##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549985_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	565811
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27857358729328	33.0	31.0	34.0	30.0	34.0
2	32.45067169072358	34.0	31.0	34.0	31.0	34.0
3	32.44606944721824	34.0	31.0	34.0	30.0	34.0
4	35.981612234474056	37.0	35.0	37.0	35.0	37.0
5	35.86452720077906	37.0	35.0	37.0	35.0	37.0
6	35.81641749630177	37.0	35.0	37.0	35.0	37.0
7	36.00641910461267	37.0	35.0	37.0	35.0	37.0
8	35.97145689991888	37.0	35.0	37.0	35.0	37.0
9	37.48036888643028	39.0	37.0	39.0	35.0	39.0
10	37.32929016933216	39.0	37.0	39.0	34.0	39.0
11	37.25026731541097	39.0	37.0	39.0	34.0	39.0
12	37.1036335454772	39.0	37.0	39.0	34.0	39.0
13	37.034021961397	39.0	37.0	39.0	33.0	39.0
14	38.141927251325974	40.0	37.0	41.0	33.0	41.0
15	38.23728064671772	40.0	37.0	41.0	33.0	41.0
16	38.268946697748895	40.0	37.0	41.0	34.0	41.0
17	38.28824112645388	40.0	37.0	41.0	34.0	41.0
18	38.24453571952472	40.0	37.0	41.0	34.0	41.0
19	38.196705260237074	40.0	37.0	41.0	34.0	41.0
20	38.1414500601791	40.0	37.0	41.0	34.0	41.0
21	38.108492058302154	40.0	37.0	41.0	34.0	41.0
22	38.00851167616042	40.0	36.0	41.0	34.0	41.0
23	37.77236038182361	40.0	36.0	41.0	33.0	41.0
24	37.560111061821	39.0	35.0	41.0	33.0	41.0
25	37.575119607077276	39.0	35.0	41.0	33.0	41.0
26	37.64417623552741	39.0	36.0	41.0	33.0	41.0
27	37.69600803095027	40.0	36.0	41.0	33.0	41.0
28	37.655625288302986	40.0	36.0	41.0	33.0	41.0
29	37.659082273055844	40.0	36.0	41.0	33.0	41.0
30	37.59955002642225	40.0	36.0	41.0	33.0	41.0
31	37.46156402049448	40.0	35.0	41.0	33.0	41.0
32	37.32789924550777	39.0	35.0	41.0	33.0	41.0
33	37.24171322225973	39.0	35.0	41.0	32.0	41.0
34	37.19938813490724	39.0	35.0	41.0	32.0	41.0
35	37.0976916320114	39.0	35.0	41.0	32.0	41.0
36	37.006431476234994	39.0	35.0	41.0	32.0	41.0
37	36.893575770000936	39.0	35.0	41.0	31.0	41.0
38	36.78279319419382	39.0	35.0	41.0	31.0	41.0
39	36.75566399380712	39.0	35.0	41.0	31.0	41.0
40	36.67516538207988	39.0	35.0	41.0	31.0	41.0
41	36.59476220858202	39.0	35.0	41.0	31.0	41.0
42	36.52336204138838	39.0	35.0	41.0	31.0	41.0
43	36.41458720314734	39.0	35.0	41.0	31.0	41.0
44	36.32114787446692	39.0	35.0	40.0	31.0	41.0
45	36.25104672761753	39.0	35.0	40.0	30.0	41.0
46	36.15343639483856	38.0	35.0	40.0	30.0	41.0
47	36.019647903628595	38.0	35.0	40.0	30.0	41.0
48	35.934467516538206	38.0	35.0	40.0	30.0	41.0
49	35.90503896177346	38.0	35.0	40.0	30.0	41.0
50	35.81168446707469	38.0	35.0	40.0	30.0	41.0
51	34.754110471517876	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	12.0
11	7.0
12	5.0
13	11.0
14	10.0
15	23.0
16	42.0
17	90.0
18	161.0
19	318.0
20	501.0
21	834.0
22	1285.0
23	1856.0
24	2475.0
25	3226.0
26	3958.0
27	4784.0
28	5657.0
29	6733.0
30	8404.0
31	10467.0
32	13943.0
33	19390.0
34	36203.0
35	47992.0
36	44135.0
37	68526.0
38	118862.0
39	165803.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.47236798153447	22.423742203668716	25.50728069973896	12.596609115057856
2	31.753005862381606	25.483421142395606	24.785484905737075	17.97808808948571
3	31.09112406793081	24.39189057830265	27.26528823228958	17.251697121476962
4	29.25676595188146	25.41661438183422	26.585379216734918	18.741240449549405
5	25.348747196502014	29.747212408383717	24.74607245175509	20.15796794335918
6	26.474211353261072	34.24217627440965	24.176801087288865	15.106811285040411
7	76.91897117588735	7.223790276258327	9.694226517335295	6.163012030519025
8	76.68815205077314	6.164425930213445	10.313691320953463	6.833730698059953
9	70.38693132512446	8.325571613135835	13.006993501363528	8.280503560376168
10	39.516022134599716	24.599733833382526	19.599477564062912	16.284766467954846
11	29.65053701677769	24.360077835178178	24.673786829877823	21.315598318166312
12	25.942761805620606	21.815588597605913	31.328129004208115	20.913520592565362
13	23.988222215545473	23.567940531378852	31.559831816631352	20.884005436444326
14	19.495555936522972	28.52860760925468	29.857143109624946	22.1186933445974
15	18.7476029981743	25.41272615767456	34.953721295626984	20.885949548524156
16	20.881707849440893	24.97371030255686	31.228272338289642	22.916309509712608
17	20.879763737361063	24.912559140773155	27.23789392571018	26.969783196155607
18	21.336453338659023	25.814803883275513	29.494654575467784	23.354088202597687
19	23.118143691091195	26.335295708284217	27.430007546689616	23.11655305393497
20	24.3277348796683	26.40369310600183	27.12619584985092	22.142376164478954
21	22.77792407712116	26.987103467412265	28.432992642419464	21.80197981304711
22	21.002066060928474	25.79730687455705	27.016265148609698	26.18436191590478
23	21.90201321642739	25.844849251781955	27.244609949258674	25.008527582531975
24	22.469693943737397	25.04016358819465	27.995920899381595	24.494221568686363
25	22.465805719577737	25.890977729312436	26.19832417538719	25.444892375722635
26	21.555431053832464	27.0503754787376	27.182221625242352	24.211971842187584
27	23.27932825625518	25.55128832772781	28.55900645268473	22.61037696333228
28	20.786092882605676	25.645135919945	29.76576984187299	23.80300135557633
29	23.943684375171216	24.21833439081248	27.04896157904318	24.789019654973128
30	24.16425272750088	24.390830153531834	27.958629294941247	23.486287824026046
31	24.319428218963576	26.25452668824042	26.191961626762293	23.234083466033713
32	25.79165127577937	26.517865506326316	24.982193700723386	22.70828951717093
33	24.205255818639085	24.905136167377446	26.683998720420774	24.20560929356269
34	22.753357569930596	23.63598445417286	29.29211344424198	24.318544531654563
35	24.85140797898945	24.062805424426177	26.457598031851624	24.62818856473275
36	25.76726150605061	24.30564269694297	27.967466168031375	21.959629628975048
37	23.704735326814077	25.26408995229856	30.164666293161496	20.866508427725865
38	23.416830001537615	26.74179187043023	26.574421494103156	23.266956633929
39	25.97581171097769	22.700513068851613	27.17462191438484	24.149053305785852
40	23.53471388855996	23.20227072290924	30.020095049406958	23.24292033912384
41	21.66129679345223	24.894708657131094	27.316895571135944	26.12709897828073
42	24.739179690744788	24.60733354424004	28.094363665605655	22.55912309940952
43	25.10325886205818	21.63973482311231	29.911754985321952	23.34525132950756
44	23.58384690294109	22.589875417763174	27.912500817410763	25.913776861884973
45	23.768891025448426	21.79367315234239	27.83685718375924	26.60057863844994
46	24.289559587918934	22.725786525889387	28.672825378085616	24.311828508106064
47	21.419166470782645	23.23726474034616	31.862406351237425	23.481162437633767
48	21.866312249143267	22.489842014382894	30.643448077184782	25.00039765928906
49	24.129612184987568	20.914934492259782	31.32759879182271	23.62785453092994
50	21.74772141227371	20.626498954597913	31.185855347457014	26.439924285671363
51	21.768576432766416	21.573811749859935	27.911440392639946	28.746171424733703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	682.0
1	956.0
2	1230.0
3	1724.0
4	2218.0
5	1658.5
6	1099.0
7	1126.5
8	1154.0
9	1172.0
10	1190.0
11	1289.5
12	1389.0
13	1378.0
14	1367.0
15	1362.5
16	1358.0
17	1421.5
18	1485.0
19	1539.0
20	1593.0
21	1732.5
22	1872.0
23	1912.5
24	1953.0
25	2569.5
26	3808.5
27	4431.0
28	4970.0
29	5509.0
30	6766.5
31	8024.0
32	8716.0
33	9408.0
34	9758.5
35	10109.0
36	11127.0
37	12145.0
38	13486.0
39	14827.0
40	17301.0
41	19775.0
42	24117.5
43	28460.0
44	32193.0
45	35926.0
46	43457.0
47	50988.0
48	59036.0
49	67084.0
50	65665.0
51	64246.0
52	53486.5
53	42727.0
54	37092.5
55	31458.0
56	28667.0
57	25876.0
58	24504.5
59	23133.0
60	22744.0
61	22355.0
62	20709.5
63	19064.0
64	17032.0
65	15000.0
66	13188.0
67	11376.0
68	9533.5
69	7691.0
70	6515.0
71	5339.0
72	4534.0
73	3729.0
74	3006.5
75	1952.5
76	1621.0
77	1202.0
78	783.0
79	547.5
80	312.0
81	250.0
82	188.0
83	139.0
84	90.0
85	70.0
86	50.0
87	33.0
88	16.0
89	10.0
90	4.0
91	4.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	565811.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.20305329234872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15415021604909	23.23583944671564
2	10.977075017141795	7.069906635422539
3	3.9506515952044796	3.81669131579617
4	2.001990207906599	2.57880789423906
5	1.2367748678920754	1.9913963490683024
6	0.9005764085661592	1.7400786047332824
7	0.6988291443018056	1.575310252333825
8	0.5927810845797931	1.527148868593548
9	0.5066056000752779	1.468282242368383
>10	6.797723939608879	45.87110672327207
>50	0.1200421107404035	2.604351558666333
>100	0.054463550243330515	3.3372281139671953
>500	0.006113255639557507	1.38539619403765
>1k	0.0022230020507481843	1.7984558007859894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	3396	0.6002004202816842	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	3048	0.5386957835743738	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	2361	0.41727714731597654	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	1244	0.21986140248245437	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	999	0.17656072434081346	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	959	0.1694912258687088	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG	881	0.15570570384810475	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	848	0.14987336760861844	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	675	0.11929778671676584	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	622	0.10993070124122718	No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG	613	0.10834006408500364	No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG	576	0.10180077799830686	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7673746180261606E-4	0.0	0.0	0.6369618123366283	0.0
2	1.7673746180261606E-4	0.0	0.0	2.00172849237643	0.0
3	1.7673746180261606E-4	0.0	0.0	2.8774626156083922	0.0
4	1.7673746180261606E-4	0.0	0.0	3.8159385377802835	0.0
5	1.7673746180261606E-4	0.0	0.0	5.893664138731838	0.0
6	1.7673746180261606E-4	0.0	0.0	7.1568067782351354	0.0
7	1.7673746180261606E-4	0.0	0.0	8.418888992967617	0.0
8	1.7673746180261606E-4	0.0	0.0	10.405771538552626	0.0
9	1.7673746180261606E-4	0.0	0.0	11.142236541884127	0.0
10	1.7673746180261606E-4	0.0	0.0	12.519905056635519	0.0
11	1.7673746180261606E-4	0.0	0.0	14.67168365408237	0.0
12	1.7673746180261606E-4	0.0	0.0	16.301379789364294	0.0
13	1.7673746180261606E-4	0.0	0.0	16.947355212252855	0.0
14	1.7673746180261606E-4	0.0	0.0	17.203978006790255	0.0
15	1.7673746180261606E-4	0.0	0.0	17.680108728886502	0.0
16	1.7673746180261606E-4	0.0	0.0	18.64156052109273	0.0
17	1.7673746180261606E-4	0.0	0.0	19.806083656910168	0.0
18	1.7673746180261606E-4	0.0	0.0	21.218392714174875	0.0
19	1.7673746180261606E-4	0.0	0.0	21.965108490290927	0.0
20	1.7673746180261606E-4	0.0	0.0	22.68478343475118	0.0
21	1.7673746180261606E-4	0.0	0.0	23.515802980147082	0.0
22	1.7673746180261606E-4	0.0	0.0	24.363082372028824	0.0
23	1.7673746180261606E-4	0.0	0.0	25.128178844172346	0.0
24	1.7673746180261606E-4	0.0	0.0	25.79235822562658	0.0
25	1.7673746180261606E-4	0.0	0.0	26.36728518887049	0.0
26	1.7673746180261606E-4	0.0	0.0	26.877879716018246	0.0
27	1.7673746180261606E-4	0.0	0.0	27.397487853717937	0.0
28	1.7673746180261606E-4	0.0	0.0	27.91904010349746	0.0
29	1.7673746180261606E-4	0.0	0.0	28.47116793416883	0.0
30	1.7673746180261606E-4	0.0	0.0	29.099823085800736	0.0
31	7.069498472104642E-4	0.0	0.0	29.657253040326186	0.0
32	7.069498472104642E-4	0.0	0.0	30.198953360751204	0.0
33	7.069498472104642E-4	0.0	0.0	30.70742703835733	0.0
34	8.836873090130803E-4	0.0	0.0	31.198226969783196	0.0
35	0.0010604247708156963	0.0	0.0	31.70316589815327	0.0
36	0.0010604247708156963	0.0	0.0	32.19590994165896	0.0
37	0.0012371622326183124	0.0	0.0	32.67592888791487	0.0
38	0.0012371622326183124	0.0	0.0	33.16036627071584	0.0
39	0.0012371622326183124	0.0	0.0	33.690225181200084	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	50	2.1827873E-11	45.000004	25
GCGATAA	35	1.2104647E-7	45.000004	9
TACGTAG	25	3.8882856E-5	45.000004	1
GTCGATG	20	7.0302474E-4	45.0	1
GGATATC	40	6.8048394E-9	45.0	8
ACGGGTA	40	6.8048394E-9	45.0	5
ATACCGG	20	7.0302474E-4	45.0	2
CGACCGT	30	2.1635406E-6	44.999996	23
ACTACGG	30	2.1635406E-6	44.999996	2
AAATGCG	30	2.1635406E-6	44.999996	1
TGGGTAC	85	0.0	42.35294	6
ATAGCGG	65	0.0	41.53846	2
AATACGG	60	3.6379788E-12	41.249996	2
CGACGGT	50	1.0786607E-9	40.500004	28
GCGATCA	50	1.0786607E-9	40.500004	9
ACCGTGT	50	1.0786607E-9	40.500004	25
CGGTCTA	50	1.0786607E-9	40.500004	31
TACGGGA	135	0.0	40.000004	4
CGGGATA	45	1.9254003E-8	40.0	6
AAGGGAT	300	0.0	39.75	5
>>END_MODULE