##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	956778
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27082771552021	33.0	31.0	34.0	30.0	34.0
2	32.435059125523374	34.0	31.0	34.0	30.0	34.0
3	32.43685996124493	34.0	31.0	34.0	30.0	34.0
4	35.979275234171354	37.0	35.0	37.0	35.0	37.0
5	35.85503115665285	37.0	35.0	37.0	35.0	37.0
6	35.80725727389217	37.0	35.0	37.0	35.0	37.0
7	36.03460154811252	37.0	35.0	37.0	35.0	37.0
8	35.9942275010504	37.0	35.0	37.0	35.0	37.0
9	37.508911158074284	39.0	38.0	39.0	35.0	39.0
10	37.34968404373846	39.0	37.0	39.0	34.0	39.0
11	37.270670939340164	39.0	37.0	39.0	34.0	39.0
12	37.13693145118303	39.0	37.0	39.0	34.0	39.0
13	37.04296607990568	39.0	37.0	39.0	33.0	39.0
14	38.168939921277456	40.0	37.0	41.0	33.0	41.0
15	38.26776639931102	40.0	37.0	41.0	34.0	41.0
16	38.29355608092995	40.0	37.0	41.0	34.0	41.0
17	38.31298169481322	40.0	37.0	41.0	34.0	41.0
18	38.272414290462365	40.0	37.0	41.0	34.0	41.0
19	38.236589888145424	40.0	37.0	41.0	34.0	41.0
20	38.20255482463016	40.0	37.0	41.0	34.0	41.0
21	38.18761405467099	40.0	37.0	41.0	34.0	41.0
22	38.081766094120056	40.0	37.0	41.0	34.0	41.0
23	37.83446525735332	40.0	36.0	41.0	33.0	41.0
24	37.64841582895928	39.0	36.0	41.0	33.0	41.0
25	37.65441617595722	39.0	36.0	41.0	33.0	41.0
26	37.739329290598235	40.0	36.0	41.0	33.0	41.0
27	37.83493349554442	40.0	36.0	41.0	33.0	41.0
28	37.83190980561844	40.0	36.0	41.0	33.0	41.0
29	37.834178879531095	40.0	36.0	41.0	33.0	41.0
30	37.787672793479786	40.0	36.0	41.0	33.0	41.0
31	37.652847368982144	40.0	36.0	41.0	33.0	41.0
32	37.523852973207994	40.0	36.0	41.0	33.0	41.0
33	37.46805842107574	40.0	36.0	41.0	33.0	41.0
34	37.41512033094406	40.0	36.0	41.0	33.0	41.0
35	37.3456622121328	40.0	35.0	41.0	33.0	41.0
36	37.27071170114697	40.0	35.0	41.0	33.0	41.0
37	37.19942034620361	39.0	35.0	41.0	32.0	41.0
38	37.10441502626524	39.0	35.0	41.0	32.0	41.0
39	37.055881301618555	39.0	35.0	41.0	32.0	41.0
40	37.00138903695528	39.0	35.0	41.0	32.0	41.0
41	36.91421102909975	39.0	35.0	41.0	32.0	41.0
42	36.8689445200454	39.0	35.0	41.0	32.0	41.0
43	36.78529815693923	39.0	35.0	41.0	31.0	41.0
44	36.70789566649735	39.0	35.0	41.0	31.0	41.0
45	36.66729586173595	39.0	35.0	41.0	31.0	41.0
46	36.56889058903946	39.0	35.0	41.0	31.0	41.0
47	36.44500709673508	39.0	35.0	40.0	31.0	41.0
48	36.38476846248555	39.0	35.0	40.0	31.0	41.0
49	36.34553574601423	39.0	35.0	40.0	31.0	41.0
50	36.25708262522759	38.0	35.0	40.0	31.0	41.0
51	35.27354098860969	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	3.0
11	10.0
12	14.0
13	13.0
14	15.0
15	26.0
16	51.0
17	104.0
18	174.0
19	381.0
20	664.0
21	1082.0
22	1637.0
23	2414.0
24	3422.0
25	4621.0
26	5766.0
27	7142.0
28	8551.0
29	10104.0
30	12794.0
31	17114.0
32	22116.0
33	31727.0
34	59272.0
35	79382.0
36	72697.0
37	115125.0
38	205598.0
39	294594.0
40	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.73484131115055	22.2805081220513	24.64563357435058	12.339016992447569
2	31.066558804654786	25.296672791389437	25.635936445026957	18.00083195892882
3	31.96729021779347	24.228190865592644	26.62832966477072	17.176189251843166
4	29.1852446440031	26.258546914749292	25.971437470343172	18.584770970904433
5	25.363877513906047	30.23679474235403	24.472761706477364	19.926566037262564
6	26.620281820861262	34.364816080637304	23.751068690960704	15.263833407540725
7	78.18929783084478	6.891776357733978	9.21990263154044	5.699023179880808
8	77.43792185857117	6.273555621053159	9.640167311539354	6.648355208836324
9	70.68013687605693	8.653731586637653	12.727299331715402	7.938832205590011
10	37.7218121654135	29.308993308792637	18.338423333312427	14.630771192481431
11	29.548442794462247	25.095163141292964	25.65297278992619	19.703421274318597
12	27.06563068966887	22.114638923553844	30.680889401721195	20.138840985056095
13	24.662669919249815	24.926158419194422	31.301200487469405	19.10997117408636
14	19.285874048107292	29.094209942118233	29.527957373601815	22.091958636172652
15	18.732767684875697	26.772354715513945	34.48386146002521	20.01101613958515
16	21.00403646404913	25.17072926007914	31.348024306578953	22.477209969292772
17	20.607392728511734	25.756131516401926	27.586754712169387	26.049721042916957
18	20.68525823127204	26.338189214216882	30.20251301764882	22.77403953686226
19	22.199820648050018	27.167848759064277	28.078091260459587	22.55423933242612
20	24.0615900449216	26.511165599543467	28.519050396225666	20.908193959309266
21	23.069823929898053	26.759080998936014	28.970356759875333	21.2007383112906
22	20.771589647755277	25.99683521151197	27.560625348827	25.670949791905752
23	20.668430921279544	26.76880112209938	28.229850602752155	24.332917353868922
24	22.522256991695045	25.044890246222216	28.60830830140325	23.82454446067949
25	20.933487183024692	27.09907627474712	26.795662107615353	25.171774434612836
26	20.12452209394447	27.376779148349982	28.982167232106086	23.51653152559946
27	22.821804013052137	26.614115291112462	27.720432535029026	22.843648160806374
28	19.036913474181052	26.632719397812245	28.96147277633892	25.368894351667787
29	20.892516341303836	23.9305251583962	28.335308713202018	26.84164978709795
30	22.974817564785145	25.259778130349986	26.49277052775043	25.27263377711444
31	22.957990254792648	27.21216415929296	24.08510647192975	25.744739113984643
32	23.05707280058697	27.081935412394515	24.362286758265764	25.49870502875275
33	23.30624240941995	26.91178099830891	23.777616124116566	26.004360468154573
34	20.164656796038372	26.416577304244036	26.665328843263538	26.753437056454054
35	22.221455760897513	24.605916942070156	26.496219603711623	26.67640769332071
36	22.90301407432027	27.76014916730945	25.458674844112217	23.87816191425806
37	22.183306890417633	28.253053477400194	26.5584074884665	23.005232143715677
38	21.363158433826865	27.113708718218852	24.30866930468719	27.214463543267094
39	21.69730073224928	26.356793320916662	25.694675253820638	26.251230693013426
40	22.51859888082711	25.08774240210373	28.576744030485653	23.816914686583512
41	21.107299707978235	26.29366477908146	26.234194348114194	26.364841164826114
42	22.813442616782574	25.70533603406433	27.375106869096072	24.106114480057027
43	23.736436247488967	23.78827690436026	27.252403378840235	25.222883469310542
44	20.61303667099369	24.429386963329005	26.572621862124755	28.38495450355255
45	21.073122500726395	23.035019617925997	26.844158205978818	29.047699675368786
46	22.9876732115496	24.39667300042434	27.385767649339765	25.229886138686297
47	20.85750299442504	24.585745073569836	29.71514813258666	24.841603799418465
48	20.860011413305905	23.398008733478402	28.528875036842404	27.213104816373285
49	21.577628248141156	23.176536249788352	29.726017947737095	25.519817554333397
50	20.20729991701314	22.51326849070526	29.544680166140946	27.73475142614065
51	20.317774865224745	22.044716747249623	26.560288802627152	31.07721958489848
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	697.0
1	1158.5
2	1620.0
3	2478.5
4	3337.0
5	2496.0
6	1655.0
7	1635.5
8	1616.0
9	1731.0
10	1846.0
11	1987.5
12	2129.0
13	2135.5
14	2142.0
15	2237.0
16	2332.0
17	2397.5
18	2463.0
19	2524.0
20	2585.0
21	2786.0
22	2987.0
23	3305.0
24	3623.0
25	4651.5
26	6606.0
27	7532.0
28	9130.0
29	10728.0
30	13159.5
31	15591.0
32	16898.0
33	18205.0
34	20151.0
35	22097.0
36	23505.0
37	24913.0
38	27286.0
39	29659.0
40	33514.5
41	37370.0
42	41568.0
43	45766.0
44	52400.5
45	59035.0
46	62795.0
47	66555.0
48	75622.5
49	84690.0
50	98573.0
51	112456.0
52	108246.5
53	104037.0
54	86230.0
55	68423.0
56	58264.0
57	48105.0
58	44016.5
59	39928.0
60	37231.5
61	34535.0
62	31843.0
63	29151.0
64	25706.5
65	22262.0
66	18529.5
67	14797.0
68	12263.0
69	9729.0
70	7672.0
71	5615.0
72	4833.5
73	4052.0
74	3534.5
75	2398.5
76	1780.0
77	1415.5
78	1051.0
79	775.0
80	499.0
81	387.5
82	276.0
83	207.0
84	138.0
85	84.5
86	31.0
87	32.5
88	34.0
89	19.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	956778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.040340003070597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.21208613298596	19.40730427490734
2	12.657916459246263	7.098645624954574
3	4.9817556714923645	4.1907036852261355
4	2.4860610680218542	2.7883999046291845
5	1.4465238974522918	2.0280510953564543
6	0.9652839937251794	1.6240134830145532
7	0.6893075615537482	1.3529892874858227
8	0.500695576194725	1.1231739355626742
9	0.42330041304750754	1.06825387547631
>10	6.006264377016928	41.939784571527184
>50	0.544357034470675	9.287196393483525
>100	0.07663277905190298	4.242892530136017
>500	0.006040021994238613	1.1413034767838326
>1k	0.0030200109971193064	1.5440728821967875
>5k	7.550027492798266E-4	1.163214979259649
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	5546	0.5796537963874587	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	5443	0.5688884986903963	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	4464	0.4665659118416184	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1811	0.18928110805223364	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	1734	0.1812332641427792	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1614	0.16869116973843462	No Hit
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1529	0.1598071862020239	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1244	0.13001971199170548	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1108	0.1158053383334483	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1083	0.11319240199920985	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	983	0.10274065666225603	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6012889092349532	0.0
2	0.0	0.0	0.0	2.092752968818263	0.0
3	0.0	0.0	0.0	2.9473921850209766	0.0
4	0.0	0.0	0.0	3.843106760397919	0.0
5	0.0	0.0	0.0	5.956345150076611	0.0
6	0.0	0.0	0.0	7.093495042737187	0.0
7	0.0	0.0	0.0	8.283844319162856	0.0
8	0.0	0.0	0.0	10.23602131319909	0.0
9	0.0	0.0	0.0	10.92656812761163	0.0
10	0.0	0.0	0.0	12.332223357978549	0.0
11	0.0	0.0	0.0	14.381392548741715	0.0
12	0.0	0.0	0.0	16.0005769363426	0.0
13	0.0	0.0	0.0	16.639178576430478	0.0
14	0.0	0.0	0.0	16.907474879230083	0.0
15	0.0	0.0	0.0	17.44249972302875	0.0
16	0.0	0.0	0.0	18.402492532227956	0.0
17	0.0	0.0	0.0	19.62628739373188	0.0
18	1.0451745336953818E-4	0.0	0.0	21.02692578633706	0.0
19	1.0451745336953818E-4	0.0	0.0	21.800354941271642	0.0
20	1.0451745336953818E-4	0.0	0.0	22.56385493813612	0.0
21	2.0903490673907635E-4	0.0	0.0	23.430827213836437	0.0
22	2.0903490673907635E-4	0.0	0.0	24.357687990317505	0.0
23	2.0903490673907635E-4	0.0	0.0	25.238247534955864	0.0
24	2.0903490673907635E-4	0.0	0.0	25.956491474511328	0.0
25	2.0903490673907635E-4	0.0	0.0	26.57837032206008	0.0
26	3.135523601086145E-4	0.0	0.0	27.156560874100364	0.0
27	3.135523601086145E-4	0.0	0.0	27.74018633371587	0.0
28	3.135523601086145E-4	0.0	0.0	28.337712614629517	0.0
29	3.135523601086145E-4	0.0	0.0	28.9800768830387	0.0
30	3.135523601086145E-4	0.0	0.0	29.67010111018439	0.0
31	3.135523601086145E-4	0.0	0.0	30.26731383873793	0.0
32	3.135523601086145E-4	0.0	0.0	30.867871125799297	0.0
33	3.135523601086145E-4	0.0	0.0	31.430906647101	0.0
34	3.135523601086145E-4	0.0	0.0	32.01714504305074	0.0
35	3.135523601086145E-4	0.0	0.0	32.60453313098754	0.0
36	3.135523601086145E-4	0.0	0.0	33.190039904763694	0.0
37	4.180698134781527E-4	0.0	0.0	33.74314626799529	0.0
38	4.180698134781527E-4	0.0	0.0	34.32583107053047	0.0
39	5.225872668476909E-4	0.0	0.0	34.92910581137944	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	30	2.1651194E-6	45.000004	1
TTGTGCG	35	1.211647E-7	45.000004	1
GTTAGCG	60	0.0	45.000004	1
CGCGATC	30	2.1651194E-6	45.000004	28
TAGTGCG	30	2.1651194E-6	45.000004	1
ACACGAC	25	3.890315E-5	45.0	26
TTATGCG	20	7.0326927E-4	45.0	1
TAAACCG	25	3.890315E-5	45.0	40
AATTGCG	25	3.890315E-5	45.0	1
TTAGTCG	20	7.0326927E-4	45.0	1
TATACGG	20	7.0326927E-4	45.0	2
ATCGCGT	25	3.890315E-5	45.0	10
ATCGCCC	20	7.0326927E-4	45.0	28
ATACCCG	20	7.0326927E-4	45.0	14
GCGTAAT	20	7.0326927E-4	45.0	21
ACGACAT	20	7.0326927E-4	45.0	33
CGTAAGG	160	0.0	43.59375	2
TACGCGC	70	0.0	41.785717	34
CATACGC	70	0.0	41.785717	32
AACGGGA	245	0.0	41.32653	4
>>END_MODULE