##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549984_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992883
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16800871804634	33.0	31.0	34.0	30.0	34.0
2	32.33123036651851	34.0	31.0	34.0	30.0	34.0
3	32.34662996546421	34.0	31.0	34.0	30.0	34.0
4	35.90382552627047	37.0	35.0	37.0	35.0	37.0
5	35.77318777741184	37.0	35.0	37.0	35.0	37.0
6	35.73000746311499	37.0	35.0	37.0	33.0	37.0
7	35.983256838922614	37.0	35.0	37.0	35.0	37.0
8	35.9494200222987	37.0	35.0	37.0	35.0	37.0
9	37.5181768647464	39.0	37.0	39.0	35.0	39.0
10	37.28018004135432	39.0	37.0	39.0	34.0	39.0
11	37.16594704512012	39.0	37.0	39.0	34.0	39.0
12	36.9603165730504	39.0	35.0	39.0	33.0	39.0
13	36.81488050455089	39.0	35.0	39.0	33.0	39.0
14	37.84292308358588	40.0	36.0	41.0	33.0	41.0
15	37.94857802983836	40.0	36.0	41.0	33.0	41.0
16	37.98308662752812	40.0	36.0	41.0	33.0	41.0
17	38.00011884582574	40.0	36.0	41.0	33.0	41.0
18	37.97397780000262	40.0	36.0	41.0	33.0	41.0
19	37.94518890946869	40.0	36.0	41.0	34.0	41.0
20	37.90211434781339	40.0	36.0	41.0	33.0	41.0
21	37.8924807857522	40.0	36.0	41.0	33.0	41.0
22	37.77398444731151	40.0	36.0	41.0	33.0	41.0
23	37.5289233474639	39.0	35.0	41.0	33.0	41.0
24	37.34017200415356	39.0	35.0	41.0	32.0	41.0
25	37.33439891709295	39.0	35.0	41.0	32.0	41.0
26	37.47240309281154	39.0	35.0	41.0	33.0	41.0
27	37.57781430440445	39.0	35.0	41.0	33.0	41.0
28	37.575367893296594	39.0	35.0	41.0	33.0	41.0
29	37.58702384873142	39.0	35.0	41.0	33.0	41.0
30	37.51000470347463	39.0	35.0	41.0	33.0	41.0
31	37.33284888551823	39.0	35.0	41.0	33.0	41.0
32	37.26770324398746	39.0	35.0	41.0	33.0	41.0
33	37.19627186687656	39.0	35.0	41.0	32.0	41.0
34	37.1158364077137	39.0	35.0	41.0	32.0	41.0
35	37.06467126539582	39.0	35.0	41.0	32.0	41.0
36	36.977137286064924	39.0	35.0	41.0	32.0	41.0
37	36.95879977802017	39.0	35.0	41.0	32.0	41.0
38	36.874946997783226	39.0	35.0	41.0	32.0	41.0
39	36.798484816438595	39.0	35.0	41.0	31.0	41.0
40	36.75621196052304	39.0	35.0	41.0	31.0	41.0
41	36.69519167918073	39.0	35.0	41.0	31.0	41.0
42	36.68802769309173	39.0	35.0	41.0	31.0	41.0
43	36.60701210515237	39.0	35.0	41.0	31.0	41.0
44	36.49591039427606	39.0	35.0	41.0	31.0	41.0
45	36.42788928806315	39.0	35.0	40.0	31.0	41.0
46	36.30172135085403	38.0	35.0	40.0	31.0	41.0
47	36.194296810399614	38.0	35.0	40.0	31.0	41.0
48	36.16952349874054	38.0	35.0	40.0	31.0	41.0
49	36.15308148089956	38.0	35.0	40.0	31.0	41.0
50	36.05758583841198	38.0	35.0	40.0	31.0	41.0
51	35.07426051206436	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	9.0
11	7.0
12	9.0
13	14.0
14	17.0
15	37.0
16	59.0
17	127.0
18	247.0
19	462.0
20	848.0
21	1227.0
22	1836.0
23	2696.0
24	3924.0
25	5076.0
26	6452.0
27	7827.0
28	9329.0
29	11753.0
30	14744.0
31	19394.0
32	25789.0
33	37140.0
34	72671.0
35	93027.0
36	76545.0
37	117518.0
38	208141.0
39	275755.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76804618469648	21.76762015262624	25.67190696184747	11.792426700829806
2	31.213748246268697	24.345063819201254	25.219889956822705	19.221297977707344
3	31.730626871444066	24.193787183384146	27.108027834095257	16.96755811107653
4	29.326617537010907	25.429078753488575	26.15474330812392	19.0895604013766
5	24.535317857189618	29.579114558311502	25.484271560697486	20.401296023801393
6	26.123017515658947	33.669626733462046	24.99710439195756	15.210251358921445
7	78.10557739431533	6.336798998472126	9.70194876939176	5.855674837820771
8	76.69433357203215	5.473555293020426	10.426807589615292	7.405303545332129
9	70.67539679901861	7.165899708223426	13.487591186474138	8.671112306283822
10	39.9764121250943	23.312011586460844	20.558212800501167	16.153363487943693
11	30.63412305377371	23.21069048417588	25.05018214633547	21.105004315714943
12	28.35188033232516	20.622369403041446	30.713790043741305	20.31196022089209
13	24.117242414262304	25.148783894980575	30.90162687849424	19.832346812262873
14	19.105977240017204	28.101095496649652	29.873308335423204	22.919618927909934
15	17.922554822672964	25.543190889561007	35.121862293946016	21.41239199382002
16	20.001551038742733	25.203775268586533	31.82258131119175	22.972092381478987
17	19.827310972189068	25.939511503369477	27.90358984895501	26.329587675486437
18	20.273083535522314	26.652485741018832	30.914720062686136	22.15971066077272
19	21.217504982963753	28.0347231244769	27.493873900550213	23.25389799200913
20	22.90441069088704	28.008738189696068	28.079844251538198	21.00700686787869
21	21.695909789975254	28.481805006229333	29.197700031121492	20.62458517267392
22	19.52395196614304	26.490230973840823	27.16342207490711	26.82239498510902
23	20.394749431705446	26.051407869809434	28.650908515907712	24.902934182577404
24	22.939963721808105	23.585054835262564	29.462484502202173	24.012496940727154
25	20.846565003127257	26.92180246816594	26.01968207734446	26.21195045136235
26	20.510170886197066	26.27107121382882	27.412696158560472	25.80606174141364
27	20.53071711369819	25.539061500700484	27.893618885608877	26.036602499992444
28	17.326512791537372	25.390000634515847	30.58859905950651	26.694887514440268
29	20.753804828967766	24.107372167717646	29.23345449564551	25.90536850766908
30	22.244917074821505	26.972463019308417	26.82451003794002	23.95810986793006
31	21.298682724953494	28.82172421121119	26.155246892131302	23.724346171704017
32	22.26697405434477	27.019195615193333	25.820967828032103	24.892862502429793
33	20.49455978196827	28.98518758000691	25.41810062212768	25.10215201589714
34	18.191468682614165	26.751188206465415	29.891336642887428	25.166006468032993
35	19.56101574908625	26.16400925385972	27.552289645406358	26.72268535164768
36	19.885827433846686	30.875440510110458	25.67160481144304	23.567127244599817
37	18.178576932025223	29.89002732446824	29.793742062257085	22.137653681249454
38	17.657971785195233	29.337696385173277	28.676994167490026	24.32733766214146
39	20.54340743068418	29.636019551145505	28.027471514770625	21.793101503399694
40	21.54010089809172	26.584804050426886	27.772154423028695	24.1029406284527
41	18.19600093868059	26.929859812284025	24.901826297761165	29.972312951274215
42	19.753686990310037	25.61933279147694	26.83226523165368	27.79471498655934
43	21.398190924811885	23.208978298550786	29.383018945837524	26.0098118307998
44	19.730421409169054	23.8913346285514	28.609715344103986	27.76852861817555
45	20.418417880052335	23.920945368185375	27.931488402963893	27.7291483487984
46	21.933097857451482	25.575722416437785	28.118519503305023	24.37266022280571
47	17.237176988628068	25.5996930151891	31.69084373486101	25.472286261321823
48	17.895059135869985	22.911259433387418	31.295731722670244	27.89794970807235
49	20.210135534599747	22.641640555835885	32.60827307950685	24.53995083005752
50	19.23852055075976	21.51159804327398	32.00326725303989	27.24661415292638
51	17.9784526474922	21.75905922450077	28.58866553259548	31.673822595411544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	793.0
1	1296.5
2	1800.0
3	2630.0
4	3460.0
5	2481.0
6	1502.0
7	1515.5
8	1529.0
9	1645.5
10	1762.0
11	1841.0
12	1920.0
13	2022.0
14	2124.0
15	2263.5
16	2403.0
17	2290.5
18	2178.0
19	2243.5
20	2309.0
21	2304.5
22	2300.0
23	3021.5
24	3743.0
25	4239.5
26	5614.0
27	6492.0
28	8123.5
29	9755.0
30	11068.5
31	12382.0
32	13746.5
33	15111.0
34	19101.5
35	23092.0
36	24921.0
37	26750.0
38	29767.0
39	32784.0
40	38988.0
41	45192.0
42	53132.5
43	61073.0
44	77575.5
45	94078.0
46	112439.0
47	130800.0
48	127107.0
49	123414.0
50	102566.5
51	81719.0
52	70364.0
53	59009.0
54	52209.5
55	45410.0
56	42221.0
57	39032.0
58	37431.5
59	35831.0
60	33810.5
61	31790.0
62	29945.5
63	28101.0
64	24288.0
65	20475.0
66	16759.0
67	13043.0
68	11068.5
69	9094.0
70	7249.0
71	5404.0
72	4876.0
73	4348.0
74	3543.0
75	2222.0
76	1706.0
77	1271.0
78	836.0
79	651.0
80	466.0
81	350.5
82	235.0
83	159.0
84	83.0
85	57.0
86	31.0
87	26.0
88	21.0
89	16.0
90	11.0
91	8.0
92	5.0
93	5.5
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	992883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.30415746880278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.23569590780416	19.995895778756346
2	13.029108314982055	7.636140834806436
3	5.274366350848219	4.636825862802327
4	2.6490982336600277	3.105183671580031
5	1.5678552408777309	2.2972338433484376
6	1.0197812612881734	1.793029839871377
7	0.716536044010822	1.4698239546036294
8	0.5459266351158312	1.2798336065478364
9	0.4606556551487343	1.2149213271635722
>10	6.076582076012241	41.23875521946443
>50	0.334023726130848	6.026567335099988
>100	0.07959560343756181	4.509125870408084
>500	0.006256422977622812	1.1565648127055417
>1k	0.0034757905431237845	1.6742943541394713
>5k	0.0010427371629371353	1.965803688702506
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	7056	0.7106577512154	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	6375	0.6420696094101722	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	5869	0.5911069078632629	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2905	0.2925823082880863	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2040	0.20546227501125508	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC	1849	0.18622536592931896	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1692	0.1704128280975704	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1458	0.14684509655216174	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1437	0.14473004372116352	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1390	0.13999635405178656	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1262	0.12710460346284508	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1214	0.12227019699199201	No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1191	0.11995371055804158	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0143360295221089E-4	0.0	0.0	0.6778240739341896	0.0
2	2.0143360295221089E-4	0.0	0.0	2.575932914552873	0.0
3	2.0143360295221089E-4	0.0	0.0	3.672738882627661	0.0
4	3.021504044283163E-4	0.0	0.0	4.79462333427	0.0
5	3.021504044283163E-4	0.0	0.0	7.408828633383792	0.0
6	3.021504044283163E-4	0.0	0.0	8.823899694123075	0.0
7	3.021504044283163E-4	0.0	0.0	10.288120553982695	0.0
8	3.021504044283163E-4	0.0	0.0	12.644490841317658	0.0
9	3.021504044283163E-4	0.0	0.0	13.447808049891075	0.0
10	3.021504044283163E-4	0.0	0.0	15.165130231860148	0.0
11	3.021504044283163E-4	0.0	0.0	17.79122011354812	0.0
12	4.0286720590442177E-4	0.0	0.0	19.69134329019633	0.0
13	4.0286720590442177E-4	0.0	0.0	20.45598524700292	0.0
14	4.0286720590442177E-4	0.0	0.0	20.75471128018105	0.0
15	5.035840073805272E-4	0.0	0.0	21.341588082382316	0.0
16	5.035840073805272E-4	0.0	0.0	22.529542755792978	0.0
17	5.035840073805272E-4	0.0	0.0	23.961836389584676	0.0
18	5.035840073805272E-4	0.0	0.0	25.551751817686476	0.0
19	5.035840073805272E-4	0.0	0.0	26.46998689674413	0.0
20	5.035840073805272E-4	0.0	0.0	27.42065278587709	0.0
21	5.035840073805272E-4	0.0	0.0	28.517055886745972	0.0
22	5.035840073805272E-4	0.0	0.0	29.612653253203046	0.0
23	5.035840073805272E-4	0.0	0.0	30.68911442737966	0.0
24	5.035840073805272E-4	0.0	0.0	31.53372552455828	0.0
25	5.035840073805272E-4	0.0	0.0	32.29524526051912	0.0
26	5.035840073805272E-4	0.0	0.0	32.947890134084275	0.0
27	5.035840073805272E-4	0.0	0.0	33.604563679708484	0.0
28	5.035840073805272E-4	0.0	0.0	34.27191320628916	0.0
29	5.035840073805272E-4	0.0	0.0	34.95779462434144	0.0
30	5.035840073805272E-4	0.0	0.0	35.698969566404095	0.0
31	5.035840073805272E-4	0.0	0.0	36.339427706990655	0.0
32	6.043008088566326E-4	0.0	0.0	36.98552598845987	0.0
33	6.043008088566326E-4	0.0	0.0	37.60835868878811	0.0
34	6.043008088566326E-4	0.0	0.0	38.246802493345136	0.0
35	6.043008088566326E-4	0.0	0.0	38.9036774725723	0.0
36	6.043008088566326E-4	0.0	0.0	39.47766252418462	0.0
37	6.043008088566326E-4	0.0	0.0	40.0902221107623	0.0
38	7.050176103327381E-4	0.0	0.0	40.72967308333409	0.0
39	8.057344118088435E-4	0.0	0.0	41.36761330388374	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCC	25	3.8904203E-5	45.0	16
ATTAGCG	20	7.032822E-4	45.0	1
GGTCGAC	20	7.032822E-4	45.0	9
AGATACG	25	3.8904203E-5	45.0	1
GTCCTCG	25	3.8904203E-5	45.0	22
CGTGAGA	20	7.032822E-4	45.0	18
TTCGTTC	20	7.032822E-4	45.0	28
ATTCGGC	25	3.8904203E-5	45.0	17
TAACGCA	25	3.8904203E-5	45.0	1
CGTTAGG	145	0.0	45.0	2
CACTACG	25	3.8904203E-5	45.0	1
CTATTCG	20	7.032822E-4	45.0	38
CGGTCTA	70	0.0	45.0	31
ACACTCG	20	7.032822E-4	45.0	23
TTCGATA	20	7.032822E-4	45.0	32
CGTTTAG	25	3.8904203E-5	45.0	30
ATGCACG	20	7.032822E-4	45.0	1
CCGTTTA	20	7.032822E-4	45.0	24
AAGTCGC	20	7.032822E-4	45.0	15
CGCATCG	30	2.1652013E-6	44.999996	21
>>END_MODULE