##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1289614
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22994865130186	33.0	31.0	34.0	30.0	34.0
2	32.39285553661794	34.0	31.0	34.0	30.0	34.0
3	32.40513285370661	34.0	31.0	34.0	30.0	34.0
4	35.95442279627858	37.0	35.0	37.0	35.0	37.0
5	35.83187604973271	37.0	35.0	37.0	35.0	37.0
6	35.78351739357668	37.0	35.0	37.0	35.0	37.0
7	35.99860190723736	37.0	35.0	37.0	35.0	37.0
8	35.964894146620615	37.0	35.0	37.0	35.0	37.0
9	37.49859958095988	39.0	37.0	39.0	35.0	39.0
10	37.322533719392005	39.0	37.0	39.0	34.0	39.0
11	37.20132303154277	39.0	37.0	39.0	34.0	39.0
12	37.019577951231916	39.0	37.0	39.0	33.0	39.0
13	36.93659265485641	39.0	37.0	39.0	33.0	39.0
14	38.03768259339616	40.0	37.0	41.0	33.0	41.0
15	38.13418821445797	40.0	37.0	41.0	33.0	41.0
16	38.16934834764511	40.0	37.0	41.0	34.0	41.0
17	38.176693956486204	40.0	37.0	41.0	34.0	41.0
18	38.134983801354515	40.0	37.0	41.0	34.0	41.0
19	38.08754867735617	40.0	37.0	41.0	34.0	41.0
20	38.045697394724314	40.0	36.0	41.0	34.0	41.0
21	38.01233392317391	40.0	36.0	41.0	34.0	41.0
22	37.90165351802943	40.0	36.0	41.0	33.0	41.0
23	37.63876865480679	39.0	36.0	41.0	33.0	41.0
24	37.43010699325534	39.0	35.0	41.0	32.0	41.0
25	37.440123944063885	39.0	35.0	41.0	33.0	41.0
26	37.537222765881886	39.0	35.0	41.0	33.0	41.0
27	37.618992194563646	39.0	36.0	41.0	33.0	41.0
28	37.608734861749326	39.0	36.0	41.0	33.0	41.0
29	37.586335135939905	39.0	36.0	41.0	33.0	41.0
30	37.53765622891811	39.0	36.0	41.0	33.0	41.0
31	37.38360238024711	39.0	35.0	41.0	33.0	41.0
32	37.26412476911696	39.0	35.0	41.0	32.0	41.0
33	37.21659581859378	39.0	35.0	41.0	32.0	41.0
34	37.158309385599104	39.0	35.0	41.0	32.0	41.0
35	37.07623676541973	39.0	35.0	41.0	32.0	41.0
36	37.01041319340516	39.0	35.0	41.0	32.0	41.0
37	36.95695611244915	39.0	35.0	41.0	32.0	41.0
38	36.83968071066226	39.0	35.0	41.0	31.0	41.0
39	36.76884478611429	39.0	35.0	41.0	31.0	41.0
40	36.721036682294084	39.0	35.0	41.0	31.0	41.0
41	36.64470221322039	39.0	35.0	41.0	31.0	41.0
42	36.59001453147996	39.0	35.0	41.0	31.0	41.0
43	36.48696199017691	39.0	35.0	41.0	31.0	41.0
44	36.391381452124435	39.0	35.0	40.0	31.0	41.0
45	36.33238783077727	38.0	35.0	40.0	31.0	41.0
46	36.20995119469857	38.0	35.0	40.0	30.0	41.0
47	36.07691526301669	38.0	35.0	40.0	30.0	41.0
48	36.01558140652939	38.0	35.0	40.0	30.0	41.0
49	35.96757169199466	38.0	35.0	40.0	30.0	41.0
50	35.89377829334979	38.0	35.0	40.0	30.0	41.0
51	34.831003695679485	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	13.0
11	20.0
12	15.0
13	26.0
14	24.0
15	53.0
16	93.0
17	186.0
18	326.0
19	558.0
20	967.0
21	1586.0
22	2545.0
23	3551.0
24	4819.0
25	6651.0
26	8695.0
27	10869.0
28	12923.0
29	15627.0
30	19679.0
31	25211.0
32	33284.0
33	46919.0
34	86491.0
35	110917.0
36	103539.0
37	161302.0
38	274086.0
39	358416.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48620129744249	21.230616292937267	24.725538029208742	11.557644380411503
2	31.830997492272882	24.800133993582577	25.603862861290278	17.765005652854267
3	32.62456828167188	23.665453383725673	26.369208150655933	17.340770183946514
4	29.288608839544235	25.861847033298336	25.494682905117344	19.354861222040086
5	25.557725024697316	30.234550803573786	23.920258309850855	20.28746586187805
6	27.007771317619074	33.87657081886518	23.456708751610947	15.658949111904802
7	78.05552669248318	7.1370192941453805	9.29192766207563	5.515526351295814
8	77.74558899019397	5.963257222703848	9.834105398979851	6.457048388122337
9	71.45075968468085	7.882048426893629	12.595706932461962	8.071484955963568
10	41.66890247779568	23.68189241121762	18.68155897811283	15.967646132873867
11	31.962044456713407	23.780759203916833	23.751215479980832	20.505980859388934
12	28.521324985615852	21.040559423207252	29.83582684431155	20.60228874686534
13	24.79796280127232	23.037280922818766	31.03479025506857	21.129966020840342
14	20.61283453808659	26.793443619563682	29.756578324987164	22.83714351736256
15	19.913865699348797	24.806802655678364	34.09857523258897	21.18075641238386
16	22.129489909383736	23.863807309784168	31.420719688216785	22.58598309261531
17	22.418025858900414	24.324797962801274	28.38298901841946	24.874187159878847
18	22.388249507216887	25.375732583548256	29.673995474614884	22.562022434619973
19	23.86838232215221	26.3579644761921	27.27040804457768	22.50324515707801
20	25.817957931598134	26.11936594981134	26.8822298765367	21.180446242053826
21	24.496709868224134	26.51188650247283	27.899200846144662	21.09220278315837
22	22.068386354366503	25.228169049033276	26.69418911395193	26.009255482648296
23	22.868160550366234	25.58176322527516	27.7956814984949	23.75439472586371
24	23.781534629741923	23.62846557186879	28.225189863013274	24.364809935376012
25	22.718270738375978	25.21855376880214	26.35494031547424	25.70823517734764
26	20.653156680991366	25.78407182304162	27.19550190987381	26.3672695860932
27	21.911052454455366	25.350996499727824	27.598180540844	25.13977050497281
28	18.990488626829425	26.13944947868122	28.496356274047898	26.373705620441466
29	20.546613172623747	25.417217865190672	27.03553156215736	27.000637400028225
30	23.086055207217044	24.366360787026196	25.884567009973523	26.663016995783234
31	23.482918144499052	25.810668928842272	24.81393657326921	25.892476353389466
32	22.756886944465553	26.368355182248333	24.060843011940005	26.81391486134611
33	22.60591153632017	25.982658376847645	24.432116896993985	26.9793131898382
34	21.54799808314736	24.59425843702069	27.365087537821392	26.492655942010558
35	21.048934022118246	24.900706723096988	25.061142326308495	28.989216928476274
36	21.306220310883724	27.23985626706906	24.645203913729226	26.808719508317992
37	22.12483735443319	27.447437760446146	26.78033892311963	23.64738596200103
38	20.551343270156806	27.910522063191003	25.28291411228476	26.25522055436743
39	23.057441994271155	25.956293898794524	25.50088631171808	25.48537779521624
40	23.68096190022751	23.378933541354236	27.01180353190955	25.92830102650871
41	20.27327556927887	24.71227824759967	25.18536554348821	29.829080639633254
42	20.864382675746388	25.19311980173913	26.22497894718885	27.717518575325638
43	21.681836580558215	24.174287810150943	27.55607491854152	26.587800690749326
44	20.78304050669425	24.185531484614774	27.14990687135841	27.881521137332566
45	21.687497189081384	22.482851457878095	26.6289758020617	29.200675550978822
46	22.62878659816038	23.57046372015192	26.590514681137144	27.210235000550554
47	20.426344627152	23.476094397238242	29.02628228291566	27.071278692694094
48	19.65758746415594	22.967957854055555	29.182530586671675	28.191924095116832
49	21.52527810647217	22.8915008677015	29.6378606311656	25.945360394660728
50	20.60608833340829	21.874917611006083	29.013875469714197	28.50511858587143
51	19.820892142920286	22.218198623774246	26.898746446611156	31.062162786694316
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	749.0
1	1562.0
2	2375.0
3	3602.0
4	4829.0
5	3483.5
6	2138.0
7	2174.5
8	2211.0
9	2329.0
10	2447.0
11	2443.5
12	2440.0
13	2682.5
14	2925.0
15	2847.5
16	2770.0
17	2741.5
18	2713.0
19	3066.0
20	3419.0
21	3322.0
22	3225.0
23	4045.5
24	4866.0
25	5915.0
26	8593.0
27	10222.0
28	11785.0
29	13348.0
30	15495.0
31	17642.0
32	18998.0
33	20354.0
34	23117.5
35	25881.0
36	27545.5
37	29210.0
38	31819.5
39	34429.0
40	39589.5
41	44750.0
42	49825.5
43	54901.0
44	61270.0
45	67639.0
46	73183.0
47	78727.0
48	94224.5
49	109722.0
50	124652.5
51	139583.0
52	137198.5
53	134814.0
54	117428.0
55	100042.0
56	88607.5
57	77173.0
58	71856.0
59	66539.0
60	63344.5
61	60150.0
62	55256.5
63	50363.0
64	43087.5
65	35812.0
66	30677.0
67	25542.0
68	21399.0
69	17256.0
70	14709.5
71	12163.0
72	10085.5
73	8008.0
74	6720.5
75	3921.5
76	2410.0
77	2165.5
78	1921.0
79	1319.0
80	717.0
81	592.0
82	467.0
83	328.0
84	189.0
85	127.0
86	65.0
87	44.0
88	23.0
89	22.0
90	21.0
91	16.0
92	11.0
93	10.5
94	10.0
95	7.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1289614.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.129509181262417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.72727679408698	19.613940727636955
2	12.041627398554176	6.774501369299416
3	4.787133626675153	4.039791579104663
4	2.455564317413351	2.762952760474369
5	1.4452523825711283	2.032712008238797
6	0.9348885229942339	1.5778773174613898
7	0.7037069813048614	1.385645239407349
8	0.5368361414303701	1.2080749735359269
9	0.435557382270621	1.1026813845193253
>10	6.3897301414378616	44.07584418645361
>50	0.45494996618744854	7.802143556749468
>100	0.07716862289187484	4.110018594077373
>500	0.00696467714994569	1.2487491422414434
>1k	0.0027858708599782763	1.398034810014895
>5k	5.571741719956553E-4	0.8670323507850223
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	5850	0.4536241076787318	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	5214	0.40430702520289014	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	4237	0.3285479220914165	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2336	0.18113947274145595	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2167	0.16803477629740374	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	1961	0.15206100430051164	No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1643	0.12740246306259081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1017033003673966E-4	0.0	0.0	0.4315244716636141	0.0
2	3.1017033003673966E-4	0.0	0.0	1.5870640362154878	0.0
3	3.1017033003673966E-4	0.0	0.0	2.25850525816252	0.0
4	3.877129125459246E-4	0.0	0.0	3.0587447096573084	0.0
5	3.877129125459246E-4	0.0	0.0	4.886035666486251	0.0
6	3.877129125459246E-4	0.0	0.0	5.9550377089578745	0.0
7	3.877129125459246E-4	0.0	0.0	7.048853377832437	0.0
8	3.877129125459246E-4	0.0	0.0	8.852106134083533	0.0
9	4.652554950551095E-4	0.0	0.0	9.503153656830648	0.0
10	4.652554950551095E-4	0.0	0.0	10.769501571788147	0.0
11	4.652554950551095E-4	0.0	0.0	12.70426654797482	0.0
12	5.427980775642944E-4	0.0	0.0	14.215338853331307	0.0
13	5.427980775642944E-4	0.0	0.0	14.833198150764492	0.0
14	5.427980775642944E-4	0.0	0.0	15.100254804926125	0.0
15	5.427980775642944E-4	0.0	0.0	15.588307819238935	0.0
16	5.427980775642944E-4	0.0	0.0	16.511839976923326	0.0
17	5.427980775642944E-4	0.0	0.0	17.596505621061805	0.0
18	6.203406600734793E-4	0.0	0.0	18.84447594396463	0.0
19	6.203406600734793E-4	0.0	0.0	19.58144064813192	0.0
20	6.203406600734793E-4	0.0	0.0	20.355315621573588	0.0
21	6.203406600734793E-4	0.0	0.0	21.24635743718663	0.0
22	6.203406600734793E-4	0.0	0.0	22.16469424184291	0.0
23	6.203406600734793E-4	0.0	0.0	23.02247028955951	0.0
24	6.203406600734793E-4	0.0	0.0	23.74958708574814	0.0
25	6.203406600734793E-4	0.0	0.0	24.376829035664937	0.0
26	6.203406600734793E-4	0.0	0.0	24.956925095416146	0.0
27	6.978832425826643E-4	0.0	0.0	25.540200401050235	0.0
28	6.978832425826643E-4	0.0	0.0	26.10672650886234	0.0
29	6.978832425826643E-4	0.0	0.0	26.72342266755789	0.0
30	6.978832425826643E-4	0.0	0.0	27.367181187549143	0.0
31	6.978832425826643E-4	0.0	0.0	27.997524840766307	0.0
32	7.754258250918492E-4	0.0	0.0	28.619028639577422	0.0
33	7.754258250918492E-4	0.0	0.0	29.201218349056383	0.0
34	7.754258250918492E-4	0.0	0.0	29.80201827833755	0.0
35	7.754258250918492E-4	0.0	0.0	30.406385166414136	0.0
36	7.754258250918492E-4	0.0	0.0	30.98655876874786	0.0
37	7.754258250918492E-4	0.0	0.0	31.58479979280622	0.0
38	7.754258250918492E-4	0.0	0.0	32.18040436905927	0.0
39	7.754258250918492E-4	0.0	0.0	32.802295880782935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	30	2.1657088E-6	45.000004	1
TCGCAAA	20	7.033607E-4	45.0	2
ACGTTAC	20	7.033607E-4	45.0	40
TAGCCGT	25	3.891072E-5	45.0	44
TCTCGAA	25	3.891072E-5	45.0	12
ATGCGAA	35	1.2120836E-7	45.0	26
TCGGTAA	25	3.891072E-5	45.0	34
TAGTGCG	75	0.0	42.0	1
CGAATAT	155	0.0	40.64516	14
CGACAAT	50	1.0822987E-9	40.5	20
TACGCGC	75	0.0	39.0	34
ACTTACG	35	6.2488834E-6	38.571426	1
ACGGATA	35	6.2488834E-6	38.571426	12
TATCGTG	35	6.2488834E-6	38.571426	1
GCGTACG	35	6.2488834E-6	38.571426	1
TTTACGG	130	0.0	38.076927	2
AACGGGA	290	0.0	38.01724	4
TCGCCTC	30	1.139999E-4	37.500004	44
ATTAACG	30	1.139999E-4	37.500004	1
ATACCGG	30	1.139999E-4	37.500004	2
>>END_MODULE