FastQCFastQC Report
Sat 18 Jun 2016
SRR3549984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992883
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC70560.7106577512154No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT63750.6420696094101722No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC58690.5911069078632629No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC29050.2925823082880863No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC20400.20546227501125508No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC18490.18622536592931896No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16920.1704128280975704No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG14580.14684509655216174No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG14370.14473004372116352No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG13900.13999635405178656No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG12620.12710460346284508No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12140.12227019699199201No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG11910.11995371055804158No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCC253.8904203E-545.016
ATTAGCG207.032822E-445.01
GGTCGAC207.032822E-445.09
AGATACG253.8904203E-545.01
GTCCTCG253.8904203E-545.022
CGTGAGA207.032822E-445.018
TTCGTTC207.032822E-445.028
ATTCGGC253.8904203E-545.017
TAACGCA253.8904203E-545.01
CGTTAGG1450.045.02
CACTACG253.8904203E-545.01
CTATTCG207.032822E-445.038
CGGTCTA700.045.031
ACACTCG207.032822E-445.023
TTCGATA207.032822E-445.032
CGTTTAG253.8904203E-545.030
ATGCACG207.032822E-445.01
CCGTTTA207.032822E-445.024
AAGTCGC207.032822E-445.015
CGCATCG302.1652013E-644.99999621