##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1275258
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41051849900177	33.0	31.0	34.0	31.0	34.0
2	32.56975451242023	34.0	31.0	34.0	31.0	34.0
3	32.55765186338764	34.0	31.0	34.0	31.0	34.0
4	36.082988697189116	37.0	35.0	37.0	35.0	37.0
5	35.97680626194856	37.0	35.0	37.0	35.0	37.0
6	35.931083749327584	37.0	35.0	37.0	35.0	37.0
7	36.106497665570416	37.0	35.0	37.0	35.0	37.0
8	36.07873151942587	37.0	35.0	37.0	35.0	37.0
9	37.598361272777744	39.0	38.0	39.0	35.0	39.0
10	37.446180302338824	39.0	37.0	39.0	35.0	39.0
11	37.3812679473487	39.0	37.0	39.0	35.0	39.0
12	37.27378303057107	39.0	37.0	39.0	34.0	39.0
13	37.23171232801519	39.0	37.0	39.0	34.0	39.0
14	38.43007532593405	40.0	38.0	41.0	34.0	41.0
15	38.514420611358645	40.0	38.0	41.0	34.0	41.0
16	38.535763743493476	40.0	38.0	41.0	34.0	41.0
17	38.550054185113915	40.0	38.0	41.0	34.0	41.0
18	38.50950552750894	40.0	38.0	41.0	34.0	41.0
19	38.45382110914027	40.0	38.0	41.0	34.0	41.0
20	38.42498067057803	40.0	37.0	41.0	34.0	41.0
21	38.40121606765062	40.0	37.0	41.0	34.0	41.0
22	38.31320877814529	40.0	37.0	41.0	34.0	41.0
23	38.07239397831655	40.0	37.0	41.0	34.0	41.0
24	37.88555649131391	40.0	36.0	41.0	33.0	41.0
25	37.88327930505043	40.0	36.0	41.0	33.0	41.0
26	37.945945055824	40.0	37.0	41.0	34.0	41.0
27	38.01170351411244	40.0	37.0	41.0	34.0	41.0
28	37.99446857028146	40.0	37.0	41.0	34.0	41.0
29	37.97932261550212	40.0	37.0	41.0	34.0	41.0
30	37.91779781032544	40.0	36.0	41.0	34.0	41.0
31	37.79620437589884	40.0	36.0	41.0	33.0	41.0
32	37.68363735024599	40.0	36.0	41.0	33.0	41.0
33	37.60989305693436	40.0	36.0	41.0	33.0	41.0
34	37.54822788800384	40.0	36.0	41.0	33.0	41.0
35	37.46195436531274	40.0	36.0	41.0	33.0	41.0
36	37.3790699607452	40.0	35.0	41.0	33.0	41.0
37	37.33218297787585	40.0	35.0	41.0	33.0	41.0
38	37.24700805640898	40.0	35.0	41.0	33.0	41.0
39	37.18627446367715	39.0	35.0	41.0	32.0	41.0
40	37.125227208925565	39.0	35.0	41.0	32.0	41.0
41	37.03368338014739	39.0	35.0	41.0	32.0	41.0
42	36.97167161468503	39.0	35.0	41.0	32.0	41.0
43	36.896475850376945	39.0	35.0	41.0	32.0	41.0
44	36.81656496175676	39.0	35.0	41.0	32.0	41.0
45	36.75157497541674	39.0	35.0	41.0	31.0	41.0
46	36.64820216771822	39.0	35.0	41.0	31.0	41.0
47	36.53188374430899	39.0	35.0	41.0	31.0	41.0
48	36.46809900427992	39.0	35.0	41.0	31.0	41.0
49	36.41207975170514	39.0	35.0	40.0	31.0	41.0
50	36.33057153924931	38.0	35.0	40.0	31.0	41.0
51	35.36587027879849	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	12.0
10	13.0
11	17.0
12	15.0
13	26.0
14	32.0
15	54.0
16	64.0
17	138.0
18	241.0
19	466.0
20	800.0
21	1319.0
22	2040.0
23	3036.0
24	4239.0
25	5800.0
26	7622.0
27	9420.0
28	10877.0
29	12671.0
30	16267.0
31	20656.0
32	26982.0
33	39116.0
34	73802.0
35	96521.0
36	94833.0
37	148179.0
38	267018.0
39	432709.0
40	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.868188241124535	21.175087707742275	24.44258338312718	11.514140668006004
2	32.7233391203976	24.239957718359737	25.607445709025157	17.429257452217513
3	32.34349441446358	24.374518724838428	26.42908336979654	16.85290349090145
4	29.47003665140701	25.717776324477086	25.746476399285477	19.065710624830427
5	25.354477290085615	30.382165804880266	24.07159962925149	20.191757275782628
6	27.19081158479304	34.89403712817328	23.565349129352647	14.349802157681035
7	79.58938504992716	6.7204440199551785	8.462130800198862	5.228040129918809
8	80.05470265624682	5.650307624025883	8.711100028386413	5.5838896913408895
9	73.46560460706775	8.14462642069291	11.219533615942813	7.17023535629653
10	39.17262232426693	26.143415685296624	18.342484422759945	16.341477567676503
11	30.075090687531464	25.311819255397733	24.36110967349352	20.251980383577283
12	27.530115474672577	22.416875643987336	29.341513638808774	20.711495242531317
13	24.66943943892138	23.330808354074232	30.905981377885887	21.0937708291185
14	20.95607320244217	27.31015998331318	29.597069769411366	22.13669704483328
15	20.24594238969683	25.82065746695963	32.701147532499306	21.232252610844238
16	22.442909591627732	24.44101507302836	30.90841225853906	22.207663076804852
17	22.398055922801504	24.636348095836293	28.404840432288996	24.560755549073207
18	22.749906293471593	25.728597664158936	29.15496315255423	22.36653288981524
19	23.89986967343079	26.724631407919023	26.997046872083924	22.378452046566263
20	25.44285156415408	25.69848611026161	27.056485824829174	21.80217650075514
21	24.138958547995777	26.485385702344153	27.81162713741063	21.56402861224944
22	22.3078780921194	24.958949483163405	27.287027409355595	25.446145015361598
23	23.25380432822221	25.63144085353709	27.58179129242867	23.532963525812033
24	23.08230961891633	24.899353699408277	28.12544598818435	23.892890693491044
25	22.744887701155374	26.26049003417348	26.5005983103027	24.494023954368448
26	21.540582376272095	27.41272746377596	27.345446960536613	23.701243199415334
27	22.684743008865656	26.77481733108124	27.960146103768807	22.580293556284296
28	21.552893610547827	26.279780248388953	28.310663410854904	23.85666273020832
29	22.544144008506514	26.03245774580516	27.653070986420005	23.77032725926832
30	23.75817285600247	26.702910313050378	26.637511781929618	22.90140504901753
31	25.176317262859747	27.375715345443822	24.289594732987364	23.158372658709062
32	25.048343158796104	27.186341901011403	25.259986606631756	22.50532833356074
33	24.317981145775992	27.834916542378092	24.62944753140149	23.217654780444427
34	23.73770640921288	26.398266076354744	26.533768068892726	23.330259445539646
35	23.99781063910205	26.080055957304328	26.1479637845832	23.774169619010426
36	23.096894902835345	29.369429558567756	25.43414744310563	22.099528095491266
37	24.37412664731372	29.281525777528937	25.66335596404806	20.68099161110928
38	24.82556470925883	27.312355617451527	24.523978677255897	23.338100996033745
39	23.83141293761733	26.472368728523954	25.664296950107353	24.031921383751367
40	23.18181889468641	25.485980091871607	28.282355413571214	23.04984559987077
41	21.740698744881428	27.49192712376633	26.47338813008819	24.29398600126406
42	22.841338772232756	27.383243233918158	26.48601302638368	23.28940496746541
43	23.576327300044383	25.83390968729465	27.438526164901532	23.151236847759435
44	23.12465399158445	25.497585586602867	26.598304029459136	24.779456392353545
45	23.217341118424663	24.509471808841816	26.540903879842354	25.732283192891163
46	23.424122804953978	25.806464260565313	26.89808650484843	23.871326429632276
47	22.384960533476363	26.149296848167193	28.420053040247538	23.0456895781089
48	22.20515378064674	25.291744886132843	28.202606845046258	24.300494488174156
49	23.071409863729535	24.8900222543203	28.32015168695276	23.718416194997406
50	21.76563487545265	23.984480003262085	28.687214665581394	25.562670455703866
51	21.630603375944318	24.271559166850942	27.15568143857949	26.94215601862525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	670.0
1	1475.0
2	2280.0
3	4004.0
4	5728.0
5	4013.5
6	2299.0
7	2245.5
8	2192.0
9	2331.5
10	2471.0
11	2558.0
12	2645.0
13	2608.5
14	2572.0
15	2543.5
16	2515.0
17	2585.0
18	2655.0
19	2835.0
20	3015.0
21	3347.5
22	3680.0
23	4366.0
24	5052.0
25	6315.5
26	9335.0
27	11091.0
28	13135.0
29	15179.0
30	18096.5
31	21014.0
32	22846.0
33	24678.0
34	27812.0
35	30946.0
36	33770.5
37	36595.0
38	39685.0
39	42775.0
40	47986.0
41	53197.0
42	57946.0
43	62695.0
44	70399.5
45	78104.0
46	89492.5
47	100881.0
48	114692.0
49	128503.0
50	124428.0
51	120353.0
52	106237.0
53	92121.0
54	82492.0
55	72863.0
56	69035.5
57	65208.0
58	61785.0
59	58362.0
60	57625.0
61	56888.0
62	52663.5
63	48439.0
64	41750.5
65	35062.0
66	29721.5
67	24381.0
68	20797.0
69	17213.0
70	14904.0
71	12595.0
72	10745.5
73	8896.0
74	7064.5
75	4270.0
76	3307.0
77	2509.5
78	1712.0
79	1290.0
80	868.0
81	643.0
82	418.0
83	283.0
84	148.0
85	123.0
86	98.0
87	67.5
88	37.0
89	34.0
90	31.0
91	20.5
92	10.0
93	6.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1275258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.257764189119627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.067087329292	20.081969953587045
2	11.100618376823657	6.273573128913816
3	3.9781759693989596	3.372430753382945
4	1.9483053791050393	2.2021901588457404
5	1.214280098671604	1.7156420343901548
6	0.8651501622575047	1.466832556395069
7	0.6292975664733993	1.2447779566734505
8	0.5748788486266547	1.2995832753443686
9	0.4579312134474756	1.1646101019992554
>10	7.882822066406105	51.499167048091465
>50	0.21158300372814756	3.7178162783684194
>100	0.06201311990731039	3.2818938538716225
>500	0.005050842339982962	0.9908322196936376
>1k	0.0028060235222127565	1.688680680442974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	4187	0.32832571918780357	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	3364	0.2637897586213927	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	3145	0.24661676303932226	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3089	0.24222549476262842	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC	2063	0.16177118669320248	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3463612853242246	0.0
2	0.0	0.0	0.0	1.0661372051773053	0.0
3	0.0	0.0	0.0	1.5437660457726985	0.0
4	0.0	0.0	0.0	2.0861660934493256	0.0
5	0.0	0.0	0.0	3.3394811089207046	0.0
6	0.0	0.0	0.0	4.181428385471802	0.0
7	0.0	0.0	0.0	5.0025955532135455	0.0
8	0.0	0.0	0.0	6.378866080432352	0.0
9	0.0	0.0	0.0	6.888645278053539	0.0
10	0.0	0.0	0.0	7.893618389376894	0.0
11	0.0	0.0	0.0	9.405782986658386	0.0
12	0.0	0.0	0.0	10.547120661074073	0.0
13	0.0	0.0	0.0	11.019574078343362	0.0
14	7.841550494096097E-5	0.0	0.0	11.223532806694802	0.0
15	7.841550494096097E-5	0.0	0.0	11.608396104945038	0.0
16	7.841550494096097E-5	0.0	0.0	12.37631914483187	0.0
17	7.841550494096097E-5	0.0	0.0	13.238576037162677	0.0
18	7.841550494096097E-5	0.0	0.0	14.207478016213189	0.0
19	7.841550494096097E-5	0.0	0.0	14.79418282418146	0.0
20	7.841550494096097E-5	0.0	0.0	15.391395309811818	0.0
21	7.841550494096097E-5	0.0	0.0	16.130226197365552	0.0
22	7.841550494096097E-5	0.0	0.0	16.8849754324223	0.0
23	7.841550494096097E-5	0.0	0.0	17.610710930650896	0.0
24	7.841550494096097E-5	0.0	0.0	18.225723735902854	0.0
25	7.841550494096097E-5	0.0	0.0	18.740207863820498	0.0
26	7.841550494096097E-5	0.0	0.0	19.204192406556164	0.0
27	7.841550494096097E-5	0.0	0.0	19.69679860859528	0.0
28	7.841550494096097E-5	0.0	0.0	20.199990903801428	0.0
29	7.841550494096097E-5	0.0	0.0	20.73643137310254	0.0
30	7.841550494096097E-5	0.0	0.0	21.314275229012484	0.0
31	1.5683100988192193E-4	0.0	0.0	21.856047952649583	0.0
32	2.352465148228829E-4	0.0	0.0	22.3863720125653	0.0
33	2.352465148228829E-4	0.0	0.0	22.916852903490902	0.0
34	2.352465148228829E-4	0.0	0.0	23.433062172517246	0.0
35	3.1366201976384386E-4	0.0	0.0	23.966601268135545	0.0
36	3.1366201976384386E-4	0.0	0.0	24.469244654807106	0.0
37	3.1366201976384386E-4	0.0	0.0	24.985846001358155	0.0
38	3.1366201976384386E-4	0.0	0.0	25.49523312145464	0.0
39	3.1366201976384386E-4	0.0	0.0	26.044220071546306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	135	0.0	40.0	25
AATAGCG	40	3.4586265E-7	39.375	1
TATACGG	110	0.0	38.86364	2
CGGTCTA	145	0.0	38.793106	31
TAGCGCA	70	0.0	38.57143	24
GTATACG	35	6.248827E-6	38.57143	1
TCGACAC	65	9.094947E-12	38.07692	34
TTACGGG	285	0.0	37.894737	3
GCGTAAG	155	0.0	37.741936	1
TAATAGG	210	0.0	37.500004	2
ATTTACG	30	1.13999224E-4	37.499996	1
TGCAACG	30	1.13999224E-4	37.499996	1
CGAAAGG	85	0.0	37.058823	2
CGTTTTT	1870	0.0	36.9385	1
TTATGCG	110	0.0	36.818184	1
AATACGG	55	2.750312E-9	36.818184	2
TCGAACG	55	2.750312E-9	36.818184	1
CGGGAAC	135	0.0	36.666664	6
CACGGGC	320	0.0	36.5625	4
TACGGGC	200	0.0	36.000004	4
>>END_MODULE