##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	636330
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27079188471391	33.0	31.0	34.0	30.0	34.0
2	32.4294799867993	34.0	31.0	34.0	30.0	34.0
3	32.43157166878821	34.0	31.0	34.0	30.0	34.0
4	35.97440950450238	37.0	35.0	37.0	35.0	37.0
5	35.858343941036885	37.0	35.0	37.0	35.0	37.0
6	35.81853126522402	37.0	35.0	37.0	35.0	37.0
7	36.04527210723995	37.0	35.0	37.0	35.0	37.0
8	36.01324155705373	37.0	35.0	37.0	35.0	37.0
9	37.53444439206072	39.0	38.0	39.0	35.0	39.0
10	37.357344459635726	39.0	37.0	39.0	34.0	39.0
11	37.28304967548285	39.0	37.0	39.0	34.0	39.0
12	37.14552512061352	39.0	37.0	39.0	34.0	39.0
13	37.02096867977308	39.0	37.0	39.0	33.0	39.0
14	38.12875080539971	40.0	37.0	41.0	33.0	41.0
15	38.23878176417897	40.0	37.0	41.0	33.0	41.0
16	38.28309682083196	40.0	37.0	41.0	34.0	41.0
17	38.31298382914525	40.0	37.0	41.0	34.0	41.0
18	38.28711360457624	40.0	37.0	41.0	34.0	41.0
19	38.23382521647573	40.0	37.0	41.0	34.0	41.0
20	38.185721245265825	40.0	37.0	41.0	34.0	41.0
21	38.16464727421307	40.0	37.0	41.0	34.0	41.0
22	38.0618232678013	40.0	36.0	41.0	34.0	41.0
23	37.810635990759515	40.0	36.0	41.0	33.0	41.0
24	37.62608866468656	39.0	36.0	41.0	33.0	41.0
25	37.65213961309384	39.0	36.0	41.0	33.0	41.0
26	37.73784828626656	40.0	36.0	41.0	33.0	41.0
27	37.79275061681832	40.0	36.0	41.0	33.0	41.0
28	37.72679741643487	40.0	36.0	41.0	33.0	41.0
29	37.71595555765091	40.0	36.0	41.0	33.0	41.0
30	37.66833561202521	40.0	36.0	41.0	33.0	41.0
31	37.46609306491914	40.0	35.0	41.0	33.0	41.0
32	37.31856740999167	39.0	35.0	41.0	32.0	41.0
33	37.288260808071286	39.0	35.0	41.0	32.0	41.0
34	37.205715587823924	39.0	35.0	41.0	32.0	41.0
35	37.1306209042478	39.0	35.0	41.0	32.0	41.0
36	37.03107192808763	39.0	35.0	41.0	32.0	41.0
37	36.91223421809438	39.0	35.0	41.0	31.0	41.0
38	36.789563591218396	39.0	35.0	41.0	31.0	41.0
39	36.818763848946304	39.0	35.0	41.0	31.0	41.0
40	36.77904232080839	39.0	35.0	41.0	31.0	41.0
41	36.693262929612	39.0	35.0	41.0	31.0	41.0
42	36.62022221174548	39.0	35.0	41.0	31.0	41.0
43	36.52127355303066	39.0	35.0	41.0	31.0	41.0
44	36.396621249980356	39.0	35.0	41.0	31.0	41.0
45	36.355618939858246	39.0	35.0	40.0	31.0	41.0
46	36.247816384580325	39.0	35.0	40.0	30.0	41.0
47	36.10287429478415	38.0	35.0	40.0	30.0	41.0
48	36.016491443119136	38.0	35.0	40.0	30.0	41.0
49	35.996445240677005	38.0	35.0	40.0	30.0	41.0
50	35.91762607452108	38.0	35.0	40.0	30.0	41.0
51	34.885117784797195	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	7.0
11	4.0
12	7.0
13	10.0
14	20.0
15	26.0
16	45.0
17	99.0
18	170.0
19	315.0
20	523.0
21	842.0
22	1348.0
23	1956.0
24	2707.0
25	3479.0
26	4127.0
27	5070.0
28	6148.0
29	7410.0
30	9172.0
31	11767.0
32	15327.0
33	22054.0
34	40373.0
35	53454.0
36	49249.0
37	76748.0
38	136840.0
39	186923.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.136627221724574	21.827039429226975	23.84061728977103	12.19571605927742
2	31.712004777395375	25.304480379677212	25.23957694906731	17.743937893860103
3	31.8782707085946	24.756651423003788	26.743199283390695	16.621878585010922
4	28.402872723272516	26.11663759370138	26.744142190372923	18.73634749265318
5	25.231562239718386	29.982084767337703	24.585356654566027	20.200996338377884
6	26.201027768610626	34.7968821209121	23.894048685430516	15.108041425046753
7	78.42974557226596	6.912608237864001	9.05835022708343	5.599295962786605
8	78.08228434931561	5.984630616189713	9.34326528687945	6.589819747615231
9	71.53819559033835	8.394543711596185	12.398126758128644	7.669133939936826
10	37.48070969465529	29.127339587949646	18.50596388666258	14.885986830732481
11	29.058821680574543	25.358383228827808	25.893011487750066	19.68978360284758
12	27.18715132085553	22.62913896877406	30.827558028067198	19.356151682303206
13	24.580956421982307	25.316423868118743	31.162918611412316	18.939701098486633
14	19.874279069036508	29.070765169016077	29.23671679788789	21.81823896405953
15	17.87233039460657	26.286046548174692	35.556236543931604	20.285386513287133
16	20.46626750271086	25.596467241839925	32.0725095469332	21.86475570851602
17	19.748243835745605	25.115270378577154	28.835195574623228	26.301290211054013
18	21.417974950104504	25.568022881209433	30.63944808511307	22.37455408357299
19	22.48220263071048	27.501453648264267	28.060597488724405	21.95574623230085
20	24.333443339147927	26.492071723791117	28.3123536529788	20.862131284082157
21	22.82699228387786	27.00862759888737	29.420583659422	20.74379645781277
22	20.37449122310751	26.15356811717191	27.294485565665617	26.17745509405497
23	20.018072383826002	27.561956846290446	27.878930743482154	24.541040026401394
24	21.655430358461807	25.313280844844655	28.884383888862697	24.146904907830844
25	21.012367796583533	27.030000157151164	26.30160451338142	25.65602753288388
26	21.575911869627397	27.978250278943317	26.899407540112836	23.546430311316456
27	23.682523219084437	26.584790910376693	27.86745870853174	21.865227162007134
28	19.93383936008046	26.924237423978127	30.04070215139943	23.10122106454198
29	23.39587949648767	25.813807301243063	27.50082504360945	23.289488158659815
30	26.721826725126903	25.255606367765154	26.051577011927773	21.970989895180175
31	25.74780381248723	27.41392045008093	25.17891659987742	21.65935913755441
32	25.310452123897974	29.706284475036536	23.744912231075073	21.238351169990413
33	26.380022944069903	27.744723649678626	24.342400955479075	21.532852450772396
34	24.03957066302076	26.419310734995992	27.53979853220813	22.001320069775115
35	25.1672874137633	27.043672308393447	24.757594329986013	23.031445947857243
36	27.191080099948138	27.17410777426807	25.80170666163783	19.833105464145962
37	24.06047176779344	28.458347084060158	28.15944557069445	19.32173557745195
38	22.192887338330742	30.5074411076014	25.71841654487452	21.58125500919334
39	25.123913692580896	26.446340735153147	26.55917527069288	21.870570301573082
40	26.613863875662	24.83774142347524	27.52612638096585	21.02226831989691
41	22.757217167193122	27.00297015699401	25.194631716247855	25.045180959565005
42	23.882105196988984	27.034714692062295	26.234186664152247	22.848993446796474
43	24.315999559976742	25.36954096145082	27.832885452516777	22.48157402605566
44	23.17272484402747	25.22983357691764	27.280970565587037	24.316471013467854
45	24.25801078056983	24.06110037244826	26.297832885452515	25.383055961529394
46	25.811607184951203	24.571527352160043	27.49359609007905	22.123269372809705
47	22.917039900680464	25.29929439127497	29.492873194726005	22.29079251331856
48	21.88769977841686	24.88457247025914	28.924614586771014	24.303113164552983
49	23.343391007810414	23.306146182012476	30.61430389892037	22.736158911256737
50	21.676645765561894	22.9938868197319	29.63195197460437	25.69751544010183
51	21.45914855499505	23.40232269419955	26.517530212311225	28.62099853849418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	670.0
1	1050.0
2	1430.0
3	1813.5
4	2197.0
5	1674.5
6	1152.0
7	1162.0
8	1172.0
9	1245.0
10	1318.0
11	1391.0
12	1464.0
13	1463.5
14	1463.0
15	1578.0
16	1693.0
17	1708.5
18	1724.0
19	1649.0
20	1574.0
21	1779.0
22	1984.0
23	2107.0
24	2230.0
25	3060.0
26	4364.0
27	4838.0
28	5708.5
29	6579.0
30	7565.0
31	8551.0
32	9461.5
33	10372.0
34	12074.0
35	13776.0
36	15144.0
37	16512.0
38	17689.0
39	18866.0
40	22377.0
41	25888.0
42	29849.0
43	33810.0
44	39656.5
45	45503.0
46	53615.0
47	61727.0
48	69523.0
49	77319.0
50	74905.5
51	72492.0
52	60937.0
53	49382.0
54	42424.5
55	35467.0
56	31420.0
57	27373.0
58	26131.5
59	24890.0
60	24086.0
61	23282.0
62	20408.5
63	17535.0
64	15462.5
65	13390.0
66	10950.5
67	8511.0
68	7127.0
69	5743.0
70	4665.0
71	3587.0
72	3170.5
73	2754.0
74	2294.5
75	1524.0
76	1213.0
77	922.5
78	632.0
79	430.5
80	229.0
81	213.5
82	198.0
83	110.0
84	22.0
85	42.5
86	63.0
87	38.0
88	13.0
89	13.5
90	14.0
91	8.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	636330.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.462942618499195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.68648923793384	21.533184655583394
2	12.160914985610596	7.4091451079020505
3	4.603299566981048	4.206901516941175
4	2.2984851799153154	2.800744885809242
5	1.2912137849721708	1.9667085719911201
6	0.8625321976376579	1.5765161305946387
7	0.6557227008229696	1.3982670106172204
8	0.5059326095324201	1.232975684241094
9	0.416264436534437	1.141257567984341
>10	6.193950186836391	45.16718543369662
>50	0.2536835072656204	4.74696246239412
>100	0.0636818680789808	3.6335657799251506
>500	0.004697842727137927	0.9400744339817727
>1k	0.0031318951514252846	2.246510758338039
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3860	0.6066034918988575	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	3609	0.5671585498090613	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	3376	0.5305423286659438	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC	1121	0.17616645451259566	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1092	0.17160907076516901	No Hit
ACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1070	0.16815174516367293	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	947	0.14882215202803578	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	902	0.14175034966133923	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5715116370436724E-4	0.0	0.0	0.6116323291373973	0.0
2	1.5715116370436724E-4	0.0	0.0	2.201687803498185	0.0
3	1.5715116370436724E-4	0.0	0.0	3.0397749595335752	0.0
4	1.5715116370436724E-4	0.0	0.0	3.9419797903603477	0.0
5	1.5715116370436724E-4	0.0	0.0	6.028947244354344	0.0
6	1.5715116370436724E-4	0.0	0.0	7.188722832492575	0.0
7	1.5715116370436724E-4	0.0	0.0	8.386686153410967	0.0
8	1.5715116370436724E-4	0.0	0.0	10.352332909025192	0.0
9	1.5715116370436724E-4	0.0	0.0	11.035783319975485	0.0
10	1.5715116370436724E-4	0.0	0.0	12.513318561123945	0.0
11	1.5715116370436724E-4	0.0	0.0	14.676975783005673	0.0
12	1.5715116370436724E-4	0.0	0.0	16.377665676614335	0.0
13	1.5715116370436724E-4	0.0	0.0	17.035186145553407	0.0
14	1.5715116370436724E-4	0.0	0.0	17.30517184479751	0.0
15	1.5715116370436724E-4	0.0	0.0	17.815913126836705	0.0
16	1.5715116370436724E-4	0.0	0.0	18.828123772256532	0.0
17	1.5715116370436724E-4	0.0	0.0	20.089576163311488	0.0
18	7.857558185218362E-4	0.0	0.0	21.58109785802964	0.0
19	7.857558185218362E-4	0.0	0.0	22.348781292725473	0.0
20	7.857558185218362E-4	0.0	0.0	23.13233699495545	0.0
21	7.857558185218362E-4	0.0	0.0	24.011283453553972	0.0
22	7.857558185218362E-4	0.0	0.0	24.94208979617494	0.0
23	7.857558185218362E-4	0.0	0.0	25.873839045778134	0.0
24	7.857558185218362E-4	0.0	0.0	26.56058963116622	0.0
25	9.429069822262033E-4	0.0	0.0	27.177093646378452	0.0
26	9.429069822262033E-4	0.0	0.0	27.748023824116416	0.0
27	9.429069822262033E-4	0.0	0.0	28.349441327613032	0.0
28	0.0011000581459305707	0.0	0.0	28.943787028742946	0.0
29	0.0011000581459305707	0.0	0.0	29.546461741549198	0.0
30	0.001257209309634938	0.0	0.0	30.23525529206544	0.0
31	0.001257209309634938	0.0	0.0	30.83808715603539	0.0
32	0.001257209309634938	0.0	0.0	31.42928983389122	0.0
33	0.001257209309634938	0.0	0.0	31.9755472789276	0.0
34	0.001257209309634938	0.0	0.0	32.5331196077507	0.0
35	0.001257209309634938	0.0	0.0	33.12526519258875	0.0
36	0.001257209309634938	0.0	0.0	33.70452438200305	0.0
37	0.001257209309634938	0.0	0.0	34.26476828060912	0.0
38	0.0014143604733393052	0.0	0.0	34.80929706284475	0.0
39	0.0014143604733393052	0.0	0.0	35.40568572910282	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAACG	30	2.163968E-6	45.000004	1
CGACGGT	40	6.8066583E-9	45.0	28
TAGGTTG	20	7.030911E-4	45.0	1
TAGCGAA	25	3.8888356E-5	45.0	18
TTGAGCG	20	7.030911E-4	45.0	1
TATTACG	20	7.030911E-4	45.0	1
GGACGAT	25	3.8888356E-5	45.0	8
TCGTAAG	20	7.030911E-4	45.0	1
CGGCTAC	25	3.8888356E-5	45.0	25
GATCTCG	20	7.030911E-4	45.0	11
CACAACG	20	7.030911E-4	45.0	12
TTACACG	55	1.8189894E-12	45.0	34
CGATACG	20	7.030911E-4	45.0	10
ATCGACG	20	7.030911E-4	45.0	1
GGGCCTA	155	0.0	43.548386	7
TAGTAAG	100	0.0	42.75	1
TTAATCG	130	0.0	41.538464	20
ACACGCG	55	6.002665E-11	40.909092	36
CGTATGG	50	1.0804797E-9	40.5	2
ATCGTTG	135	0.0	40.0	23
>>END_MODULE