FastQCFastQC Report
Sat 18 Jun 2016
SRR3549992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636330
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC38600.6066034918988575No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT36090.5671585498090613No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC33760.5305423286659438No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC11210.17616645451259566No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC10920.17160907076516901No Hit
ACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC10700.16815174516367293No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.14882215202803578No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG9020.14175034966133923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAACG302.163968E-645.0000041
CGACGGT406.8066583E-945.028
TAGGTTG207.030911E-445.01
TAGCGAA253.8888356E-545.018
TTGAGCG207.030911E-445.01
TATTACG207.030911E-445.01
GGACGAT253.8888356E-545.08
TCGTAAG207.030911E-445.01
CGGCTAC253.8888356E-545.025
GATCTCG207.030911E-445.011
CACAACG207.030911E-445.012
TTACACG551.8189894E-1245.034
CGATACG207.030911E-445.010
ATCGACG207.030911E-445.01
GGGCCTA1550.043.5483867
TAGTAAG1000.042.751
TTAATCG1300.041.53846420
ACACGCG556.002665E-1140.90909236
CGTATGG501.0804797E-940.52
ATCGTTG1350.040.023