##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549998_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1136476
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28569279069686	33.0	31.0	34.0	30.0	34.0
2	32.44722985791165	34.0	31.0	34.0	31.0	34.0
3	32.4483147906335	34.0	31.0	34.0	30.0	34.0
4	35.993852047909506	37.0	35.0	37.0	35.0	37.0
5	35.86652071843136	37.0	35.0	37.0	35.0	37.0
6	35.817886167415764	37.0	35.0	37.0	35.0	37.0
7	36.018312749235356	37.0	35.0	37.0	35.0	37.0
8	35.97461890968221	37.0	35.0	37.0	35.0	37.0
9	37.51128136449868	39.0	38.0	39.0	35.0	39.0
10	37.338405738440585	39.0	37.0	39.0	34.0	39.0
11	37.251832858766925	39.0	37.0	39.0	34.0	39.0
12	37.13937029906483	39.0	37.0	39.0	34.0	39.0
13	37.08745455249385	39.0	37.0	39.0	33.0	39.0
14	38.21490027066124	40.0	37.0	41.0	33.0	41.0
15	38.3046760336338	40.0	38.0	41.0	34.0	41.0
16	38.33171488003266	40.0	37.0	41.0	34.0	41.0
17	38.35598200049979	40.0	37.0	41.0	34.0	41.0
18	38.31655573896853	40.0	37.0	41.0	34.0	41.0
19	38.276610328770694	40.0	37.0	41.0	34.0	41.0
20	38.22695067911685	40.0	37.0	41.0	34.0	41.0
21	38.206894822239974	40.0	37.0	41.0	34.0	41.0
22	38.10401187530577	40.0	37.0	41.0	34.0	41.0
23	37.85757640284529	40.0	36.0	41.0	33.0	41.0
24	37.65751498491829	40.0	36.0	41.0	33.0	41.0
25	37.66488689598373	39.0	36.0	41.0	33.0	41.0
26	37.758201669018966	40.0	36.0	41.0	33.0	41.0
27	37.82525455882922	40.0	36.0	41.0	33.0	41.0
28	37.7868542758492	40.0	36.0	41.0	33.0	41.0
29	37.747914606203736	40.0	36.0	41.0	33.0	41.0
30	37.69212988219725	40.0	36.0	41.0	33.0	41.0
31	37.55887057887716	40.0	36.0	41.0	33.0	41.0
32	37.40631918315917	40.0	36.0	41.0	33.0	41.0
33	37.32231652934158	39.0	35.0	41.0	32.0	41.0
34	37.264994597334216	39.0	35.0	41.0	32.0	41.0
35	37.184168429425696	39.0	35.0	41.0	32.0	41.0
36	37.12269066834671	39.0	35.0	41.0	32.0	41.0
37	37.08728736902495	39.0	35.0	41.0	32.0	41.0
38	36.93020530129981	39.0	35.0	41.0	31.0	41.0
39	36.85316715883133	39.0	35.0	41.0	31.0	41.0
40	36.82942270668276	39.0	35.0	41.0	31.0	41.0
41	36.7649215645557	39.0	35.0	41.0	31.0	41.0
42	36.707028569015094	39.0	35.0	41.0	31.0	41.0
43	36.61937251644557	39.0	35.0	41.0	31.0	41.0
44	36.515061470721776	39.0	35.0	41.0	31.0	41.0
45	36.45050401416308	39.0	35.0	40.0	31.0	41.0
46	36.33325208803353	38.0	35.0	40.0	31.0	41.0
47	36.21061245464048	38.0	35.0	40.0	31.0	41.0
48	36.111627522270595	38.0	35.0	40.0	30.0	41.0
49	36.07525191909024	38.0	35.0	40.0	30.0	41.0
50	35.97406984397383	38.0	35.0	40.0	30.0	41.0
51	35.00443564140378	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	9.0
10	13.0
11	12.0
12	17.0
13	18.0
14	35.0
15	40.0
16	79.0
17	145.0
18	267.0
19	508.0
20	840.0
21	1316.0
22	1960.0
23	2835.0
24	3955.0
25	5580.0
26	7476.0
27	8905.0
28	10780.0
29	13092.0
30	16259.0
31	20951.0
32	27468.0
33	39245.0
34	70739.0
35	89346.0
36	92209.0
37	144660.0
38	244623.0
39	332879.0
40	209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62553718688296	21.818850552057413	23.768209799415033	11.787402461644593
2	32.19619244049149	25.080072082472487	24.557227781316985	18.166507695719048
3	32.159852033830894	24.392772042700418	25.723640446432654	17.723735477036033
4	29.889060569690866	25.758748974901362	25.589893671313778	18.762296784093987
5	25.74792604507266	30.24912096691879	23.937417068200297	20.06553591980825
6	26.52189751477374	34.56131057761009	23.93512929441537	14.981662613200807
7	78.28374730306668	6.885495162238358	9.273843002403922	5.556914532291047
8	78.02170921339298	5.9020164086175155	9.761578775090719	6.3146956028987855
9	71.23828395848219	7.761888504464679	12.568501226598713	8.431326310454422
10	39.857242915820486	23.563366054364547	19.647753230160603	16.93163779965437
11	29.163836279868647	24.799027872124004	24.49246618494363	21.54466966306372
12	26.107722468402322	22.35128590484973	30.54943527184032	20.991556354907626
13	24.223300800016894	22.623355002657338	32.10036991542276	21.052974281903005
14	19.879434321534287	27.10246410834897	30.60733354685889	22.41076802325786
15	19.28443715485413	25.126355505967567	34.03969815464647	21.549509184531836
16	21.460198015620215	24.666776949095272	30.979272769508555	22.89375226577596
17	21.70613369749999	24.89889799696606	27.98211312865384	25.41285517688011
18	21.574850678764886	25.47383314737839	29.83336207715781	23.117954096698917
19	23.50010031008134	26.284760962835996	27.478714904670227	22.73642382241244
20	24.615038065036128	25.894783523805167	27.565122360700972	21.92505605045773
21	23.642646215142246	26.287224719219765	28.41274254801685	21.657386517621138
22	21.650347213667512	25.013638651410147	26.814644567945123	26.521369566977214
23	22.434877639299025	25.438020688514317	27.652409729725925	24.47469194246073
24	22.95182652339337	24.204030705443845	28.000151345035	24.843991426127783
25	22.549530302443692	25.71897690756338	25.96271280695765	25.768779983035277
26	21.311668702198727	26.15400589189741	26.776456343996706	25.757869061907158
27	24.242218929392262	25.51457311900999	26.54204752234099	23.701160429256753
28	21.8045079702519	25.770187843826005	27.190191433871018	25.235112752051077
29	23.080117837948183	25.702786508470044	25.741942636712086	25.47515301686969
30	24.99938406090406	25.760332818290927	24.781869568737044	24.45841355206797
31	24.911128787585486	27.50792801607777	22.75032644772085	24.830616748615896
32	24.132757752913392	29.003428141025417	22.2199148948152	24.643899211245994
33	24.616093960629172	28.80527173473087	22.737919674502585	23.84071463013737
34	21.836008855444376	27.352271407403233	24.84654317381097	25.96517656334142
35	22.20557231300969	27.974721859502534	23.811149553532147	26.00855627395563
36	23.946392180741167	29.59358578623746	23.029258866883243	23.43076316613813
37	23.599178513228612	29.264322343806644	24.769110830321097	22.367388312643648
38	22.538707372614997	31.172149697837874	23.522010143636997	22.76713278591013
39	22.912670395151327	28.256733974144638	24.676104026833826	24.15449160387021
40	21.912033338143523	26.09865936456203	27.39037164005223	24.59893565724221
41	20.685170650326096	27.47149961811776	24.891858693012434	26.951471038543705
42	22.478697306410343	27.43762296784094	25.59279738419465	24.49088234155407
43	23.932049598935656	24.96902706260405	26.936688500241097	24.162234838219195
44	22.673334060728077	25.343606024236326	25.80881602427152	26.17424389076408
45	22.72260918840345	24.12043897099455	25.97503158887649	27.18192025172551
46	23.23075894255576	25.458082704782154	26.43733787603082	24.87382047663127
47	21.64673957039128	25.742558575808022	27.635955356734325	24.97474649706637
48	21.386725280604253	25.52900369211492	27.483202460940664	25.601068566340164
49	22.325680436718418	24.8475110781046	28.688859245597797	24.13794923957919
50	20.88235915232702	23.77788884235127	28.55203277499921	26.787719230322505
51	20.684730693828996	24.15396365607369	26.024570690450126	29.136734959647193
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	784.0
1	1472.0
2	2160.0
3	3032.5
4	3905.0
5	2816.0
6	1727.0
7	1753.5
8	1780.0
9	1942.0
10	2104.0
11	2139.0
12	2174.0
13	2217.5
14	2261.0
15	2315.0
16	2369.0
17	2484.5
18	2600.0
19	2713.5
20	2827.0
21	2859.0
22	2891.0
23	3519.0
24	4147.0
25	5492.0
26	7660.0
27	8483.0
28	10270.0
29	12057.0
30	14157.5
31	16258.0
32	17330.5
33	18403.0
34	20266.5
35	22130.0
36	24395.5
37	26661.0
38	28090.0
39	29519.0
40	35496.5
41	41474.0
42	47988.5
43	54503.0
44	62485.0
45	70467.0
46	83712.0
47	96957.0
48	113182.5
49	129408.0
50	124518.0
51	119628.0
52	103158.0
53	86688.0
54	76292.0
55	65896.0
56	62193.5
57	58491.0
58	55447.0
59	52403.0
60	50826.5
61	49250.0
62	45089.0
63	40928.0
64	36573.0
65	32218.0
66	26851.0
67	21484.0
68	18539.0
69	15594.0
70	13801.0
71	12008.0
72	9644.0
73	7280.0
74	5964.0
75	3545.0
76	2442.0
77	1836.0
78	1230.0
79	998.5
80	767.0
81	580.0
82	393.0
83	262.0
84	131.0
85	87.5
86	44.0
87	37.0
88	30.0
89	24.0
90	18.0
91	14.0
92	10.0
93	9.0
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1136476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.427028342505857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75604983480433	20.82140283892544
2	11.561697434557638	6.804527961884098
3	4.252675128475813	3.7543077461138252
4	2.091545374523596	2.4619186006297156
5	1.2820078930425285	1.886284130193935
6	0.8644500323923334	1.5262917362333583
7	0.6989110813124325	1.4396813339070658
8	0.5303517808340618	1.2485341508881913
9	0.4478721104358127	1.1861590758851317
>10	7.2620826951788775	48.324615488849595
>50	0.1764080381698265	3.3273268661899302
>100	0.06596332265989792	3.6456258817233174
>500	0.00605168097748609	1.1543339259102665
>1k	0.003631008586491654	1.9479607603470301
>5k	3.0258404887430445E-4	0.47102950231913837
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	5290	0.4654739739334574	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	4616	0.40616783812416635	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	4017	0.3534610497713986	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	2116	0.186189589573383	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1878	0.1652476603113484	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1745	0.15354481748844673	No Hit
ACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1392	0.12248388879307615	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.799129942031332E-5	0.0	0.0	0.49046350296882646	0.0
2	8.799129942031332E-5	0.0	0.0	1.5963381540833241	0.0
3	8.799129942031332E-5	0.0	0.0	2.298156758259743	0.0
4	8.799129942031332E-5	0.0	0.0	3.1070607738306837	0.0
5	8.799129942031332E-5	0.0	0.0	4.9186256462960944	0.0
6	8.799129942031332E-5	0.0	0.0	6.036995061928277	0.0
7	8.799129942031332E-5	0.0	0.0	7.163283694508287	0.0
8	8.799129942031332E-5	0.0	0.0	8.959010133078042	0.0
9	8.799129942031332E-5	0.0	0.0	9.613577409465751	0.0
10	8.799129942031332E-5	0.0	0.0	10.911097110717693	0.0
11	8.799129942031332E-5	0.0	0.0	12.81170917819646	0.0
12	8.799129942031332E-5	0.0	0.0	14.225905342479736	0.0
13	8.799129942031332E-5	0.0	0.0	14.828821726107723	0.0
14	8.799129942031332E-5	0.0	0.0	15.086372259510979	0.0
15	8.799129942031332E-5	0.0	0.0	15.540935312316318	0.0
16	8.799129942031332E-5	0.0	0.0	16.45340508730497	0.0
17	8.799129942031332E-5	0.0	0.0	17.53006662701192	0.0
18	1.7598259884062665E-4	0.0	0.0	18.75129787166645	0.0
19	1.7598259884062665E-4	0.0	0.0	19.45082870205794	0.0
20	1.7598259884062665E-4	0.0	0.0	20.185380069618716	0.0
21	2.6397389826093994E-4	0.0	0.0	21.03695986540851	0.0
22	2.6397389826093994E-4	0.0	0.0	21.90763377317251	0.0
23	2.6397389826093994E-4	0.0	0.0	22.726128840380262	0.0
24	2.6397389826093994E-4	0.0	0.0	23.409645254277258	0.0
25	2.6397389826093994E-4	0.0	0.0	24.00613827304756	0.0
26	2.6397389826093994E-4	0.0	0.0	24.557315772616402	0.0
27	2.6397389826093994E-4	0.0	0.0	25.08385570834756	0.0
28	2.6397389826093994E-4	0.0	0.0	25.66028671085003	0.0
29	2.6397389826093994E-4	0.0	0.0	26.234957887364097	0.0
30	2.6397389826093994E-4	0.0	0.0	26.882925816295284	0.0
31	5.279477965218799E-4	0.0	0.0	27.456453105916886	0.0
32	5.279477965218799E-4	0.0	0.0	28.02734065655588	0.0
33	5.279477965218799E-4	0.0	0.0	28.57288671296182	0.0
34	5.279477965218799E-4	0.0	0.0	29.11438516959443	0.0
35	5.279477965218799E-4	0.0	0.0	29.657995417413126	0.0
36	5.279477965218799E-4	0.0	0.0	30.202661560824865	0.0
37	5.279477965218799E-4	0.0	0.0	30.73747267870153	0.0
38	6.159390959421932E-4	0.0	0.0	31.275803448555006	0.0
39	7.039303953625066E-4	0.0	0.0	31.83006064360356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAG	20	7.0332544E-4	45.0	6
TACGTAG	35	1.2119199E-7	45.0	1
CGCATCG	45	1.9283107E-8	40.0	21
CGTAAGG	130	0.0	39.807693	2
TCGTTAG	40	3.4581717E-7	39.375	1
TTTAGCG	35	6.2482286E-6	38.571426	1
CGGTTGA	35	6.2482286E-6	38.571426	30
TGCGTAG	70	0.0	38.571426	1
CGAGGGT	235	0.0	38.29787	4
TATGGGA	565	0.0	38.23009	4
TTACGAG	65	9.094947E-12	38.076927	1
AACGTTG	30	1.1399138E-4	37.499996	2
TGTTACG	30	1.1399138E-4	37.499996	1
ATAACGC	60	1.5643309E-10	37.499996	11
TCGTTCA	85	0.0	37.058823	16
TCGTTTA	55	2.748493E-9	36.81818	38
CGTTTTT	1495	0.0	36.571907	1
TATGGGT	235	0.0	36.38298	4
CGTTAGG	155	0.0	36.29032	2
TCACCGA	25	0.0021070982	36.000004	22
>>END_MODULE