SRR3549995_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424396
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	2215	0.5219182084656783	Illumina Paired End PCR Primer 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	2154	0.5075448401964203	RNA PCR Primer, Index 23 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	2096	0.4938783588912242	RNA PCR Primer, Index 1 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	790	0.18614690053629157	No Hit
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	756	0.17813551494359042	RNA PCR Primer, Index 23 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	748	0.17625048303942545	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	732	0.17248041923109547	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	709	0.16706095250662117	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	615	0.14491182763268268	No Hit
ACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	558	0.1314809753155072	RNA PCR Primer, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	456	0.10744681853740373	Illumina Paired End PCR Primer 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	434	0.10226298080095006	Illumina Paired End PCR Primer 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTCG	25	3.8866317E-5	45.000004	24
TATGCGG	25	3.8866317E-5	45.000004	2
CTACGAA	25	3.8866317E-5	45.000004	11
GTCACAC	25	3.8866317E-5	45.000004	9
TTAACGG	25	3.8866317E-5	45.000004	2
GCTACGA	25	3.8866317E-5	45.000004	10
TCACGAC	20	7.028251E-4	45.0	25
GCAACTT	20	7.028251E-4	45.0	9
TTTCGCG	20	7.028251E-4	45.0	1
TTATGAG	40	6.7975634E-9	45.0	1
CACGATC	20	7.028251E-4	45.0	20
TCGATTA	20	7.028251E-4	45.0	41
ACGGGTA	20	7.028251E-4	45.0	5
ACTACGG	20	7.028251E-4	45.0	2
CATACGG	20	7.028251E-4	45.0	2
TATAGCG	40	6.7975634E-9	45.0	1
TTCGGCC	20	7.028251E-4	45.0	20
GGCATAT	20	7.028251E-4	45.0	9
ATTTGGC	20	7.028251E-4	45.0	13
GCCGTTT	20	7.028251E-4	45.0	9