FastQCFastQC Report
Sat 18 Jun 2016
SRR3549996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275258
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT41870.32832571918780357Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC33640.2637897586213927Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC31450.24661676303932226Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30890.24222549476262842No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC20630.16177118669320248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC1350.040.025
AATAGCG403.4586265E-739.3751
TATACGG1100.038.863642
CGGTCTA1450.038.79310631
TAGCGCA700.038.5714324
GTATACG356.248827E-638.571431
TCGACAC659.094947E-1238.0769234
TTACGGG2850.037.8947373
GCGTAAG1550.037.7419361
TAATAGG2100.037.5000042
ATTTACG301.13999224E-437.4999961
TGCAACG301.13999224E-437.4999961
CGAAAGG850.037.0588232
CGTTTTT18700.036.93851
TTATGCG1100.036.8181841
AATACGG552.750312E-936.8181842
TCGAACG552.750312E-936.8181841
CGGGAAC1350.036.6666646
CACGGGC3200.036.56254
TACGGGC2000.036.0000044
Produced by FastQC (version 0.11.5)