##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	558485
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.188237821964776	33.0	31.0	34.0	30.0	34.0
2	32.3376617098042	34.0	31.0	34.0	30.0	34.0
3	32.3659023966624	34.0	31.0	34.0	30.0	34.0
4	35.91720995192351	37.0	35.0	37.0	35.0	37.0
5	35.7938189924528	37.0	35.0	37.0	35.0	37.0
6	35.72278037906121	37.0	35.0	37.0	33.0	37.0
7	35.91862270248977	37.0	35.0	37.0	35.0	37.0
8	35.77503603498751	37.0	35.0	37.0	35.0	37.0
9	37.394782312864265	39.0	37.0	39.0	35.0	39.0
10	37.28099053689893	39.0	37.0	39.0	34.0	39.0
11	37.17747656606713	39.0	37.0	39.0	34.0	39.0
12	36.99329435884581	39.0	37.0	39.0	33.0	39.0
13	36.80923749071148	39.0	35.0	39.0	33.0	39.0
14	37.894537901644625	40.0	37.0	41.0	33.0	41.0
15	37.94962263982023	40.0	37.0	41.0	33.0	41.0
16	38.08126628289032	40.0	37.0	41.0	33.0	41.0
17	38.04049347789108	40.0	36.0	41.0	33.0	41.0
18	38.01821714101543	40.0	36.0	41.0	33.0	41.0
19	37.95018487515332	40.0	36.0	41.0	34.0	41.0
20	37.86401425284475	40.0	36.0	41.0	33.0	41.0
21	37.90228385722087	40.0	36.0	41.0	33.0	41.0
22	37.80527856612085	40.0	36.0	41.0	33.0	41.0
23	37.51928520909246	39.0	35.0	41.0	33.0	41.0
24	37.31122232468195	39.0	35.0	41.0	32.0	41.0
25	37.32469448597545	39.0	35.0	41.0	32.0	41.0
26	37.39867498679463	39.0	35.0	41.0	33.0	41.0
27	37.46423628208457	39.0	35.0	41.0	33.0	41.0
28	37.44740861437639	39.0	35.0	41.0	33.0	41.0
29	37.39075355649659	39.0	35.0	41.0	33.0	41.0
30	37.33751488401658	39.0	35.0	41.0	33.0	41.0
31	37.18155724862798	39.0	35.0	41.0	32.0	41.0
32	37.04255441059294	39.0	35.0	41.0	32.0	41.0
33	36.95185367556873	39.0	35.0	41.0	32.0	41.0
34	36.89808499780656	39.0	35.0	41.0	32.0	41.0
35	36.87454452671066	39.0	35.0	41.0	31.0	41.0
36	36.74112464972202	39.0	35.0	41.0	31.0	41.0
37	36.71547131973106	39.0	35.0	41.0	31.0	41.0
38	36.51427343617107	39.0	35.0	41.0	31.0	41.0
39	36.464714361173534	39.0	35.0	41.0	31.0	41.0
40	36.327967626704385	39.0	35.0	40.0	30.0	41.0
41	36.30084424827883	39.0	35.0	40.0	30.0	41.0
42	36.28277035193425	39.0	35.0	40.0	30.0	41.0
43	36.17924563775213	38.0	35.0	40.0	30.0	41.0
44	36.08500854991629	38.0	35.0	40.0	30.0	41.0
45	36.00686858196729	38.0	35.0	40.0	30.0	41.0
46	35.83901089554778	38.0	35.0	40.0	30.0	41.0
47	35.65878761291709	38.0	35.0	40.0	29.0	41.0
48	35.62080270732428	38.0	35.0	40.0	29.0	41.0
49	35.651025542315374	38.0	35.0	40.0	29.0	41.0
50	35.56025318495573	38.0	35.0	40.0	29.0	41.0
51	34.432416269013494	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	7.0
11	2.0
12	10.0
13	10.0
14	13.0
15	18.0
16	36.0
17	114.0
18	178.0
19	346.0
20	587.0
21	971.0
22	1460.0
23	2192.0
24	2920.0
25	3541.0
26	4166.0
27	5057.0
28	6041.0
29	7130.0
30	8984.0
31	11582.0
32	15442.0
33	21466.0
34	38321.0
35	51577.0
36	45543.0
37	68506.0
38	115733.0
39	146439.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.985657627331086	20.3289255754407	22.72558797460988	10.959828822618334
2	29.232477147998605	29.89820675577679	24.12616274385167	16.74315335237294
3	30.01835322345273	29.205976883891243	25.11992264787774	15.655747244778285
4	26.520318361280964	24.4676222279918	30.89429438570418	18.117765025023054
5	28.36602594519101	28.67418104335837	23.016553712275172	19.943239299175445
6	24.410324359651558	36.3461865582782	24.17074764765392	15.072741434416322
7	73.1976686929819	7.038685013921592	13.868949031755553	5.894697261340949
8	71.82144551778472	10.341369956220847	10.838428964072445	6.998755561921985
9	66.37761085794605	7.66717100727862	12.70240024351594	13.252817891259388
10	41.562620303141536	22.780379061210237	19.00910498939094	16.647895646257286
11	32.0846576004727	23.539217705041317	24.483916309301055	19.89220838518492
12	27.98427889737415	20.369929362471687	28.976785410530276	22.66900632962389
13	24.011567007171188	24.095186083780227	32.91547669140621	18.977770217642373
14	19.52424863693743	30.293024879808765	27.563318620911932	22.619407862341873
15	16.581824041827446	24.55894070565906	37.86368479010179	20.995550462411703
16	19.118687162591655	24.683384513460524	30.312004798696474	25.885923525251346
17	19.072132644565208	25.266748435499608	29.249129340984986	26.411989578950195
18	19.40338594590723	26.07357404406564	30.160881670949085	24.36215833907804
19	21.48204517578807	27.16617277097863	26.75703017986159	24.594751873371713
20	23.368577490890534	26.571349275271494	29.18771318835779	20.872360045480182
21	21.693868232808402	29.830881760477006	27.486503666168293	20.9887463405463
22	19.770271359123342	24.69627653383708	27.756519870721686	27.776932236317897
23	20.631708998451167	28.58483217991531	26.750047002157622	24.0334118194759
24	22.27275575888341	24.443807801462885	27.103324171642928	26.18011226801078
25	19.544302890856514	30.15192887902092	25.19100781578735	25.112760414335213
26	19.138562360672175	27.673617017466896	27.241376223175195	25.94644439868573
27	21.924134041200748	28.352596757298766	25.064057226246007	24.659211975254482
28	17.76233918547499	27.942558886988905	29.762840541822968	24.532261385713134
29	21.496727754550257	27.23725793888824	25.091452769546187	26.174561537015318
30	21.239424514534857	29.505537301807568	24.88607572271413	24.368962460943443
31	23.221751703268666	29.487273606274115	21.21489386465169	26.076080825805526
32	24.81803450406009	29.684055972855134	21.68778033429725	23.810129188787524
33	21.31606041343993	28.39736071693958	22.07113888466118	28.21543998495931
34	20.90029275629605	28.24480514248368	24.423037324189547	26.431864777030718
35	21.237454900310663	25.323688192162724	24.90541375327896	28.53344315424765
36	20.414335210435375	30.679248323589714	22.99703662587178	25.909379840103135
37	21.790379329793996	26.820057835035854	26.476807792510094	24.912755042660052
38	20.696885323688193	29.767495993625616	24.171105759331045	25.364512923355147
39	23.80941296543327	25.17883201876505	25.04668880990537	25.96506620589631
40	21.002712695954234	25.181875968020627	29.548152591385623	24.267258744639516
41	18.627536997412644	24.613731792259415	25.18921725740172	31.569513952926222
42	20.590347099743056	23.36624976498921	29.513952926220043	26.52945020904769
43	22.8244267974968	23.337779886657653	25.962022256640733	27.875771059204812
44	21.53773154158124	24.401013456046268	26.31870148705874	27.74255351531375
45	21.002891751792795	23.530264913113154	26.434550614609165	29.032292720484882
46	24.23502869369813	25.552521553846567	25.839189951386338	24.373259801068965
47	18.84956623723108	23.256846647627064	32.19585127622049	25.697735838921364
48	20.338594590723115	23.917383636086914	28.328603274931286	27.41541849825868
49	20.997341020797336	21.106206970643797	31.805151436475466	26.091300572083405
50	20.48595754586068	21.56029257724021	29.703931170935654	28.249818705963452
51	19.845295755481345	22.23372158607662	25.959515474900847	31.96146718354119
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	650.0
1	975.5
2	1301.0
3	1739.5
4	2178.0
5	1675.5
6	1173.0
7	1152.5
8	1132.0
9	1186.0
10	1240.0
11	1277.5
12	1315.0
13	1416.0
14	1517.0
15	1475.0
16	1433.0
17	1451.5
18	1470.0
19	1488.0
20	1506.0
21	1492.0
22	1478.0
23	1671.0
24	1864.0
25	2204.5
26	3312.0
27	4079.0
28	4740.0
29	5401.0
30	6608.0
31	7815.0
32	7839.0
33	7863.0
34	8589.0
35	9315.0
36	10757.5
37	12200.0
38	12580.5
39	12961.0
40	15804.0
41	18647.0
42	23392.5
43	28138.0
44	32109.5
45	36081.0
46	55988.0
47	75895.0
48	70572.5
49	65250.0
50	62021.5
51	58793.0
52	49243.0
53	39693.0
54	34732.0
55	29771.0
56	26746.0
57	23721.0
58	22569.5
59	21418.0
60	21539.0
61	21660.0
62	19847.0
63	18034.0
64	15407.0
65	12780.0
66	10691.0
67	8602.0
68	7409.0
69	6216.0
70	5533.5
71	4851.0
72	4256.5
73	3662.0
74	2867.5
75	1711.5
76	1350.0
77	983.5
78	617.0
79	580.5
80	544.0
81	347.0
82	150.0
83	114.5
84	79.0
85	46.0
86	13.0
87	8.5
88	4.0
89	4.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	558485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.128803982590235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.04763060407865	21.707089398803546
2	11.39022268454433	6.863475731613776
3	4.1927504205368535	3.7896766670483286
4	2.139856067491753	2.578852160336616
5	1.2331797199240442	1.8577115028448525
6	0.8647340781871014	1.563204212325902
7	0.6297848682615702	1.3282265392937984
8	0.5221764460375627	1.2586041429593067
9	0.44640445526605954	1.210466909669947
>10	6.2969942020939875	43.04995212847947
>50	0.15991571667116825	3.090085035313435
>100	0.06312462500177693	3.6876343044517124
>500	0.0054106821430094515	1.0172284618007181
>1k	0.006011869047788279	3.218868766855513
>5k	0.001803560714336484	3.778924038203059
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	8048	1.4410413887570839	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	7356	1.3171347484713107	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	5459	0.9774658227168143	RNA PCR Primer, Index 40 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	3723	0.6666248869710019	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	3282	0.587661262164606	RNA PCR Primer, Index 40 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2195	0.39302756564634683	RNA PCR Primer, Index 40 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT	1485	0.26589792026643505	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTA	1464	0.26213774765660675	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTC	1233	0.22077584894849459	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGACTCT	1150	0.20591421434774435	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	1139	0.2039446001235485	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	1082	0.19373841732544295	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1018	0.18227884365739455	No Hit
ACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	905	0.16204553389974663	TruSeq Adapter, Index 19 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	788	0.1410960007878457	Illumina PCR Primer Index 8 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	645	0.11549101587330007	RNA PCR Primer, Index 40 (95% over 24bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	588	0.1052848330751945	No Hit
GCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	579	0.10367333052812519	Illumina PCR Primer Index 8 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	568	0.10170371630392938	RNA PCR Primer, Index 40 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6290231608727181	0.0
2	0.0	0.0	0.0	2.1130379508849835	0.0
3	0.0	0.0	0.0	2.9320393564733163	0.0
4	0.0	0.0	0.0	3.993661423314861	0.0
5	0.0	0.0	0.0	7.026151105222164	0.0
6	0.0	0.0	0.0	8.226362391111667	0.0
7	0.0	0.0	0.0	9.68656275459502	0.0
8	1.7905583856325596E-4	0.0	0.0	11.793870918645979	0.0
9	1.7905583856325596E-4	0.0	0.0	12.46103297313267	0.0
10	1.7905583856325596E-4	0.0	0.0	15.197543353894913	0.0
11	1.7905583856325596E-4	0.0	0.0	17.201178187417746	0.0
12	1.7905583856325596E-4	0.0	0.0	20.25461740243695	0.0
13	1.7905583856325596E-4	0.0	0.0	20.97961449277957	0.0
14	1.7905583856325596E-4	0.0	0.0	21.296006159520847	0.0
15	1.7905583856325596E-4	0.0	0.0	22.04732445813227	0.0
16	1.7905583856325596E-4	0.0	0.0	23.025506504203335	0.0
17	1.7905583856325596E-4	0.0	0.0	24.160541464855815	0.0
18	1.7905583856325596E-4	0.0	0.0	25.503281198241673	0.0
19	1.7905583856325596E-4	0.0	0.0	26.459260320330895	0.0
20	1.7905583856325596E-4	0.0	0.0	27.21953141087048	0.0
21	1.7905583856325596E-4	0.0	0.0	28.030833415400593	0.0
22	1.7905583856325596E-4	0.0	0.0	28.85950383627134	0.0
23	1.7905583856325596E-4	0.0	0.0	29.677968074343983	0.0
24	1.7905583856325596E-4	0.0	0.0	30.341907123736537	0.0
25	3.581116771265119E-4	0.0	0.0	30.908618852789242	0.0
26	3.581116771265119E-4	0.0	0.0	31.432894348102455	0.0
27	3.581116771265119E-4	0.0	0.0	31.94391971136199	0.0
28	3.581116771265119E-4	0.0	0.0	32.47446216102492	0.0
29	3.581116771265119E-4	0.0	0.0	32.99909576801526	0.0
30	3.581116771265119E-4	0.0	0.0	33.61522690851142	0.0
31	3.581116771265119E-4	0.0	0.0	34.142546353080206	0.0
32	3.581116771265119E-4	0.0	0.0	34.68383215305693	0.0
33	3.581116771265119E-4	0.0	0.0	35.15170505922272	0.0
34	3.581116771265119E-4	0.0	0.0	35.633186209119316	0.0
35	3.581116771265119E-4	0.0	0.0	36.130961440325166	0.0
36	3.581116771265119E-4	0.0	0.0	36.63267589997941	0.0
37	3.581116771265119E-4	0.0	0.0	37.119349669194335	0.0
38	3.581116771265119E-4	0.0	0.0	37.608709275987714	0.0
39	5.371675156897679E-4	0.0	0.0	38.13101515707673	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1634896E-6	45.000004	6
TATCGGG	30	2.1634896E-6	45.000004	3
GTTGACG	25	3.8882215E-5	45.0	1
AACGTAG	20	7.030168E-4	45.0	1
GATAAGC	20	7.030168E-4	45.0	9
CAACGAG	20	7.030168E-4	45.0	14
TAACGCC	20	7.030168E-4	45.0	12
ATCGTAC	25	3.8882215E-5	45.0	10
AAAGTCG	20	7.030168E-4	45.0	1
TAGCACG	25	3.8882215E-5	45.0	1
TAACCGG	20	7.030168E-4	45.0	2
TCTACGG	25	3.8882215E-5	45.0	2
TGCGAAT	45	3.8380676E-10	45.0	27
TCGCTCA	20	7.030168E-4	45.0	45
ACTACGG	35	1.2104283E-7	45.0	2
TATAGCG	25	3.8882215E-5	45.0	1
CCGCGAT	20	7.030168E-4	45.0	32
ACCCGAA	20	7.030168E-4	45.0	41
GTCCACT	25	3.8882215E-5	45.0	15
TACAGTC	25	3.8882215E-5	45.0	17
>>END_MODULE