FastQCFastQC Report
Sat 18 Jun 2016
SRR3549981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470021
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT65610.4463201546100362No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC65240.4438031837640415No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC51630.35121947237488443No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29290.19924885426806827No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC26000.17686822161043958No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC24420.16612007583565133No Hit
ACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC18320.12462406999627895No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17690.12033841693417985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT900.042.528
CGGTCTA900.042.531
TCTAGCG501.0822987E-940.51
TCGACAC501.0822987E-940.534
TATACGG950.040.263162
TCGATTG451.9288564E-840.01
TACTCGT403.4590994E-739.37511
CGAATAT4000.038.2514
CGTAAGG1950.038.0769232
CGTTTTT19450.038.0591241
AGGGCGT2150.037.674426
TATAGCG900.037.51
GCAACCG301.14007715E-437.4999961
GTATACG552.750312E-936.818181
TAAGGGT3250.036.6923074
AATAGCG800.036.56251
GCGTAAG800.036.56251
GCTACGA4500.036.510
CTACGAA4400.036.3068211
GGCGATA4400.036.306828