##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549990_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	565121
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22002898494305	33.0	31.0	34.0	30.0	34.0
2	32.38379391316196	34.0	31.0	34.0	30.0	34.0
3	32.377411209280844	34.0	31.0	34.0	30.0	34.0
4	35.946499953107384	37.0	35.0	37.0	35.0	37.0
5	35.81901044201153	37.0	35.0	37.0	35.0	37.0
6	35.77342197511683	37.0	35.0	37.0	35.0	37.0
7	36.01244158330694	37.0	35.0	37.0	35.0	37.0
8	35.99774207647566	37.0	35.0	37.0	35.0	37.0
9	37.56649107005402	39.0	38.0	39.0	35.0	39.0
10	37.28038597043819	39.0	37.0	39.0	34.0	39.0
11	37.20286982787757	39.0	37.0	39.0	34.0	39.0
12	37.12105018217338	39.0	37.0	39.0	34.0	39.0
13	37.05741956147445	39.0	37.0	39.0	33.0	39.0
14	38.206030212998634	40.0	37.0	41.0	33.0	41.0
15	38.30861709262264	40.0	37.0	41.0	34.0	41.0
16	38.33327729813615	40.0	37.0	41.0	34.0	41.0
17	38.357095206159386	40.0	37.0	41.0	34.0	41.0
18	38.31777796259562	40.0	37.0	41.0	34.0	41.0
19	38.2488794435174	40.0	37.0	41.0	34.0	41.0
20	38.20873582825625	40.0	37.0	41.0	34.0	41.0
21	38.18320678226433	40.0	37.0	41.0	34.0	41.0
22	38.10141544908082	40.0	37.0	41.0	34.0	41.0
23	37.85050281267198	40.0	36.0	41.0	33.0	41.0
24	37.64103616747564	39.0	36.0	41.0	33.0	41.0
25	37.635203788215264	39.0	36.0	41.0	33.0	41.0
26	37.70502423374817	39.0	36.0	41.0	33.0	41.0
27	37.80782168774475	40.0	36.0	41.0	33.0	41.0
28	37.80749078515928	40.0	36.0	41.0	33.0	41.0
29	37.768830038168815	40.0	36.0	41.0	33.0	41.0
30	37.70250618894007	40.0	36.0	41.0	33.0	41.0
31	37.57985104075056	40.0	36.0	41.0	33.0	41.0
32	37.45177758391566	39.0	36.0	41.0	33.0	41.0
33	37.399571065311676	39.0	36.0	41.0	33.0	41.0
34	37.312402122731235	39.0	35.0	41.0	33.0	41.0
35	37.18253966849577	39.0	35.0	41.0	32.0	41.0
36	37.0930632554798	39.0	35.0	41.0	32.0	41.0
37	36.98809812411855	39.0	35.0	41.0	31.0	41.0
38	36.930220253715575	39.0	35.0	41.0	31.0	41.0
39	36.913671585377294	39.0	35.0	41.0	31.0	41.0
40	36.82558956400488	39.0	35.0	41.0	31.0	41.0
41	36.78243066529115	39.0	35.0	41.0	31.0	41.0
42	36.695148472627984	39.0	35.0	41.0	31.0	41.0
43	36.56712279317173	39.0	35.0	41.0	31.0	41.0
44	36.48709568393318	39.0	35.0	41.0	31.0	41.0
45	36.45577318839682	39.0	35.0	40.0	31.0	41.0
46	36.393059185554954	39.0	35.0	40.0	31.0	41.0
47	36.28256249546557	38.0	35.0	40.0	31.0	41.0
48	36.20883492207864	38.0	35.0	40.0	30.0	41.0
49	36.18469495913265	38.0	35.0	40.0	30.0	41.0
50	36.076734009176796	38.0	35.0	40.0	30.0	41.0
51	35.15288407261454	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	8.0
11	6.0
12	5.0
13	9.0
14	10.0
15	12.0
16	39.0
17	60.0
18	109.0
19	189.0
20	419.0
21	626.0
22	975.0
23	1469.0
24	2131.0
25	2943.0
26	3615.0
27	4441.0
28	5163.0
29	6272.0
30	7929.0
31	10369.0
32	13736.0
33	19437.0
34	33978.0
35	45875.0
36	46174.0
37	72413.0
38	125371.0
39	161174.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.87567087402521	22.552161395524145	24.520766349153543	12.051401381297103
2	30.680155223394635	25.857294278570432	26.327812981644637	17.134737516390295
3	30.835166274125363	24.690641473242014	27.985864974049807	16.48832727858282
4	28.683414702338084	25.994963910383795	27.051020931800444	18.270600455477677
5	24.46449521429924	29.981366822326542	25.560189764669865	19.99394819870435
6	25.728649262724268	34.3368942226532	24.673830914087425	15.260625600535107
7	78.60316640153171	6.404292178135303	9.307564220759803	5.684977199573189
8	78.80683959718361	5.293379647898415	9.465406523558672	6.434374231359302
9	72.77096409441518	7.334535435773931	12.100063526218278	7.794436943592611
10	37.74872991801756	25.026852656333777	20.385191843870604	16.83922558177806
11	27.06995493000614	25.414734189669115	25.387306435259	22.128004445065745
12	25.30095324718069	22.149769695339582	31.489008548611714	21.060268508868013
13	22.297525662645697	26.711624590132026	30.973543718955764	20.017306028266514
14	18.601149134433157	29.29301866325973	29.758759628468944	22.34707257383817
15	17.388134576488927	25.8587099046045	34.511016224843885	22.24213929406269
16	18.761114876283134	25.48905455645782	31.14713486138367	24.60269570587538
17	18.701835536106426	26.41151187090906	28.451252032750507	26.435400560234
18	20.038540418777572	25.916219712238618	31.593234015370157	22.452005853613652
19	21.187497898680103	29.01307861502227	27.22478902748261	22.574634458815016
20	22.392903466691205	27.358919594210796	28.133090081593142	22.115086857504853
21	20.40678014089018	28.934865276639872	29.0206876049554	21.63766697751455
22	18.97345878139372	26.76081759481598	26.588995984930662	27.676727638859646
23	19.366295005848304	27.243546072434043	27.63514362410882	25.75501529760883
24	20.3860766101419	25.61964605810083	30.003662932363156	23.99061439939411
25	19.400800890428776	29.20843500772401	26.572008472521812	24.818755629325402
26	19.12811592561593	29.798043250914407	26.859026650929625	24.214814172540038
27	20.199390926898843	28.881425393853704	27.262657023894	23.656526655353456
28	16.978487792879754	28.42984068898519	29.348228078588477	25.243443439546574
29	18.852068848972166	28.1882994969219	27.584534993390797	25.37509666071514
30	21.489911010208434	30.252105301342546	26.528831878482663	21.72915180996636
31	21.899203887309092	31.156867290367902	24.403977201342723	22.539951620980286
32	22.41573043649059	31.325857648185078	23.180168494888704	23.078243420435623
33	23.86674712141294	29.666213076491584	23.623436396807055	22.843603405288427
34	22.174366197681557	27.044119754884356	26.45380369867692	24.32771034875717
35	23.294126390631387	28.80816674659055	24.443614730296698	23.454092132481364
36	24.85043026183773	30.088954400915913	24.706744219379566	20.35387111786679
37	22.662757179435907	29.618081791333182	28.09239083311362	19.626770196117292
38	23.16282707597134	28.680760403524204	26.65818470734586	21.4982278131586
39	26.068753417409724	26.747900007255083	26.028054168930193	21.155292406405
40	26.18041092084704	24.771686063692556	27.94321923977343	21.10468377568698
41	23.47550347624668	26.11405345049998	25.516836217376454	24.893606855876882
42	23.069218804468424	26.77868987349612	26.774442995393905	23.377648326641552
43	23.09363835355614	25.54479483154935	28.702702607052295	22.658864207842214
44	21.34321676242787	26.008235404453206	27.405104393572348	25.243443439546574
45	22.0328035942745	26.217394150987133	26.322327430762616	25.42747482397575
46	24.639325029506956	26.218809777021207	26.904326684019882	22.237538509451955
47	21.97582464640316	25.896931807524403	29.512794605049187	22.614448941023248
48	21.444787930372435	24.663567625340413	29.55367080678297	24.33797363750418
49	23.44506751651416	23.305274445649694	30.45577849699445	22.793879540841697
50	21.685267402910174	23.311467809548752	30.132661854717842	24.870602932823235
51	21.82736086608001	22.663111085944426	27.148168268388538	28.361359779587026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	420.0
1	735.0
2	1050.0
3	1565.5
4	2081.0
5	1527.0
6	973.0
7	1012.5
8	1052.0
9	1045.5
10	1039.0
11	1081.5
12	1124.0
13	1203.0
14	1282.0
15	1244.0
16	1206.0
17	1262.0
18	1318.0
19	1407.0
20	1496.0
21	1431.0
22	1366.0
23	1575.5
24	1785.0
25	2229.0
26	3486.5
27	4300.0
28	5134.0
29	5968.0
30	7677.0
31	9386.0
32	10342.0
33	11298.0
34	13334.5
35	15371.0
36	16776.5
37	18182.0
38	19699.0
39	21216.0
40	24385.5
41	27555.0
42	30843.5
43	34132.0
44	38523.5
45	42915.0
46	49446.0
47	55977.0
48	63863.5
49	71750.0
50	67225.0
51	62700.0
52	50832.5
53	38965.0
54	32125.0
55	25285.0
56	23024.5
57	20764.0
58	19224.0
59	17684.0
60	16898.0
61	16112.0
62	14277.5
63	12443.0
64	11285.5
65	10128.0
66	8877.0
67	7626.0
68	6744.5
69	5863.0
70	4579.5
71	3296.0
72	2969.0
73	2642.0
74	2313.0
75	1656.0
76	1328.0
77	1014.5
78	701.0
79	566.0
80	431.0
81	311.5
82	192.0
83	114.5
84	37.0
85	24.0
86	11.0
87	8.0
88	5.0
89	4.0
90	3.0
91	3.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	565121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.30124762381281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70809225458147	26.393944611885882
2	11.501534740447262	8.350401213337234
3	4.05198209547396	4.4127601624516855
4	1.979122128386199	2.8737840984125946
5	1.2113261210697464	2.198632473707276
6	0.8756708634003227	1.9072766909551846
7	0.6900986348644577	1.7536009000348902
8	0.6038441787494458	1.7536237647185215
9	0.4905223483344431	1.6025915908712507
>10	5.748772372224946	40.2448755853268
>50	0.0818428637222257	2.034059817899862
>100	0.05028898855208117	3.425059633530414
>500	0.0044372636957718675	1.1343485372480413
>1k	0.0024651464976510377	1.9150409196203915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	3327	0.5887234769191022	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	2699	0.4775968332445618	No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	2415	0.42734210903505626	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC	1213	0.21464429741595162	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1046	0.18509310395472828	No Hit
TCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	986	0.17447590869919893	No Hit
ACTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	923	0.16332785368089311	No Hit
CGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG	894	0.1581962093073873	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	769	0.13607705252503446	No Hit
ACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG	581	0.10280984072437584	No Hit
AGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTG	570	0.10086335492752879	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6195133431601374	0.0
2	0.0	0.0	0.0	1.949494002169447	0.0
3	0.0	0.0	0.0	2.8209887793941473	0.0
4	0.0	0.0	0.0	3.7153105264182362	0.0
5	0.0	0.0	0.0	5.864584752645894	0.0
6	0.0	0.0	0.0	7.2048287004022145	0.0
7	0.0	0.0	0.0	8.52489997717303	0.0
8	0.0	0.0	0.0	10.58976750111923	0.0
9	0.0	0.0	0.0	11.315629750088918	0.0
10	0.0	0.0	0.0	12.871933621295263	0.0
11	0.0	0.0	0.0	15.21974939880132	0.0
12	0.0	0.0	0.0	16.882933035579992	0.0
13	0.0	0.0	0.0	17.58012885735975	0.0
14	0.0	0.0	0.0	17.854937261223704	0.0
15	0.0	0.0	0.0	18.3603157553869	0.0
16	0.0	0.0	0.0	19.484853686201717	0.0
17	0.0	0.0	0.0	20.848455463520203	0.0
18	0.0	0.0	0.0	22.412722231168193	0.0
19	0.0	0.0	0.0	23.243340806659106	0.0
20	0.0	0.0	0.0	24.056617963232654	0.0
21	0.0	0.0	0.0	25.13444023492314	0.0
22	0.0	0.0	0.0	26.171740211388357	0.0
23	0.0	0.0	0.0	27.29079259132115	0.0
24	0.0	0.0	0.0	28.156094004646793	0.0
25	0.0	0.0	0.0	28.896820326974222	0.0
26	0.0	0.0	0.0	29.559333310919254	0.0
27	0.0	0.0	0.0	30.24237287235831	0.0
28	0.0	0.0	0.0	30.927712826102727	0.0
29	0.0	0.0	0.0	31.632517637815617	0.0
30	0.0	0.0	0.0	32.39607092994244	0.0
31	0.0	0.0	0.0	33.0782257251102	0.0
32	0.0	0.0	0.0	33.71649611322177	0.0
33	0.0	0.0	0.0	34.35405868831631	0.0
34	0.0	0.0	0.0	34.980827114901054	0.0
35	1.7695325425882245E-4	0.0	0.0	35.70545069109093	0.0
36	3.539065085176449E-4	0.0	0.0	36.31788590408072	0.0
37	3.539065085176449E-4	0.0	0.0	36.91138711886481	0.0
38	3.539065085176449E-4	0.0	0.0	37.5169211549385	0.0
39	3.539065085176449E-4	0.0	0.0	38.09892040819577	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	20	7.0302375E-4	45.000004	1
ACCTCGC	20	7.0302375E-4	45.000004	15
CGCTATG	20	7.0302375E-4	45.000004	24
TCGCTAT	20	7.0302375E-4	45.000004	23
GTCGTAA	20	7.0302375E-4	45.000004	27
CGTAATG	20	7.0302375E-4	45.000004	29
ACGTAAG	20	7.0302375E-4	45.000004	1
ACTCACG	20	7.0302375E-4	45.000004	18
TCGCTAG	25	3.888281E-5	45.0	23
TATAGCG	25	3.888281E-5	45.0	1
TTACCGG	45	3.8380676E-10	45.0	2
ATCGCTA	25	3.888281E-5	45.0	22
TAATGCG	55	1.8189894E-12	45.0	24
GCGTAAG	25	3.888281E-5	45.0	1
GTACGAC	25	3.888281E-5	45.0	12
TATCACG	30	2.163535E-6	44.999996	1
ACGATAG	30	2.163535E-6	44.999996	1
TAGTACG	30	2.163535E-6	44.999996	1
CGGGTCC	65	0.0	41.53846	6
TGCGTAG	65	0.0	41.53846	1
>>END_MODULE