SRR3549994_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522681
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2105	0.40273130264922585	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	1704	0.3260114677977581	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	1585	0.30324423501141234	No Hit
TCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	747	0.14291699908739747	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	735	0.140621143680371	No Hit
ACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	637	0.12187165785632155	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	551	0.10541802743929854	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGAA	30	2.1632204E-6	45.000004	11
CAACGTA	20	7.029753E-4	45.0	24
TGTTGCG	25	3.8878774E-5	45.0	1
CTTATTG	20	7.029753E-4	45.0	40
ACTACGC	20	7.029753E-4	45.0	19
GGACGTA	20	7.029753E-4	45.0	8
CCGACGT	20	7.029753E-4	45.0	15
ATGGTCG	20	7.029753E-4	45.0	44
GCCACGA	20	7.029753E-4	45.0	17
GACCTTA	20	7.029753E-4	45.0	37
TCGAACG	25	3.8878774E-5	45.0	1
GCGTAAG	20	7.029753E-4	45.0	1
ATCGACG	25	3.8878774E-5	45.0	1
CGGACCA	20	7.029753E-4	45.0	22
AGGGCGC	120	0.0	43.125004	6
ACGGGTA	70	0.0	41.785713	5
CGTATGG	65	0.0	41.53846	2
GTTAGCG	60	3.6379788E-12	41.250004	1
CTAGGGT	60	3.6379788E-12	41.250004	4
CGGGTAT	55	6.002665E-11	40.909092	6