FastQCFastQC Report
Sat 18 Jun 2016
SRR3549993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644305
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC31320.4861051831042752No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT28240.43830173597907823No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC22960.3563529694787407No Hit
TCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC11470.1780212787422106No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10110.1569132631284873No Hit
ACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC9220.1430999293812713No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCC7790.12090547178742986No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG253.8888895E-545.0000041
AACGAAC253.8888895E-545.00000420
GATTCGC302.1640099E-645.00000411
TCTTCGA207.030977E-445.019
CGATTCG207.030977E-445.010
CCCAATC207.030977E-445.019
CGGTATG207.030977E-445.032
TTCGATC207.030977E-445.021
CGTTTAC351.2108103E-745.033
TAGTACG207.030977E-445.01
TCACGTT207.030977E-445.045
CGTCATA950.042.6315838
CCTCGTG750.042.00000415
CGACGGT700.041.78571328
CGGTCTA700.041.78571331
CCCGATC700.041.78571341
TTACGGG1350.041.6666683
TATGGGT603.6379788E-1241.2500044
TGGCTAG603.6379788E-1241.2500041
GTACTAG501.0804797E-940.5000041