##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549981_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1470021
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32387428478913	33.0	31.0	34.0	30.0	34.0
2	32.482262498290844	34.0	31.0	34.0	31.0	34.0
3	32.4863508752596	34.0	31.0	34.0	31.0	34.0
4	36.0160990897409	37.0	35.0	37.0	35.0	37.0
5	35.90908565251789	37.0	35.0	37.0	35.0	37.0
6	35.86419649787316	37.0	35.0	37.0	35.0	37.0
7	36.08182128010416	37.0	35.0	37.0	35.0	37.0
8	36.054255007241395	37.0	35.0	37.0	35.0	37.0
9	37.59511666840134	39.0	38.0	39.0	35.0	39.0
10	37.38499518034096	39.0	37.0	39.0	34.0	39.0
11	37.319162107207994	39.0	37.0	39.0	34.0	39.0
12	37.218930886021354	39.0	37.0	39.0	34.0	39.0
13	37.15374134111009	39.0	37.0	39.0	33.0	39.0
14	38.30355076560131	40.0	38.0	41.0	34.0	41.0
15	38.393224994744976	40.0	38.0	41.0	34.0	41.0
16	38.42621091807532	40.0	38.0	41.0	34.0	41.0
17	38.44923984079139	40.0	38.0	41.0	34.0	41.0
18	38.4110465088594	40.0	38.0	41.0	34.0	41.0
19	38.38261698302269	40.0	37.0	41.0	34.0	41.0
20	38.35279359954722	40.0	37.0	41.0	34.0	41.0
21	38.330833369047106	40.0	37.0	41.0	34.0	41.0
22	38.23487011410041	40.0	37.0	41.0	34.0	41.0
23	37.98923960950218	40.0	37.0	41.0	34.0	41.0
24	37.812284314305714	40.0	36.0	41.0	33.0	41.0
25	37.79965524302034	40.0	36.0	41.0	33.0	41.0
26	37.878575884290086	40.0	36.0	41.0	33.0	41.0
27	37.95339454334326	40.0	37.0	41.0	34.0	41.0
28	37.95950602066229	40.0	37.0	41.0	34.0	41.0
29	37.94130900170814	40.0	36.0	41.0	34.0	41.0
30	37.88063571880946	40.0	36.0	41.0	33.0	41.0
31	37.76253196382909	40.0	36.0	41.0	33.0	41.0
32	37.64419351832389	40.0	36.0	41.0	33.0	41.0
33	37.58086313052671	40.0	36.0	41.0	33.0	41.0
34	37.532410081216526	40.0	36.0	41.0	33.0	41.0
35	37.43740871729043	40.0	36.0	41.0	33.0	41.0
36	37.37134775625654	40.0	36.0	41.0	33.0	41.0
37	37.32384639403111	40.0	35.0	41.0	33.0	41.0
38	37.25118688780636	39.0	35.0	41.0	32.0	41.0
39	37.23293612812334	39.0	35.0	41.0	32.0	41.0
40	37.14968153516174	39.0	35.0	41.0	32.0	41.0
41	37.096448962293735	39.0	35.0	41.0	32.0	41.0
42	37.03894094029949	39.0	35.0	41.0	32.0	41.0
43	36.95606117191523	39.0	35.0	41.0	32.0	41.0
44	36.88387376778971	39.0	35.0	41.0	32.0	41.0
45	36.83514861352321	39.0	35.0	41.0	31.0	41.0
46	36.7389710759234	39.0	35.0	41.0	31.0	41.0
47	36.64488398465056	39.0	35.0	41.0	31.0	41.0
48	36.58655964778735	39.0	35.0	41.0	31.0	41.0
49	36.56493410638351	39.0	35.0	41.0	31.0	41.0
50	36.47675033213811	39.0	35.0	40.0	31.0	41.0
51	35.50118739800316	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	11.0
10	3.0
11	15.0
12	14.0
13	15.0
14	32.0
15	40.0
16	65.0
17	131.0
18	290.0
19	487.0
20	858.0
21	1422.0
22	2221.0
23	3328.0
24	4755.0
25	6562.0
26	8452.0
27	10342.0
28	12316.0
29	15183.0
30	18831.0
31	24399.0
32	32642.0
33	46369.0
34	84800.0
35	113507.0
36	109973.0
37	174104.0
38	319367.0
39	479127.0
40	355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.422183084459334	20.98194515588553	25.22147642788776	11.374395331767369
2	30.699085251163076	24.499445926282686	26.433023745919275	18.36844507663496
3	31.97022355462949	24.365842392727725	26.669346900486456	16.994587152156328
4	28.543401760927225	25.452765640762955	26.585402521460576	19.41843007684924
5	25.68582353585425	29.764880909864555	24.221898870832455	20.32739668344874
6	26.23459120652018	34.47107218196203	23.791292777450117	15.503043834067675
7	79.52464624655022	6.246849534802564	8.820282159234461	5.408222059412757
8	79.6249849491946	5.42944624600601	8.880281302103848	6.06528750269554
9	72.82698682535828	7.870159677990994	11.66507145136022	7.6377820452905105
10	36.816140721799215	29.066115382025153	18.469327989191992	15.648415906983642
11	28.273677722971303	24.535431806756502	26.559076366936257	20.631814103335937
12	26.3144540112012	22.110024278564726	30.905476860534648	20.670044849699426
13	23.956528512177716	24.28434695830876	31.679071251363077	20.080053278150448
14	19.30353375904154	27.899601434265225	30.0901823851496	22.70668242154364
15	18.14082928067014	26.13622526480914	34.718755718455725	21.004189736064998
16	20.71677887594803	25.48405771074019	31.049080251234507	22.750083162077274
17	20.26596898955865	25.370249812757777	28.52292586296386	25.84085533471971
18	20.318961429802705	25.62942978365615	31.286968009300548	22.764640777240597
19	21.919142651703616	27.31103841373695	28.185855848317814	22.583963086241628
20	23.710545631661045	26.40050720363859	28.731562338225103	21.157384826475266
21	22.421516427316345	27.29396382772763	28.950878933022047	21.33364081193398
22	20.403790149936636	25.29800594685382	27.976267005709442	26.321936897500102
23	20.17889540353505	26.68961871973258	28.74918113414706	24.38230474258531
24	21.76662782368415	24.27053763177533	29.882362224757337	24.080472319783187
25	20.556985240346908	27.572463250524994	27.15675490350138	24.713796605626722
26	19.572169377172163	28.68360383967304	27.40198949538816	24.34223728776664
27	20.79745799549802	28.11089093285062	28.467280399395655	22.624370672255704
28	18.968572557806997	26.930499632318178	29.801547052729177	24.29938075714565
29	20.33692035692007	27.414166192183647	27.963205967805905	24.285707483090377
30	21.666017016083444	28.832649329499375	26.99247153612091	22.508862118296268
31	21.476359861525786	29.433116941866817	25.609702174322678	23.480821022284715
32	22.664097995878972	30.18562319858016	24.529241419000137	22.62103738654074
33	22.349204535173307	29.694065594981296	25.03569676895772	22.921033100887676
34	19.96039512360708	27.253488215474476	27.86368358003049	24.92243308088796
35	20.8942593337102	29.08067299718848	25.548818690345236	24.476248978756086
36	21.769484925725553	30.80336947567416	25.61698098190434	21.81016461669595
37	20.811471400748697	29.652161431707434	27.87191475495928	21.66445241258458
38	21.360919333805438	29.588692950644923	26.622204716803367	22.428182998746276
39	23.52558228759997	26.577783582683512	26.38186801413041	23.514766115586102
40	21.723431161867758	26.643020745962133	28.045925874528322	23.587622217641787
41	19.33054017595667	28.01769498530973	25.873439903239476	26.77832493549412
42	21.728601156037907	27.337228515783107	27.33960943415094	23.594560894028042
43	23.032187975545927	25.292767926444586	28.591224207001126	23.083819891008357
44	21.472550392137254	25.226442343340672	27.797153918209332	25.503853346312738
45	21.3539126311801	25.334331958523045	26.935329495292926	26.37642591500394
46	21.817715529233936	27.394914766523744	26.982063521541527	23.805306182700793
47	19.459994108927695	26.79723622995862	29.89787220726779	23.8448974538459
48	20.212568391880115	25.488683494997687	29.516653163458205	24.782094949663986
49	21.690030278479014	24.188021803770148	30.089774227715115	24.032173690035723
50	20.29630869218875	23.972310599644494	29.68848744337666	26.042893264790095
51	19.9535244734599	23.92258341887633	27.403350020169782	28.72054208749399
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	775.0
1	1491.0
2	2207.0
3	3860.5
4	5514.0
5	3886.5
6	2259.0
7	2230.5
8	2202.0
9	2308.0
10	2414.0
11	2509.5
12	2605.0
13	2785.5
14	2966.0
15	2938.5
16	2911.0
17	2815.5
18	2720.0
19	3135.5
20	3551.0
21	3562.0
22	3573.0
23	4296.0
24	5019.0
25	6499.5
26	10478.5
27	12977.0
28	15318.0
29	17659.0
30	19941.0
31	22223.0
32	25447.5
33	28672.0
34	32637.0
35	36602.0
36	41669.5
37	46737.0
38	51112.5
39	55488.0
40	63314.5
41	71141.0
42	84921.5
43	98702.0
44	118490.5
45	138279.0
46	157459.0
47	176639.0
48	170296.0
49	163953.0
50	138314.0
51	112675.0
52	97448.5
53	82222.0
54	73829.0
55	65436.0
56	62381.5
57	59327.0
58	57757.0
59	56187.0
60	52144.0
61	48101.0
62	43916.0
63	39731.0
64	35723.5
65	31716.0
66	26182.0
67	20648.0
68	16837.0
69	13026.0
70	11409.5
71	9793.0
72	8128.0
73	6463.0
74	5228.5
75	3171.5
76	2349.0
77	1811.0
78	1273.0
79	986.0
80	699.0
81	540.5
82	382.0
83	248.5
84	115.0
85	91.5
86	68.0
87	49.0
88	30.0
89	18.5
90	7.0
91	5.0
92	3.0
93	4.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1470021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.667928168325673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.52738424605508	18.960107448831042
2	12.960099401940518	7.171581986145014
3	5.116960477304673	4.2472708477868135
4	2.435286281087452	2.695173035777463
5	1.51999252739598	2.1027522032191883
6	0.9476838530105389	1.573226926282662
7	0.6739516786090783	1.3052792642874944
8	0.5026784552041222	1.1126457112282055
9	0.44178439499352046	1.1000933015911145
>10	6.277693725835077	43.39034489001371
>50	0.5120342934399059	8.648472126414616
>100	0.07498421906817797	3.9516375605732943
>500	0.005978808035279527	1.1664953823809594
>1k	0.0027402870161697835	1.3171665538466162
>5k	7.473510044099409E-4	1.2577527616218238
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	6561	0.4463201546100362	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	6524	0.4438031837640415	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	5163	0.35121947237488443	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2929	0.19924885426806827	No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	2600	0.17686822161043958	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	2442	0.16612007583565133	No Hit
ACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	1832	0.12462406999627895	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1769	0.12033841693417985	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4827141925183382	0.0
2	0.0	0.0	0.0	1.672629166522111	0.0
3	0.0	0.0	0.0	2.3854080996121825	0.0
4	0.0	0.0	0.0	3.2070290152317553	0.0
5	0.0	0.0	0.0	5.13366815848209	0.0
6	0.0	0.0	0.0	6.217530225758679	0.0
7	0.0	0.0	0.0	7.3420719840056705	0.0
8	0.0	0.0	0.0	9.14435916221605	0.0
9	0.0	0.0	0.0	9.798499477218353	0.0
10	0.0	0.0	0.0	11.24324074281932	0.0
11	0.0	0.0	0.0	13.235185075587356	0.0
12	0.0	0.0	0.0	14.79142134704198	0.0
13	0.0	0.0	0.0	15.425221816559084	0.0
14	0.0	0.0	0.0	15.67222509066197	0.0
15	0.0	0.0	0.0	16.158408621373436	0.0
16	6.80262390809383E-5	0.0	0.0	17.11057188978933	0.0
17	6.80262390809383E-5	0.0	0.0	18.3033439658345	0.0
18	6.80262390809383E-5	0.0	0.0	19.618563272225362	0.0
19	6.80262390809383E-5	0.0	0.0	20.387327800079046	0.0
20	6.80262390809383E-5	0.0	0.0	21.176092042222525	0.0
21	6.80262390809383E-5	0.0	0.0	22.11968400451422	0.0
22	1.360524781618766E-4	0.0	0.0	23.10205092308205	0.0
23	1.360524781618766E-4	0.0	0.0	24.039044340182894	0.0
24	2.040787172428149E-4	0.0	0.0	24.801074270367565	0.0
25	2.721049563237532E-4	0.0	0.0	25.46997627925043	0.0
26	2.721049563237532E-4	0.0	0.0	26.072484678790303	0.0
27	2.721049563237532E-4	0.0	0.0	26.672952291157745	0.0
28	2.721049563237532E-4	0.0	0.0	27.292603302946013	0.0
29	2.721049563237532E-4	0.0	0.0	27.975110559645067	0.0
30	2.721049563237532E-4	0.0	0.0	28.71571222451924	0.0
31	2.721049563237532E-4	0.0	0.0	29.365090702785878	0.0
32	2.721049563237532E-4	0.0	0.0	29.989367498831648	0.0
33	2.721049563237532E-4	0.0	0.0	30.59690984006351	0.0
34	2.721049563237532E-4	0.0	0.0	31.20173113173213	0.0
35	2.721049563237532E-4	0.0	0.0	31.84383080241711	0.0
36	2.721049563237532E-4	0.0	0.0	32.438788289418994	0.0
37	2.721049563237532E-4	0.0	0.0	33.04925575893134	0.0
38	3.401311954046915E-4	0.0	0.0	33.61489393688934	0.0
39	3.401311954046915E-4	0.0	0.0	34.23195995159253	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	90	0.0	42.5	28
CGGTCTA	90	0.0	42.5	31
TCTAGCG	50	1.0822987E-9	40.5	1
TCGACAC	50	1.0822987E-9	40.5	34
TATACGG	95	0.0	40.26316	2
TCGATTG	45	1.9288564E-8	40.0	1
TACTCGT	40	3.4590994E-7	39.375	11
CGAATAT	400	0.0	38.25	14
CGTAAGG	195	0.0	38.076923	2
CGTTTTT	1945	0.0	38.059124	1
AGGGCGT	215	0.0	37.67442	6
TATAGCG	90	0.0	37.5	1
GCAACCG	30	1.14007715E-4	37.499996	1
GTATACG	55	2.750312E-9	36.81818	1
TAAGGGT	325	0.0	36.692307	4
AATAGCG	80	0.0	36.5625	1
GCGTAAG	80	0.0	36.5625	1
GCTACGA	450	0.0	36.5	10
CTACGAA	440	0.0	36.30682	11
GGCGATA	440	0.0	36.30682	8
>>END_MODULE