##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550001_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651596
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20810287355969	33.0	31.0	34.0	30.0	34.0
2	32.37564687321592	34.0	31.0	34.0	30.0	34.0
3	32.36954953682957	34.0	31.0	34.0	30.0	34.0
4	35.929270590979684	37.0	35.0	37.0	35.0	37.0
5	35.804694933670554	37.0	35.0	37.0	35.0	37.0
6	35.762547652226225	37.0	35.0	37.0	35.0	37.0
7	36.01332267233071	37.0	35.0	37.0	35.0	37.0
8	35.98916199608346	37.0	35.0	37.0	35.0	37.0
9	37.529992510696815	39.0	38.0	39.0	35.0	39.0
10	37.284572035433	39.0	37.0	39.0	34.0	39.0
11	37.20590519278817	39.0	37.0	39.0	34.0	39.0
12	37.13162143414017	39.0	37.0	39.0	34.0	39.0
13	37.06618978630931	39.0	37.0	39.0	33.0	39.0
14	38.186617167692866	40.0	37.0	41.0	33.0	41.0
15	38.28678813252383	40.0	37.0	41.0	34.0	41.0
16	38.30606234537965	40.0	37.0	41.0	34.0	41.0
17	38.34102265821153	40.0	37.0	41.0	34.0	41.0
18	38.28979613134519	40.0	37.0	41.0	34.0	41.0
19	38.23526540985519	40.0	37.0	41.0	34.0	41.0
20	38.19951166059951	40.0	37.0	41.0	34.0	41.0
21	38.18667395134408	40.0	37.0	41.0	34.0	41.0
22	38.082934824645946	40.0	37.0	41.0	34.0	41.0
23	37.82975187079111	40.0	36.0	41.0	33.0	41.0
24	37.62725062768955	39.0	36.0	41.0	33.0	41.0
25	37.6476773951958	39.0	36.0	41.0	33.0	41.0
26	37.72819968201156	40.0	36.0	41.0	33.0	41.0
27	37.79049748617241	40.0	36.0	41.0	33.0	41.0
28	37.78563711256668	40.0	36.0	41.0	33.0	41.0
29	37.7753822920951	40.0	36.0	41.0	33.0	41.0
30	37.67181965512373	40.0	36.0	41.0	33.0	41.0
31	37.545898071811365	40.0	36.0	41.0	33.0	41.0
32	37.42847408516934	40.0	36.0	41.0	33.0	41.0
33	37.32517234605492	39.0	36.0	41.0	32.0	41.0
34	37.31126649027925	40.0	36.0	41.0	32.0	41.0
35	37.249811232727026	40.0	35.0	41.0	32.0	41.0
36	37.095769464514824	39.0	35.0	41.0	32.0	41.0
37	37.0962221990313	39.0	35.0	41.0	32.0	41.0
38	37.00030386926869	39.0	35.0	41.0	32.0	41.0
39	36.92383163800883	39.0	35.0	41.0	31.0	41.0
40	36.87830956605013	39.0	35.0	41.0	31.0	41.0
41	36.799869858010176	39.0	35.0	41.0	31.0	41.0
42	36.74265956205993	39.0	35.0	41.0	31.0	41.0
43	36.65129006316798	39.0	35.0	41.0	31.0	41.0
44	36.55658874517339	39.0	35.0	41.0	31.0	41.0
45	36.4757978870343	39.0	35.0	41.0	31.0	41.0
46	36.32373126906857	39.0	35.0	40.0	31.0	41.0
47	36.21241536166582	38.0	35.0	40.0	30.0	41.0
48	36.14804418688881	38.0	35.0	40.0	30.0	41.0
49	36.102078588573285	38.0	35.0	40.0	30.0	41.0
50	35.98883357172235	38.0	35.0	40.0	30.0	41.0
51	34.92917697468984	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	3.0
12	11.0
13	9.0
14	15.0
15	13.0
16	46.0
17	88.0
18	136.0
19	283.0
20	508.0
21	824.0
22	1202.0
23	1756.0
24	2568.0
25	3301.0
26	4280.0
27	5226.0
28	6205.0
29	7507.0
30	9454.0
31	11979.0
32	15943.0
33	22085.0
34	38608.0
35	51614.0
36	52860.0
37	81342.0
38	144667.0
39	188928.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.69961141566246	22.100810931927146	25.55755406724412	10.64202358516627
2	33.30038858433754	23.516872417878563	25.083487314225376	18.099251683558524
3	31.888010362249002	23.47359406748967	27.45811822049245	17.180277349768875
4	28.71564589101222	26.042824081179134	26.226987274323356	19.01454275348529
5	24.523170799084095	30.19340204666695	25.26135826493717	20.022068889311782
6	26.276404397817053	34.29747880588586	24.361107189117185	15.065009607179908
7	77.99219148061069	6.414710955868361	9.559450948133506	6.033646615387449
8	77.3336545957925	5.5925757678070465	10.086771557836451	6.986998078564017
9	71.30369124426792	7.620365993652508	12.837248847445348	8.23869391463422
10	37.909532900754456	24.25459947574878	20.753196766094327	17.08267085740244
11	28.19952854222555	25.34453864050731	25.22989705277503	21.22603576449211
12	26.547124291739056	22.528683417332214	30.763694068103547	20.160498222825186
13	24.12369013928876	25.256754185108566	31.100405772902228	19.51914990270045
14	19.15113045506725	29.713656928526266	29.150271026832574	21.984941589573907
15	18.06241904492968	26.548352046360012	34.249596375668354	21.139632533041947
16	19.820870600801722	25.559702637830807	32.15566086961859	22.463765891748874
17	20.168325158533815	25.613416902497864	28.083045322561834	26.135212616406484
18	21.129963965401874	26.236041964652944	30.21795713908618	22.416036930858997
19	21.9955309731797	27.829513993333293	27.601765511144944	22.573189522342066
20	24.158834615313786	26.57781815726309	27.783473195047236	21.47987403237589
21	22.61186379290235	27.453207202008606	28.629089190234442	21.3058398148546
22	20.363538143266688	26.554797758120063	26.29742969570102	26.784234402912233
23	20.549542968342347	27.286079104230225	27.415760686069284	24.748617241358144
24	21.950717929514607	25.292819477099304	28.540690857525213	24.215771735860873
25	21.31781042240898	26.390585577566466	25.95197023922799	26.339633760796566
26	20.792791852620336	28.314170129957827	27.053266134230412	23.839771883191425
27	22.698573963007753	28.12202653177736	26.661152002160847	22.518247503054038
28	21.066427663767122	27.100227748482187	27.603300204421146	24.230044383329545
29	23.6821588837255	25.976985739630077	26.228368498271937	24.11248687837249
30	24.337012504680814	28.214722005659947	25.265501936782915	22.182763552876324
31	24.55309731797003	28.680654884314823	24.209786432083682	22.556461365631463
32	25.43339738119939	28.45305987145409	22.901306944794015	23.212235802552502
33	23.105421150528855	30.779501408848425	23.59130504177435	22.523772398848365
34	23.230498652539303	26.380303132615918	26.794056439879927	23.595141774964855
35	23.975131830152428	25.744633177613125	24.723294802300813	25.55694018993364
36	22.792497191511305	31.116213113647106	24.339468013922737	21.75182168091885
37	23.40468633938821	28.56401819532348	27.302653791613206	20.7286416736751
38	22.715455589045973	28.62862878225158	24.556473643177675	24.099441985524773
39	23.45471734019239	27.579819397295257	26.53469327620182	22.430769986310537
40	23.105114211873616	24.717616437178865	29.896285428394282	22.280983922553236
41	19.752883688665985	26.79958133567425	26.321984788120247	27.12555018753952
42	23.122763184549935	27.206121584540117	25.74893031878649	23.922184912123463
43	24.14747788506989	26.374010890183484	26.584724277006	22.893786947740622
44	24.110338307785806	25.316914161535674	25.713325434778607	24.859422095899912
45	24.239099073659137	23.748918041240277	26.234046863393885	25.777936021706704
46	23.507050380910872	25.632907507105628	27.122480800987113	23.737561310996384
47	21.05860072805849	25.72023155452151	29.737905082290254	23.48326263512974
48	20.84696652527026	24.31568026814161	28.752325060313446	26.085028146274684
49	22.697346208386794	23.829029030258013	29.477160694663564	23.99646406669163
50	21.076249700734813	23.013799961939608	29.656259399996316	26.253690937329267
51	20.84758040258074	22.766253936488255	26.446294943492592	29.93987071743841
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	446.0
1	798.0
2	1150.0
3	1639.5
4	2129.0
5	1612.0
6	1095.0
7	1116.0
8	1137.0
9	1179.5
10	1222.0
11	1311.0
12	1400.0
13	1434.0
14	1468.0
15	1473.0
16	1478.0
17	1522.0
18	1566.0
19	1552.0
20	1538.0
21	1775.0
22	2012.0
23	2128.0
24	2244.0
25	2668.5
26	3830.0
27	4567.0
28	5842.0
29	7117.0
30	7737.0
31	8357.0
32	9689.0
33	11021.0
34	12780.5
35	14540.0
36	15913.0
37	17286.0
38	18576.5
39	19867.0
40	22433.0
41	24999.0
42	29311.5
43	33624.0
44	38928.0
45	44232.0
46	54370.5
47	64509.0
48	73244.0
49	81979.0
50	78571.5
51	75164.0
52	63087.5
53	51011.0
54	43840.0
55	36669.0
56	32094.5
57	27520.0
58	26045.5
59	24571.0
60	23145.5
61	21720.0
62	20468.5
63	19217.0
64	16886.5
65	14556.0
66	12004.0
67	9452.0
68	7989.5
69	6527.0
70	5325.0
71	4123.0
72	3540.0
73	2957.0
74	2230.0
75	1354.0
76	1205.0
77	904.0
78	603.0
79	533.5
80	464.0
81	305.0
82	146.0
83	96.5
84	47.0
85	36.5
86	26.0
87	30.0
88	34.0
89	19.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	651596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.74944899933956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63642634632733	25.967122191623133
2	11.577609944583442	8.277863524962646
3	4.037113496658027	4.329737491599646
4	2.0161572253532887	2.8830603960966945
5	1.2794679852087916	2.2870137741754717
6	0.872592551179849	1.8716821743364562
7	0.69899901334713	1.749218070476933
8	0.579992227366606	1.6587522041804668
9	0.4721271573809258	1.5190457160593311
>10	5.678646197692308	40.62365717430991
>50	0.09190800357618736	2.1837243514359215
>100	0.0528904548660005	3.5578123630167617
>500	0.003468226548590197	0.921379791694313
>1k	0.002601169911442648	2.1699307760323086
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	3731	0.5725940613508984	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	3432	0.526706732392464	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	3012	0.46224961479198773	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC	1396	0.21424318135777384	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	1348	0.2068766536320051	No Hit
ACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	1082	0.16605381248503676	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTG	968	0.14855830913633603	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	925	0.14195912804866817	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTG	804	0.12338933940662618	No Hit
GGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTG	735	0.11279995580083366	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTG	681	0.10451261210934382	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.069386552403637E-4	0.0	0.0	0.6074315987206796	0.0
2	3.069386552403637E-4	0.0	0.0	2.053266134230413	0.0
3	3.069386552403637E-4	0.0	0.0	3.014137594460371	0.0
4	3.069386552403637E-4	0.0	0.0	3.9826825210713386	0.0
5	3.069386552403637E-4	0.0	0.0	6.198779611906764	0.0
6	3.069386552403637E-4	0.0	0.0	7.511095832386939	0.0
7	3.069386552403637E-4	0.0	0.0	8.792104310032597	0.0
8	3.069386552403637E-4	0.0	0.0	10.905683890017741	0.0
9	3.069386552403637E-4	0.0	0.0	11.662287675185237	0.0
10	3.069386552403637E-4	0.0	0.0	13.26926500469616	0.0
11	3.069386552403637E-4	0.0	0.0	15.755161173487867	0.0
12	3.069386552403637E-4	0.0	0.0	17.42183807144304	0.0
13	3.069386552403637E-4	0.0	0.0	18.141609217981696	0.0
14	3.069386552403637E-4	0.0	0.0	18.421997679543768	0.0
15	3.069386552403637E-4	0.0	0.0	18.977096237545965	0.0
16	3.069386552403637E-4	0.0	0.0	20.152978225771797	0.0
17	3.069386552403637E-4	0.0	0.0	21.585153991123335	0.0
18	3.069386552403637E-4	0.0	0.0	23.202874173567672	0.0
19	3.069386552403637E-4	0.0	0.0	24.075500770416024	0.0
20	3.069386552403637E-4	0.0	0.0	24.923418805517528	0.0
21	3.069386552403637E-4	0.0	0.0	25.96179227619568	0.0
22	3.069386552403637E-4	0.0	0.0	27.045285729194163	0.0
23	3.069386552403637E-4	0.0	0.0	28.155329375870938	0.0
24	3.069386552403637E-4	0.0	0.0	29.00953965340487	0.0
25	3.069386552403637E-4	0.0	0.0	29.72547406675302	0.0
26	3.069386552403637E-4	0.0	0.0	30.40089257760944	0.0
27	3.069386552403637E-4	0.0	0.0	31.048840078821847	0.0
28	3.069386552403637E-4	0.0	0.0	31.706916555657187	0.0
29	4.6040798286054547E-4	0.0	0.0	32.387706492980314	0.0
30	4.6040798286054547E-4	0.0	0.0	33.13494864916298	0.0
31	4.6040798286054547E-4	0.0	0.0	33.84980877721779	0.0
32	4.6040798286054547E-4	0.0	0.0	34.52722238933327	0.0
33	4.6040798286054547E-4	0.0	0.0	35.173635197269476	0.0
34	4.6040798286054547E-4	0.0	0.0	35.81145372285895	0.0
35	4.6040798286054547E-4	0.0	0.0	36.43377184635879	0.0
36	4.6040798286054547E-4	0.0	0.0	37.0642238442225	0.0
37	4.6040798286054547E-4	0.0	0.0	37.68976482360235	0.0
38	4.6040798286054547E-4	0.0	0.0	38.286760508044864	0.0
39	4.6040798286054547E-4	0.0	0.0	38.92104923909908	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	25	3.888939E-5	45.0	1
CCGATAT	20	7.0310355E-4	45.0	12
GCTCTAG	20	7.0310355E-4	45.0	1
TCGTGTT	20	7.0310355E-4	45.0	23
ATATACG	25	3.888939E-5	45.0	1
CTCGACA	20	7.0310355E-4	45.0	38
CGGTCTA	40	6.8066583E-9	45.0	31
ATGCACG	25	3.888939E-5	45.0	1
TGAGACG	20	7.0310355E-4	45.0	10
CGCACGG	30	2.164048E-6	44.999996	2
GCATCGC	60	3.6379788E-12	41.249996	22
CGAATAT	115	0.0	41.086956	14
GCTACGA	115	0.0	41.086956	10
CGTGCGG	55	6.002665E-11	40.909092	2
TCGTTTA	45	1.9261279E-8	40.0	38
CCGGGAC	45	1.9261279E-8	40.0	5
CCTATGC	90	0.0	40.0	35
TATACGG	45	1.9261279E-8	40.0	2
TGTTGCG	40	3.4551522E-7	39.375	1
TGATTCG	40	3.4551522E-7	39.375	15
>>END_MODULE