FastQCFastQC Report
Sat 18 Jun 2016
SRR3549979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956778
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC55460.5796537963874587Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT54430.5688884986903963Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC44640.4665659118416184Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC18110.18928110805223364Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC17340.1812332641427792No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16140.16869116973843462No Hit
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC15290.1598071862020239Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG12440.13001971199170548No Hit
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG11080.1158053383334483No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10830.11319240199920985No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG9830.10274065666225603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG302.1651194E-645.0000041
TTGTGCG351.211647E-745.0000041
GTTAGCG600.045.0000041
CGCGATC302.1651194E-645.00000428
TAGTGCG302.1651194E-645.0000041
ACACGAC253.890315E-545.026
TTATGCG207.0326927E-445.01
TAAACCG253.890315E-545.040
AATTGCG253.890315E-545.01
TTAGTCG207.0326927E-445.01
TATACGG207.0326927E-445.02
ATCGCGT253.890315E-545.010
ATCGCCC207.0326927E-445.028
ATACCCG207.0326927E-445.014
GCGTAAT207.0326927E-445.021
ACGACAT207.0326927E-445.033
CGTAAGG1600.043.593752
TACGCGC700.041.78571734
CATACGC700.041.78571732
AACGGGA2450.041.326534
Produced by FastQC (version 0.11.5)