##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549993_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644305
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27608353186767	33.0	31.0	34.0	30.0	34.0
2	32.43381317854122	34.0	31.0	34.0	30.0	34.0
3	32.44273131513802	34.0	31.0	34.0	30.0	34.0
4	35.97607033935791	37.0	35.0	37.0	35.0	37.0
5	35.859890890184	37.0	35.0	37.0	35.0	37.0
6	35.8104313950691	37.0	35.0	37.0	35.0	37.0
7	36.01906705675107	37.0	35.0	37.0	35.0	37.0
8	35.98664762806435	37.0	35.0	37.0	35.0	37.0
9	37.50970270291244	39.0	38.0	39.0	35.0	39.0
10	37.33557554263897	39.0	37.0	39.0	34.0	39.0
11	37.23946578095778	39.0	37.0	39.0	34.0	39.0
12	37.10117878954843	39.0	37.0	39.0	34.0	39.0
13	37.008471143324975	39.0	37.0	39.0	33.0	39.0
14	38.148204654627854	40.0	37.0	41.0	33.0	41.0
15	38.258178968035324	40.0	37.0	41.0	34.0	41.0
16	38.28695726402868	40.0	37.0	41.0	34.0	41.0
17	38.310346807800656	40.0	37.0	41.0	34.0	41.0
18	38.2578359627816	40.0	37.0	41.0	34.0	41.0
19	38.21593034354847	40.0	37.0	41.0	34.0	41.0
20	38.16293525581828	40.0	37.0	41.0	34.0	41.0
21	38.138032453574006	40.0	37.0	41.0	34.0	41.0
22	38.03755364307277	40.0	36.0	41.0	34.0	41.0
23	37.772930522035374	40.0	36.0	41.0	33.0	41.0
24	37.568651492693675	39.0	36.0	41.0	33.0	41.0
25	37.59499305453163	39.0	36.0	41.0	33.0	41.0
26	37.6806776293836	40.0	36.0	41.0	33.0	41.0
27	37.749083120571775	40.0	36.0	41.0	33.0	41.0
28	37.71646502820869	40.0	36.0	41.0	33.0	41.0
29	37.70102047943133	40.0	36.0	41.0	33.0	41.0
30	37.65349795516099	40.0	36.0	41.0	33.0	41.0
31	37.51544842892729	40.0	36.0	41.0	33.0	41.0
32	37.40075274908622	39.0	35.0	41.0	33.0	41.0
33	37.3406337060864	39.0	35.0	41.0	33.0	41.0
34	37.259890890183996	39.0	35.0	41.0	33.0	41.0
35	37.18523214937025	39.0	35.0	41.0	32.0	41.0
36	37.133792225731604	39.0	35.0	41.0	32.0	41.0
37	37.02709431092418	39.0	35.0	41.0	32.0	41.0
38	36.928321214331724	39.0	35.0	41.0	32.0	41.0
39	36.85443229526389	39.0	35.0	41.0	31.0	41.0
40	36.78224443392493	39.0	35.0	41.0	31.0	41.0
41	36.73736817190616	39.0	35.0	41.0	31.0	41.0
42	36.69198283421672	39.0	35.0	41.0	31.0	41.0
43	36.5746176112245	39.0	35.0	41.0	31.0	41.0
44	36.47349003965513	39.0	35.0	40.0	31.0	41.0
45	36.40565260241656	39.0	35.0	40.0	31.0	41.0
46	36.330196102777414	38.0	35.0	40.0	31.0	41.0
47	36.219838430556955	38.0	35.0	40.0	31.0	41.0
48	36.123868354273206	38.0	35.0	40.0	30.0	41.0
49	36.098203490582875	38.0	35.0	40.0	30.0	41.0
50	35.99198516230667	38.0	35.0	40.0	30.0	41.0
51	34.980510782936655	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	7.0
11	10.0
12	8.0
13	8.0
14	17.0
15	22.0
16	33.0
17	111.0
18	154.0
19	299.0
20	519.0
21	803.0
22	1286.0
23	1766.0
24	2481.0
25	3248.0
26	4107.0
27	4964.0
28	6074.0
29	7239.0
30	9346.0
31	11842.0
32	15755.0
33	22068.0
34	40395.0
35	54761.0
36	51240.0
37	80222.0
38	137482.0
39	187906.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.345930886769466	21.235750149385773	24.248298554256138	11.170020409588627
2	31.99540590248407	24.51711534133679	25.249842853927877	18.237635902251263
3	31.786188218312756	24.01595517650802	26.445705062043594	17.75215154313563
4	29.28768207603542	25.785924368117584	25.663156424364235	19.26323713148276
5	25.308200308859934	29.580710998672988	24.366099906100374	20.744988786366704
6	26.688447241601416	34.16968671669473	24.031165364229672	15.11070067747418
7	78.78349539426203	6.846136534715701	8.867849853718347	5.502518217303916
8	78.27147080963208	5.581828481852539	9.61408028806233	6.532620420453046
9	71.86596410085285	7.86552952406081	12.20384755666959	8.064658818416744
10	40.198818882361614	24.55157107270625	18.7462459549437	16.503364089988438
11	30.632697247421643	24.903423068267358	23.552354862991905	20.911524821319098
12	26.816492189258195	21.961648598101828	30.249493640434267	20.97236557220571
13	23.970635025337376	24.57392073629725	31.25507329603216	20.200370942333212
14	19.702470103444796	28.60803501447296	29.12688866297794	22.562606219104307
15	18.619132243269878	25.425691248709846	34.54963099774175	21.405545510278518
16	20.66769619978116	24.572213470328492	31.40313981732254	23.356950512567806
17	20.957310590481214	24.882004640659314	27.288473626620934	26.872211142238534
18	21.577979373122975	25.309286750840055	29.897952056867478	23.21478181916949
19	23.532022877363982	26.353357493733558	26.934448747099587	23.180170881802873
20	25.52967926680687	26.15764273131514	26.636453232552903	21.676224769325085
21	23.911967158411002	26.298259364741856	27.94158046266908	21.84819301417807
22	21.917104476916986	25.08765258689596	26.085316736638703	26.90992619954835
23	21.9898960895849	25.808739649700062	27.007550771761824	25.193813488953214
24	22.72588292811634	24.461241182359288	28.174079046414352	24.63879684311002
25	22.586197530672585	24.835287635514234	26.068554488945452	26.50996034486773
26	20.76563118398895	26.19613381861075	27.293905836521525	25.744329160878777
27	23.201589309410917	25.74743328082197	27.270780142944723	23.78019726682239
28	21.22379928760447	26.174715391002707	27.65724307587245	24.944242245520368
29	21.532193603960856	25.300750420996266	26.845050092735583	26.32200588230729
30	22.62375738198524	25.51214098912782	27.9299400128821	23.934161616004843
31	24.08036566532931	27.66546899372192	23.83203606987374	24.422129271075036
32	23.76933284702121	27.513367116505382	23.50408579787523	25.21321423859818
33	23.943784387828746	25.997935760237777	26.213051272301165	23.845228579632316
34	22.629034385888673	25.65151597457726	26.268149401292867	25.451300238241203
35	21.541505963790442	24.94269018554877	25.84629950101272	27.669504349648072
36	24.040477724059258	25.543492600554085	27.083446504372926	23.332583171013727
37	23.552820480983385	27.886171921683058	26.727093534894188	21.833914062439373
38	22.735040081948764	27.467270935348942	25.395426079263707	24.40226290343859
39	24.00012416479773	25.541474922591007	27.17858778063184	23.27981313197942
40	22.397932656117835	24.39993481348119	29.107953531324448	24.094178999076522
41	20.632619644423063	24.939275653611258	26.298259364741856	28.12984533722383
42	23.716097189995423	23.194760245535885	26.81819945522695	26.270943109241742
43	24.43237286688758	22.841200984006022	28.287069012346638	24.439357136759764
44	23.029310652563613	23.537920705256052	27.37445774904742	26.058310893132912
45	21.86340320189972	23.719822133927256	27.395100146669666	27.021674517503357
46	23.156269158240274	23.64811696323946	28.36451680492934	24.831097073590925
47	21.751965295939037	24.083314579275346	29.545013619326248	24.61970650545937
48	21.118259209535857	23.106137621157682	29.065427088102684	26.71017608120378
49	23.34437882679787	21.89801413926634	30.330666376948805	24.426940656986986
50	21.641148213966986	21.69640154895585	30.012649288768518	26.649800948308645
51	20.440164207944996	22.384584940362096	27.557911237690224	29.617339614002685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	492.0
1	902.0
2	1312.0
3	1762.0
4	2212.0
5	1638.0
6	1064.0
7	1013.0
8	962.0
9	1042.5
10	1123.0
11	1187.5
12	1252.0
13	1267.5
14	1283.0
15	1296.5
16	1310.0
17	1391.5
18	1473.0
19	1368.0
20	1263.0
21	1553.0
22	1843.0
23	2102.0
24	2361.0
25	3037.0
26	3981.0
27	4249.0
28	5506.5
29	6764.0
30	7206.0
31	7648.0
32	8625.0
33	9602.0
34	10909.0
35	12216.0
36	13372.0
37	14528.0
38	16032.0
39	17536.0
40	20011.0
41	22486.0
42	26642.0
43	30798.0
44	36596.0
45	42394.0
46	50290.5
47	58187.0
48	66213.0
49	74239.0
50	71990.5
51	69742.0
52	58862.5
53	47983.0
54	41614.0
55	35245.0
56	33311.5
57	31378.0
58	30335.5
59	29293.0
60	28389.5
61	27486.0
62	25138.5
63	22791.0
64	20192.5
65	17594.0
66	15059.5
67	12525.0
68	11169.5
69	9814.0
70	8113.0
71	6412.0
72	5566.5
73	4721.0
74	3807.0
75	2391.5
76	1890.0
77	1502.5
78	1115.0
79	894.0
80	673.0
81	477.0
82	281.0
83	181.5
84	82.0
85	63.0
86	44.0
87	28.0
88	12.0
89	11.0
90	10.0
91	8.5
92	7.0
93	5.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	644305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.185586792651453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.42083718156125	21.558798795478452
2	11.243830125464825	6.788032202680952
3	4.214248199379562	3.8162866436444065
4	2.0241718531260844	2.4440326062311817
5	1.255386235632917	1.8947285086998704
6	0.8345248811062741	1.5114373937556345
7	0.6781391329611592	1.4329019358844735
8	0.5313778489661694	1.2831961743728593
9	0.43055726950256895	1.1696961444999137
>10	7.1354390308596205	48.93767987217289
>50	0.16110330316406885	3.111531660916153
>100	0.06412849931769274	3.6673927618375046
>500	0.0036495893920638145	0.7459744106385346
>1k	0.002606849565759868	1.6383108891871616
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	3132	0.4861051831042752	No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT	2824	0.43830173597907823	No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	2296	0.3563529694787407	No Hit
TCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	1147	0.1780212787422106	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1011	0.1569132631284873	No Hit
ACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	922	0.1430999293812713	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCC	779	0.12090547178742986	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5520599715973025E-4	0.0	0.0	0.47275746734853835	0.0
2	1.5520599715973025E-4	0.0	0.0	1.7246490404389225	0.0
3	1.5520599715973025E-4	0.0	0.0	2.47413880072326	0.0
4	1.5520599715973025E-4	0.0	0.0	3.30852624145397	0.0
5	1.5520599715973025E-4	0.0	0.0	5.2161631525442145	0.0
6	1.5520599715973025E-4	0.0	0.0	6.386881989120059	0.0
7	1.5520599715973025E-4	0.0	0.0	7.5299741582014725	0.0
8	1.5520599715973025E-4	0.0	0.0	9.423797735544502	0.0
9	1.5520599715973025E-4	0.0	0.0	10.094908467263176	0.0
10	1.5520599715973025E-4	0.0	0.0	11.46196289024608	0.0
11	1.5520599715973025E-4	0.0	0.0	13.442857031995716	0.0
12	1.5520599715973025E-4	0.0	0.0	14.937956402635399	0.0
13	1.5520599715973025E-4	0.0	0.0	15.532860989748643	0.0
14	1.5520599715973025E-4	0.0	0.0	15.772188637368949	0.0
15	1.5520599715973025E-4	0.0	0.0	16.233305654930508	0.0
16	1.5520599715973025E-4	0.0	0.0	17.16376560790309	0.0
17	1.5520599715973025E-4	0.0	0.0	18.225840246467122	0.0
18	1.5520599715973025E-4	0.0	0.0	19.558438938080567	0.0
19	1.5520599715973025E-4	0.0	0.0	20.262453341197105	0.0
20	1.5520599715973025E-4	0.0	0.0	20.946290964682877	0.0
21	1.5520599715973025E-4	0.0	0.0	21.763916157720335	0.0
22	1.5520599715973025E-4	0.0	0.0	22.556087567223596	0.0
23	1.5520599715973025E-4	0.0	0.0	23.3720054942923	0.0
24	1.5520599715973025E-4	0.0	0.0	24.010057348615952	0.0
25	1.5520599715973025E-4	0.0	0.0	24.57454156028589	0.0
26	1.5520599715973025E-4	0.0	0.0	25.078495433063534	0.0
27	1.5520599715973025E-4	0.0	0.0	25.549700840440476	0.0
28	1.5520599715973025E-4	0.0	0.0	26.068399282948292	0.0
29	1.5520599715973025E-4	0.0	0.0	26.59439240732262	0.0
30	1.5520599715973025E-4	0.0	0.0	27.223907931802486	0.0
31	1.5520599715973025E-4	0.0	0.0	27.780631843614437	0.0
32	1.5520599715973025E-4	0.0	0.0	28.324318451664972	0.0
33	1.5520599715973025E-4	0.0	0.0	28.834325358331846	0.0
34	1.5520599715973025E-4	0.0	0.0	29.34371144101008	0.0
35	1.5520599715973025E-4	0.0	0.0	29.880413779188427	0.0
36	1.5520599715973025E-4	0.0	0.0	30.40904540551447	0.0
37	1.5520599715973025E-4	0.0	0.0	30.89794429656762	0.0
38	1.5520599715973025E-4	0.0	0.0	31.38746401160941	0.0
39	1.5520599715973025E-4	0.0	0.0	31.8873825284609	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	25	3.8888895E-5	45.000004	1
AACGAAC	25	3.8888895E-5	45.000004	20
GATTCGC	30	2.1640099E-6	45.000004	11
TCTTCGA	20	7.030977E-4	45.0	19
CGATTCG	20	7.030977E-4	45.0	10
CCCAATC	20	7.030977E-4	45.0	19
CGGTATG	20	7.030977E-4	45.0	32
TTCGATC	20	7.030977E-4	45.0	21
CGTTTAC	35	1.2108103E-7	45.0	33
TAGTACG	20	7.030977E-4	45.0	1
TCACGTT	20	7.030977E-4	45.0	45
CGTCATA	95	0.0	42.63158	38
CCTCGTG	75	0.0	42.000004	15
CGACGGT	70	0.0	41.785713	28
CGGTCTA	70	0.0	41.785713	31
CCCGATC	70	0.0	41.785713	41
TTACGGG	135	0.0	41.666668	3
TATGGGT	60	3.6379788E-12	41.250004	4
TGGCTAG	60	3.6379788E-12	41.250004	1
GTACTAG	50	1.0804797E-9	40.500004	1
>>END_MODULE