SRR3549977_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1289614
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	5850	0.4536241076787318	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	5214	0.40430702520289014	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	4237	0.3285479220914165	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2336	0.18113947274145595	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2167	0.16803477629740374	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	1961	0.15206100430051164	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1643	0.12740246306259081	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	30	2.1657088E-6	45.000004	1
TCGCAAA	20	7.033607E-4	45.0	2
ACGTTAC	20	7.033607E-4	45.0	40
TAGCCGT	25	3.891072E-5	45.0	44
TCTCGAA	25	3.891072E-5	45.0	12
ATGCGAA	35	1.2120836E-7	45.0	26
TCGGTAA	25	3.891072E-5	45.0	34
TAGTGCG	75	0.0	42.0	1
CGAATAT	155	0.0	40.64516	14
CGACAAT	50	1.0822987E-9	40.5	20
TACGCGC	75	0.0	39.0	34
ACTTACG	35	6.2488834E-6	38.571426	1
ACGGATA	35	6.2488834E-6	38.571426	12
TATCGTG	35	6.2488834E-6	38.571426	1
GCGTACG	35	6.2488834E-6	38.571426	1
TTTACGG	130	0.0	38.076927	2
AACGGGA	290	0.0	38.01724	4
TCGCCTC	30	1.139999E-4	37.500004	44
ATTAACG	30	1.139999E-4	37.500004	1
ATACCGG	30	1.139999E-4	37.500004	2