FastQCFastQC Report
Sat 18 Jun 2016
SRR3549998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136476
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT52900.4654739739334574No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC46160.40616783812416635No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC40170.3534610497713986No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC21160.186189589573383No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.1652476603113484No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC17450.15354481748844673No Hit
ACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC13920.12248388879307615No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG207.0332544E-445.06
TACGTAG351.2119199E-745.01
CGCATCG451.9283107E-840.021
CGTAAGG1300.039.8076932
TCGTTAG403.4581717E-739.3751
TTTAGCG356.2482286E-638.5714261
CGGTTGA356.2482286E-638.57142630
TGCGTAG700.038.5714261
CGAGGGT2350.038.297874
TATGGGA5650.038.230094
TTACGAG659.094947E-1238.0769271
AACGTTG301.1399138E-437.4999962
TGTTACG301.1399138E-437.4999961
ATAACGC601.5643309E-1037.49999611
TCGTTCA850.037.05882316
TCGTTTA552.748493E-936.8181838
CGTTTTT14950.036.5719071
TATGGGT2350.036.382984
CGTTAGG1550.036.290322
TCACCGA250.002107098236.00000422