-4	0.0	0.0	5.526014972781021	0.0
7	2.3424250658221445E-4	0.0	0.0	6.507491075360499	0.0
8	2.3424250658221445E-4	0.0	0.0	8.152341956580809	0.0
9	2.3424250658221445E-4	0.0	0.0	8.748840499592418	0.0
10	2.3424250658221445E-4	0.0	0.0	9.983298509280688	0.0
11	2.3424250658221445E-4	0.0	0.0	11.7374235198216	0.0
12	2.3424250658221445E-4	0.0	0.0	13.09450748170566	0.0
13	2.3424250658221445E-4	0.0	0.0	13.661140105128037	0.0
14	2.3424250658221445E-4	0.0	0.0	13.884607456407469	0.0
15	2.3424250658221445E-4	0.0	0.0	14.33107367395317	0.0
16	2.3424250658221445E-4	0.0	0.0	15.215807621314195	0.0
17	2.3424250658221445E-4	0.0	0.0	16.26486268704264	0.0
18	2.3424250658221445E-4	0.0	0.0	17.47484235479307	0.0
19	2.3424250658221445E-4	0.0	0.0	18.166911840490222	0.0
20	2.3424250658221445E-4	0.0	0.0	18.838602228114723	0.0
21	2.3424250658221445E-4	0.0	0.0	19.641585540678555	0.0
22	2.3424250658221445E-4	0.0	0.0	20.470921135232885	0.0
23	2.3424250658221445E-4	0.0	0.0	21.25094868215166	0.0
24	2.3424250658221445E-4	0.0	0.0	21.887619815042118	0.0
25	2.3424250658221445E-4	0.0	0.0	22.45085592211905	0.0
26	2.3424250658221445E-4	0.0	0.0	23.00401960141295	0.0
27	2.3424250658221445E-4	0.0	0.0	23.525092057305088	0.0
28	3.5136375987332164E-4	0.0	0.0	24.083291950490505	0.0
29	3.5136375987332164E-4	0.0	0.0	24.634347447225164	0.0
30	3.5136375987332164E-4	0.0	0.0	25.211872347203613	0.0
31	3.5136375987332164E-4	0.0	0.0	25.765270269004095	0.0
32	3.5136375987332164E-4	0.0	0.0	26.310118339314325	0.0
33	3.5136375987332164E-4	0.0	0.0	26.850750044506075	0.0
34	3.5136375987332164E-4	0.0	0.0	27.354137191151256	0.0
35	3.5136375987332164E-4	0.0	0.0	27.88879571242516	0.0
36	3.5136375987332164E-4	0.0	0.0	28.424859688738557	0.0
37	3.5136375987332164E-4	0.0	0.0	28.941950022018794	0.0
38	3.5136375987332164E-4	0.0	0.0	29.46782444929587	0.0
39	3.5136375987332164E-4	0.0	0.0	29.98432917630965	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATAGCG	45	3.8380676E-10	45.000004	1
AACGTGT	25	3.8899605E-5	45.0	27
CTCGTAG	20	7.032266E-4	45.0	1
TCGATAG	20	7.032266E-4	45.0	1
GCCGATT	40	6.8121153E-9	45.0	9
TACGCGG	40	6.8121153E-9	45.0	2
CGAATAT	70	0.0	45.0	14
ATGCGAA	25	3.8899605E-5	45.0	26
CCGGTTA	20	7.032266E-4	45.0	20
CGGTCTA	80	0.0	45.0	31
ACGTAAG	25	3.8899605E-5	45.0	1
ATACGCC	20	7.032266E-4	45.0	12
CGAAGTT	20	7.032266E-4	45.0	15
CTACGAA	75	0.0	42.000004	11
TGATTCG	70	0.0	41.785713	15
ACGTAGG	55	6.002665E-11	40.909092	2
CACAACG	110	0.0	40.909092	12
GCATATC	50	1.0804797E-9	40.5	28
ACGGGAC	235	0.0	40.212765	5
ATAACGC	85	0.0	39.705883	11
>>END_MODULE