FastQCFastQC Report
Sat 18 Jun 2016
SRR3549990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565121
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT33270.5887234769191022Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC26990.4775968332445618No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC24150.42734210903505626No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC12130.21464429741595162No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.18509310395472828No Hit
TCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC9860.17447590869919893No Hit
ACTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC9230.16332785368089311No Hit
CGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG8940.1581962093073873No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7690.13607705252503446No Hit
ACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG5810.10280984072437584No Hit
AGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG5700.10086335492752879No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG207.0302375E-445.0000041
ACCTCGC207.0302375E-445.00000415
CGCTATG207.0302375E-445.00000424
TCGCTAT207.0302375E-445.00000423
GTCGTAA207.0302375E-445.00000427
CGTAATG207.0302375E-445.00000429
ACGTAAG207.0302375E-445.0000041
ACTCACG207.0302375E-445.00000418
TCGCTAG253.888281E-545.023
TATAGCG253.888281E-545.01
TTACCGG453.8380676E-1045.02
ATCGCTA253.888281E-545.022
TAATGCG551.8189894E-1245.024
GCGTAAG253.888281E-545.01
GTACGAC253.888281E-545.012
TATCACG302.163535E-644.9999961
ACGATAG302.163535E-644.9999961
TAGTACG302.163535E-644.9999961
CGGGTCC650.041.538466
TGCGTAG650.041.538461
Produced by FastQC (version 0.11.5)