SRR3549976_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	789963
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	5955	0.7538327744463981	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	5740	0.7266163098778043	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	4944	0.6258520968703597	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2006	0.2539359438353442	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1694	0.21444042315905934	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1496	0.18937595811449398	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC	1476	0.18684419396857826	No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1229	0.15557690676651945	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1212	0.1534249072424911	No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1196	0.15139949592575855	No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	950	0.1202587969309955	No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	832	0.1053213884700929	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC	817	0.10342256536065614	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	802	0.10152374225121936	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTAT	60	0.0	45.000004	24
AGCGTGC	20	7.031946E-4	45.0	12
TCGCACT	20	7.031946E-4	45.0	19
CGACGGT	25	3.8896935E-5	45.0	28
GACACGA	20	7.031946E-4	45.0	37
ATGTGCG	20	7.031946E-4	45.0	1
CGCGACA	20	7.031946E-4	45.0	29
CGGTCTA	25	3.8896935E-5	45.0	31
CGGTAGT	50	2.1827873E-11	45.0	12
TCGACGC	20	7.031946E-4	45.0	16
TTCGATA	20	7.031946E-4	45.0	26
ATACCCG	25	3.8896935E-5	45.0	41
TGCGTAG	45	3.8380676E-10	45.0	1
GTACGCG	20	7.031946E-4	45.0	1
TACCGGT	20	7.031946E-4	45.0	18
AGCACGT	20	7.031946E-4	45.0	11
ACGGGTA	110	0.0	44.999996	5
CACTAGG	80	0.0	42.1875	2
ATTGGGT	65	0.0	41.538464	4
CGAAAGG	120	0.0	41.250004	2