##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549982_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893562
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.257255791987575	33.0	31.0	34.0	30.0	34.0
2	32.41719433010804	34.0	31.0	34.0	30.0	34.0
3	32.42047446064179	34.0	31.0	34.0	30.0	34.0
4	35.97302369617329	37.0	35.0	37.0	35.0	37.0
5	35.86013953144829	37.0	35.0	37.0	35.0	37.0
6	35.81207459583107	37.0	35.0	37.0	35.0	37.0
7	36.032678202519804	37.0	35.0	37.0	35.0	37.0
8	35.9983067766982	37.0	35.0	37.0	35.0	37.0
9	37.52707926254698	39.0	38.0	39.0	35.0	39.0
10	37.369966493651255	39.0	37.0	39.0	34.0	39.0
11	37.282623925368355	39.0	37.0	39.0	34.0	39.0
12	37.157048979253815	39.0	37.0	39.0	34.0	39.0
13	37.05967800779353	39.0	37.0	39.0	33.0	39.0
14	38.161135992801846	40.0	37.0	41.0	33.0	41.0
15	38.26813136637413	40.0	37.0	41.0	34.0	41.0
16	38.30237185556234	40.0	37.0	41.0	34.0	41.0
17	38.34879952370401	40.0	37.0	41.0	34.0	41.0
18	38.303826707044394	40.0	37.0	41.0	34.0	41.0
19	38.27067847558423	40.0	37.0	41.0	34.0	41.0
20	38.21145370998319	40.0	37.0	41.0	34.0	41.0
21	38.18780118223469	40.0	37.0	41.0	34.0	41.0
22	38.07301116206822	40.0	37.0	41.0	34.0	41.0
23	37.83074369769529	40.0	36.0	41.0	33.0	41.0
24	37.63083815112997	39.0	36.0	41.0	33.0	41.0
25	37.635030361631316	39.0	36.0	41.0	33.0	41.0
26	37.724618996779185	39.0	36.0	41.0	33.0	41.0
27	37.7750217668164	40.0	36.0	41.0	33.0	41.0
28	37.74533160541742	40.0	36.0	41.0	33.0	41.0
29	37.75288340372576	40.0	36.0	41.0	33.0	41.0
30	37.6758568515671	40.0	36.0	41.0	33.0	41.0
31	37.50965685649121	40.0	36.0	41.0	33.0	41.0
32	37.4391111081268	39.0	36.0	41.0	33.0	41.0
33	37.359118897177815	39.0	35.0	41.0	33.0	41.0
34	37.326222466935704	39.0	35.0	41.0	33.0	41.0
35	37.27684033116896	39.0	35.0	41.0	33.0	41.0
36	37.17178550565042	39.0	35.0	41.0	32.0	41.0
37	37.096955779229646	39.0	35.0	41.0	32.0	41.0
38	37.00564930021644	39.0	35.0	41.0	32.0	41.0
39	36.97757626219557	39.0	35.0	41.0	32.0	41.0
40	36.945835879323425	39.0	35.0	41.0	32.0	41.0
41	36.88296727031812	39.0	35.0	41.0	32.0	41.0
42	36.851559265053794	39.0	35.0	41.0	32.0	41.0
43	36.75428453761462	39.0	35.0	41.0	31.0	41.0
44	36.66669128723021	39.0	35.0	41.0	31.0	41.0
45	36.62592411047023	39.0	35.0	41.0	31.0	41.0
46	36.520955456924085	39.0	35.0	40.0	31.0	41.0
47	36.42250901448361	39.0	35.0	40.0	31.0	41.0
48	36.374998041546085	38.0	35.0	40.0	31.0	41.0
49	36.36943938976814	39.0	35.0	40.0	31.0	41.0
50	36.28974486381471	38.0	35.0	40.0	31.0	41.0
51	35.31871655240487	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	0.0
10	7.0
11	9.0
12	11.0
13	19.0
14	18.0
15	33.0
16	77.0
17	120.0
18	201.0
19	370.0
20	698.0
21	1042.0
22	1526.0
23	2278.0
24	3248.0
25	4138.0
26	5387.0
27	6473.0
28	7612.0
29	9420.0
30	12024.0
31	15526.0
32	20964.0
33	30014.0
34	55796.0
35	74157.0
36	71694.0
37	110746.0
38	195235.0
39	264575.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.805423686325064	21.63867756238515	23.962411114170028	11.593487637119752
2	31.82957645916008	24.512121151078492	24.99781772277693	18.660484666984495
3	31.527526909156833	24.04947837978786	26.917326385857947	17.50566832519736
4	29.63207925135581	25.680702626118833	25.700063341995293	18.98715478053006
5	25.02557181258827	29.400086395795704	24.756760023367153	20.817581768248875
6	26.358327681794886	33.397682533500756	24.853115956139586	15.390873828564777
7	76.57487672931481	6.777145849980191	10.224696215819383	6.423281204885615
8	74.75776722823933	5.967017397785492	11.112155619867451	8.163059754107717
9	68.31993750853327	7.973257591526944	14.286305818734457	9.420499081205334
10	38.66200666545802	24.119311250926067	20.912370937886795	16.306311145729115
11	28.598575140840815	24.698789787390243	25.5446180567213	21.15801701504764
12	25.93048943442089	21.70918190343815	32.08999487444631	20.270333787694643
13	22.950505952580794	24.585535195095584	32.85077028790392	19.613188564419705
14	18.621427500274184	28.989818277858724	30.160302250990977	22.22845197087611
15	17.386146680364654	25.250290410738145	35.96392863617746	21.399634272719744
16	18.75202839870093	25.409764515500882	32.24107560527417	23.597131480524013
17	18.999688885606147	25.13065685425298	28.192112019087652	27.67754224105322
18	19.9820493709446	25.724795817190078	30.842403772765625	23.450751039099693
19	21.783155505717566	26.2445135312379	28.18539731993975	23.78693364310479
20	23.391102128335806	27.08866312578198	28.623755262645457	20.896479483236753
21	22.154030721986835	27.113731335934162	29.217558490625162	21.514679451453844
22	19.226198070195466	26.27114850452459	26.832049706679562	27.67060371860039
23	20.170172858738397	26.557194688225326	27.878871303837897	25.39376114919838
24	22.136012945939957	24.401216703485602	28.581340746361196	24.881429604213253
25	20.347105181285684	26.90009199137833	26.927622257884735	25.825180569451252
26	19.420140963917447	27.779605668101375	29.10788507120938	23.692368296771797
27	21.701795734375455	28.166372339020683	27.784529780809837	22.347302145794025
28	18.58897312105931	27.956537990648663	29.208157911818095	24.24633097647393
29	21.069271074642835	25.469077691307373	29.450334727752526	24.01131650629727
30	21.629724630187944	27.030692889805074	27.786544190554206	23.553038289452775
31	21.360017547747105	29.51613877940199	24.296243573473358	24.82760009937755
32	22.963487704266743	26.973506035395417	25.305798590360823	24.757207669977014
33	21.11828837842254	27.142828365575077	27.52993077145178	24.208952484550597
34	19.563835525682606	25.02199063970939	28.742381614258438	26.671792220349566
35	20.856862758264118	24.051380877879765	28.20016965806514	26.89158670579098
36	20.830563519934824	26.656012677351992	29.622454849243812	22.890968953469372
37	21.259520883833467	27.903715690685143	28.265190328147348	22.571573097334042
38	21.112468972494355	26.59121583057471	27.170582455386423	25.125732741544514
39	22.719744125197806	22.182456281712966	31.082790002260612	24.01500959082862
40	21.35442196512385	21.58708629059875	31.89504477585215	25.163446968425244
41	18.132037844044397	24.283037998482477	27.951278142982805	29.633646014490324
42	20.307488456313045	24.081485112392873	30.221294101584444	25.389732329709634
43	22.36218639557188	23.84434432081937	30.189511192284364	23.603958091324383
44	21.460402300008283	23.626228510164935	28.75446807272467	26.15890111710211
45	21.73257143880335	22.353904933289463	27.779493756448908	28.134029871458278
46	22.15738807156079	23.100691390188928	29.696092716565836	25.04582782168445
47	18.783699396348545	22.77279024846625	32.302850837434896	26.140659517750308
48	18.764114857167158	22.25307253441843	31.27975451059915	27.70305809781526
49	21.394262513401422	21.159248043224757	32.903592587867436	24.542896855506388
50	20.255897184526646	20.377768974061116	31.590756992799605	27.775576848612634
51	19.09100879401765	20.95847853870241	28.135932369550183	31.814580297729762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	771.0
1	1219.5
2	1668.0
3	2286.0
4	2904.0
5	2154.0
6	1404.0
7	1461.5
8	1519.0
9	1653.5
10	1788.0
11	1884.0
12	1980.0
13	2076.0
14	2172.0
15	2264.0
16	2356.0
17	2229.0
18	2102.0
19	2216.0
20	2330.0
21	2337.5
22	2345.0
23	2732.5
24	3120.0
25	3956.5
26	5426.5
27	6060.0
28	7550.0
29	9040.0
30	10276.0
31	11512.0
32	12757.5
33	14003.0
34	15830.5
35	17658.0
36	19382.0
37	21106.0
38	24195.0
39	27284.0
40	33545.0
41	39806.0
42	48401.5
43	56997.0
44	70959.0
45	84921.0
46	100907.5
47	116894.0
48	113833.0
49	110772.0
50	91735.5
51	72699.0
52	62630.5
53	52562.0
54	44856.5
55	37151.0
56	35943.5
57	34736.0
58	34077.5
59	33419.0
60	31205.0
61	28991.0
62	27371.5
63	25752.0
64	22172.5
65	18593.0
66	16178.5
67	13764.0
68	11844.5
69	9925.0
70	8197.0
71	6469.0
72	5465.0
73	4461.0
74	3999.0
75	2831.0
76	2125.0
77	1547.5
78	970.0
79	785.0
80	600.0
81	447.5
82	295.0
83	178.0
84	61.0
85	73.5
86	86.0
87	59.0
88	32.0
89	27.5
90	23.0
91	14.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	893562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.59918381597428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.54604735426435	19.60361008143083
2	12.919041310788629	7.3894807434681855
3	5.16568130545672	4.43202807568496
4	2.6370094098947425	3.0166526735213437
5	1.579853931045324	2.2591266488177384
6	1.0179060413016394	1.7466769189545772
7	0.7131174676010611	1.4276204276813378
8	0.5584667114123242	1.2777353707826975
9	0.4663562132178869	1.2003666358986036
>10	5.862024022674882	40.90291866788441
>50	0.4484534432515893	7.883823620792139
>100	0.07454398317549515	4.208909144561649
>500	0.006740679329699027	1.1724299936852807
>1k	0.003568594939252426	1.4710089832755064
>5k	0.0011895316464174753	2.0076120135607125
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	6488	0.7260828011934258	No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	5957	0.6666577137344695	No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	5259	0.588543380313845	No Hit
TCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	2292	0.2565015074499587	No Hit
ACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1862	0.20837949688997515	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1451	0.16238380772682812	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCC	1394	0.1560048435363187	No Hit
GCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	1348	0.1508569075229251	No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	1198	0.1340701596531634	No Hit
AGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	1181	0.1321676615612571	No Hit
GGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	1137	0.12724354885279365	No Hit
ACCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG	1109	0.12411002258377146	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1191165246507797E-4	0.0	0.0	0.7006788560838532	0.0
2	1.1191165246507797E-4	0.0	0.0	2.586166376815487	0.0
3	1.1191165246507797E-4	0.0	0.0	3.653020159765075	0.0
4	1.1191165246507797E-4	0.0	0.0	4.798771657702543	0.0
5	1.1191165246507797E-4	0.0	0.0	7.4382079810914075	0.0
6	1.1191165246507797E-4	0.0	0.0	8.878622859969425	0.0
7	1.1191165246507797E-4	0.0	0.0	10.368614600889474	0.0
8	1.1191165246507797E-4	0.0	0.0	12.736105608788199	0.0
9	1.1191165246507797E-4	0.0	0.0	13.622222073006686	0.0
10	1.1191165246507797E-4	0.0	0.0	15.3587551843073	0.0
11	1.1191165246507797E-4	0.0	0.0	17.94212376981116	0.0
12	1.1191165246507797E-4	0.0	0.0	19.87271168648622	0.0
13	1.1191165246507797E-4	0.0	0.0	20.66124118975516	0.0
14	1.1191165246507797E-4	0.0	0.0	20.989142331477836	0.0
15	1.1191165246507797E-4	0.0	0.0	21.566718369850104	0.0
16	1.1191165246507797E-4	0.0	0.0	22.743581307172864	0.0
17	1.1191165246507797E-4	0.0	0.0	24.128040359818346	0.0
18	3.357349573952339E-4	0.0	0.0	25.70565892461855	0.0
19	3.357349573952339E-4	0.0	0.0	26.579129372108483	0.0
20	3.357349573952339E-4	0.0	0.0	27.43547733677126	0.0
21	3.357349573952339E-4	0.0	0.0	28.408437243302647	0.0
22	3.357349573952339E-4	0.0	0.0	29.4238116661183	0.0
23	3.357349573952339E-4	0.0	0.0	30.39598819108243	0.0
24	3.357349573952339E-4	0.0	0.0	31.18451769435137	0.0
25	3.357349573952339E-4	0.0	0.0	31.86370951316193	0.0
26	4.476466098603119E-4	0.0	0.0	32.4762019871033	0.0
27	4.476466098603119E-4	0.0	0.0	33.070229038387936	0.0
28	4.476466098603119E-4	0.0	0.0	33.70767781082902	0.0
29	4.476466098603119E-4	0.0	0.0	34.374223612911024	0.0
30	4.476466098603119E-4	0.0	0.0	35.060465865826885	0.0
31	4.476466098603119E-4	0.0	0.0	35.71246315308843	0.0
32	4.476466098603119E-4	0.0	0.0	36.33558723401398	0.0
33	4.476466098603119E-4	0.0	0.0	36.907120043153135	0.0
34	4.476466098603119E-4	0.0	0.0	37.47182624149192	0.0
35	4.476466098603119E-4	0.0	0.0	38.0673081442586	0.0
36	4.476466098603119E-4	0.0	0.0	38.63268581251217	0.0
37	4.476466098603119E-4	0.0	0.0	39.18407452420761	0.0
38	4.476466098603119E-4	0.0	0.0	39.71565487341673	0.0
39	4.476466098603119E-4	0.0	0.0	40.292559441874204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.032442E-4	45.000004	1
GTCGACT	20	7.032442E-4	45.000004	33
CCCTATA	20	7.032442E-4	45.000004	4
CACGACA	20	7.032442E-4	45.000004	14
TAACGCG	35	1.2115197E-7	45.000004	1
ATCGTTC	20	7.032442E-4	45.000004	22
TAGTTCG	20	7.032442E-4	45.000004	1
TCTCGAT	20	7.032442E-4	45.000004	44
TCGTAGT	20	7.032442E-4	45.000004	41
TTACGAG	30	2.1649576E-6	45.000004	1
ACCCGTC	20	7.032442E-4	45.000004	17
AACTCGC	20	7.032442E-4	45.000004	39
CGCACGA	20	7.032442E-4	45.000004	39
ATACGAC	20	7.032442E-4	45.000004	2
CCGCACG	20	7.032442E-4	45.000004	38
GTACGAG	35	1.2115197E-7	45.000004	1
TGGTACG	20	7.032442E-4	45.000004	1
TAATCGA	20	7.032442E-4	45.000004	33
ACGATAT	25	3.8901067E-5	45.0	15
GTATGCG	50	2.1827873E-11	45.0	1
>>END_MODULE