##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522681
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37820391405083	33.0	31.0	34.0	31.0	34.0
2	32.53742531295379	34.0	31.0	34.0	31.0	34.0
3	32.53785387262977	34.0	31.0	34.0	31.0	34.0
4	36.04888450125411	37.0	35.0	37.0	35.0	37.0
5	35.942762411489994	37.0	35.0	37.0	35.0	37.0
6	35.89771964161697	37.0	35.0	37.0	35.0	37.0
7	36.08790256389653	37.0	35.0	37.0	35.0	37.0
8	36.0691836894779	37.0	35.0	37.0	35.0	37.0
9	37.61710871449316	39.0	38.0	39.0	35.0	39.0
10	37.4441982777258	39.0	37.0	39.0	35.0	39.0
11	37.32519261270259	39.0	37.0	39.0	34.0	39.0
12	37.10249081179534	39.0	37.0	39.0	34.0	39.0
13	36.968577392329166	39.0	37.0	39.0	33.0	39.0
14	38.10140793332836	40.0	37.0	41.0	33.0	41.0
15	38.222923733596595	40.0	37.0	41.0	33.0	41.0
16	38.297458679385706	40.0	37.0	41.0	34.0	41.0
17	38.30047964245878	40.0	37.0	41.0	34.0	41.0
18	38.24262599941456	40.0	37.0	41.0	34.0	41.0
19	38.18100332707713	40.0	37.0	41.0	34.0	41.0
20	38.14512484670382	40.0	36.0	41.0	34.0	41.0
21	38.11891574401977	40.0	36.0	41.0	34.0	41.0
22	38.01130708787961	40.0	36.0	41.0	34.0	41.0
23	37.79047258270341	40.0	35.0	41.0	33.0	41.0
24	37.57554033913611	39.0	35.0	41.0	33.0	41.0
25	37.585010742690095	39.0	35.0	41.0	33.0	41.0
26	37.65146619065931	40.0	35.0	41.0	33.0	41.0
27	37.70189848110033	40.0	35.0	41.0	33.0	41.0
28	37.65386153313398	40.0	35.0	41.0	33.0	41.0
29	37.653402362052574	40.0	36.0	41.0	33.0	41.0
30	37.58211031202588	40.0	35.0	41.0	33.0	41.0
31	37.44773389505262	40.0	35.0	41.0	33.0	41.0
32	37.35471157359843	39.0	35.0	41.0	33.0	41.0
33	37.26957742868021	39.0	35.0	41.0	33.0	41.0
34	37.21523644440873	39.0	35.0	41.0	33.0	41.0
35	37.14644305035002	39.0	35.0	41.0	32.0	41.0
36	37.04060602929894	39.0	35.0	41.0	32.0	41.0
37	37.00466823932762	39.0	35.0	41.0	32.0	41.0
38	36.92767290182731	39.0	35.0	41.0	32.0	41.0
39	36.88222835725806	39.0	35.0	41.0	32.0	41.0
40	36.81664533434351	39.0	35.0	41.0	32.0	41.0
41	36.69837816947622	39.0	35.0	41.0	31.0	41.0
42	36.65542654123643	39.0	35.0	41.0	31.0	41.0
43	36.571239436673615	39.0	35.0	41.0	31.0	41.0
44	36.49925097717346	39.0	35.0	41.0	31.0	41.0
45	36.44552796064904	39.0	35.0	41.0	31.0	41.0
46	36.353357018908284	38.0	35.0	41.0	31.0	41.0
47	36.23792332225583	38.0	35.0	40.0	31.0	41.0
48	36.13782402650948	38.0	35.0	40.0	31.0	41.0
49	36.11559058010526	38.0	35.0	40.0	31.0	41.0
50	36.02370470707755	38.0	35.0	40.0	31.0	41.0
51	35.0444267153388	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	6.0
11	7.0
12	5.0
13	9.0
14	11.0
15	19.0
16	34.0
17	70.0
18	145.0
19	266.0
20	433.0
21	698.0
22	1003.0
23	1500.0
24	2031.0
25	2612.0
26	3341.0
27	4067.0
28	4778.0
29	5868.0
30	7316.0
31	9474.0
32	12489.0
33	17842.0
34	35474.0
35	48946.0
36	38285.0
37	59198.0
38	104084.0
39	162533.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43640002219327	21.367526273195313	24.898360567918097	11.297713136693318
2	31.497414292847836	25.127946108620748	25.545409150131725	17.829230448399695
3	32.4337406563468	24.76596623944624	25.560332210277398	17.239960893929567
4	28.10375735869488	26.374786915920033	26.523634874808916	18.997820850576165
5	25.958663123396487	30.761592634895855	23.513003151061547	19.76674109064611
6	26.256933005026013	34.64311884304193	24.122170119059234	14.977778032872823
7	79.49303686187177	6.697966828715794	8.394795295792271	5.414201013620162
8	80.67693296676175	5.462413977167718	8.193333983825699	5.66731907224483
9	74.4943856769234	7.53652801613221	10.51540040674905	7.453685900195339
10	42.62542545070511	24.732485014760435	17.114836774246626	15.527252760287825
11	33.37408476680805	24.84746910639568	22.573424325735967	19.2050218010603
12	31.120128720959823	21.497624746260147	27.58336346643555	19.79888306634448
13	25.508101499767545	24.820492805363116	28.96221595963886	20.709189735230478
14	20.194726802772628	29.12522169353774	28.340804429470367	22.339247074219266
15	18.65592971621314	25.49643090144849	35.219187228921655	20.62845215341671
16	22.078284842953924	23.57365199806383	31.98317903271785	22.364884126264396
17	22.137785762252694	24.18607142788814	27.473545049466118	26.202597760393047
18	22.2453083238151	26.326191309804642	28.754058402735126	22.67444196364513
19	24.43038870745254	27.4794760092676	25.83621750168841	22.253917781591447
20	27.278779982436706	26.25387186448331	25.372072066901225	21.09527608617876
21	24.681976195805856	27.38190215446898	26.747863419561835	21.18825823016333
22	22.288164291412926	25.73156475938479	26.183656953285084	25.796613995917205
23	22.813149894486312	26.10043219478037	26.924835607186793	24.16158230354652
24	24.646007794429106	23.849345968190924	27.86345782609278	23.64118841128719
25	23.207845703210943	25.069975759593326	26.098710303225104	25.623468233970627
26	21.347246217099915	27.313217813542103	26.015485544720395	25.32405042463759
27	22.106791714257838	27.5037738123253	26.801816021626955	23.587618451789908
28	20.55575006552754	27.70179134118133	28.086347121858264	23.65611147143286
29	22.356465989771966	26.54487153732391	25.68029065529453	25.418371817609593
30	23.46517283008183	27.168004959047682	24.789307436084343	24.57751477478615
31	24.43019738616862	28.17167641448608	23.210906843753648	24.18721935559165
32	26.51808655757527	27.49516435454895	22.542430277741108	23.44431881013467
33	24.773810412086913	28.51509811912046	23.571164821372882	23.139926647419745
34	22.664684578165268	26.34034908481464	26.576439549170527	24.41852678784957
35	24.24997273671704	25.46964592169985	24.818005628672175	25.462375712910934
36	22.863276070873056	29.77246159703529	24.41355243446768	22.95070989762398
37	23.504202372001277	29.36513858357201	26.37670012875922	20.753958915667493
38	24.47898431356793	28.657632475640014	23.75387664751541	23.109506563276643
39	24.596838224461955	25.700762032673847	25.590943615704415	24.111456127159776
40	24.31770047122432	23.594506018010986	28.534230247512344	23.553563263252347
41	20.650262779783464	26.116311861345636	26.809277551699793	26.424147807171106
42	22.814297822189825	27.172214027293894	25.421432958152295	24.592055192363986
43	23.007914961515723	26.24851486853358	26.356228751379906	24.38734141857079
44	22.602122518323796	25.4346341267427	26.14654062420482	25.816702730728686
45	22.63349920888649	24.115856516689913	26.22287781648845	27.027766457935147
46	22.989356796975592	25.502744503817816	26.584475043095118	24.92342365611147
47	22.134150657858235	25.24656530465045	28.649214339147587	23.970069698343732
48	22.364692804980475	24.16387815895355	27.743308059791726	25.728120976274248
49	22.288355612696844	23.08482611765111	29.451041840051577	25.175776429600464
50	20.74936720485344	23.652093724470564	28.98306997958602	26.615469091089977
51	20.502754069882013	23.975809336861296	26.656029203280777	28.86540738997591
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	335.0
1	689.0
2	1043.0
3	1397.5
4	1752.0
5	1312.0
6	872.0
7	866.5
8	861.0
9	867.0
10	873.0
11	918.0
12	963.0
13	988.5
14	1014.0
15	998.5
16	983.0
17	1030.5
18	1078.0
19	1172.5
20	1267.0
21	1408.0
22	1549.0
23	1728.5
24	1908.0
25	2304.0
26	3037.5
27	3375.0
28	4214.0
29	5053.0
30	6046.0
31	7039.0
32	7605.5
33	8172.0
34	9490.0
35	10808.0
36	11157.5
37	11507.0
38	12713.0
39	13919.0
40	16977.5
41	20036.0
42	23936.0
43	27836.0
44	30406.0
45	32976.0
46	38902.5
47	44829.0
48	49563.0
49	54297.0
50	52927.5
51	51558.0
52	45529.0
53	39500.0
54	35158.0
55	30816.0
56	29176.5
57	27537.0
58	26575.5
59	25614.0
60	25520.5
61	25427.0
62	23071.0
63	20715.0
64	17843.5
65	14972.0
66	12538.0
67	10104.0
68	8475.5
69	6847.0
70	5755.0
71	4663.0
72	3917.5
73	3172.0
74	2594.0
75	1686.0
76	1356.0
77	1058.0
78	760.0
79	534.5
80	309.0
81	247.0
82	185.0
83	108.0
84	31.0
85	24.5
86	18.0
87	16.5
88	15.0
89	16.5
90	18.0
91	10.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	522681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.70736431665999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34095841769361	21.490592067307755
2	10.73878668222714	6.380420965756361
3	3.758108777952942	3.3493051982485778
4	1.8723783187285181	2.224936994123336
5	1.079763772747273	1.6038467886467251
6	0.7867288297218827	1.402298397778052
7	0.6119792781179878	1.2726203958508355
8	0.5481405351353894	1.3027048459196782
9	0.43943500118086565	1.17490101062147
>10	7.580050297132347	51.613307853833476
>50	0.18242680486997223	3.480622208168907
>100	0.05668261437031281	3.143650442366646
>500	0.002606097212428175	0.5167803645560242
>1k	0.0019545729093211313	1.0440124668221702
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2105	0.40273130264922585	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	1704	0.3260114677977581	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	1585	0.30324423501141234	No Hit
TCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	747	0.14291699908739747	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	735	0.140621143680371	No Hit
ACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	637	0.12187165785632155	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	551	0.10541802743929854	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.739638517566163E-4	0.0	0.0	0.3675281864081533	0.0
2	5.739638517566163E-4	0.0	0.0	1.4176907138388424	0.0
3	5.739638517566163E-4	0.0	0.0	2.003707806482348	0.0
4	5.739638517566163E-4	0.0	0.0	2.6637662360024565	0.0
5	5.739638517566163E-4	0.0	0.0	4.253072141516527	0.0
6	5.739638517566163E-4	0.0	0.0	5.149794999244281	0.0
7	5.739638517566163E-4	0.0	0.0	6.103340278295939	0.0
8	5.739638517566163E-4	0.0	0.0	7.611908601996246	0.0
9	5.739638517566163E-4	0.0	0.0	8.146077626697737	0.0
10	5.739638517566163E-4	0.0	0.0	9.352167000522307	0.0
11	5.739638517566163E-4	0.0	0.0	10.927889094878138	0.0
12	5.739638517566163E-4	0.0	0.0	12.235761391747548	0.0
13	5.739638517566163E-4	0.0	0.0	12.70851628431108	0.0
14	5.739638517566163E-4	0.0	0.0	12.920882909461028	0.0
15	5.739638517566163E-4	0.0	0.0	13.327440637788632	0.0
16	5.739638517566163E-4	0.0	0.0	14.060009833913993	0.0
17	5.739638517566163E-4	0.0	0.0	14.962472330159313	0.0
18	7.652851356754885E-4	0.0	0.0	16.055108182619993	0.0
19	7.652851356754885E-4	0.0	0.0	16.668675540147813	0.0
20	7.652851356754885E-4	0.0	0.0	17.26999833550483	0.0
21	7.652851356754885E-4	0.0	0.0	18.00964641913519	0.0
22	7.652851356754885E-4	0.0	0.0	18.716769884499342	0.0
23	7.652851356754885E-4	0.0	0.0	19.419492960333358	0.0
24	7.652851356754885E-4	0.0	0.0	19.9785337519443	0.0
25	7.652851356754885E-4	0.0	0.0	20.49127479284688	0.0
26	7.652851356754885E-4	0.0	0.0	20.95408097864663	0.0
27	7.652851356754885E-4	0.0	0.0	21.373648554280717	0.0
28	7.652851356754885E-4	0.0	0.0	21.837220025216144	0.0
29	7.652851356754885E-4	0.0	0.0	22.29734771304103	0.0
30	7.652851356754885E-4	0.0	0.0	22.826351063076714	0.0
31	9.566064195943606E-4	0.0	0.0	23.338518140127533	0.0
32	9.566064195943606E-4	0.0	0.0	23.81796927762823	0.0
33	9.566064195943606E-4	0.0	0.0	24.303925338782165	0.0
34	9.566064195943606E-4	0.0	0.0	24.787202901961233	0.0
35	9.566064195943606E-4	0.0	0.0	25.273732926966925	0.0
36	9.566064195943606E-4	0.0	0.0	25.755288598590727	0.0
37	9.566064195943606E-4	0.0	0.0	26.20585022221967	0.0
38	9.566064195943606E-4	0.0	0.0	26.685301359720366	0.0
39	9.566064195943606E-4	0.0	0.0	27.167239673912004	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGAA	30	2.1632204E-6	45.000004	11
CAACGTA	20	7.029753E-4	45.0	24
TGTTGCG	25	3.8878774E-5	45.0	1
CTTATTG	20	7.029753E-4	45.0	40
ACTACGC	20	7.029753E-4	45.0	19
GGACGTA	20	7.029753E-4	45.0	8
CCGACGT	20	7.029753E-4	45.0	15
ATGGTCG	20	7.029753E-4	45.0	44
GCCACGA	20	7.029753E-4	45.0	17
GACCTTA	20	7.029753E-4	45.0	37
TCGAACG	25	3.8878774E-5	45.0	1
GCGTAAG	20	7.029753E-4	45.0	1
ATCGACG	25	3.8878774E-5	45.0	1
CGGACCA	20	7.029753E-4	45.0	22
AGGGCGC	120	0.0	43.125004	6
ACGGGTA	70	0.0	41.785713	5
CGTATGG	65	0.0	41.53846	2
GTTAGCG	60	3.6379788E-12	41.250004	1
CTAGGGT	60	3.6379788E-12	41.250004	4
CGGGTAT	55	6.002665E-11	40.909092	6
>>END_MODULE